BR112022021086A2 - INTEGRATED SYSTEM FOR THE DETECTION AND IDENTIFICATION OF SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND METHOD OF USE OF THE INTEGRATED SYSTEM - Google Patents

INTEGRATED SYSTEM FOR THE DETECTION AND IDENTIFICATION OF SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND METHOD OF USE OF THE INTEGRATED SYSTEM

Info

Publication number
BR112022021086A2
BR112022021086A2 BR112022021086A BR112022021086A BR112022021086A2 BR 112022021086 A2 BR112022021086 A2 BR 112022021086A2 BR 112022021086 A BR112022021086 A BR 112022021086A BR 112022021086 A BR112022021086 A BR 112022021086A BR 112022021086 A2 BR112022021086 A2 BR 112022021086A2
Authority
BR
Brazil
Prior art keywords
integrated system
identification
detection
nucleic acids
specific sequences
Prior art date
Application number
BR112022021086A
Other languages
Portuguese (pt)
Inventor
Orfeu De Leão E Flores Alcino
Maria Reimão Pinto de França Doria Gonçalo
Original Assignee
Stab Vida Investig E Servicos Em Ciencias Biologicas Lda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stab Vida Investig E Servicos Em Ciencias Biologicas Lda filed Critical Stab Vida Investig E Servicos Em Ciencias Biologicas Lda
Publication of BR112022021086A2 publication Critical patent/BR112022021086A2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/023—Sending and receiving of information, e.g. using bluetooth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

Abstract

SISTEMA INTEGRADO PARA A DETECÇÃO E IDENTIFICAÇÃO DE SEQUÊNCIAS ESPECÍFICAS DE ÁCIDOS NUCLEICOS E MÉTODO DE UTILIZAÇÃO DO SISTEMA INTEGRADO. A presente invenção relaciona-se com um método e dispositivo portátil (1) para a detecção e identificação de sequências específicas de ácidos nucleicos em diferentes tipos de amostra, através da utilização de uma técnica de transcrição reversa e/ou amplificação isotérmica, utilizando oligonucleotídeos iniciadores específicos da(s) região(ões) alvo a ser detectada, sendo a amplificação das mesmas realizada de forma indireta e não específica, ou direta e específica, utilizando reagentes discriminatórios da amplificação, cujo sinal, colorimétrico ou de fluorescência, é registrado pelo dispositivo portátil (1) onde se executa a reação. Este dispositivo é controlado por via de uma aplicação móvel (9) que registra também os dados adquiridos pelo dispositivo (1), analisa-os e armazena-os em um servidor remoto em nuvem (10), que por sua vez analisa o conjunto global de todos os dados por forma a permitir uma análise global de, por exemplo, evolução de epidemias. Desta forma, a presente invenção é útil para a detecção e identificação de sequências nucleicas relevantes para diferentes tipos de diagnóstico em diferentes áreas clínicas, farmacêuticas, veterinárias, alimentares, ambientais, biotecnológicas ou de biossegurança, tendo particular utilidade no diagnóstico rápido, de baixo custo e em ponto de interesse do vírus SARS-CoV-2.INTEGRATED SYSTEM FOR THE DETECTION AND IDENTIFICATION OF SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND METHOD OF USE OF THE INTEGRATED SYSTEM. The present invention relates to a method and portable device (1) for the detection and identification of specific sequences of nucleic acids in different types of sample, through the use of a reverse transcription technique and/or isothermal amplification, using oligonucleotide primers specific to the target region(s) to be detected, with their amplification being carried out indirectly and non-specifically, or directly and specifically, using discriminatory amplification reagents, whose signal, colorimetric or fluorescence, is recorded by the device laptop (1) where the reaction is carried out. This device is controlled via a mobile application (9) which also records the data acquired by the device (1), analyzes them and stores them on a remote cloud server (10), which in turn analyzes the global set of all data in order to allow a global analysis of, for example, evolution of epidemics. In this way, the present invention is useful for the detection and identification of relevant nucleic sequences for different types of diagnosis in different clinical, pharmaceutical, veterinary, food, environmental, biotechnological or biosafety areas, having particular utility in rapid, low-cost diagnosis. and in point of interest of the SARS-CoV-2 virus.

BR112022021086A 2020-04-30 2021-04-28 INTEGRATED SYSTEM FOR THE DETECTION AND IDENTIFICATION OF SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND METHOD OF USE OF THE INTEGRATED SYSTEM BR112022021086A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT11631720 2020-04-30
PCT/IB2021/053530 WO2021220192A2 (en) 2020-04-30 2021-04-28 Method and portable device for detection of nucleic sequences in suspected coronavirus samples

Publications (1)

Publication Number Publication Date
BR112022021086A2 true BR112022021086A2 (en) 2022-12-13

Family

ID=75787159

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022021086A BR112022021086A2 (en) 2020-04-30 2021-04-28 INTEGRATED SYSTEM FOR THE DETECTION AND IDENTIFICATION OF SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND METHOD OF USE OF THE INTEGRATED SYSTEM

Country Status (8)

Country Link
EP (1) EP4142945A2 (en)
JP (1) JP2023524117A (en)
CN (1) CN115836133A (en)
AU (1) AU2021265579A1 (en)
BR (1) BR112022021086A2 (en)
CA (1) CA3175648A1 (en)
IL (1) IL297281A (en)
WO (1) WO2021220192A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES1298031Y (en) * 2022-02-11 2023-06-02 Consorcio De Aguas De Asturias WASTEWATER OR ENVIRONMENTAL ANALYSIS SYSTEM FOR THE DETECTION OF VIRUSES, BACTERIA OR EUKARYOTIC MICROORGANISMS
WO2024047567A1 (en) * 2022-08-31 2024-03-07 VD-ING d.o.o. Method, device and system for detecting microbial or viral infection
US20240120087A1 (en) * 2022-10-07 2024-04-11 1Drop Inc. Method and apparatus for efficiently transmitting nucleic acid amplification result data made by measuring instrument

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US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
DE1020534T1 (en) 1998-11-09 2001-03-01 Eiken Chemical METHOD FOR SYNTHETIZING NUCLEIC ACID
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
WO2004027025A2 (en) 2002-09-20 2004-04-01 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US20110312739A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Single-use test module for pcr amplification of targets and electrochemiluminescent detection of targets
WO2015031351A1 (en) * 2013-08-26 2015-03-05 Diagenetix, Inc. Hardware and mobile software for operation of portable instruments for nucleic acid amplification
ITUB20159332A1 (en) 2015-12-22 2017-06-22 Diasorin S P A Fluorescence detection procedure of loop-mediated isothermal amplification (LAMP) of a target nucleic acid, related oligonucleotides and kits.
US20170276599A1 (en) * 2016-03-24 2017-09-28 The Board Of Regents Of The University Of Texas System Cost effective battery-powered spectrophotometric system

Also Published As

Publication number Publication date
EP4142945A2 (en) 2023-03-08
WO2021220192A2 (en) 2021-11-04
WO2021220192A3 (en) 2021-12-09
CA3175648A1 (en) 2021-11-04
AU2021265579A1 (en) 2022-11-10
JP2023524117A (en) 2023-06-08
WO2021220192A4 (en) 2022-02-03
CN115836133A (en) 2023-03-21
IL297281A (en) 2022-12-01

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