BR112023024599A2 - Células sensíveis à toxina botulínica dentro das quais um gene específico foi inserido por um lentivírus - Google Patents

Células sensíveis à toxina botulínica dentro das quais um gene específico foi inserido por um lentivírus

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Publication number
BR112023024599A2
BR112023024599A2 BR112023024599A BR112023024599A BR112023024599A2 BR 112023024599 A2 BR112023024599 A2 BR 112023024599A2 BR 112023024599 A BR112023024599 A BR 112023024599A BR 112023024599 A BR112023024599 A BR 112023024599A BR 112023024599 A2 BR112023024599 A2 BR 112023024599A2
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BR
Brazil
Prior art keywords
botulinum toxin
cells
lentivirus
measure
specific gene
Prior art date
Application number
BR112023024599A
Other languages
English (en)
Inventor
Jin Choi Doo
Sup Lee Hak
Ho Lim Il
Su Jang Sung
Shik Ahn Yong
Original Assignee
Atgc Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020220063008A external-priority patent/KR20220159283A/ko
Application filed by Atgc Co Ltd filed Critical Atgc Co Ltd
Publication of BR112023024599A2 publication Critical patent/BR112023024599A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

células para medir a atividade da toxina botulínica, método para medir a atividade da toxina botulínica e kit para medir a atividade da toxina botulínica. a presente invenção refere-se a células para medir a atividade da toxina botulínica que superexpressam septina2 ou tiorredoxina (txn), e a um método para medir a atividade da toxina botulínica, o método compreendendo as etapas de: tratar as células para medir a atividade da toxina botulínica com toxina botulínica e cultivar a células; lisar as células cultivadas e coletar um lisado celular; e medir a quantidade de produtos de clivagem snap-25 no lisado celular.
BR112023024599A 2021-05-24 2022-05-24 Células sensíveis à toxina botulínica dentro das quais um gene específico foi inserido por um lentivírus BR112023024599A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20210066040 2021-05-24
KR1020220063008A KR20220159283A (ko) 2021-05-24 2022-05-23 렌티바이러스에 의해 특정 유전자가 삽입된 보툴리눔 독소에 민감한 세포
PCT/KR2022/007323 WO2022250405A1 (ko) 2021-05-24 2022-05-24 렌티바이러스에 의해 특정 유전자가 삽입된 보툴리눔 독소에 민감한 세포

Publications (1)

Publication Number Publication Date
BR112023024599A2 true BR112023024599A2 (pt) 2024-02-20

Family

ID=84229351

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023024599A BR112023024599A2 (pt) 2021-05-24 2022-05-24 Células sensíveis à toxina botulínica dentro das quais um gene específico foi inserido por um lentivírus

Country Status (4)

Country Link
EP (1) EP4349992A1 (pt)
BR (1) BR112023024599A2 (pt)
CA (1) CA3219818A1 (pt)
WO (1) WO2022250405A1 (pt)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6444209B1 (en) 1996-10-28 2002-09-03 Wisconsin Alumni Research Foundation Hybrid botulinal neurotoxins
EP3556770A1 (en) * 2008-03-14 2019-10-23 Allergan, Inc. Immuno-based botulinum toxin serotype a activity assays
KR101604515B1 (ko) 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
JP5717657B2 (ja) * 2009-03-13 2015-05-13 アラーガン、インコーポレイテッドＡｌｌｅｒｇａｎ，Ｉｎｃｏｒｐｏｒａｔｅｄ 免疫系ボツリヌス毒素血清型ａ活性アッセイに有用な細胞
KR102251096B1 (ko) * 2018-12-12 2021-05-13 휴젤(주) 보툴리눔 독소 활성을 결정하는 세포 기반 방법

Also Published As

Publication number Publication date
WO2022250405A1 (ko) 2022-12-01
EP4349992A1 (en) 2024-04-10
CA3219818A1 (en) 2022-12-01

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