BR0212145A - Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso - Google Patents

Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso

Info

Publication number
BR0212145A
BR0212145A BR0212145-0A BR0212145A BR0212145A BR 0212145 A BR0212145 A BR 0212145A BR 0212145 A BR0212145 A BR 0212145A BR 0212145 A BR0212145 A BR 0212145A
Authority
BR
Brazil
Prior art keywords
proteins
methods
silencing
plant
proteins involved
Prior art date
Application number
BR0212145-0A
Other languages
English (en)
Inventor
Erik T Mirkov
Ivan L Ingelbrecht
Original Assignee
Texas A & M Univ Sys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A & M Univ Sys filed Critical Texas A & M Univ Sys
Publication of BR0212145A publication Critical patent/BR0212145A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Abstract

"ISOLAMENTO DE PROTEìNAS ENVOLVIDAS NO SILENCIAMENTO GêNICO PóS-TRANSCRICIONAL E MéTODOS DE USO". A presente invenção inclui um método para a detecção e isolamento de proteínas da cana-de-açúcar que interagem com as proteínas HC-Pro e P1 da SRMV e com outras proteìnas envolvidas no silenciamento de genes, particularmente na cana-de-açúcar. O método utiliza um ensaio duplo híbrido como uma HC-Pro, P1, ou outra proteína relacionada ao silenciamento, contendo uma proteína isca e uma proteína presa contendo um polipeptídio codificado por uma molécula de DNA em uma biblioteca CDNA. O método também inclui a identificação de falsos positivos através de ensaios duplo híbrido reversos e utilizando técnicas e in vitro tais como o farwestern blot ou prestes destrutivos nos quais as condições fisiológicas da planta podem ser replicadas. Finalmente, as interações podem ser confirmadas na planta. Algumas novas proteínas utilizadas aqui e descobertas utilizando esses métodos também são identificadas. Também são discutidos métodos para regular o silenciamento em plantas tais como a cana-de-açúcar utilizando proteínas virais e de planta.
BR0212145-0A 2001-08-24 2002-08-26 Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso BR0212145A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31486301P 2001-08-24 2001-08-24
US10/226,715 US7001739B2 (en) 2001-08-24 2002-08-23 Isolation of proteins involved in posttranscriptional gene silencing and methods of use
PCT/US2002/027300 WO2003018809A2 (en) 2001-08-24 2002-08-26 Isolation of proteins involved in posttranscriptional gene silencing and methods of use

Publications (1)

Publication Number Publication Date
BR0212145A true BR0212145A (pt) 2005-08-16

Family

ID=26920809

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0212145-0A BR0212145A (pt) 2001-08-24 2002-08-26 Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso

Country Status (5)

Country Link
US (9) US7001739B2 (pt)
EP (1) EP1419253B1 (pt)
BR (1) BR0212145A (pt)
DE (1) DE60223815D1 (pt)
WO (1) WO2003018809A2 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7001739B2 (en) 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use
FR2884259A1 (fr) * 2005-04-08 2006-10-13 Agronomique Inst Nat Rech Procede de detection d'isolats du virus de la pomme de terre y (pvy) responsables de necroses
DE102006029447A1 (de) * 2006-06-21 2007-12-27 Bayer Schering Pharma Ag Oxo-substituierte Imidazo[1,2b]pyridazine, deren Herstellung und Verwendung als Arzneimittel
AU2008218813B2 (en) 2007-02-20 2014-04-17 Monsanto Technology, Llc Invertebrate microRNAs
KR101347008B1 (ko) 2007-06-27 2014-01-02 에프. 호프만-라 로슈 아게 환자 생리학의 모델링에 기초하여 환자 맞춤형 치료를 개발하는 시스템 및 방법
US8707150B2 (en) * 2008-12-19 2014-04-22 Microsoft Corporation Applying effects to a video in-place in a document
CN103052712B (zh) 2010-05-10 2016-10-12 德克萨斯A&M大学系统 用于在植物中表达基因产物的组合物、生物体、体系和方法
KR101460231B1 (ko) * 2011-08-02 2014-11-10 한국생명공학연구원 식물 이중-하이브리드 시스템을 이용한 단백질 상호작용 검출방법 및 검출키트
CN102363819A (zh) * 2011-11-08 2012-02-29 云南省农业科学院甘蔗研究所 甘蔗条纹花叶病毒检测用引物及其检测方法
AR089830A1 (es) 2012-01-27 2014-09-17 Texas A & M Univ Sys Acido nucleico, vector de expresion, celula bacteriana, celula de planta de citrus y metodo de expresion de un acido nucleico exogeno en la planta de citrus
US11393108B1 (en) 2016-02-26 2022-07-19 Amazon Technologies, Inc. Neighborhood alert mode for triggering multi-device recording, multi-camera locating, and multi-camera event stitching for audio/video recording and communication devices
US10748414B2 (en) 2016-02-26 2020-08-18 A9.Com, Inc. Augmenting and sharing data from audio/video recording and communication devices
US9965934B2 (en) 2016-02-26 2018-05-08 Ring Inc. Sharing video footage from audio/video recording and communication devices for parcel theft deterrence

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040496A (en) * 1995-06-30 2000-03-21 Novartis Finance Corporation Use of translationally altered RNA to confer resistance to maize dwarf mosaic virus and other monocotyledonous plant viruses
US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
US6395962B1 (en) * 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
US7001739B2 (en) 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use

Also Published As

Publication number Publication date
US20060272047A1 (en) 2006-11-30
US7534614B2 (en) 2009-05-19
US7001739B2 (en) 2006-02-21
EP1419253B1 (en) 2007-11-28
US20060269955A1 (en) 2006-11-30
US20030099984A1 (en) 2003-05-29
US7777096B2 (en) 2010-08-17
US20070067880A1 (en) 2007-03-22
WO2003018809A2 (en) 2003-03-06
EP1419253A2 (en) 2004-05-19
US7632936B2 (en) 2009-12-15
US20060272052A1 (en) 2006-11-30
US7632937B2 (en) 2009-12-15
WO2003018809A3 (en) 2003-11-27
US20120278946A1 (en) 2012-11-01
US20060286607A1 (en) 2006-12-21
US20060275830A1 (en) 2006-12-07
DE60223815D1 (de) 2008-01-10
US20060090217A1 (en) 2006-04-27

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements