BR0110987A - Stable strains of xylose and arabinose fermenting zymomonas mobilis, transposon for insertion, stable of alien genes within a bacterial genome, plasmid vector containing said transposon, bacteria transformed with said plasmid, vector pzb1862-idhl-ara atcc with ascension number pta-1798, transposable element mini-tn5-tal / tkt-xilaxilb atcc with ascension number pta-1795, bacterium strain g8 atcc with ascension number pta-1796, bacterium strain c25 atcc with ascension number pta-1799, ax atcc bacterial strain with ascension number pta-1797, reaction mixture comprising fermentable medium - Google Patents

Stable strains of xylose and arabinose fermenting zymomonas mobilis, transposon for insertion, stable of alien genes within a bacterial genome, plasmid vector containing said transposon, bacteria transformed with said plasmid, vector pzb1862-idhl-ara atcc with ascension number pta-1798, transposable element mini-tn5-tal / tkt-xilaxilb atcc with ascension number pta-1795, bacterium strain g8 atcc with ascension number pta-1796, bacterium strain c25 atcc with ascension number pta-1799, ax atcc bacterial strain with ascension number pta-1797, reaction mixture comprising fermentable medium

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Publication number
BR0110987A
BR0110987A BR0110987-1A BR0110987A BR0110987A BR 0110987 A BR0110987 A BR 0110987A BR 0110987 A BR0110987 A BR 0110987A BR 0110987 A BR0110987 A BR 0110987A
Authority
BR
Brazil
Prior art keywords
atcc
number pta
ascension number
transposon
ascension
Prior art date
Application number
BR0110987-1A
Other languages
Portuguese (pt)
Other versions
BR0110987B1 (en
Inventor
Min Zhang
Yat-Chen Chou
Original Assignee
Midwest Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/565,233 external-priority patent/US7354755B2/en
Priority claimed from CA002304929A external-priority patent/CA2304929C/en
Application filed by Midwest Research Inst filed Critical Midwest Research Inst
Publication of BR0110987A publication Critical patent/BR0110987A/en
Publication of BR0110987B1 publication Critical patent/BR0110987B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"CEPAS ESTáVEIS DE ZYMIINAS MOBILIS FERMENTADORAS DE XILOSE E ARABINOSE, TRANSPOSON PARA INSERçãO ESTáVEL DE GENES ALHEIOS DENTRO DE UM GENOMA BACTERIANO, VETOR PLASMìDEO CONTENDO O REFERIDO TRANSPOSON, BACTéRIA TRANSFORMADA COM O REFERIDO PLASMìDEO, VETOR PZB1862-IDHL-ARA ATCC COM NúMERO DE ASCENçãO PTA-1798, ELEMENTO TRANSPONìVEL MINI-TN5-TAL/TKT-AXILAXILB ATCC COM NúMERO DE ASCENçãO PTA-1795, CEPA DE BACTéRIA G8 ATCC COM NúMERO DE ASCENCçãO PTA-1796, CEPA DE BACTéRIA C25 ATCC COM NúMERO DE ASCENçãO PTA-1799, CEPA DE BACTéRIA AX ATCC COM NúMERO DE ASCENçãO PTA-1797, MISTURA DE REAçãO COMPREENDENDO MEIO FERMENTáVEL". A presente invenção inclui de forma breve um transposon para a inserção estável de genes alheios dentro de um genoma bacteriano, compreendendo pelo menos um operon apresentando estruturas de gene codificando enzimas selecionadas do grupo composto por xilAxilB, araBAD e tal/tkt, e pelo menos um promotor para a expressão das estruturas de gene na bactéria, um par de seq³ências de inserção invertidas, com os operons contidos dentro das seq³ências de inserção, e um gene de transposase localizado no lado das seq³ências de inserção. Apresenta-se ainda um vetor lançador plamídeo para a transformação de genes alheios dentro de um genoma bacteriano, compreendendo pelo menos um operon apresentando estruturas de gene codificando enzimas selecionadas do grupo composto por xilAxilB, araBAD e tal/tkt, pelo menos um promotor para expressão das estruturas de gene na bactéria, e pelo menos dois fragmento de DNAs apresentando homologia com um gene no genoma da bactéria a ser trasformada. O transposon e o vetor lançador são úteis para a construção de cepas Zymomonas mobilis significativamente diferentes de acordo com a presente invenção que são úteis para a conversão dos açúcares de pentose derivados de celulose em combustíveis e produtos químicos utilizando tecnologia de fermentação tradicional porque são estáveis para expressão em um meio não seletivo."ZYMYMES MOBILIS FERMENTING ZERO AND ARABINOSIS STORAGE CODES, TRANSPOSON FOR STABLE INSERTION OF ALIEN GENES WITHIN A BACTERIAL GENOME, PLASMID VECTOR CONTAINING THE TRANSPOSMED-PERMED BETTER TRANSFORMED WITH THE TRANSFORMED-PERMITORED PLASTER PTA-1798, TRANSPONABLE ELEMENT MINI-TN5-TAL / TKT-AXILAXILB ATCC WITH ASCENTION NUMBER PTA-1795, G8 ATCC BACTERIA CUP WITH PTA-1796 ASCENTION NUMBER, C25 ATC-C99 BUTTER CUP WITH N99 AX ATCC BACTERIA CEPA WITH RANGE NUMBER PTA-1797, REACTION MIX UNDERSTANDING A FERTILIZABLE MEDIA ". The present invention briefly includes a transposon for the stable insertion of foreign genes within a bacterial genome, comprising at least one operon having gene structures encoding enzymes selected from the group consisting of xylAxilB, araBAD and tal / tkt, and at least one. promoter for the expression of gene structures in the bacterium, a pair of inverted insertion sequences, with operons contained within the insertion sequences, and a transposase gene located on the side of the insertion sequences. Also provided is a plasmid launcher vector for transforming foreign genes into a bacterial genome, comprising at least one operon having gene structures encoding enzymes selected from the group consisting of xylAxilB, araBAD and tal / tkt, at least one promoter for expression. of the gene structures in the bacterium, and at least two DNA fragments showing homology to a gene in the genome of the bacterium to be transformed. The transposon and the launcher vector are useful for constructing significantly different Zymomonas mobilis strains according to the present invention which are useful for converting cellulose derived pentose sugars into fuels and chemicals using traditional fermentation technology because they are stable for expression in a non-selective medium.

BRPI0110987-1A 2000-05-01 2001-04-06 Transposon for stable insertion of foreign genes into a bacterial genome, plasmid vector, bacterium, and reaction mixture comprising a fermentable medium. BR0110987B1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/565,233 US7354755B2 (en) 2000-05-01 2000-05-01 Stable zymomonas mobilis xylose and arabinose fermenting strains
US09/565,233 2000-05-01
CA002304929A CA2304929C (en) 2000-05-02 2000-05-02 Stable zymomonas mobilis xylose and arabinose fermenting strains
CA2,304,929 2000-05-02
PCT/US2001/011334 WO2001083786A2 (en) 2000-05-01 2001-04-06 Stable zymomonas mobilis xylose and arabinose fermenting strains

Publications (2)

Publication Number Publication Date
BR0110987A true BR0110987A (en) 2006-02-07
BR0110987B1 BR0110987B1 (en) 2014-07-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0110987-1A BR0110987B1 (en) 2000-05-01 2001-04-06 Transposon for stable insertion of foreign genes into a bacterial genome, plasmid vector, bacterium, and reaction mixture comprising a fermentable medium.

Country Status (5)

Country Link
EP (1) EP1278876A2 (en)
JP (1) JP4510355B2 (en)
AU (2) AU2001249926B2 (en)
BR (1) BR0110987B1 (en)
WO (1) WO2001083786A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003299464B2 (en) * 2000-05-01 2007-09-06 Alliance For Sustainable Energy, Llc Zymomonas pentose-sugar fermentating strains and uses thereof
US7223575B2 (en) * 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US7374939B1 (en) * 2000-05-01 2008-05-20 Midwest Research Institute Method of inactivation of an end product of energy metabolism in Zymomonas mobilis
AU2003261096B2 (en) * 2002-06-26 2008-10-02 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Gene regulation in transgenic animals using a transposon-based vector
CN100374570C (en) * 2004-07-07 2008-03-12 新疆农业科学院微生物应用研究所 Electric conversion method of making shuttle plasmid guide into yellow brevibacterium
EP1752532A1 (en) 2005-08-09 2007-02-14 Helmholtz-Zentrum für Infektionsforschung GmbH Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms
US7741119B2 (en) * 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
DE102007048134A1 (en) * 2007-10-05 2009-04-09 Evocatal Gmbh Cloning, integration and expression of a gene cluster comprises using gene cassettes comprising sequences that mediate the transfer, integration and expression of a flanked nucleic acid
ATE506447T1 (en) * 2007-10-30 2011-05-15 Du Pont METHOD FOR PRODUCING ETAHNOL IN A MEDIUM CONTAINING XYLOSE USING A RECOMBINANT ZYMOMONAS STRAIN WITH REDUCED HIMA EXPRESSION
PL2209899T3 (en) * 2007-10-30 2012-09-28 Du Pont Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate
US7998722B2 (en) * 2008-03-27 2011-08-16 E. I. Du Pont De Nemours And Company Zymomonas with improved xylose utilization
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
US20130157332A1 (en) * 2011-12-20 2013-06-20 E I Du Pont De Nemours And Company Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843760A (en) * 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
IN191596B (en) * 1996-05-06 2003-12-06 Purdue Research Foundation
US7374939B1 (en) * 2000-05-01 2008-05-20 Midwest Research Institute Method of inactivation of an end product of energy metabolism in Zymomonas mobilis

Also Published As

Publication number Publication date
JP4510355B2 (en) 2010-07-21
WO2001083786A2 (en) 2001-11-08
JP2004512819A (en) 2004-04-30
WO2001083786B1 (en) 2002-07-18
AU2001249926B2 (en) 2006-07-20
AU4992601A (en) 2001-11-12
BR0110987B1 (en) 2014-07-15
EP1278876A2 (en) 2003-01-29
WO2001083786A3 (en) 2002-06-20

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