BR102012032927A2 - PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVES ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN CALLS PROBIIOTIC BIOACTIVE EXTRACTS (EBP) FOR USE IN COSMETIC PRODUCTS, BIOACTIC PRODUCTS, AND ALTERNATIVE PRODUCTS HUMAN AND ANIMAL BODY - Google Patents

Abstract

PROCESS OF PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVES ADDED OF AROMATIC AND ANTIMICROBIAL ESSENTIAL OILS, CALLED PROBIOTIC BIOACTIVE EXTRACTS (EBP) FOR USE IN COSMETIC SUPPLY, BOTTLE AND BODY TREATMENT PRODUCTS OF THE HUMAN AND ANIMAL BODY. The main basis of this patent is the formation of a living consortium between several microorganisms stabilized in order to reach the final application of the product with all the effects of living bioactive substances or useful substances resulting from the fermentation result of the microorganisms with substrates such as Grape, Lemon, Milk, soy and fruit juices. For each substrate used in fermentation as well as microorganism species, several substances useful for human health are generated causing effects on human health and this effect can be added in products such as soaps, hair tonics, natural drinks, dermatological products, creams, emulsions among others. This point is crucial to the potentiation of assets and active substances by the process that involves the dynamization of liquid substances with care of time, temperature, agitation and rest, separation of phases, involving a vital energy process.

Description

“PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED OF AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN, CALLS PROBIIOTIC BIOACTIVE EXTRACTS (EBP), FOR USE IN ADMINISTRATIVE COSMETIC PRODUCTS, BUTTERIAL PRODUCTS IN INTERNAL USE OF THE HUMAN AND ANIMAL BODY ”

APPLICATION FIELD

The present patent relates to a process of

production of glycerinated extracts of probiotic bioactives added with aromatic and antimicrobial essential oils for use in cosmetic input, dermatological treatment products, for external use, as well as food and beverage additive for internal use of the human and animal body, from formation of a live consortium between various microorganisms stabilized to reach the final application of the product with all the effects of living bioactives or useful substances from the result of fermentation of microorganisms with substrates such as grapes, lemon, milk , soy and juice, 20 fruit.

Probiotics are defined as “a culture of living microorganisms, which when ingested in sufficient quantity, cause specific and beneficial effects on the consumer, beyond simple basic nutrition” (World Health Organization, 2001).

25 Several specific health benefits have been

proven as relief from chronic inflammatory bowel disease, prevention and treatment of pathogenic diarrhea, urogenital infections and atopic diseases, and immune system improvement. In short, probiotics: significantly increase the nutritional and therapeutic value of food, as there is an increase in the levels of B vitamins and amino acids. Increased absorption of calcium and iron; strengthen the immune system through increased production of protective cells;

have beneficial functional effect on the body, balancing the intestinal flora, acting on cholesterol control and reducing the risk of cancer; They are of particular importance to individuals with lactose intolerance because of the increase in an enzyme that facilitates lactose digestion. According to recent studies, in addition to these functions 10 probiotics also help strengthen the immune system, helping the body create defenses against unwanted bacteria and microorganisms.

Regarding the application of probiotics to the skin we have the first report of Lb. bulgaricus to treat acne and seborrhea through publication 15 in 1912. However, it was not until 1999 that proper scientific technique was used to evaluate some of the potential specific benefits of the skin. Studies have shown that lactic acid bacteria (Streptococcus thermophilus) found in yogurt can increase ceramide production when applied to the skin for 7 days as a cream. This is important because such compounds exert antimicrobial activities against Propionibacterium acnes direct anti-inflammatory activity. Another clinical study shows the topical application of a probiotic lotion containing Enterococcus faecalis probiotic, which reduced 25 inflammatory lesions by more than 50% vs placebo. Certain substances secreted by bacterial strains, such as antimicrobial peptides, have been shown to inhibit P. acnes growth. They have also been shown to secrete a bacteriocin-type inhibitor, a substance capable of inhibiting P. acnes. In addition to antimicrobial activity, they act as immune modulators. Probiotics can provide a protective barrier to the skin, as a physical barrier, through competitive inhibition of binding sites, preventing skin colonization by other pathogens.

The skin is the largest of all organ systems that

They make up our immune system, which serves as a mechanical barrier to infectious problems. The gastrointestinal tract is another important defense system that aims to eliminate the origin problems of food and drink. The lungs are important in their ability to fight the harmful effects of air pollutants and bacteria and viruses. When kept healthy, this network of skin, membranes, cells, enzymes and hormones sends the proper signals and messages to protect the body from invaders, disease and infections.

is Healthy functioning of the immune system is a

integral component of the whole. There is growing evidence that the internal microbiome and the immune system are extremely linked and work together to maintain overall health. Much of our overall health begins in the gut, which is our first line of defense. Probiotics are also responsible for producing B-complex vitamins so that the body, absorbing, produces enzymes that help break down the molecules (digestive system).

In order for probiotics to bring the desired benefits of application both externally (skin) and internally, there is a need for these microorganisms to come alive by the time of application to the human body, and for this a process of development is needed. stabilization thereof. Also the fermentation or bioassay process must be done on special substrates in such a way as to generate in this process specific substances useful for tissue recomposition.

Thus the two key points of this patent lie in the process of stabilization in the liquid phase for microorganisms to come alive, along with bioactive substances until their application, this applied to foods and cosmetics called Preservative Free Preservative Free.

TECHNICAL KNOWLEDGE

Some microorganism-related patents disclose some probiotic stabilization processes, but have noticeable differences from the process disclosed in this patent, as follows:

Patent Application of July 27, 2009, PT1119345 (E) "Encapsulation of Sensitive Liquid Components into a Matrix to Obtain Discrete Shelf-Stable Particles", discloses a process for encapsulating dissolved or dispersed microorganisms or enzymes which allows release of controlled active compounds as needed, however it requires the use of low temperatures for encapsulation, thus limiting the use of technology to 20 chemically and cost-effectively compatible low-temperature processes.

Patent Application KR100878339 (B1), "Methods Of Double Microencapsulation Of Lactobacillus Using Prebiotic Substrates" of 01/2009, deals with a double encapsulation process utilizing probiotic lyophilization and conservation with enteric material. This process, however, requires the use of ultracentrifugation (18000 rpm), which is a limit of this type of technology.

The MX2006PA06735 patent “Continuous MultiMicroencapsulation Process For Improving The Stability And Storage Life Of Biologically Active Ingredients” of 2006, although it can handle different forms of technological use such as powder, liquid, cream or gel, persists in the use of spray drying that can cause variation in the quantity and quality of encapsulated microorganisms, which is clearly a technology limitation.

PL355620 (A1) of May 4, 2004, "Method for Drying Bacteria", presents a process with great operational possibility, being able to deal with a wide variety of microorganisms for different purposes, however the percentage of living organisms is reduced. which causes a limitation in the use of the process.

US2003109025 (A1) of June 12, 2003, entitled "Particles Containing Coated Living Microorganisms and Method for Producing Same", describes a drying method that maintains the viability of microorganisms by using hydrophobic substances, however. uses equipment with hot air flow whose temperature can make microorganisms unfeasible. The use of rotation system is another point that can make the process unfeasible from the point of view of costs for its industrialization.

US Patent 6447823 (B1) of September 10, 2002,

“Liquid Yogurt with Encapsulated Lactic Acid Bacteria and Method for Producing the Same” describes a process that generates stomach acid-resistant capsules soluble in the intestinal solution, the capsules are also evenly distributed in liquid yogurt, 25 however the process described is only for lactic acid bacteria which is a limitation of technology.

The limitations of the previous processes mentioned above generate the need for the development of new processes that control the problems arising from these limitations. Overcoming the limits of the technique disclosed by the present invention are formulations that allow more than 4 microorganisms to be placed in a consortium-stabilized form on different substrates, whether food, cosmetic or otherwise, and to concentrate them in 5 matrices. formed by polysaccharides, through encapsulation, making use of polysaccharides that are products of the consortium's own metabolism, as well as others added to the process. SUMMARY FOR INVENTION

The process of producing probiotic glycerinated bioactive extracts added with aromatic and antimicrobial essential oils, called Probiotic Bioactive Extracts (EBP), described in this patent is suitable for use in cosmetic, dermatological and food products, whose microorganisms are presented. live and contain bioactive substances that exerts an immunomodulatory action, forming an effective biological barrier for the recomposition of tissues of the human body and generating moisturizing effect as well as assisting in the protection of the skin microbiota, leaving the skin smooth. Bioactive substances are made up of organic acids, vitamins, amino acids and carbohydrates, free of unnatural preservatives and have essential oils important for the effect of product penetration.

The main basis of this patent is the formation of a live consortium between various microorganisms stabilized to reach the final application of the product with all the effects of living bioactive or useful substances from the result of fermentation of microorganisms with substrates such as grape, lemon, milk, soy and fruit juices.

For each substrate used in fermentation as well as microorganism species, various substances useful to human health are generated causing effects on human health and this effect can be added in products such as soaps, hair tonics, natural drinks, dermatological products, creams, emulsions. among others.

This point is crucial to the enhancement of assets and

active substances by the process involving the dynamization of liquid substances with care of time, temperature, agitation and rest, phase separation, involving a vital energy process.

DESCRIPTION OF THE INVENTION

The present invention relates to a manufacturing process of extract based on probiotic microorganisms comprising at least one bacterium and / or yeast from the group of bacteria Yeast formed by Acetobacter aceti, Acetobacter pasteureanus, Enterococcus faecalis, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactobacillus kefirgranum, Lactobacillus paracasei subsp. paracasei, Lactobacillus parakefir, Lactobacillus plantarum, Lactobacillus 20 viridescens, Lactococcus lactis, Streptococcus thermophilus, Lactobacillus diolivorans or Lactobacillus diolivorans-like or Lactobacillus sp. Mesenteroid, Lactococcus lactis subsp. Cremoris, Pediococcus parvulus, Pichia fermentans, Candida 25 delbrueckii, Candida friedricchii, Candida holmii, Candida kefir, Candida krusei, Candida lambica, Candida valida, Cryptococcus humicolus, Debaryomyces hanseni Pichia fermentum Saccharomyces cerevisiae Saccharomyees exigus Saccharomyees fragilis Saccharomyees humalicus Saccharomyees lactis Saccharomyces unisporus Torulaspora delbrueckii use for cosmetic, dermatological, food products, whose microorganisms are alive and contain bioactive substances that exerts an immunomodulatory action, forming an effective biological barrier for recomposition of tissues of the human body and generating moisturizing effect, as well as Helps protect the microbiota of the skin, leaving the skin smooth. Bioactive substances are made up of organic acids, vitamins, amino acids and carbohydrates, free of unnatural preservatives and have essential oils important for the product penetration effect.

The probiotic extract disclosed in this patent should preferably be used as an additive in non-preservative products and petroleum products in the Preservative Free category, ie without the need for preservatives, which have bioactive elements useful for recompose healing and restore the desirable quality of the skin, which is obtained through the following productive steps, illustrated in the flow chart presented in the attached figure, where:

1. Understands the fermentation process by adding the microorganisms and bioassay substrate that

it may be milk, soy, lemon, grape and multiple fruit juices and mixtures thereof;

2. Understands that after fermentation, filtration occurs with porosity ranging from 1 micron to Mech 40; 3. It comprises the extraction of bioactive substances without affecting the living glycerin-based microorganisms, an extraction process that occurs between 10 to 50 days. At this stage essential oils (3A) are added with unwanted microorganism growth controllers and system stabilizers. This point is crucial the potentiation by the addition of extracts of probiotic microorganisms, and essential oils, within a homogenization process that involves the dynamization of liquid substances with care of time, temperature, agitation and rest, phase separation, involving a process of vital energy. The different essential oils to the extract, glycerine and probiotic extracts are placed for heating between 45 ° C and 75 ° C; The biphasic system of joining a time of 10 to 120 minutes is homogenized and given a time for dynamization. The extracts are then added at a temperature of 25 ° C to 65 ° C, plus another dynamization, with a time of 10 to 120 minutes, at which time the oils are absorbed.

4. It comprises the separation of the solid (5) and liquid (6) phases from the centrifugation base (4), where the solid phase (5) is separated, emulsified and a re-added part the liquid phase (6) where the solid phase ( 5) Repositioned ranges from 10 to 50% of the total phases in step 7.

5. The centrifuged liquid phase (6) undergoes a second filtration in step 8, proceeding to the remixing phase (9) and finally to the product packaging (10).

Claims (5)

1. PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED OF AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN, CALLED PROBIOTIC BIOACTIVE EXTRACTS, FOR USE IN ADMINISTRATIVE COSMETIC PRODUCTS IN INTERNAL USE OF THE HUMAN AND ANIMAL BODY, characterized in that it comprises at least one bacterium and / or a yeast from the group of bacteria Yeast formed by Acetobacter aceti, Acetobacter pasteureanus, Enterococcus faecalis, Lactobacillus acidophilus, Lactobacillus delobueckus fermentate, , Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactobacillus kefirgranum, Lactobacillus paracasei subsp. paracasei, Lactobacillus parakefir, Lactobacillus plantarum, Lactobacillus viridescens, Lactococcus lactis, Streptococcus thermophilus, Lactobacillus diolivorans, or Lactobacillus diolivorans-like or Lactobacillus sp. Mesenteroid, Lactococcus lactis subsp. Cremoris, Pediococcus parvulus, Pichia fermentans, Candida delbrueckii, Candida friedricchii, Candida holmii, Candida kefir, Candida krusei, Candida lambica, Candida valida, Cryptococcus humicolus, Debaryomyces hansenii, Kazachstania exigua, Kazachstania unispea Pichia fermentum Saccharomyces cerevisiae Saccharomyces exigus Saccharomyees fragilis Saccharomyees humalicus Saccharomyees lactis Saecharomyces unisporus Torulaspora delbrueckii fermentative process of microorganisms with substrates such as grapes, milk, lemon, soy or fruit juices, produces extract applied in cosmetic and natural foods without preservatives, which has useful bioactive elements to restore healing and restore the desirable quality of the skin. 2. PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIANS, CALLED PROBIOTIC BIOACTIVE EXTRACTS, FOR USE IN ADDITIONAL COSMETIC PRODUCTS, BIOTIC PRODUCTS INTERNAL USE OF THE HUMAN AND ANIMAL BODY according to Claim 1, characterized in that the production process is comprised of the fermentative process steps adding the microorganisms and the bio-augmentation substrate; after fermentation filtration occurs with porosity ranging from 0.1 micron to Mech 40; extraction of bioactive substances without affecting glycerin-based living microorganisms, an extractive process that occurs between 10 and 50 days; whereas at this stage essential oils are added with unwanted microorganism growth controllers and system stabilizers; as well as the separation of solid and liquid phases based on centrifugation, where the solid phase is separated, emulsified and a part re-added to the liquid phase for remixing and packaging of the product. 3. PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIANS, CALLED PROBIOTIC BIOACTIVE EXTRACTS, FOR USE IN ADDITIONAL COSMETIC PRODUCTS, BIOACTIC PRODUCTS INTERNAL USE OF THE HUMAN AND ANIMAL BODY according to Claim 1, characterized in that the active and active substances are stabilized and enhanced by the process involving the dynamization of liquid substances with care of time, temperature, agitation and rest, phase separation, involving a vital energy process of the production process, aiming at stabilization ensuring the arrival to the consumer of the benefits of probiotic and active substances. 4. PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED OF AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN, CALLED PROBIOTIC BIOACTIVE EXTRACTS, FOR USE IN ADDITIONAL COSMETIC PRODUCTS, BIOTIC PRODUCTS INTERNAL USE OF THE HUMAN AND ANIMAL BODY according to Claim 1, characterized in that the specific microorganisms in the fermentation process produce correlation with fermentation substrates such as milk, grape juice, soy, lemon and fruit juices that generate bioactive substances. useful for alleviating chronic inflammatory bowel diseases, prevention and treatment of pathogenic diarrhea, urogenital infections and atopic diseases, as well as improving the immune system; 5. PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVE ADDITIONS ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIANS, CALLED PROBIOTIC BIOACTIVE EXTRACTS, FOR USE IN ADDITIONAL COSMETIC PRODUCTS, BIOTIC PRODUCTS INTERNAL USE OF THE HUMAN AND ANIMAL BODY, according to Claims 1 and 2, characterized in that essential oils and vegetable glycerins are used as stabilizers in addition to the process of productive steps, where the active and active substances are stabilized and enhanced by the process. It involves the dynamization of liquid substances with care of time, temperature, agitation and rest, phase separation, involving a vital energy process.