BR112015025264A2 - métodos e aparelhos para sintetizar ácidos nucleicos - Google Patents
métodos e aparelhos para sintetizar ácidos nucleicosInfo
- Publication number
- BR112015025264A2 BR112015025264A2 BR112015025264A BR112015025264A BR112015025264A2 BR 112015025264 A2 BR112015025264 A2 BR 112015025264A2 BR 112015025264 A BR112015025264 A BR 112015025264A BR 112015025264 A BR112015025264 A BR 112015025264A BR 112015025264 A2 BR112015025264 A2 BR 112015025264A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- nucleic acids
- synthesizing nucleic
- synthesizing
- acids
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07019—Polynucleotide adenylyltransferase (2.7.7.19)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361807327P | 2013-04-02 | 2013-04-02 | |
| US201361891162P | 2013-10-15 | 2013-10-15 | |
| US14/056,687 US8808989B1 (en) | 2013-04-02 | 2013-10-17 | Methods and apparatus for synthesizing nucleic acids |
| PCT/US2014/033811 WO2014165864A2 (en) | 2013-04-02 | 2014-04-11 | Methods and apparatus for synthesizing nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112015025264A2 true BR112015025264A2 (pt) | 2017-07-18 |
Family
ID=51301627
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015025264A BR112015025264A2 (pt) | 2013-04-02 | 2014-04-11 | métodos e aparelhos para sintetizar ácidos nucleicos |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US8808989B1 (pt) |
| EP (3) | EP2796552B1 (pt) |
| AU (1) | AU2014247928B2 (pt) |
| BR (1) | BR112015025264A2 (pt) |
| CA (1) | CA2908690C (pt) |
| DK (1) | DK2796552T3 (pt) |
| WO (2) | WO2014165864A2 (pt) |
Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11331643B2 (en) | 2013-04-02 | 2022-05-17 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US9279149B2 (en) * | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US8808989B1 (en) * | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US10683536B2 (en) | 2013-04-02 | 2020-06-16 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US9771613B2 (en) | 2013-04-02 | 2017-09-26 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acid |
| US11384377B2 (en) | 2013-04-02 | 2022-07-12 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| CA2916660C (en) | 2013-06-25 | 2022-05-17 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| WO2016028803A2 (en) * | 2014-08-18 | 2016-02-25 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| US11390858B2 (en) | 2014-10-20 | 2022-07-19 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| US10774316B2 (en) | 2014-10-20 | 2020-09-15 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| US10059929B2 (en) | 2014-10-20 | 2018-08-28 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| WO2016094512A1 (en) | 2014-12-09 | 2016-06-16 | yLIFE TECHNOLOGIES CORPORATION | High efficiency, small volume nucleic acid synthesis |
| GB201502152D0 (en) | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| CN107889503A (zh) * | 2015-04-30 | 2018-04-06 | 库瑞瓦格股份公司 | 固定化的聚(n)聚合酶 |
| WO2016182984A1 (en) * | 2015-05-08 | 2016-11-17 | Centrillion Technology Holdings Corporation | Disulfide-linked reversible terminators |
| US20180291413A1 (en) | 2015-10-06 | 2018-10-11 | Thermo Fisher Scientific Geneart Gmbh | Devices and methods for producing nucleic acids and proteins |
| US10982264B2 (en) | 2016-04-22 | 2021-04-20 | Omniome, Inc. | Nucleic acid sequencing method and system employing enhanced detection of nucleotide-specific ternary complex formation |
| EP3449012B1 (en) | 2016-04-29 | 2020-12-23 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
| CA3029011A1 (en) * | 2016-06-22 | 2017-12-28 | The Charles Stark Draper Laboratory, Inc. | Composition for use in molecular chain synthesis |
| WO2018034780A1 (en) | 2016-08-15 | 2018-02-22 | Omniome, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
| WO2018035134A1 (en) | 2016-08-15 | 2018-02-22 | Omniome, Inc. | Method and system for sequencing nucleic acids |
| WO2018125759A1 (en) | 2016-12-30 | 2018-07-05 | Omniome, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
| WO2018132389A1 (en) | 2017-01-10 | 2018-07-19 | Omniome, Inc. | Polymerases engineered to reduce nucleotide-independent dna binding |
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| AU2017394645B2 (en) | 2017-01-20 | 2020-01-23 | Pacific Biosciences Of California, Inc. | Genotyping by polymerase binding |
| CN110249059B (zh) | 2017-01-20 | 2021-04-13 | 欧姆尼欧美公司 | 核酸的等位基因特异性捕获 |
| EP3571319A1 (en) | 2017-01-20 | 2019-11-27 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
| US11492666B2 (en) | 2017-02-15 | 2022-11-08 | Pacific Biosciences Of California, Inc. | Distinguishing sequences by detecting polymerase dissociation |
| WO2018152323A1 (en) * | 2017-02-17 | 2018-08-23 | Camena Bioscience Limited | Compositions and methods for template-free enzymatic nucleic acid synthesis |
| US20180274001A1 (en) * | 2017-03-21 | 2018-09-27 | Molecular Assemblies, Inc. | Nucleic acid synthesis using dna polymerase theta |
| US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
| US9951385B1 (en) | 2017-04-25 | 2018-04-24 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
| CN110997907A (zh) | 2017-05-26 | 2020-04-10 | 核酸有限公司 | 末端转移酶在核酸合成中的用途 |
| US11174512B2 (en) | 2017-05-31 | 2021-11-16 | Molecular Assemblies, Inc. | Homopolymer encoded nucleic acid memory |
| US11612873B2 (en) * | 2017-05-31 | 2023-03-28 | Molecular Assemblies, Inc. | Homopolymer encoded nucleic acid memory |
| JP2020523997A (ja) | 2017-06-19 | 2020-08-13 | マサチューセッツ インスティテュート オブ テクノロジー | マイクロ流体デバイスを使用した、スケーラブルな並列化された酵素的バイオポリマー合成および改変のための自動化された方法 |
| WO2019030149A1 (en) | 2017-08-07 | 2019-02-14 | Dna Script | VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF |
| GB201714827D0 (en) | 2017-09-14 | 2017-11-01 | Nuclera Nucleics Ltd | Novel use |
| GB201721307D0 (en) | 2017-12-19 | 2018-01-31 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| US10435676B2 (en) | 2018-01-08 | 2019-10-08 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| TW201940695A (zh) * | 2018-01-12 | 2019-10-16 | 英商卡美納生物科學公司 | 用於無模板幾何型酶催化性核酸合成之組成物和方法 |
| KR20200139671A (ko) | 2018-02-06 | 2020-12-14 | 옴니옴 인코포레이티드 | 핵산 프라이머 연장을 위한 조성물 및 기술 |
| EP4234718A3 (en) | 2018-04-26 | 2023-11-29 | Pacific Biosciences Of California, Inc. | Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes |
| GB2574197B (en) * | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| US11339428B2 (en) | 2018-05-31 | 2022-05-24 | Pacific Biosciences Of California, Inc. | Increased signal to noise in nucleic acid sequencing |
| US20210147830A1 (en) | 2018-06-29 | 2021-05-20 | Thermo Fisher Scientific Geneart Gmbh | High throughput assembly of nucleic acid molecules |
| WO2020023362A1 (en) | 2018-07-24 | 2020-01-30 | Omniome, Inc. | Serial formation of ternary complex species |
| GB2574746B (en) | 2018-09-17 | 2020-06-03 | Omniome Inc | Engineered polymerases for improved sequencing |
| WO2020101795A1 (en) | 2018-11-15 | 2020-05-22 | Omniome, Inc. | Electronic detection of nucleic acid structure |
| CN109402202A (zh) * | 2018-11-30 | 2019-03-01 | 深圳华大生命科学研究院 | 核酸合成方法 |
| GB2585608B (en) | 2018-12-04 | 2021-11-03 | Omniome Inc | Mixed-phase fluids for nucleic acid sequencing and other analytical assays |
| AU2019398307A1 (en) | 2018-12-13 | 2021-06-10 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| US11041199B2 (en) | 2018-12-20 | 2021-06-22 | Omniome, Inc. | Temperature control for analysis of nucleic acids and other analytes |
| EP3906317A1 (en) * | 2019-01-03 | 2021-11-10 | DNA Script | One pot synthesis of sets of oligonucleotides |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3924500A1 (en) | 2019-02-12 | 2021-12-22 | DNA Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides |
| EP3924513B1 (en) | 2019-02-14 | 2023-04-12 | Pacific Biosciences of California, Inc. | Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| EP4045683A1 (en) | 2019-10-18 | 2022-08-24 | Omniome, Inc. | Methods and compositions for capping nucleic acids |
| US20210139867A1 (en) | 2019-11-08 | 2021-05-13 | Omniome, Inc. | Engineered polymerases for improved sequencing by binding |
| AU2020384271A1 (en) * | 2019-11-13 | 2022-06-23 | Dna Script | High efficiency template-free enzymatic synthesis of polynucleotides |
| DE202019106695U1 (de) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System zur Sequenzierung von Nukleinsäuren in Fluidschaum |
| DE202019106694U1 (de) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System zur Sequenzierung von Nukleinsäuren in Fluidschaum |
| CN115243787A (zh) * | 2019-12-30 | 2022-10-25 | 源点生物科技股份有限公司 | 使用酶制备核酸序列的装置及方法 |
| TWI732405B (zh) * | 2019-12-30 | 2021-07-01 | 源點生物科技股份有限公司 | 使用酶製備核酸序列的方法 |
| CA3173798A1 (en) | 2020-03-03 | 2021-09-10 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing double stranded nucleic acids |
| EP4133070A1 (en) * | 2020-04-06 | 2023-02-15 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| WO2021222512A1 (en) | 2020-04-30 | 2021-11-04 | Dimensiongen | Devices and methods for macromolecular manipulation |
| US20230183798A1 (en) | 2020-05-05 | 2023-06-15 | Pacific Biosciences Of California, Inc. | Compositions and methods for modifying polymerase-nucleic acid complexes |
| US11993801B2 (en) | 2020-07-21 | 2024-05-28 | Illumina Singapore Pte. Ltd. | Base-modified nucleotides as substrates for TdT-based enzymatic nucleic acid synthesis |
| WO2022268932A1 (en) | 2021-06-24 | 2022-12-29 | Thermo Fisher Scientific Geneart Gmbh | Systems and methods for single cell analysis |
| CN114410602B (zh) * | 2022-01-28 | 2024-01-19 | 赛纳生物科技(北京)有限公司 | 一种末端脱氧核苷酸转移酶的突变体及其应用 |
| WO2023187214A1 (en) | 2022-03-31 | 2023-10-05 | Ribbon Biolabs Gmbh | Biopolymer synthesis |
| TW202405168A (zh) | 2022-04-08 | 2024-02-01 | 英商葛蘭素史密斯克藍智慧財產發展有限公司 | 製造含有寡核苷酸之聚核苷酸的新穎方法 |
| WO2023220300A1 (en) | 2022-05-11 | 2023-11-16 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| US5516664A (en) * | 1992-12-23 | 1996-05-14 | Hyman; Edward D. | Enzymatic synthesis of repeat regions of oligonucleotides |
| US5472672A (en) * | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
| US5872244A (en) | 1994-09-02 | 1999-02-16 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US6214987B1 (en) | 1994-09-02 | 2001-04-10 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides |
| US5990300A (en) | 1994-09-02 | 1999-11-23 | Andrew C. Hiatt | Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US5808045A (en) | 1994-09-02 | 1998-09-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US6232465B1 (en) | 1994-09-02 | 2001-05-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US5763594A (en) | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US6309836B1 (en) | 1999-10-05 | 2001-10-30 | Marek Kwiatkowski | Compounds for protecting hydroxyls and methods for their use |
| US6458127B1 (en) | 1999-11-22 | 2002-10-01 | Csaba Truckai | Polymer embolic elements with metallic coatings for occlusion of vascular malformations |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| US7201940B1 (en) | 2001-06-12 | 2007-04-10 | Advanced Cardiovascular Systems, Inc. | Method and apparatus for thermal spray processing of medical devices |
| GB0129012D0 (en) | 2001-12-04 | 2002-01-23 | Solexa Ltd | Labelled nucleotides |
| AUPR975201A0 (en) | 2001-12-24 | 2002-01-24 | Unisearch Limited | Enzymatic redox labelling of nucleic acids |
| DE10215035A1 (de) | 2002-04-05 | 2003-10-23 | Roche Diagnostics Gmbh | Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität |
| US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
| US7947817B2 (en) * | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) * | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7208172B2 (en) | 2003-11-03 | 2007-04-24 | Medlogics Device Corporation | Metallic composite coating for delivery of therapeutic agents from the surface of implantable devices |
| ATE463584T1 (de) * | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
| CA2566806A1 (en) * | 2004-05-25 | 2006-01-19 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US8071755B2 (en) * | 2004-05-25 | 2011-12-06 | Helicos Biosciences Corporation | Nucleotide analogs |
| US20080103053A1 (en) * | 2005-11-22 | 2008-05-01 | Helicos Biosciences Corporation | Methods and compositions for sequencing a nucleic acid |
| US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20080026379A1 (en) | 2006-07-31 | 2008-01-31 | Siddiqi Suhaib M | Nucleotide analogs |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US7928207B2 (en) * | 2004-06-28 | 2011-04-19 | Roche Molecular Systems, Inc | Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides |
| US20060127443A1 (en) | 2004-12-09 | 2006-06-15 | Helmus Michael N | Medical devices having vapor deposited nanoporous coatings for controlled therapeutic agent delivery |
| JP4990886B2 (ja) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
| US20080280937A1 (en) * | 2005-08-19 | 2008-11-13 | Christopher Paul Leamon | Ligand Conjugates of Vinca Alkaloids, Analogs, and Derivatives |
| US8152839B2 (en) | 2005-12-19 | 2012-04-10 | Boston Scientific Scimed, Inc. | Embolic coils |
| AU2007260707A1 (en) * | 2006-06-16 | 2007-12-21 | Pacific Biosciences Of California, Inc. | Controlled initiation of primer extension |
| US20100304368A1 (en) * | 2006-09-20 | 2010-12-02 | Dmitry Cherkasov | Components and method for enzymatic synthesis of nucleic acids |
| US7678894B2 (en) * | 2007-05-18 | 2010-03-16 | Helicos Biosciences Corporation | Nucleotide analogs |
| US9163053B2 (en) | 2007-05-18 | 2015-10-20 | Fluidigm Corporation | Nucleotide analogs |
| WO2009124254A1 (en) * | 2008-04-04 | 2009-10-08 | Helicos Biosciences Corporation | Nucleotide analogs |
| CA2725239C (en) | 2008-05-27 | 2019-02-26 | Trilink Biotechnologies | Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid |
| EP2551354A1 (en) | 2011-07-25 | 2013-01-30 | Universität Heidelberg | Functionalization of RNA oligonucleotides |
| US8808989B1 (en) * | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
-
2013
- 2013-10-17 US US14/056,687 patent/US8808989B1/en active Active
-
2014
- 2014-04-11 AU AU2014247928A patent/AU2014247928B2/en active Active
- 2014-04-11 CA CA2908690A patent/CA2908690C/en active Active
- 2014-04-11 WO PCT/US2014/033811 patent/WO2014165864A2/en active Application Filing
- 2014-04-11 BR BR112015025264A patent/BR112015025264A2/pt not_active IP Right Cessation
- 2014-05-23 EP EP14169758.1A patent/EP2796552B1/en active Active
- 2014-05-23 EP EP16179096.9A patent/EP3115462B1/en active Active
- 2014-05-23 WO PCT/IB2014/061673 patent/WO2014162307A2/en active Application Filing
- 2014-05-23 EP EP18185073.6A patent/EP3425053B1/en active Active
- 2014-05-23 DK DK14169758.1T patent/DK2796552T3/en active
- 2014-07-28 US US14/444,440 patent/US10041110B2/en active Active
-
2018
- 2018-07-03 US US16/026,662 patent/US20180305746A1/en not_active Abandoned
-
2021
- 2021-07-12 US US17/373,314 patent/US20210340608A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US10041110B2 (en) | 2018-08-07 |
| EP2796552A3 (en) | 2015-03-04 |
| AU2014247928B2 (en) | 2017-08-24 |
| EP3115462B1 (en) | 2018-07-25 |
| EP2796552A2 (en) | 2014-10-29 |
| CA2908690A1 (en) | 2014-10-09 |
| WO2014162307A8 (en) | 2014-12-11 |
| EP3425053A1 (en) | 2019-01-09 |
| CA2908690C (en) | 2022-09-27 |
| WO2014162307A3 (en) | 2015-04-23 |
| WO2014162307A2 (en) | 2014-10-09 |
| WO2014165864A3 (en) | 2014-11-20 |
| US20210340608A1 (en) | 2021-11-04 |
| WO2014165864A2 (en) | 2014-10-09 |
| AU2014247928A1 (en) | 2015-10-29 |
| US8808989B1 (en) | 2014-08-19 |
| EP2796552B1 (en) | 2016-07-13 |
| EP3425053B1 (en) | 2020-08-05 |
| DK2796552T3 (en) | 2016-09-05 |
| US20180305746A1 (en) | 2018-10-25 |
| EP3115462A1 (en) | 2017-01-11 |
| US20140363851A1 (en) | 2014-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112015025264A2 (pt) | métodos e aparelhos para sintetizar ácidos nucleicos | |
| BR112015023398A2 (pt) | métodos para amplificar ácidos nucléicos | |
| IL245909B (en) | Libraries of nucleic acids and methods for their preparation | |
| DK3234134T3 (da) | Målrettet rna-redigering | |
| BR112016014165A2 (pt) | aparelho de produção aditiva e métodos para operação do mesmo | |
| BR112014024897A2 (pt) | Método para amplificar ácidos nucleicos | |
| GB2537077B (en) | Methods for sequencing nucleic acids | |
| BR112015007125A2 (pt) | aparelho e método | |
| BR112015022857A2 (pt) | sal de omecamtiv mecarbil e processo para preparar sal | |
| BR112016027102A2 (pt) | método e aparelho | |
| DK3094731T3 (da) | Saccharidmodificerede nukleinsyremolekyler | |
| BR112015030034A2 (pt) | aparelho automatizado para transplante capilar e método para transplante de cabelo | |
| BR112015019224A2 (pt) | aparelho, e método | |
| BR112015021083A2 (pt) | método | |
| CL2015001671A1 (es) | Método y dispositivo para producir cobre en bruto | |
| BR112016009916A2 (pt) | Aparelho de injetor e método | |
| BR112015030157A2 (pt) | método e aparelho para pintura eletrostática | |
| BR112016005592A2 (pt) | método para produzir ácidos poliaspárticos | |
| BR112015029076A2 (pt) | método | |
| FI20145654A (fi) | Menetelmä ja laitteisto pitkänomaisten elintarvikkeiden valmistamiseksi | |
| DK2976637T3 (da) | Prøveapparat | |
| DE112014005294A5 (de) | Vorrichtung zur Wärmeerzeugung | |
| DK3047028T3 (da) | Fermenteringsfremgangsmåde | |
| BR112015002671A2 (pt) | método e aparelho | |
| BR112017008394A2 (pt) | aparelho e método para dobrar |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |