BR112022012602A2 - Método de construção de fungos geneticamente modificados de fungos filamentosos e método de produção de etanol - Google Patents

Método de construção de fungos geneticamente modificados de fungos filamentosos e método de produção de etanol

Info

Publication number
BR112022012602A2
BR112022012602A2 BR112022012602A BR112022012602A BR112022012602A2 BR 112022012602 A2 BR112022012602 A2 BR 112022012602A2 BR 112022012602 A BR112022012602 A BR 112022012602A BR 112022012602 A BR112022012602 A BR 112022012602A BR 112022012602 A2 BR112022012602 A2 BR 112022012602A2
Authority
BR
Brazil
Prior art keywords
fungi
ethanol
filamentous
dehydrogenase
overexpression
Prior art date
Application number
BR112022012602A
Other languages
English (en)
Inventor
Tian Chaoguang
Zhang Yongli
Li Jingen
Li Jinyang
Sun Tao
Liu Qian
Sun Wenliang
Original Assignee
Tianjin Inst Ind Biotechnology Cas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201911349044.4A external-priority patent/CN113025506B/zh
Priority claimed from CN202010874879.8A external-priority patent/CN114107357B/zh
Priority claimed from CN202011283377.4A external-priority patent/CN114507608B/zh
Application filed by Tianjin Inst Ind Biotechnology Cas filed Critical Tianjin Inst Ind Biotechnology Cas
Publication of BR112022012602A2 publication Critical patent/BR112022012602A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MÉTODO DE CONSTRUÇÃO DE FUNGOS GENETICAMENTE MODIFICADOS DE FUNGOS FILAMENTOSOS E MÉTODO DE PRODUÇÃO DE ETANOL. A invenção revela um método de construção de fungos geneticamente modificados de fungos filamentosos. O método compreende: permitir, por um método de engenharia genética, o fungo filamentoso superexpressar um gene de regulação positiva da síntese de etanol, e/ou regular para baixo o gene de regulação negativa da síntese de etanol endógeno; ou em uma célula fúngica filamentosa, a superexpressão da acetaldeído desidrogenase e etanol desidrogenase contendo sequência de sinais da localização mitocondrial, ou a superexpressão da piruvato descarboxilase e etanol desidrogenase contendo sequência de sinais da localização mitocondrial, a ou superexpressão de acetaldeído desidrogenase, etanol desidrogenase e piruvato descarboxilase contendo sequência de sinais da localização mitocondrial. Em comparação com a cepa original, a capacidade de síntese de etanol das cepas obtidas geneticamente é melhorada.
BR112022012602A 2019-12-24 2020-12-09 Método de construção de fungos geneticamente modificados de fungos filamentosos e método de produção de etanol BR112022012602A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201911349044.4A CN113025506B (zh) 2019-12-24 2019-12-24 一种生产乙醇的重组丝状真菌及其构建和应用
CN202010874879.8A CN114107357B (zh) 2020-08-27 2020-08-27 一种磷酸果糖激酶2突变体生产乙醇的重组丝状真菌及其构建和产乙醇的应用
CN202011283377.4A CN114507608B (zh) 2020-11-17 2020-11-17 一种在线粒体内生产乙醇的重组丝状真菌及其构建和应用
PCT/CN2020/134922 WO2021129396A1 (zh) 2019-12-24 2020-12-09 一种生产乙醇的重组丝状真菌及其构建和应用

Publications (1)

Publication Number Publication Date
BR112022012602A2 true BR112022012602A2 (pt) 2022-12-13

Family

ID=76573721

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022012602A BR112022012602A2 (pt) 2019-12-24 2020-12-09 Método de construção de fungos geneticamente modificados de fungos filamentosos e método de produção de etanol

Country Status (4)

Country Link
US (1) US20230053729A1 (pt)
EP (1) EP4063484A4 (pt)
BR (1) BR112022012602A2 (pt)
WO (1) WO2021129396A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4353825A1 (en) * 2022-10-10 2024-04-17 bisy GmbH Modified promoter sequences

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106148209B (zh) * 2015-03-23 2020-11-20 中国科学院天津工业生物技术研究所 新的二元有机酸生产菌株及其制备和应用
CN107236757A (zh) * 2016-03-28 2017-10-10 中国科学院天津工业生物技术研究所 一种提高丝状真菌木质纤维素酶系表达及生物基化学品生产的方法

Also Published As

Publication number Publication date
WO2021129396A1 (zh) 2021-07-01
EP4063484A4 (en) 2023-05-03
US20230053729A1 (en) 2023-02-23
EP4063484A1 (en) 2022-09-28

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