BR0011107A - Differential and selective culture medium for the yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus, use of it, and process of detection of yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus - Google Patents

Differential and selective culture medium for the yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus, use of it, and process of detection of yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus

Info

Publication number
BR0011107A
BR0011107A BR0011107-4A BR0011107A BR0011107A BR 0011107 A BR0011107 A BR 0011107A BR 0011107 A BR0011107 A BR 0011107A BR 0011107 A BR0011107 A BR 0011107A
Authority
BR
Brazil
Prior art keywords
zygosaccharomyces
yeasts
bisporus
bailii
detection
Prior art date
Application number
BR0011107-4A
Other languages
Portuguese (pt)
Inventor
Cecilia Leao
Manuela Corte-Real
Dorit Schuller
Original Assignee
Univ Do Minho E Stab Vida Inve
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Do Minho E Stab Vida Inve filed Critical Univ Do Minho E Stab Vida Inve
Publication of BR0011107A publication Critical patent/BR0011107A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MEIO DE CULTURA DIFERENCIAL E SELETIVO PARA AS LEVEDURAS ZYGOSACCHAROMYCES BAILII E ZYGOSACCHAROMYCES BISPORUS, UTILIZAçãO DO MESMO, E, PROCESSO DE DETECçãO DE LEVEDURAS ZYGOSACCHAROMYCES BAILII E ZYGOSACCHAROMYCES BISPORUS". A invenção refere-se a um meio de cultura diferencial e seletivo para detectar leveduras das espécies Zygosaccharomyces bailii e Zygosaccharomyces bisporus, que permite reduzir drasticamente o tempo e trabalho envolvidos na identificação convencional destas espécies. De acordo com a presente invenção, a detecção de Zygosaccharomyces bailii e Zygosaccharomyces bisporus resume-se a um único teste que exige apenas a elaboração de um meio de cultura sólido ou líquido e a sua inoculação. Este meio de cultura é constituído por um meio mineral base, suplementado com oligoelementos e vitaminas, por glucose e ácido fórmico como únicas fontes de carbono e energia, e por um indicador ácido-base. O indicador ácido-base, em particular verde de bromocresol, no meio confere-lhe uma coloração verde que é convertida a azul por estas leveduras. Adicionalmente, as colónias apresentam uma coloração azul que é uma característica específica destas espécies e pode ser visível no meio entre as 48 e 96 horas de incubação, consoante a metodologia de inoculação utilizada. A invenção pode-se aplicar quer a estirpes de leveduras previamente isoladas e purificadas, quer a suspensões celulares de populações mistas de leveduras, para além de Zygosaccharomyces bailii e Zygosaccharomyces bisporus, para detecção destas espécies na indústria alimentar, nomeadamente em vinhos e outras bebidas. O meio poderá ainda ser integrado em galerias de testes de identificação de leveduras."DIFFERENTIAL AND SELECTIVE CULTURE MEDIA FOR YEASTS ZYGOSACCHAROMYCES BAILII AND ZYGOSACCHAROMYCES BISPORUS, USE OF THE SAME, AND, PROCESS OF DETECTION OF YEASTS ZYGOSACCHAROMYCES ZARZYCHOSOMYCHESEYEZ BACS. The invention relates to a differential and selective culture medium to detect yeasts of the species Zygosaccharomyces bailii and Zygosaccharomyces bisporus, which allows to drastically reduce the time and work involved in the conventional identification of these species. According to the present invention, the detection of Zygosaccharomyces bailii and Zygosaccharomyces bisporus is limited to a single test that requires only the elaboration of a solid or liquid culture medium and its inoculation. This culture medium consists of a base mineral medium, supplemented with trace elements and vitamins, glucose and formic acid as the sole sources of carbon and energy, and an acid-base indicator. The acid-base indicator, in particular bromocresol green, in the middle gives it a green color that is converted to blue by these yeasts. In addition, the colonies have a blue color that is a specific characteristic of these species and can be visible in the medium between 48 and 96 hours of incubation, depending on the inoculation methodology used. The invention can be applied either to strains of previously isolated and purified yeasts, or to cell suspensions of mixed yeast populations, in addition to Zygosaccharomyces bailii and Zygosaccharomyces bisporus, for the detection of these species in the food industry, namely in wines and other drinks. The medium may also be integrated into yeast identification test galleries.

BR0011107-4A 1999-05-31 2000-05-31 Differential and selective culture medium for the yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus, use of it, and process of detection of yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus BR0011107A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT10230599A PT102305B (en) 1999-05-31 1999-05-31 MEANS OF CULTURE FOR THE DETECTION OF YEAST ZYGOSACCHAROMYCES BAILII AND ZYGOSACCHAROMYCES BISPORUS
PCT/PT2000/000004 WO2000073494A1 (en) 1999-05-31 2000-05-31 Culture medium for the detection of zygosaccharomyces

Publications (1)

Publication Number Publication Date
BR0011107A true BR0011107A (en) 2002-03-05

Family

ID=20085853

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0011107-4A BR0011107A (en) 1999-05-31 2000-05-31 Differential and selective culture medium for the yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus, use of it, and process of detection of yeasts zygosaccharomyces bailii and zygosaccharomyces bisporus

Country Status (10)

Country Link
EP (1) EP1185685A1 (en)
JP (1) JP2003501047A (en)
CN (1) CN1367842A (en)
AU (1) AU5116300A (en)
BR (1) BR0011107A (en)
CA (1) CA2375111A1 (en)
NZ (1) NZ515657A (en)
PT (1) PT102305B (en)
WO (1) WO2000073494A1 (en)
ZA (1) ZA200109748B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5850741B2 (en) * 2011-12-27 2016-02-03 株式会社明治 Yeast and mold detector
EP2889369B1 (en) 2012-08-24 2020-03-25 Yamaguchi University Yeast culture medium
HUP1300186A2 (en) 2013-03-29 2014-10-28 Univ Szegedi Selective chromogen culture-medium

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882882A (en) * 1995-04-12 1999-03-16 Biolog, Inc. Gel matrix with redox purple for testing and characterizing microorganisms

Also Published As

Publication number Publication date
PT102305B (en) 2002-01-30
WO2000073494A1 (en) 2000-12-07
CA2375111A1 (en) 2000-12-07
ZA200109748B (en) 2003-02-27
NZ515657A (en) 2004-01-30
CN1367842A (en) 2002-09-04
WO2000073494B1 (en) 2001-02-08
JP2003501047A (en) 2003-01-14
AU5116300A (en) 2000-12-18
EP1185685A1 (en) 2002-03-13
PT102305A (en) 2000-11-30

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