BR112022012813A2 - Métodos para analisar proteínas do capsídeo de aav - Google Patents

Métodos para analisar proteínas do capsídeo de aav

Info

Publication number
BR112022012813A2
BR112022012813A2 BR112022012813A BR112022012813A BR112022012813A2 BR 112022012813 A2 BR112022012813 A2 BR 112022012813A2 BR 112022012813 A BR112022012813 A BR 112022012813A BR 112022012813 A BR112022012813 A BR 112022012813A BR 112022012813 A2 BR112022012813 A2 BR 112022012813A2
Authority
BR
Brazil
Prior art keywords
capsid proteins
aav
methods
vps
analyze
Prior art date
Application number
BR112022012813A
Other languages
English (en)
Inventor
Daud Kunal
Li Ju
Dealwis Uditha
Original Assignee
Sarepta Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sarepta Therapeutics Inc filed Critical Sarepta Therapeutics Inc
Publication of BR112022012813A2 publication Critical patent/BR112022012813A2/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MÉTODOS PARA ANALISAR PROTEÍNAS DO CAPSÍDEO DE AAV. São fornecidos métodos para caracterizar as proteínas do capsídeo VP1, VP2 e VPS em uma partícula de vírus adenoassociado (AAV) usando cromatografia líquida, espectrometria de massa e/ou espectroscopia visível no ultravioleta (UV). Os métodos geralmente incluem as etapas de (a) submeter uma partícula de AAV à cromatografia líquida para desnaturar e, em seguida, separar as proteínas do capsídeo VP1, VP2 e VPS e (b) submeter as proteínas do capsídeo VP1, VP2 e VPS separadas produzidas na etapa (a) a espectrometria UV e de massa para determinar a razão e as massas das proteínas do capsídeo VP1, VP2 e VPS na partícula AAV. Em outro aspecto, a divulgação fornece uma composição de AAV compreendendo uma modificação pós-tradução. A divulgação também fornece métodos para caracterizar a pureza das composições de AAV usando espectrometria de massa de cromatografia líquida.
BR112022012813A 2020-01-03 2020-12-30 Métodos para analisar proteínas do capsídeo de aav BR112022012813A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202062956681P 2020-01-03 2020-01-03
US202063073188P 2020-09-01 2020-09-01
US202063119909P 2020-12-01 2020-12-01
PCT/US2020/067395 WO2021138381A1 (en) 2020-01-03 2020-12-30 Methods for analyzing aav capsid proteins

Publications (1)

Publication Number Publication Date
BR112022012813A2 true BR112022012813A2 (pt) 2022-09-27

Family

ID=74595360

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022012813A BR112022012813A2 (pt) 2020-01-03 2020-12-30 Métodos para analisar proteínas do capsídeo de aav

Country Status (13)

Country Link
US (1) US20230204595A1 (pt)
EP (1) EP4085255A1 (pt)
JP (1) JP2023509122A (pt)
KR (1) KR20220121823A (pt)
CN (1) CN114902051A (pt)
AU (1) AU2020419156A1 (pt)
BR (1) BR112022012813A2 (pt)
CA (2) CA3165911A1 (pt)
CL (1) CL2022001782A1 (pt)
CO (1) CO2022010402A2 (pt)
IL (1) IL294230A (pt)
MX (1) MX2022008003A (pt)
WO (1) WO2021138381A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL309763A (en) * 2021-07-12 2024-02-01 Regeneron Pharma A liquid chromatography assay for the determination of adeno-associated virus to capsid ratio

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2406743A1 (en) 2000-04-28 2001-11-08 The Trustees Of The University Of Pennsylvania Recombinant aav vectors with aav5 capsids and aav5 vectors pseudotyped in heterologous capsids
BR112014025985A2 (pt) * 2012-04-18 2017-07-11 Childrens Hospital Philadelphia composição e métodos para transferência de gene altamente eficaz com o uso de variantes de capsídeo de aav
AU2017312951B2 (en) 2016-08-15 2024-02-08 Genzyme Corporation Methods for detecting AAV
EP3768695A4 (en) * 2018-02-27 2022-04-06 The Trustees of the University of Pennsylvania NOVEL ADENO-ASSOCIATED VIRUS (AAV) VECTORS, AAV VECTORS WITH REDUCED CAPSIID DEAMIDATION AND USES THEREOF
CA3091806A1 (en) * 2018-02-27 2019-09-06 The Trustees Of The University Of Pennsylvania Novel adeno-associated virus (aav) vectors, aav vectors having reduced capsid deamidation and uses therefor

Also Published As

Publication number Publication date
EP4085255A1 (en) 2022-11-09
KR20220121823A (ko) 2022-09-01
CN114902051A (zh) 2022-08-12
US20230204595A1 (en) 2023-06-29
AU2020419156A1 (en) 2022-08-18
CA3222852A1 (en) 2021-07-08
CL2022001782A1 (es) 2023-02-03
CA3165911A1 (en) 2021-07-08
WO2021138381A1 (en) 2021-07-08
MX2022008003A (es) 2022-10-07
CO2022010402A2 (es) 2022-10-31
IL294230A (en) 2022-08-01
JP2023509122A (ja) 2023-03-07

Similar Documents

Publication Publication Date Title
Hendy et al. A guide to ancient protein studies
CO2020004738A2 (es) Variantes de cápsides de virus adenoasociados y métodos de uso de estas
Plum et al. Combined enrichment of neuromelanin granules and synaptosomes from human substantia nigra pars compacta tissue for proteomic analysis
EA201792236A1 (ru) Получение увеличенных в размере векторов на основе аденоассоциированного вируса
BR112019001532A2 (pt) proteínas de capsídeo de vírus adenoassociado inovadoras
BR112021018776A2 (pt) Vetores de vírus adenoassociado recombinante
BR112018071180A2 (pt) métodos de reforço potência biológica de baculovirus system-produzido vírus adeno-associado recombinante
BR112022012813A2 (pt) Métodos para analisar proteínas do capsídeo de aav
BR112017017028A2 (pt) rnai variante
PL404537A1 (pl) Wirusy stowarzyszone z adenowirusem, kompozycje, wyizolowane bialko kapsydu, wyizolowana lub syntetyczna czasteczka kwasu nukleinowego, sposób wytwarzania rekombinowanych wirusów, komórki gospodarza, bialka obejmujace fragment bialka kapsydu AAV, sztuczne bialka, rekombinowane wirusy, czasteczki, sposoby dostarczania transgenu do komórki, sposób identyfikacji serotypu sekwencji wirusa (AAV), zestaw diagnostyczny, sposób izolacji nowych wirusów, nowe serotypy wirusa, wyizolowane wirusy, rekombinowana komórka, zastosowanie wirusa
BR112018071686A2 (pt) exibição de proteínas integrais de membrana em vírions extracelulares envelopados de poxvírus
Zhang et al. Quantitative chemical proteomics for investigating the biomarkers of dioscin against liver fibrosis caused by CCl 4 in rats
US20240102046A9 (en) Methods for analysis of viral capsid protein composition
WO2019067982A3 (en) Production of adeno-associated viruses in insect cells
Oldrati et al. Peptidomic and transcriptomic profiling of four distinct spider venoms
BR112015026153A2 (pt) correção de perda de quadro por injeção de ruído ponderado
BR112021020706A2 (pt) Métodos e composições para expressão de transgene
BR112023024375A2 (pt) Vírus adeno-associado recombinante tendo capsídeo variante e sua aplicação
Xiong et al. Chloroform-assisted phenol extraction improving proteome profiling of maize embryos through selective depletion of high-abundance storage proteins
Xie et al. A novel method of protein extraction from perennial Bupleurum root for 2‐DE
AR120984A1 (es) Métodos para analizar proteínas de la cápside de aav
Sun et al. Protein extraction from the stem of Panax ginseng CA Meyer: a tissue of lower protein extraction efficiency for proteomic analysis
Faça et al. The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)
Chen et al. Integrated immunopeptidomics and proteomics study reveals imbalanced innate and adaptive immune responses to SARS-CoV-2 infection
Mouchbahani-Constance et al. Proteomic and Transcriptomic Techniques to Decipher the Molecular Evolution of Venoms. Toxins 2021, 13, 154