BR112019012414A2 - Carregamento de moléculas em poços de amostra para análise - Google Patents

Carregamento de moléculas em poços de amostra para análise Download PDF

Info

Publication number
BR112019012414A2
BR112019012414A2 BR112019012414-4A BR112019012414A BR112019012414A2 BR 112019012414 A2 BR112019012414 A2 BR 112019012414A2 BR 112019012414 A BR112019012414 A BR 112019012414A BR 112019012414 A2 BR112019012414 A2 BR 112019012414A2
Authority
BR
Brazil
Prior art keywords
sample
weight
molecule
agent
interest
Prior art date
Application number
BR112019012414-4A
Other languages
English (en)
Portuguese (pt)
Inventor
M. Rothberg Jonathan
A. Ball James
Lackey Jeremy
Reed Brian
Goryaynov Alexander
Original Assignee
Quantum-Si Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112019012414A2 publication Critical patent/BR112019012414A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Rigid containers without fluid transport within
    • B01L3/5085Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50857Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices
    • B01L9/52Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L1/00Compositions of cellulose, modified cellulose or cellulose derivatives
    • C08L1/02Cellulose; Modified cellulose
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L71/00Compositions of polyethers obtained by reactions forming an ether link in the main chain; Compositions of derivatives of such polymers
    • C08L71/08Polyethers derived from hydroxy compounds or from their metallic derivatives
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0642Filling fluids into wells by specific techniques
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0829Multi-well plates; Microtitration plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0893Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2203/00Applications
    • C08L2203/16Applications used for films
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/648Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
BR112019012414-4A 2016-12-19 2017-12-19 Carregamento de moléculas em poços de amostra para análise BR112019012414A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662436407P 2016-12-19 2016-12-19
US62/436,407 2016-12-19
PCT/US2017/067421 WO2018118992A1 (en) 2016-12-19 2017-12-19 Loading molecules into sample wells for analysis

Publications (1)

Publication Number Publication Date
BR112019012414A2 true BR112019012414A2 (pt) 2020-02-27

Family

ID=61132890

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019012414-4A BR112019012414A2 (pt) 2016-12-19 2017-12-19 Carregamento de moléculas em poços de amostra para análise

Country Status (11)

Country Link
US (1) US10731209B2 (https=)
EP (1) EP3555314B1 (https=)
JP (1) JP7167024B2 (https=)
KR (1) KR102591300B1 (https=)
CN (1) CN110088293A (https=)
AU (1) AU2017378985A1 (https=)
BR (1) BR112019012414A2 (https=)
CA (1) CA3046680A1 (https=)
MX (1) MX2019007247A (https=)
TW (1) TW201837184A (https=)
WO (1) WO2018118992A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3377656B1 (en) 2015-11-18 2025-07-02 Pacific Biosciences of California, Inc. Loading nucleic acids onto substrates
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
CN111939844A (zh) * 2020-09-10 2020-11-17 中国人民解放军军事科学院军事医学研究院 一种dna合成微孔板
US20220098658A1 (en) * 2020-09-21 2022-03-31 Quantum-Si Incorporated Methods to minimize photodamage during nucleic acid and peptide sequencing
JP2023547110A (ja) 2020-10-22 2023-11-09 クアンタム-エスアイ インコーポレイテッド 順次結合電荷蓄積部を備える集積回路および関連技術
JP2023554368A (ja) 2020-12-15 2023-12-27 クアンタム-エスアイ インコーポレイテッド 単一分子速度論による分子バーコード分析
WO2022246723A1 (zh) * 2021-05-27 2022-12-01 京东方科技集团股份有限公司 检测芯片及其制备方法和进样方法
KR20240033032A (ko) * 2021-07-09 2024-03-12 세페이드 높은 수준의 다중화 반응 용기, 시약 점적 장치 및 관련 방법

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
AU4690596A (en) 1994-12-30 1996-07-24 Chiron Viagene, Inc. Nucleic acid condensing agents with reduced immunogenicity
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
ATE556149T1 (de) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US6429258B1 (en) 1999-05-20 2002-08-06 E. I. Du Pont De Nemours & Company Polymerization of fluoromonomers
US6517776B1 (en) * 2000-11-03 2003-02-11 Chevron Phillips Chemical Company Lp UV oxygen scavenging initiation in angular preformed packaging articles
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
JP2007532116A (ja) 2004-04-07 2007-11-15 キアジェン ノース アメリカン ホールディングス,インコーポレイティド 1成分型および2成分型のDNAPolIIIレプリカーゼとその使用
US7288514B2 (en) 2005-04-14 2007-10-30 The Clorox Company Polymer-fluorosurfactant associative complexes
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
CN101495654A (zh) 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
JP6001243B2 (ja) * 2011-08-11 2016-10-05 株式会社ブリヂストン 衝撃吸収体、及び衝撃吸収体の製造方法
AU2013249123A1 (en) 2012-04-19 2014-12-11 Life Technologies Corporation Method of performing digital PCR
US9797011B2 (en) * 2013-09-13 2017-10-24 Life Technologies Corporation Device preparation using condensed nucleic acid particles
WO2015074001A1 (en) 2013-11-17 2015-05-21 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9587263B2 (en) 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3471402B1 (en) 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrated device for temporal binning of received photons
EP4421188A3 (en) 2014-08-08 2024-10-23 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
CA2957543A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
CN106574266A (zh) * 2014-08-14 2017-04-19 雅培分子公司 用于下一代测序的文库生成
US10605730B2 (en) 2015-05-20 2020-03-31 Quantum-Si Incorporated Optical sources for fluorescent lifetime analysis
US10246742B2 (en) 2015-05-20 2019-04-02 Quantum-Si Incorporated Pulsed laser and bioanalytic system
EP3377656B1 (en) * 2015-11-18 2025-07-02 Pacific Biosciences of California, Inc. Loading nucleic acids onto substrates
EP3458465B1 (en) 2016-05-20 2024-05-08 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
EP4113104A3 (en) 2016-06-01 2023-02-22 Quantum-si Incorporated Integrated device for detecting and analyzing molecules
TWI755400B (zh) 2016-06-01 2022-02-21 美商寬騰矽公司 脈衝命名器及基質命名器、識別核苷酸之方法、校準定序儀器之方法、識別發生核苷酸併入事件之時間的方法、非暫時性電腦可讀儲存媒體、及定序儀器
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재

Also Published As

Publication number Publication date
WO2018118992A1 (en) 2018-06-28
TW201837184A (zh) 2018-10-16
CA3046680A1 (en) 2018-06-28
KR102591300B1 (ko) 2023-10-20
JP7167024B2 (ja) 2022-11-08
US20180223353A1 (en) 2018-08-09
EP3555314A1 (en) 2019-10-23
AU2017378985A1 (en) 2019-06-20
MX2019007247A (es) 2019-08-16
US10731209B2 (en) 2020-08-04
CN110088293A (zh) 2019-08-02
JP2020513749A (ja) 2020-05-21
KR20190098184A (ko) 2019-08-21
EP3555314B1 (en) 2023-08-16

Similar Documents

Publication Publication Date Title
BR112019012414A2 (pt) Carregamento de moléculas em poços de amostra para análise
JP2022174137A (ja) 化学組成物とそれを利用する方法
JP7542000B2 (ja) 核酸分析用の多価性結合組成物
EP2875131B1 (en) A method of normalizing biological samples
ES2990227T3 (es) Composiciones y procedimientos de análisis de ácido nucleico de secuencia única
CN116323974A (zh) 多路复用covid-19锁式测定
JP7332235B2 (ja) ポリヌクレオチドを配列決定する方法
US20110287432A1 (en) System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
CN112567048A (zh) 三元复合物种类的连续形成
EP3988670B1 (en) Single-channel sequencing method based on self-luminescence
EP2817413A1 (en) System and method for generation and use of compact clonally amplified products
JP2018518947A (ja) ヌクレオチドのオルトゴナルな脱ブロッキング
KR20230035237A (ko) 재조합 말단 데옥시뉴클레오티딜 트랜스퍼라제를 사용하여 변형된 염기를 갖는 핵산의 생성
ES2716094T3 (es) Métodos para analizar la contaminación en la secuenciación del ADN
EP2840148B1 (en) Methods for nucleic acid amplification
HK40013623B (en) Loading molecules into sample wells for analysis
HK40013623A (en) Loading molecules into sample wells for analysis
US20230340592A1 (en) Targeted sequencing
US20260015601A1 (en) Improved loading of molecules and complexes to reaction sites
WO2014070540A1 (en) Sequencing-based quantification of nucleic acid targets
CN115836135A (zh) 用于修饰聚合酶-核酸复合物的组合物和方法
HK40067679A (en) Multivalent binding composition for nucleic acid analysis
HK40049621B (zh) 对多核苷酸进行测序的方法

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements