BR112021023648A2 - Ferramenta genética otimizada para modificar as bactérias - Google Patents

Ferramenta genética otimizada para modificar as bactérias

Info

Publication number
BR112021023648A2
BR112021023648A2 BR112021023648A BR112021023648A BR112021023648A2 BR 112021023648 A2 BR112021023648 A2 BR 112021023648A2 BR 112021023648 A BR112021023648 A BR 112021023648A BR 112021023648 A BR112021023648 A BR 112021023648A BR 112021023648 A2 BR112021023648 A2 BR 112021023648A2
Authority
BR
Brazil
Prior art keywords
bacterium
sequence
genetic
bacteria
modification
Prior art date
Application number
BR112021023648A
Other languages
English (en)
Inventor
François WASELS
Lopes Ferreira Nicolas
Rémi Hocq
Original Assignee
Ifp Energies Now
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ifp Energies Now filed Critical Ifp Energies Now
Publication of BR112021023648A2 publication Critical patent/BR112021023648A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

ferramenta genética otimizada para modificar as bactérias. a presente invenção se refere à transformação e à modificação genética de bactérias pertencentes ao filo das firmicutes. a mesma se refere ainda a métodos, ferramentas e kits que permitem uma tal modificação genética, que implica em particular em uma sequência de ácido nucleico utilizada para facilitar a transformação da bactéria, em que a dita sequência compreende i) toda ou parte da sequência seq id no: 126 e ii) uma sequência que permite a modificação do material genético de uma bactéria e/ou a expressão no seio da dita bactéria de uma sequência de dna parcialmente ou totalmente ausente do material genético presente no seio da versão selvagem da dita bactéria. a descrição visa também as bactérias geneticamente modificadas obtidas e suas utilizações, em particular para produzir um solvente, de preferência na escala industrial.
BR112021023648A 2019-05-24 2020-05-22 Ferramenta genética otimizada para modificar as bactérias BR112021023648A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1905485A FR3096373A1 (fr) 2019-05-24 2019-05-24 Outil genetique optimisé pour modifier les bacteries
PCT/FR2020/050853 WO2020240122A1 (fr) 2019-05-24 2020-05-22 Outil genetique optimisé pour modifier les bacteries

Publications (1)

Publication Number Publication Date
BR112021023648A2 true BR112021023648A2 (pt) 2022-01-04

Family

ID=69157897

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021023648A BR112021023648A2 (pt) 2019-05-24 2020-05-22 Ferramenta genética otimizada para modificar as bactérias

Country Status (9)

Country Link
US (1) US20220243170A1 (pt)
EP (1) EP3976780A1 (pt)
JP (1) JP2022534223A (pt)
KR (1) KR20220012324A (pt)
CN (1) CN114286857A (pt)
BR (1) BR112021023648A2 (pt)
CA (1) CA3141382A1 (pt)
FR (1) FR3096373A1 (pt)
WO (1) WO2020240122A1 (pt)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6905518B2 (ja) * 2015-10-13 2021-07-21 ランザテク・ニュージーランド・リミテッド エネルギー発生発酵経路を含む遺伝子操作細菌
FR3042506B1 (fr) 2015-10-16 2018-11-30 IFP Energies Nouvelles Outil genetique de transformation de bacteries clostridium
FR3081881B1 (fr) * 2018-06-04 2024-05-24 Ifp Energies Now Outil genetique optimise pour modifier les bacteries du genre clostridium
FR3090691B1 (fr) * 2018-12-20 2023-06-09 Ifp Energies Now Bacteries clostridium genetiquement modifiees, preparation et utilisations de celles-ci

Also Published As

Publication number Publication date
FR3096373A1 (fr) 2020-11-27
KR20220012324A (ko) 2022-02-03
CN114286857A (zh) 2022-04-05
WO2020240122A1 (fr) 2020-12-03
JP2022534223A (ja) 2022-07-28
US20220243170A1 (en) 2022-08-04
CA3141382A1 (fr) 2020-12-03
EP3976780A1 (fr) 2022-04-06

Similar Documents

Publication Publication Date Title
PH12019501344A1 (en) Thermostable cas9 nucleases
PH12017502281A1 (en) Thermostable cas9 nucleases
CL2021002741A1 (es) Una proteína crispr de prevotella y francisella 1 (cpf1) aislada de lachnospiraceae bacterium nd2006; proteína de fusión que la comprende; ácido nucleico; vector; métodos para alterar el genoma de una célula o una molécula de adn bicatenario in vitro que utilizan dichas proteínas (divisional de sol. 201903004)
BR112016018287A2 (pt) proteínas inseticidas e métodos para uso das mesmas
BR112017007932A2 (pt) proteínas inseticidas e métodos para uso das mesmas
Srikulnath et al. Identification of the linkage group of the Z sex chromosomes of the sand lizard (Lacerta agilis, Lacertidae) and elucidation of karyotype evolution in lacertid lizards
BR122023016256B8 (pt) Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo
AR103411A1 (es) Proteínas insecticidas y métodos para su uso
BR112016003225A2 (pt) proteínas inseticidas e métodos para uso das mesmas
AR057205A1 (es) Genes grg23 y grg51 que confieren resistencia a herbicidas
DK1131450T3 (da) Kimære promotorer, der kan formidle genekspression i planter ved patogeninfektion, og anvendelser heraf
PH12019500583A1 (en) Novel paratransgenic system for the biocontrol of disease-transmitting mosquitos
Carvalho et al. Reference genes for RT-qPCR studies in Corynebacterium pseudotuberculosis identified through analysis of RNA-seq data
BRPI0512791A (pt) sistema de transferência de gene biológico para células eucarióticas
BR112017019194A2 (pt) métodos com base em estrutura para modificação de polipeptídeos pip-72 e polipeptídeos pip-72 derivados da mesma
AR110792A1 (es) Proteínas insecticidas y métodos para su uso
BR112021023648A2 (pt) Ferramenta genética otimizada para modificar as bactérias
WO2019204643A3 (en) Animal pathogen-derived polypeptides and uses thereof for genetic engineering
MX2018015720A (es) Planta transgenica que tiene resistencia a herbicidas.
BR112018071457A2 (pt) evento elite ee-gh7 e métodos e kits para identificação desse evento em amostras biológicas
BR112017019419A2 (pt) combinações inseticidas de pip-72 e métodos de uso
Cai et al. Isolation and characterization of microsatellite markers from Lagerstroemia caudata (Lythraceae) and cross-amplification in other related species
CR6905A (es) Bacterias insecticidas mejoradas y metodos para hacerlas y usarlas.
PH12018501814A1 (en) Insecticidal polypeptides having improved activity spectrum and uses thereof
AR070146A1 (es) Gen de bacillus thuringiensis (bt) con actividad contra coleopteros