BR112021016019A2 - Sistemas e proteínas de fusão crispr/cas - Google Patents
Sistemas e proteínas de fusão crispr/cas Download PDFInfo
- Publication number
- BR112021016019A2 BR112021016019A2 BR112021016019-1A BR112021016019A BR112021016019A2 BR 112021016019 A2 BR112021016019 A2 BR 112021016019A2 BR 112021016019 A BR112021016019 A BR 112021016019A BR 112021016019 A2 BR112021016019 A2 BR 112021016019A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- protein
- sequence
- fusion protein
- genetically modified
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/205—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Campylobacter (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962806708P | 2019-02-15 | 2019-02-15 | |
| US62/806,708 | 2019-02-15 | ||
| PCT/US2020/018145 WO2020168102A1 (en) | 2019-02-15 | 2020-02-13 | Crispr/cas fusion proteins and systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021016019A2 true BR112021016019A2 (pt) | 2021-10-05 |
Family
ID=70166116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021016019-1A BR112021016019A2 (pt) | 2019-02-15 | 2020-02-13 | Sistemas e proteínas de fusão crispr/cas |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US10947517B2 (https=) |
| EP (1) | EP3924475A1 (https=) |
| JP (2) | JP2022520104A (https=) |
| KR (1) | KR20210139271A (https=) |
| CN (1) | CN113728099A (https=) |
| AU (2) | AU2020221274B2 (https=) |
| BR (1) | BR112021016019A2 (https=) |
| CA (1) | CA3129835A1 (https=) |
| IL (1) | IL285300A (https=) |
| SG (1) | SG11202108812SA (https=) |
| WO (1) | WO2020168102A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| CA3129835A1 (en) | 2019-02-15 | 2020-08-20 | Sigma-Aldrich Co. Llc | Crispr/cas fusion proteins and systems |
| JP2024533311A (ja) * | 2021-09-08 | 2024-09-12 | フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー | ゲノムを調節するための方法及び組成物 |
| CN116063546B (zh) * | 2021-10-29 | 2026-01-16 | 中科辅酶医科技(深圳)有限公司 | 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用 |
| US20230265405A1 (en) * | 2022-02-22 | 2023-08-24 | Massachusetts Institute Of Technology | Engineered nucleases and methods of use thereof |
| JP2025510574A (ja) * | 2022-03-08 | 2025-04-15 | ウェイク・フォレスト・ユニバーシティ・ヘルス・サイエンシーズ | 真核生物遺伝子編集のための組成物、システムおよび方法 |
| WO2025221793A1 (en) * | 2024-04-16 | 2025-10-23 | Monsanto Technology Llc | Modified cas proteins for nuclear localization and editing activity in plants |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030232430A1 (en) | 2001-11-26 | 2003-12-18 | Advanced Cell Technology | Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| EP3363902B1 (en) * | 2012-12-06 | 2019-11-27 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| WO2014186686A2 (en) * | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
| CA2915296A1 (en) * | 2013-06-11 | 2015-02-26 | Portage Pharmaceuticals Ltd. | Structure, manufacturing and uses of human-derived cell-permeable peptides conjugated with specific biologically active cargo peptides |
| JP6793547B2 (ja) * | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物 |
| DK3107562T3 (da) | 2014-02-19 | 2019-12-16 | Bioasis Technologies Inc | P97-ids-fusionsproteiner |
| US11242525B2 (en) | 2014-03-26 | 2022-02-08 | Editas Medicine, Inc. | CRISPR/CAS-related methods and compositions for treating sickle cell disease |
| WO2016054326A1 (en) | 2014-10-01 | 2016-04-07 | The General Hospital Corporation | Methods for increasing efficiency of nuclease-induced homology-directed repair |
| WO2016210271A1 (en) | 2015-06-24 | 2016-12-29 | Sigma-Aldrich Co. Llc | Cell cycle dependent genome regulation and modification |
| JP6867689B2 (ja) * | 2016-01-29 | 2021-05-12 | 国立大学法人東京工業大学 | Ｃａｓ９−ｇＲＮＡ複合体の細胞核内への導入方法、及び細胞内における標的遺伝子の改変方法 |
| WO2017173004A1 (en) * | 2016-03-30 | 2017-10-05 | Mikuni Takayasu | A method for in vivo precise genome editing |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| CN107579793A (zh) * | 2016-07-04 | 2018-01-12 | 中兴通讯股份有限公司 | 一种通信网络设备间时间同步的优化方法、装置及设备 |
| KR102547316B1 (ko) * | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| EP3526320A1 (en) * | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| JP7182545B2 (ja) | 2016-12-14 | 2022-12-02 | ヴァーヘニンゲン ユニヴェルシテット | 熱安定性ｃａｓ９ヌクレアーゼ |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| GB201710973D0 (en) | 2017-07-07 | 2017-08-23 | Avacta Life Sciences Ltd | Scaffold proteins |
| KR20200036912A (ko) | 2017-07-31 | 2020-04-07 | 리플렉션 바이오테크놀러지스 리미티드 | 안과 질환을 위한 세포 모델 및 치료요법 |
| US11071791B2 (en) * | 2018-01-26 | 2021-07-27 | Wisconsin Alumni Research Foundation | Vector for gene silencing and replacement and methods of use thereof |
| CN111902536B (zh) | 2018-02-15 | 2024-07-30 | 西格马-奥尔德里奇有限责任公司 | 用于真核基因组修饰的改造的cas9系统 |
| PL3765615T3 (pl) * | 2018-03-14 | 2023-11-13 | Arbor Biotechnologies, Inc. | Nowe enzymy i układy crispr ukierunkowane na dna |
| CA3129835A1 (en) | 2019-02-15 | 2020-08-20 | Sigma-Aldrich Co. Llc | Crispr/cas fusion proteins and systems |
-
2020
- 2020-02-13 CA CA3129835A patent/CA3129835A1/en active Pending
- 2020-02-13 BR BR112021016019-1A patent/BR112021016019A2/pt not_active IP Right Cessation
- 2020-02-13 SG SG11202108812SA patent/SG11202108812SA/en unknown
- 2020-02-13 WO PCT/US2020/018145 patent/WO2020168102A1/en not_active Ceased
- 2020-02-13 EP EP20716979.8A patent/EP3924475A1/en not_active Withdrawn
- 2020-02-13 AU AU2020221274A patent/AU2020221274B2/en not_active Ceased
- 2020-02-13 JP JP2021547390A patent/JP2022520104A/ja active Pending
- 2020-02-13 US US16/790,399 patent/US10947517B2/en active Active
- 2020-02-13 KR KR1020217029732A patent/KR20210139271A/ko not_active Ceased
- 2020-02-13 CN CN202080029346.8A patent/CN113728099A/zh active Pending
-
2021
- 2021-02-10 US US17/172,734 patent/US11965184B2/en active Active
- 2021-08-02 IL IL285300A patent/IL285300A/en unknown
-
2023
- 2023-04-27 US US18/308,336 patent/US20230287377A1/en not_active Abandoned
- 2023-07-27 JP JP2023122299A patent/JP2023156365A/ja active Pending
-
2024
- 2024-04-09 AU AU2024202275A patent/AU2024202275A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP3924475A1 (en) | 2021-12-22 |
| CA3129835A1 (en) | 2020-08-20 |
| KR20210139271A (ko) | 2021-11-22 |
| WO2020168102A1 (en) | 2020-08-20 |
| IL285300A (en) | 2021-09-30 |
| AU2020221274A1 (en) | 2021-08-26 |
| SG11202108812SA (en) | 2021-09-29 |
| US11965184B2 (en) | 2024-04-23 |
| CN113728099A (zh) | 2021-11-30 |
| AU2020221274B2 (en) | 2024-02-08 |
| US10947517B2 (en) | 2021-03-16 |
| JP2022520104A (ja) | 2022-03-28 |
| US20200263155A1 (en) | 2020-08-20 |
| JP2023156365A (ja) | 2023-10-24 |
| AU2024202275A1 (en) | 2024-05-23 |
| US20230287377A1 (en) | 2023-09-14 |
| US20210163910A1 (en) | 2021-06-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2866 DE 09/12/2025. |