BR112021024597A2 - Células geneticamente engenheiradas sensíveis para neurotoxinas clostridiais - Google Patents

Células geneticamente engenheiradas sensíveis para neurotoxinas clostridiais

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Publication number
BR112021024597A2
BR112021024597A2 BR112021024597A BR112021024597A BR112021024597A2 BR 112021024597 A2 BR112021024597 A2 BR 112021024597A2 BR 112021024597 A BR112021024597 A BR 112021024597A BR 112021024597 A BR112021024597 A BR 112021024597A BR 112021024597 A2 BR112021024597 A2 BR 112021024597A2
Authority
BR
Brazil
Prior art keywords
genetically engineered
engineered cells
cells sensitive
clostridial neurotoxins
clostridial
Prior art date
Application number
BR112021024597A
Other languages
English (en)
Inventor
Barry Gertz
A Oyler George
Original Assignee
Synaptic Res Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synaptic Res Llc filed Critical Synaptic Res Llc
Publication of BR112021024597A2 publication Critical patent/BR112021024597A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

células geneticamente engenheiradas sensíveis para neurotoxinas clostridiais. uma célula que foi geneticamente engenheirada para ser altamente sensitiva à neurotoxina clostridial, por exemplo, neurotoxina botulínica e neurotoxina do tétano ou versões modificadas ou recombinantes das mesmas. um método para fabricar uma tal célula geneticamente engenheirada e um método para usar uma tal célula no ensaio da atividade de neurotoxina clostridial modificada ou recombinante.
BR112021024597A 2019-06-07 2020-06-08 Células geneticamente engenheiradas sensíveis para neurotoxinas clostridiais BR112021024597A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962858384P 2019-06-07 2019-06-07
PCT/IB2020/055377 WO2020245810A1 (en) 2019-06-07 2020-06-08 Genetically engineered cells sensitive for clostridial neurotoxins

Publications (1)

Publication Number Publication Date
BR112021024597A2 true BR112021024597A2 (pt) 2022-02-15

Family

ID=71738185

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021024597A BR112021024597A2 (pt) 2019-06-07 2020-06-08 Células geneticamente engenheiradas sensíveis para neurotoxinas clostridiais

Country Status (11)

Country Link
US (1) US20220298488A1 (pt)
EP (1) EP3980532A1 (pt)
JP (1) JP2022543521A (pt)
KR (1) KR20220050872A (pt)
CN (1) CN114555787A (pt)
AR (1) AR119109A1 (pt)
AU (1) AU2020287269A1 (pt)
BR (1) BR112021024597A2 (pt)
CA (1) CA3142567A1 (pt)
TW (1) TW202113072A (pt)
WO (1) WO2020245810A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021028868A1 (en) * 2019-08-13 2021-02-18 Synaptic Research, Llc Cells highly sensitive to clostridial neurotoxin

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
WO2006107921A2 (en) * 2005-04-05 2006-10-12 Allergan, Inc. Lipophilic dye-based fret assays for clostridial toxin activity
JP5412689B2 (ja) * 2007-09-14 2014-02-12 バイオマデイソン・インコーポレイテツド 切断配列とスペーサーを用いた共鳴エネルギー転移アッセイ
CA2783242A1 (en) * 2009-12-07 2011-06-16 Synaptic Research, Llc Method for identification of protease activity inhibitors and assaying the presence of protease activity
CA2801421A1 (en) 2010-06-11 2012-04-12 Synaptic Research, Llc N-end rule protease activity indication methods and uses thereof
EP3030905B9 (en) * 2013-08-09 2024-04-03 Biomadison, Inc. Botulinum toxin assay with improved sensitivity

Also Published As

Publication number Publication date
WO2020245810A1 (en) 2020-12-10
AU2020287269A1 (en) 2022-01-06
CA3142567A1 (en) 2020-12-10
KR20220050872A (ko) 2022-04-25
AR119109A1 (es) 2021-11-24
CN114555787A (zh) 2022-05-27
US20220298488A1 (en) 2022-09-22
EP3980532A1 (en) 2022-04-13
TW202113072A (zh) 2021-04-01
JP2022543521A (ja) 2022-10-13

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