BR112018015831A2 - promotores contendo íntron e uso dos mesmos - Google Patents

promotores contendo íntron e uso dos mesmos

Info

Publication number
BR112018015831A2
BR112018015831A2 BR112018015831A BR112018015831A BR112018015831A2 BR 112018015831 A2 BR112018015831 A2 BR 112018015831A2 BR 112018015831 A BR112018015831 A BR 112018015831A BR 112018015831 A BR112018015831 A BR 112018015831A BR 112018015831 A2 BR112018015831 A2 BR 112018015831A2
Authority
BR
Brazil
Prior art keywords
promoters
intron
rhodosporidium
fungi
yeast
Prior art date
Application number
BR112018015831A
Other languages
English (en)
Inventor
Mei John Koh Chong
Ji Lianghui
Amy Yap Sihui
Liu Yanbin
Original Assignee
Temasek Life Sciences Laboratory Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Temasek Life Sciences Laboratory Ltd filed Critical Temasek Life Sciences Laboratory Ltd
Publication of BR112018015831A2 publication Critical patent/BR112018015831A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se ao campo da biologia molecular e mais particularmente aos promotores úteis para o planejamento metabólico em leveduras ou fungos para a produção de produtos químicos de base biológica com amplas aplicações. os promotores contendo íntrons com forte atividade durante os estágios de acúmulo de óleo são particularmente úteis para a engenharia genética em leveduras e fungos, particularmente os gêneros rhodosporidium ou rhodotorula. tais promotores são capazes de conduzir uma forte expressão de rna ou proteínas em espécies dos gêneros rhodosporidium ou rhodotorula.
BR112018015831A 2016-02-05 2017-02-02 promotores contendo íntron e uso dos mesmos BR112018015831A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662292030P 2016-02-05 2016-02-05
PCT/SG2017/050046 WO2017135895A1 (en) 2016-02-05 2017-02-02 Intron-containing promoters and uses thereof

Publications (1)

Publication Number Publication Date
BR112018015831A2 true BR112018015831A2 (pt) 2018-12-26

Family

ID=59500854

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018015831A BR112018015831A2 (pt) 2016-02-05 2017-02-02 promotores contendo íntron e uso dos mesmos

Country Status (6)

Country Link
US (1) US11254943B2 (pt)
EP (1) EP3411485B1 (pt)
CN (1) CN109072247B (pt)
BR (1) BR112018015831A2 (pt)
DK (1) DK3411485T3 (pt)
WO (1) WO2017135895A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112980709B (zh) * 2019-12-13 2022-06-03 中国科学院大连化学物理研究所 一种工程菌株及其构建方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7459546B2 (en) * 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7202356B2 (en) * 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
EP3260545B1 (en) 2009-04-20 2020-07-01 Monsanto Technology LLC Multiple virus resistance in plants
CA2714790C (en) 2009-09-17 2018-09-04 Commonwealth Scientific And Industrial Research Organisation Wheat plants with immunity to wsmv
BR112013031723B1 (pt) 2011-06-10 2020-10-13 Temasek Life Sciences Laboratory Limited construção de polinucleotídeo, método para transformação de uma célula fúngica e promotor isolado
WO2014142747A1 (en) 2013-03-14 2014-09-18 Temasek Life Sciences Laboratorey Limited Polynucleotide sequences from rhodosporidium and rhodotor ula and use thereof
MY182911A (en) 2014-09-08 2021-02-05 Temasek Life Sciences Laboratory Ltd Methods for efficient production of polyunsaturated fatty acids (pufa) in rhodosporidium and rhodotorula species

Also Published As

Publication number Publication date
EP3411485A1 (en) 2018-12-12
US20200377898A1 (en) 2020-12-03
WO2017135895A1 (en) 2017-08-10
EP3411485A4 (en) 2019-10-02
CN109072247A (zh) 2018-12-21
EP3411485B1 (en) 2020-12-23
US11254943B2 (en) 2022-02-22
CN109072247B (zh) 2022-06-03
DK3411485T3 (da) 2021-03-29

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]