BR112012028558A2 - método para gerar ácido nucleico de fita dupla com uma sequência de sobreposição de fita simples. - Google Patents

método para gerar ácido nucleico de fita dupla com uma sequência de sobreposição de fita simples.

Info

Publication number
BR112012028558A2
BR112012028558A2 BR112012028558A BR112012028558A BR112012028558A2 BR 112012028558 A2 BR112012028558 A2 BR 112012028558A2 BR 112012028558 A BR112012028558 A BR 112012028558A BR 112012028558 A BR112012028558 A BR 112012028558A BR 112012028558 A2 BR112012028558 A2 BR 112012028558A2
Authority
BR
Brazil
Prior art keywords
stranded
nucleic acid
stranded nucleic
sequence
double stranded
Prior art date
Application number
BR112012028558A
Other languages
English (en)
Inventor
Lisby Gorm
Vest Schneider Uffe
Original Assignee
Quantibact As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantibact As filed Critical Quantibact As
Publication of BR112012028558A2 publication Critical patent/BR112012028558A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

método para gerar ácido nucleico de fita dupla com uma sequência de sobreposição de fita simples - a presente invenção refere-se a um método que combina a construção de produtos de amplificação de alvo de fita com uma ou duas sequências de sobreposições fita simples com a produção aprimorada da de tais produtos de amplificação alvo. a(s) sequências(s) de sobreposição de fita simples pode ser usada para captura pós-amplificação e subsequente detecção/manipulação. a(s) sequência(s) de sopreposição de fita simples possibilita a captura/detecção/manipulação sem interferência a partir da fita complementar no produto de amplificação de alvo de fita dupla.
BR112012028558A 2010-05-07 2011-05-06 método para gerar ácido nucleico de fita dupla com uma sequência de sobreposição de fita simples. BR112012028558A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201000403 2010-05-07
DKPA201000843 2010-09-20
PCT/DK2011/050157 WO2011137911A2 (en) 2010-05-07 2011-05-06 Method for generating a double stranded nucleic acid with a single stranded overhang

Publications (1)

Publication Number Publication Date
BR112012028558A2 true BR112012028558A2 (pt) 2019-09-24

Family

ID=44904162

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012028558A BR112012028558A2 (pt) 2010-05-07 2011-05-06 método para gerar ácido nucleico de fita dupla com uma sequência de sobreposição de fita simples.

Country Status (11)

Country Link
US (1) US8859238B2 (pt)
EP (1) EP2566981A2 (pt)
JP (1) JP2013524841A (pt)
CN (1) CN102933720B (pt)
AU (1) AU2011250350A1 (pt)
BR (1) BR112012028558A2 (pt)
CA (1) CA2796856A1 (pt)
IL (1) IL222916A0 (pt)
MX (1) MX2012012908A (pt)
SG (1) SG184946A1 (pt)
WO (1) WO2011137911A2 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2012070618A1 (ja) 2010-11-24 2014-05-19 株式会社カネカ 増幅核酸検出方法及び検出デバイス
JP6219816B2 (ja) * 2012-04-27 2017-10-25 株式会社カネカ 核酸の増幅方法、および、増幅核酸の検出方法
WO2015076356A1 (ja) 2013-11-22 2015-05-28 株式会社カネカ 短鎖ｒｎａの検出方法
US11976273B2 (en) 2017-02-17 2024-05-07 Brandeis University DNA display of folded RNA libraries enabling RNA-SELEX without reverse transcription
WO2019134835A1 (en) * 2018-01-05 2019-07-11 Quotient Suisse Sa Self-assembling diagnostic array platform
US20210079458A1 (en) * 2019-09-12 2021-03-18 National Guard Health Affairs Amplification method of a single stranded dna

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
WO1994021820A1 (en) 1993-03-17 1994-09-29 City Of Hope Nucleic acid sequences having single stranded ends
GB9902971D0 (en) 1999-02-11 1999-03-31 Zeneca Ltd Assay
WO2000060120A2 (en) * 1999-04-02 2000-10-12 Mosaic Technologies Methods of detecting microorganism using immobilized probes
WO2003052132A2 (en) 2001-12-18 2003-06-26 Human Genetic Signatures Pty Ltd Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides
JP4980219B2 (ja) * 2004-09-10 2012-07-18 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド インターカレート型擬似ヌクレオチド（ｉｐｎ）を含有するインターカレーティング核酸（ｉｎａ）を含む増幅ブロッカー
PL1888748T3 (pl) * 2005-05-25 2014-03-31 Tina Holding Aps Stabilne i selektywne tworzenie tripleksów i dupleksów typu hoogsteen przy zastosowaniu skręconych interkalujących kwasów nukleinowych (tina) i proces wytwarzania tina
US8008019B2 (en) * 2007-11-28 2011-08-30 Luminex Molecular Diagnostics Use of dual-tags for the evaluation of genomic variable repeat regions
PT2265729E (pt) * 2008-03-10 2013-10-10 Quantibact As Amplificação e sequenciação alvo com iniciadores compreendendo unidades de monómeros que formam triplexes

Also Published As

Publication number Publication date
JP2013524841A (ja) 2013-06-20
US20130052652A1 (en) 2013-02-28
SG184946A1 (en) 2012-11-29
CA2796856A1 (en) 2011-11-10
WO2011137911A2 (en) 2011-11-10
CN102933720B (zh) 2015-03-18
EP2566981A2 (en) 2013-03-13
US8859238B2 (en) 2014-10-14
AU2011250350A1 (en) 2012-11-08
WO2011137911A3 (en) 2011-12-22
MX2012012908A (es) 2013-05-01
IL222916A0 (en) 2012-12-31
CN102933720A (zh) 2013-02-13

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2543 DE 01-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.