BG40818A3 - Method for spectrophotometric determining of glucose content in non- enzymic glucosinated proteins - Google Patents
Method for spectrophotometric determining of glucose content in non- enzymic glucosinated proteinsInfo
- Publication number
- BG40818A3 BG40818A3 BG064223A BG6422384A BG40818A3 BG 40818 A3 BG40818 A3 BG 40818A3 BG 064223 A BG064223 A BG 064223A BG 6422384 A BG6422384 A BG 6422384A BG 40818 A3 BG40818 A3 BG 40818A3
- Authority
- BG
- Bulgaria
- Prior art keywords
- proteins
- spectrophotometric
- glucose content
- glucosinated
- enzymic
- Prior art date
Links
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title abstract 3
- 239000008103 glucose Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 abstract 2
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 238000005903 acid hydrolysis reaction Methods 0.000 abstract 1
- 150000001299 aldehydes Chemical class 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 102000035122 glycosylated proteins Human genes 0.000 abstract 1
- 108091005608 glycosylated proteins Proteins 0.000 abstract 1
- 150000002894 organic compounds Chemical class 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 238000002798 spectrophotometry method Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Метод за спектрофотометрично определяне на глюкозното съдържание на неензимно глюкозирани протеини, характеризиращ се с това, че протеини, включени в клетки или неразтворими във вода, се разтварят с помощта на йонен или нейонен детергент, глюкозата се превръща в 5-хидроксиметилфурфурол чрез кисела хидролиза под свръхналягане, като се използва поне 1n киселина, реакционната смес се депротеинизира, 5-хидроксиметил-фурфуролът взаимодействува с органично съединение, което образува оцветено съединение с алдехида, и концетрацията на продукта се определя спектрофотометрично. Патентните претенции са формулирани в 9 точки.A method for the spectrophotometric determination of the glucose content of non-enzymatically glycosylated proteins, characterized in that cell-encapsulated or water-insoluble proteins are solubilized using an ionic or nonionic detergent, glucose is converted to 5-hydroxymethylfurfural by acid hydrolysis under overpressure , using at least 1n acid, the reaction mixture is deproteinized, the 5-hydroxymethyl-furfural reacts with an organic compound that forms a colored compound with the aldehyde, and the concentration of the product is determined spectrophotometrically. The patent claims are formulated in 9 points.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG064223A BG40818A3 (en) | 1984-02-10 | 1984-02-10 | Method for spectrophotometric determining of glucose content in non- enzymic glucosinated proteins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG064223A BG40818A3 (en) | 1984-02-10 | 1984-02-10 | Method for spectrophotometric determining of glucose content in non- enzymic glucosinated proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG40818A3 true BG40818A3 (en) | 1987-02-16 |
Family
ID=3913387
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG064223A BG40818A3 (en) | 1984-02-10 | 1984-02-10 | Method for spectrophotometric determining of glucose content in non- enzymic glucosinated proteins |
Country Status (1)
| Country | Link |
|---|---|
| BG (1) | BG40818A3 (en) |
-
1984
- 1984-02-10 BG BG064223A patent/BG40818A3/en unknown
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