BR112015022778B1 - Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja - Google Patents

Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja Download PDF

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Publication number
BR112015022778B1
BR112015022778B1 BR112015022778-3A BR112015022778A BR112015022778B1 BR 112015022778 B1 BR112015022778 B1 BR 112015022778B1 BR 112015022778 A BR112015022778 A BR 112015022778A BR 112015022778 B1 BR112015022778 B1 BR 112015022778B1
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BR
Brazil
Prior art keywords
fad2
soybean
plant
deletion
alleles
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BR112015022778-3A
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English (en)
Portuguese (pt)
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BR112015022778A2 (pt
Inventor
Luc Mathis
Daniel F. Voytas
Feng Zhang
William Haun
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Cellectis
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Publication of BR112015022778A2 publication Critical patent/BR112015022778A2/pt
Publication of BR112015022778B1 publication Critical patent/BR112015022778B1/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112015022778-3A 2013-03-15 2014-03-13 Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja BR112015022778B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790655P 2013-03-15 2013-03-15
US61/790,655 2013-03-15
US201361885213P 2013-10-01 2013-10-01
US61/885,213 2013-10-01
PCT/IB2014/059752 WO2014141147A1 (en) 2013-03-15 2014-03-13 Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes

Publications (2)

Publication Number Publication Date
BR112015022778A2 BR112015022778A2 (pt) 2017-11-07
BR112015022778B1 true BR112015022778B1 (pt) 2023-04-11

Family

ID=50543262

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022778-3A BR112015022778B1 (pt) 2013-03-15 2014-03-13 Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja

Country Status (6)

Country Link
EP (1) EP2966983B1 (https=)
JP (1) JP6591898B2 (https=)
CN (1) CN105246324B (https=)
BR (1) BR112015022778B1 (https=)
CA (1) CA2906406C (https=)
WO (1) WO2014141147A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
WO2017134601A1 (en) * 2016-02-02 2017-08-10 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
EA202091316A1 (ru) * 2016-12-22 2020-08-18 Тулджин Инкорпорейтид Обогащенный олеиновой кислотой растительный организм, имеющий генетически модифицированный fad2, и способ его получения
CN108374007B (zh) * 2018-02-03 2021-09-21 吉林省农业科学院 高油酸转基因大豆事件eb8072外源插入片段侧翼序列及其应用
CN114423281A (zh) * 2019-09-20 2022-04-29 卡里克特公司 基于基因编辑的低植物雌激素大豆
US20230008694A1 (en) * 2019-11-27 2023-01-12 Calyxt, Inc. Tal-effector nucleases for gene editing
CN112680465A (zh) * 2020-12-28 2021-04-20 浙江农林大学 用于改造大豆脂肪酸组分的基因突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6414223B1 (en) * 1998-08-03 2002-07-02 Cargill, Incorporated Plants, seeds and oils having an elevated total monounsaturated fatty acid content
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
EP3202899B1 (en) 2003-01-28 2020-10-21 Cellectis Custom-made meganuclease and use thereof
CN100339481C (zh) * 2005-03-17 2007-09-26 东北师范大学 应用基因沉默技术提高大豆和花生的种子油酸含量的方法
CN102753684B (zh) * 2009-06-22 2014-07-30 国立大学法人佐贺大学 增加大豆油脂中的油酸含量的突变体及其原因基因
CN103002727B (zh) * 2009-07-08 2016-04-27 密苏里大学管委会 利用传统大豆育种技术培育高油酸大豆的方法
WO2011049627A1 (en) * 2009-10-22 2011-04-28 Dow Agrosciences Llc Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
UY33868A (es) * 2011-01-14 2012-04-30 Univ Missouri Un método para desarrollar soja de alto contenido en ácido oleico mediante técnicas de cultivo de soja convencionales
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив

Also Published As

Publication number Publication date
EP2966983B1 (en) 2021-07-28
WO2014141147A1 (en) 2014-09-18
EP2966983A1 (en) 2016-01-20
JP6591898B2 (ja) 2019-10-16
CN105246324B (zh) 2018-08-28
CA2906406C (en) 2023-03-14
BR112015022778A2 (pt) 2017-11-07
CA2906406A1 (en) 2014-09-18
JP2016509865A (ja) 2016-04-04
CN105246324A (zh) 2016-01-13

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