BR112015032121A2 - BETA-PRIME SUBUNIT (B' SUBUNIT) MUTANT OF RNA POLYMERASE, MICROORGANISM OF THE GENUS CORYNEBACTERIUM AND METHOD FOR PRODUCING L-LYSINE - Google Patents

BETA-PRIME SUBUNIT (B' SUBUNIT) MUTANT OF RNA POLYMERASE, MICROORGANISM OF THE GENUS CORYNEBACTERIUM AND METHOD FOR PRODUCING L-LYSINE

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Publication number
BR112015032121A2
BR112015032121A2 BR112015032121A BR112015032121A BR112015032121A2 BR 112015032121 A2 BR112015032121 A2 BR 112015032121A2 BR 112015032121 A BR112015032121 A BR 112015032121A BR 112015032121 A BR112015032121 A BR 112015032121A BR 112015032121 A2 BR112015032121 A2 BR 112015032121A2
Authority
BR
Brazil
Prior art keywords
subunit
microorganism
lysine
mutant
producing
Prior art date
Application number
BR112015032121A
Other languages
Portuguese (pt)
Other versions
BR112015032121B1 (en
Inventor
Hee Park Sang
Ok Moon Jun
Ho Lee Kwang
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of BR112015032121A2 publication Critical patent/BR112015032121A2/en
Publication of BR112015032121B1 publication Critical patent/BR112015032121B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Abstract

MUTANTE DE SUBUNIDADE BETA-PRIME (SUBUNIDADE B') DE RNA POLIMERASE, POLINUCLEOTÍDEO, VETOR, MICRORGANISMO DO GÊNERO CORYNEBACTERIUM E MÉTODO PARA PRODUZIR L-LISINA. Trata-se de um mutante de subunidade beta-prime de RNA polimerase, um microrganismo do gênero Corynebacterium que inclui um polinucleotídeo que codifica o mesmo, e um método para produzir L-lisina cultivando-se o mesmo.BETA-PRIME SUBUNIT (B' SUBUNIT) MUTANT OF RNA POLYMERASE, POLYNUCLEOTIDE, VECTOR, MICROORGANISM OF THE GENUS CORYNEBACTERIUM AND METHOD FOR PRODUCING L-LYSINE. This is an RNA polymerase beta-prime subunit mutant, a microorganism of the genus Corynebacterium that includes a polynucleotide encoding the same, and a method for producing L-lysine by culturing the same.

BR112015032121-6A 2013-06-25 2014-06-24 RNA POLYMERASE BETA-PRIME SUBUNIT (?) SUBUNIT MUTANT, MICROORGANISM OF THE GENUS CORYNEBACTERIUM AND METHOD FOR PRODUCING L-LYSINE BR112015032121B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020130073309A KR101594156B1 (en) 2013-06-25 2013-06-25 A microorganism having enhanced L-lysine productivity and a method of producing L-lysine using the same
KR10-2013-0073309 2013-06-25
PCT/KR2014/005576 WO2014208981A1 (en) 2013-06-25 2014-06-24 Microorganism with enhanced l-lysine productivity and method for producing l-lysine by using same

Publications (2)

Publication Number Publication Date
BR112015032121A2 true BR112015032121A2 (en) 2017-08-29
BR112015032121B1 BR112015032121B1 (en) 2022-12-27

Family

ID=52142246

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015032121-6A BR112015032121B1 (en) 2013-06-25 2014-06-24 RNA POLYMERASE BETA-PRIME SUBUNIT (?) SUBUNIT MUTANT, MICROORGANISM OF THE GENUS CORYNEBACTERIUM AND METHOD FOR PRODUCING L-LYSINE

Country Status (10)

Country Link
US (1) US9758771B2 (en)
EP (1) EP3015546B1 (en)
JP (1) JP6195668B2 (en)
KR (1) KR101594156B1 (en)
CN (2) CN105658793A (en)
BR (1) BR112015032121B1 (en)
ES (1) ES2802949T3 (en)
HU (1) HUE050834T2 (en)
PL (1) PL3015546T3 (en)
WO (1) WO2014208981A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101793328B1 (en) * 2015-07-03 2017-11-03 씨제이제일제당 (주) A microorganism having enhanced L-lysine productivity and a method of producing L-lysine using the same
US11306319B2 (en) 2015-10-30 2022-04-19 Danisco Us Inc. Enhanced protein expression and methods thereof
KR102624718B1 (en) 2018-08-07 2024-01-12 씨제이제일제당 주식회사 Nucleic acid molecule containing a variant RPOC coding sequence
AU2020414201B2 (en) * 2019-12-23 2023-10-05 Cj Cheiljedang Corporation Microorganism for producing L-amino acid having increased cytochrome C activity, and L-amino acid production method using same
KR102389327B1 (en) * 2020-07-01 2022-04-20 씨제이제일제당 (주) Microorganism having inhanced activity of 3-methyl-2-oxobutanoate hydroxymethyltransferase and uses thereof
CN112608911B (en) * 2020-12-18 2022-07-05 湖州中科工业生物技术中心 RNA polymerase mutant and application thereof
KR102254209B1 (en) * 2021-01-15 2021-05-18 씨제이제일제당 (주) Novel DNA polymerase Ⅲ subunits gamma and tau variant and a method for producing L-lysine using the same
KR102314882B1 (en) * 2021-01-29 2021-10-19 씨제이제일제당 (주) Novel membrane protein TerC variant and a method for producing L-lysine using the same
KR102344057B1 (en) * 2021-01-29 2021-12-27 씨제이제일제당 (주) Novel protein variant and a method for producing L-lysine using the same
KR102314885B1 (en) * 2021-04-12 2021-10-18 씨제이제일제당 (주) Novel protein variant and a method for producing L-lysine using the same
KR102303747B1 (en) * 2021-04-12 2021-09-16 씨제이제일제당 (주) Novel major facilitator superfamily permease variant and a method for producing L-lysine using the same

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
ATE383419T1 (en) * 2001-12-29 2008-01-15 Novozymes As MUTANE EUBACTERIAL RNA POLYMERASE FOR INCREASE HETEROLOGUE GENE EXPRESSION
AU2007295159B2 (en) 2006-09-15 2012-09-13 Cj Cheiljedang Corporation A Corynebacteria having enhanced L-lysine productivity and a method of producing L-lysine using the same
JP5048996B2 (en) 2006-11-10 2012-10-17 昭和電工株式会社 Wear-resistant aluminum alloy material excellent in workability and method for producing the same
KR100838038B1 (en) * 2006-12-29 2008-06-12 씨제이제일제당 (주) - - a microorganism of corynebacterium genus having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR20070057093A (en) 2007-01-16 2007-06-04 씨제이 주식회사 A microorganism of corynebacterium genus having enhanced l-lysine productivity resistant to kanamycin and a method of producing l-lysine using the same
KR101012590B1 (en) * 2008-06-25 2011-02-07 씨제이제일제당 (주) A corynebacterium having enhanced L-lysine productivity and a method of producing L-lysine using the corynebacterium
BR112012011990A2 (en) 2009-11-18 2015-09-29 Myriant Corp non-naturally occurring microorganism and method for producing succinic acid

Also Published As

Publication number Publication date
WO2014208981A1 (en) 2014-12-31
US20160230151A1 (en) 2016-08-11
CN110423734A (en) 2019-11-08
US9758771B2 (en) 2017-09-12
JP6195668B2 (en) 2017-09-13
ES2802949T3 (en) 2021-01-22
CN110423734B (en) 2023-10-24
EP3015546B1 (en) 2020-05-27
PL3015546T3 (en) 2020-11-16
HUE050834T2 (en) 2021-01-28
BR112015032121B1 (en) 2022-12-27
KR20150000773A (en) 2015-01-05
EP3015546A1 (en) 2016-05-04
EP3015546A4 (en) 2017-01-04
KR101594156B1 (en) 2016-02-15
CN105658793A (en) 2016-06-08
JP2016523543A (en) 2016-08-12

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 24/06/2014, OBSERVADAS AS CONDICOES LEGAIS