BR0200786A - Genotyping kit for diagnosis of human papillomavirus (hpv) infection - Google Patents
Genotyping kit for diagnosis of human papillomavirus (hpv) infectionInfo
- Publication number
- BR0200786A BR0200786A BR0200786A BR0200786A BR0200786A BR 0200786 A BR0200786 A BR 0200786A BR 0200786 A BR0200786 A BR 0200786A BR 0200786 A BR0200786 A BR 0200786A BR 0200786 A BR0200786 A BR 0200786A
- Authority
- BR
- Brazil
- Prior art keywords
- dna
- hpv
- genotyping
- infection
- human papillomavirus
- Prior art date
Links
Abstract
"CONJUNTO DE GENOTIPAGEM PARA DIAGNóSTICO DE INFECçãO PELO PAPILOMAVìRUS HUMANO (HPV)". Onde um conjunto de genotipagem para diagnosticar pacientes infectadas com papilomavírus humano (HPV), e um método para diagnóstico de infecção por HPV pela genotipagem de amostras de DNA isoladas de pacientes formam o referido conjunto; dito conjunto de genotipagem compreendendo um fragmento de DNA com sondas que possuem seq³ências de nucleotídeos complementares ao DNA do HPV, primers para amplificação do DNA de uma amostra por PCR, e meios para marcar a amostra de DNA hibridizado com o fragmento de DNA; um método para diagnóstico de infecção por HPV compreendendo as etapas de amplificação do DNA obtido de uma amostra usando os primers do conjunto, aplicação do DNA amplificado no fragmento de DNA e hibridização do DNA amplificado com as sondas do fragmento de DNA, e detecção do DNA ligado à superfície do fragmento de DNA marcando o DNA hibridizado; o conjunto de genotipagem podendo ser praticamente aplicado ao diagnóstico precoce, prevenção e tratamento de câncer cervical, uma vez que o referido conjunto pode diagnosticar facilmente a infecção por HPV, e pode determinar exatamente o genótipo do HPV."GENOTYPING SET FOR DIAGNOSIS OF INFECTION BY HUMAN PAPILLOMAVIRUS (HPV)". Where a set of genotyping to diagnose patients infected with human papillomavirus (HPV), and a method for diagnosing HPV infection by genotyping DNA samples isolated from patients form the said set; said genotyping set comprising a DNA fragment with probes having nucleotide sequences complementary to HPV DNA, primers for amplifying the DNA of a sample by PCR, and means for labeling the DNA sample hybridized to the DNA fragment; a method for diagnosing HPV infection comprising the steps of amplifying the DNA obtained from a sample using the primers in the set, applying the amplified DNA to the DNA fragment and hybridizing the amplified DNA with the DNA fragment probes, and detecting the DNA attached to the surface of the DNA fragment marking the hybridized DNA; the genotyping set can practically be applied to early diagnosis, prevention and treatment of cervical cancer, since the set can easily diagnose HPV infection, and can exactly determine the HPV genotype.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0200786A BR0200786A (en) | 2002-03-14 | 2002-03-14 | Genotyping kit for diagnosis of human papillomavirus (hpv) infection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0200786A BR0200786A (en) | 2002-03-14 | 2002-03-14 | Genotyping kit for diagnosis of human papillomavirus (hpv) infection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0200786A true BR0200786A (en) | 2002-11-19 |
Family
ID=3948314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0200786A BR0200786A (en) | 2002-03-14 | 2002-03-14 | Genotyping kit for diagnosis of human papillomavirus (hpv) infection |
Country Status (1)
| Country | Link |
|---|---|
| BR (1) | BR0200786A (en) |
-
2002
- 2002-03-14 BR BR0200786A patent/BR0200786A/en not_active Application Discontinuation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired |