BR112023024014A2 - Método para análise de sequência em tempo real de fragmentos de dna, oligonucleotídeo adaptador e kit para sequenciamento paralelo em tempo real e uso destes - Google Patents
Método para análise de sequência em tempo real de fragmentos de dna, oligonucleotídeo adaptador e kit para sequenciamento paralelo em tempo real e uso destesInfo
- Publication number
- BR112023024014A2 BR112023024014A2 BR112023024014A BR112023024014A BR112023024014A2 BR 112023024014 A2 BR112023024014 A2 BR 112023024014A2 BR 112023024014 A BR112023024014 A BR 112023024014A BR 112023024014 A BR112023024014 A BR 112023024014A BR 112023024014 A2 BR112023024014 A2 BR 112023024014A2
- Authority
- BR
- Brazil
- Prior art keywords
- real
- sequence
- dna fragments
- adapter oligonucleotide
- time
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 8
- 239000012634 fragment Substances 0.000 title abstract 6
- 238000012163 sequencing technique Methods 0.000 title abstract 6
- 238000012300 Sequence Analysis Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 108020004414 DNA Proteins 0.000 abstract 6
- 230000000295 complement effect Effects 0.000 abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus For Radiation Diagnosis (AREA)
Abstract
método para análise de sequência em tempo real de fragmentos de dna, oligonucleotídeo adaptador e kit para sequenciamento paralelo em tempo real e uso destes. a invenção está relacionada a um método para análise de sequência em tempo real de fragmentos de dna, compreendendo i) fornecer pelo menos uma amostra de fragmentos de dna para análise de sequência, ii) conectar um tipo de primeiro e segundo oligonucleotídeos adaptadores às extremidades 5' e 3' de uma fita de dna dos fragmentos de dna da amostra, respectivamente, em que um primeiro oligonucleotídeo adaptador compreende de 5' a 3' a) uma primeira sequência de ligação de células de fluxo, b) um sítio iniciador de sequenciamento de leitura 1, c) opcionalmente uma sequência aleatória, e d) uma sequência de código de barras específica da amostra, e um segundo oligonucleotídeo adaptador compreende de 5' a 3' d) uma sequência complementar à sequência de código de barras específica da amostra do primeiro oligonucleotídeo adaptador, c) opcionalmente uma sequência complementar à sequência aleatória, b) um sítio iniciador de sequenciamento de leitura 2 que pode ser (parcialmente) complementar ao sítio iniciador de sequenciamento de leitura 1, e a) uma segunda sequência de ligação de células de fluxo, em que o primeiro e o segundo oligonucleotídeos adaptadores de um tipo têm sequências de código de barras complementares, e sequenciamento dos fragmentos de dna compreendendo os oligonucleotídeos adaptadores conectados em um processo de sequenciamento por síntese.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21174771 | 2021-05-19 | ||
| EP21190984 | 2021-08-12 | ||
| PCT/EP2022/063044 WO2022243192A1 (en) | 2021-05-19 | 2022-05-13 | Method for parallel real-time sequence analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023024014A2 true BR112023024014A2 (pt) | 2024-02-06 |
Family
ID=81984815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023024014A BR112023024014A2 (pt) | 2021-05-19 | 2022-05-13 | Método para análise de sequência em tempo real de fragmentos de dna, oligonucleotídeo adaptador e kit para sequenciamento paralelo em tempo real e uso destes |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP4341436A1 (pt) |
| AU (1) | AU2022278434A1 (pt) |
| BR (1) | BR112023024014A2 (pt) |
| CA (1) | CA3218561A1 (pt) |
| WO (1) | WO2022243192A1 (pt) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5858988A (en) | 1993-02-24 | 1999-01-12 | Wang; Jui H. | Poly-substituted-phenyl-oligoribo nucleotides having enhanced stability and membrane permeability and methods of use |
| US6291438B1 (en) | 1993-02-24 | 2001-09-18 | Jui H. Wang | Antiviral anticancer poly-substituted phenyl derivatized oligoribonucleotides and methods for their use |
| US20190323073A1 (en) | 2016-06-23 | 2019-10-24 | Accuragen Holdings Limited | Cell-free nucleic acid standards and uses thereof |
| WO2018053362A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation |
| US20190309352A1 (en) | 2016-11-16 | 2019-10-10 | Progenity, Inc | Multimodal assay for detecting nucleic acid aberrations |
-
2022
- 2022-05-13 AU AU2022278434A patent/AU2022278434A1/en active Pending
- 2022-05-13 CA CA3218561A patent/CA3218561A1/en active Pending
- 2022-05-13 EP EP22728901.4A patent/EP4341436A1/en active Pending
- 2022-05-13 WO PCT/EP2022/063044 patent/WO2022243192A1/en active Application Filing
- 2022-05-13 BR BR112023024014A patent/BR112023024014A2/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4341436A1 (en) | 2024-03-27 |
| AU2022278434A1 (en) | 2023-11-23 |
| CA3218561A1 (en) | 2022-11-24 |
| WO2022243192A1 (en) | 2022-11-24 |
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