BR112018009515A2 - sistema e métodos de biocontenção/biocontrole - Google Patents

sistema e métodos de biocontenção/biocontrole

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Publication number
BR112018009515A2
BR112018009515A2 BR112018009515A BR112018009515A BR112018009515A2 BR 112018009515 A2 BR112018009515 A2 BR 112018009515A2 BR 112018009515 A BR112018009515 A BR 112018009515A BR 112018009515 A BR112018009515 A BR 112018009515A BR 112018009515 A2 BR112018009515 A2 BR 112018009515A2
Authority
BR
Brazil
Prior art keywords
coding region
silent mutation
transcription activator
overexpression
biocontainment
Prior art date
Application number
BR112018009515A
Other languages
English (en)
Inventor
Maselko Maciej
Smanski Mike
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of BR112018009515A2 publication Critical patent/BR112018009515A2/pt

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
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    • C12N2510/00—Genetically modified cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

essa divulgação descreve, em um aspecto, uma célula que inclui um sistema de biocontenção. em geral, o sistema de biocontenção inclui uma região codificadora cuja superexpressão diminui o crescimento da célula, uma região reguladora de transcrição que inclui uma mutação silenciosa e está operativamente ligada a montante da região codificadora e um polinucleotideo que codifica um ativador de transcrição programável modificado para se ligar à região reguladora de transcrição na ausência da mutação silenciosa. assim, na ausência da mutação silenciosa, o ativador de transcrição programável induz à superexpressão da região codificadora; na presença da mutação silenciosa, o ativador de transcrição programável não inicia a superexpressão da região codificadora.
BR112018009515A 2015-11-11 2016-11-10 sistema e métodos de biocontenção/biocontrole BR112018009515A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562253954P 2015-11-11 2015-11-11
PCT/US2016/061297 WO2017083501A1 (en) 2015-11-11 2016-11-10 Biocontainment/biocontrol system and methods

Publications (1)

Publication Number Publication Date
BR112018009515A2 true BR112018009515A2 (pt) 2018-11-06

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ID=58695324

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BR112018009515A BR112018009515A2 (pt) 2015-11-11 2016-11-10 sistema e métodos de biocontenção/biocontrole

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US (2) US11718858B2 (pt)
AU (2) AU2016353057C1 (pt)
BR (1) BR112018009515A2 (pt)
WO (1) WO2017083501A1 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11718858B2 (en) 2015-11-11 2023-08-08 Regents Of The University Of Minnesota Biocontainment/biocontrol system and methods
US11834665B2 (en) 2017-05-10 2023-12-05 Regents Of The University Of Minnesota Programmable transcription factors and methods

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7001733B1 (en) * 1998-05-12 2006-02-21 Rigel Pharmaceuticals, Inc. Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement
TW200827442A (en) * 2006-11-03 2008-07-01 Nat Health Research Institutes Method for amplifying a flavivirus cDNA in a prokaryotic cell
ES2741951T3 (es) 2012-12-17 2020-02-12 Harvard College Modificación por ingeniería genética del genoma humano guiada por ARN
GB201223097D0 (en) * 2012-12-20 2013-02-06 Max Planck Gesellschaft Stable transformation of a population and a method of biocontainment using haploinsufficiency and underdominance principles
EP4074330A1 (en) * 2013-09-05 2022-10-19 Massachusetts Institute of Technology Tuning microbial populations with programmable nucleases
WO2015084802A1 (en) 2013-12-02 2015-06-11 Regents Of The University Of Minnesota Rna amplification and oligonucleotide library preparation
US11718858B2 (en) 2015-11-11 2023-08-08 Regents Of The University Of Minnesota Biocontainment/biocontrol system and methods
US11834665B2 (en) 2017-05-10 2023-12-05 Regents Of The University Of Minnesota Programmable transcription factors and methods
WO2020101947A2 (en) 2018-11-05 2020-05-22 Regents Of The University Of Minnesota Systems and breeding methods for pest control
WO2021076342A2 (en) 2019-10-02 2021-04-22 Regents Of The University Of Minnesota Systems and methods for batch cultivation of non-transgenic heterogametes
WO2021087319A1 (en) 2019-10-31 2021-05-06 Regents Of The University Of Minnesota Systems and methods for generating genetic incompatibility

Also Published As

Publication number Publication date
AU2016353057B2 (en) 2020-12-03
AU2016353057C1 (en) 2021-06-17
US20180327762A1 (en) 2018-11-15
US20240093215A1 (en) 2024-03-21
AU2016353057A1 (en) 2018-05-31
US11718858B2 (en) 2023-08-08
AU2021201313A1 (en) 2021-03-18
AU2021201313B2 (en) 2021-08-05
WO2017083501A1 (en) 2017-05-18

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