BR112016004375A2 - acylamino acids production method - Google Patents

acylamino acids production method

Info

Publication number
BR112016004375A2
BR112016004375A2 BR112016004375A BR112016004375A BR112016004375A2 BR 112016004375 A2 BR112016004375 A2 BR 112016004375A2 BR 112016004375 A BR112016004375 A BR 112016004375A BR 112016004375 A BR112016004375 A BR 112016004375A BR 112016004375 A2 BR112016004375 A2 BR 112016004375A2
Authority
BR
Brazil
Prior art keywords
amino acid
acyltransferase
recombinant
production method
acid
Prior art date
Application number
BR112016004375A
Other languages
Portuguese (pt)
Inventor
Wolter Jan
Corthals Jasmin
Grammann Katrin
Reinecke Liv
Olfert Maik
Potgrave Nicole
Schaffer Steffen
Haas Thomas
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Publication of BR112016004375A2 publication Critical patent/BR112016004375A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01065—Bile acid-CoA:amino acid N-acyltransferase (2.3.1.65)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01086—Fatty-acyl-CoA synthase (2.3.1.86)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

resumo da patente de invenção para: "método de produção de acilaminoácidos". a presente invenção refere-se a uma célula que expressa uma aminoácido n-aciltransferase, a qual é de preferência recombinante, e uma acil-coa sintetase, a qual é preferencialmente recombinante, tendo a célula uma capacidade de degradação de ácidos gordos reduzida, a um método de produção de acilaminoácidos que compreende a etapa de pôr em contato um aminoácido e um acil-coa na presença de uma aminoácido n-aciltransferase, a qual é de preferência isolada e/ou recombinante, em que a aminoácido n-aciltransferase é de preferência uma aminoácido n-aciltransferase humana, ou a cultura da célula e uma mistura reacional composta por uma aminoácido n-aciltransferase, a qual é de preferência isolada e/ou recombinante, uma acil-coa sintetase, a qual é de preferência isolada e/ou recombinante, um aminoácido e quer ácido gordo-coa ou um ácido gordo e uma acil-coa sintetase.Patent Summary for: "Acylamino Acid Production Method". The present invention relates to a cell expressing an amino acid n-acyltransferase, which is preferably recombinant, and an acylcoacetetase, which is preferably recombinant, the cell having a reduced fatty acid degradation capacity. an acylamino acid production method comprising the step of contacting an amino acid and an acylco in the presence of an amino acid n-acyltransferase, which is preferably isolated and / or recombinant, wherein the amino acid n-acyltransferase is preferably a human n-acyltransferase amino acid, or cell culture and a reaction mixture composed of an n-acyltransferase amino acid, which is preferably isolated and / or recombinant, an acylcoacetetase, which is preferably isolated and / or recombinant, an amino acid and either fatty acid or a fatty acid and an acylco synthetase.

BR112016004375A 2013-08-27 2014-08-25 acylamino acids production method BR112016004375A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20130181841 EP2843043A1 (en) 2013-08-27 2013-08-27 A method for producing acyl amino acids
EP20140169650 EP2842542A1 (en) 2013-08-27 2014-05-23 A method for producing acyl amino acids
PCT/EP2014/067990 WO2015028423A1 (en) 2013-08-27 2014-08-25 A method for producing acyl amino acids

Publications (1)

Publication Number Publication Date
BR112016004375A2 true BR112016004375A2 (en) 2017-10-17

Family

ID=49029032

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016004375A BR112016004375A2 (en) 2013-08-27 2014-08-25 acylamino acids production method

Country Status (6)

Country Link
US (1) US20160272950A1 (en)
EP (3) EP2843043A1 (en)
JP (1) JP2016528904A (en)
CN (1) CN105813625A (en)
BR (1) BR112016004375A2 (en)
WO (1) WO2015028423A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
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US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
EP2746400A1 (en) 2012-12-21 2014-06-25 Evonik Industries AG Preparation of amines and diamines from a carboxylic acid or dicarboxylic acid or a monoester thereof
EP2759598A1 (en) 2013-01-24 2014-07-30 Evonik Industries AG Process for preparing alpha, omega alkanediols
EP2944697A1 (en) 2014-05-13 2015-11-18 Evonik Degussa GmbH Method of producing nylon
ES2937642T3 (en) * 2014-06-11 2023-03-30 Univ Duke Compositions and methods for rapid and dynamic flow control using synthetic metabolic valves
EP3081639A1 (en) * 2015-04-14 2016-10-19 Evonik Degussa GmbH Acyl amino acid production
EP3091080A1 (en) * 2015-05-05 2016-11-09 Evonik Degussa GmbH Process for producing acyl amino acids employing lipases
US10626424B2 (en) * 2015-07-22 2020-04-21 Dupont Industrial Biosciences Usa, Llc High level production of long-chain dicarboxylic acids with microbes
CN108473967B (en) 2015-12-17 2022-03-29 赢创运营有限公司 Genetically modified acetogenic cells
CN109790106A (en) 2016-07-27 2019-05-21 赢创德固赛有限公司 N- acetylhomoserine
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP6878607B2 (en) 2017-02-21 2021-05-26 デューク ユニバーシティ Compositions and Methods for Robust Dynamic Metabolic Control
EP3585798A4 (en) 2017-02-27 2020-10-14 Duke University In vivo protein n-acylation
JP7306379B2 (en) 2018-04-13 2023-07-11 味の素株式会社 Method for producing N-acyl-amino group-containing compound
US11203744B2 (en) 2018-06-21 2021-12-21 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control
CN114761554A (en) 2019-10-10 2022-07-15 味之素株式会社 Modified enzyme having N-acylation activity
KR102207867B1 (en) * 2020-01-21 2021-01-26 씨제이제일제당 주식회사 Method for producing L-amino acids using microorganisms containing NADP dependent glyceraldehyde-3-phosphate dehydrogenase

Family Cites Families (12)

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JPS5141602B2 (en) 1973-12-12 1976-11-11
GB8918709D0 (en) * 1989-08-16 1989-09-27 Unilever Plc Cosmetic composition
DE69225631T2 (en) * 1991-02-19 1998-12-17 Nat Food Research Inst Ministr Acylamino acid compounds and process for their preparation
US5734070A (en) 1994-02-17 1998-03-31 Degussa Aktiengesellschaft Hardening of unsaturated fats, fatty acids or fatty acid esters
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
RU2212447C2 (en) 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Strain escherichia coli as producer of amino acid (variants) and method for preparing amino acid (variants)
WO2008131002A2 (en) * 2007-04-16 2008-10-30 Modular Genetics, Inc. Generation of acyl amino acids
BRPI1009980A2 (en) * 2009-05-01 2015-08-25 Univ California Fatty acid ester products from biomass polymers
EP2415451A1 (en) * 2010-08-05 2012-02-08 KPSS-Kao Professional Salon Services GmbH Cleansing composition
EP3222713A1 (en) * 2010-08-24 2017-09-27 North-West University Recombinant therapeutic glycine n-acyltransferase
WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2970928A4 (en) * 2013-03-15 2017-02-15 Modular Genetics, Inc. Generation of acyl amino acids

Also Published As

Publication number Publication date
US20160272950A1 (en) 2016-09-22
EP2842542A1 (en) 2015-03-04
EP3038595B1 (en) 2019-08-14
CN105813625A (en) 2016-07-27
JP2016528904A (en) 2016-09-23
EP2843043A1 (en) 2015-03-04
EP3038595A1 (en) 2016-07-06
WO2015028423A1 (en) 2015-03-05

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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B350 Update of information on the portal [chapter 15.35 patent gazette]