BG1473U1 - Biologically active product for stock-breeding - Google Patents

Abstract

The useful model relates to a biologically active product, which can be applied as a food additive in the stock-breeding. Basically, it is a composition including whey bio concentrate, dry fermented fat-free milk, lactic acid bacteria, lactose fermenting yeasts, Propionni bacteria and vitamins.

Description

Technical field

The utility model refers to a biologically active lactic acid product useful as a food additive in animal husbandry.

BACKGROUND OF THE INVENTION

Whey, although a waste product from the dairy industry, is known to have valuable biological and nutritional properties because of its soluble milk proteins, milk sugar, minerals, trace elements and vitamins. Based on these properties, according to patent application KR 20080088697, whey obtained as a by-product of cheese production is added to the poultry feed in an amount of 0.3 to 10 wt. %. However, when administered alone, this product is insufficient for the proper nutrition of animals.

Probiotics are also known to be used in animal husbandry for overcoming dysbiosis, reduced disease resistance and drug intolerance caused by the use of large quantities of antibiotics and chemical drugs over a long period of time. To overcome the problem, according to patent CN 101368165, it is proposed to feed 3 to 10 parts of Lactobacillus acidophilus, 2 to 6 parts of yeast, 1 to 8 parts of Bacillus subtilis, 3 to 8 parts of Bifidobacterium longum, 2 to 5 parts in animal feed Bacillus licheniformis and 1 to 4 parts of Aspergillus oryzae. This document; however, it does not provide information on the one hand that probiotic organisms are provided with an environment suitable for their development, and on the other hand, there is no data on their survival and the impact of the combination on a specific species of farm animals and on specific adverse conditions of their organism.

The object of PM BG 1181 is a whey-based biologically active product enriched with valuable metabolites from the vital activity of selected strains of lactic acid bacteria in a suitable combination with lactose-fermenting yeast, herbal extracts and sucrose. The microbial association, where the microorganisms, in particular Lactobacterium delbrueckii ssp. bulgaricus, Streptococcus thermophilus Saccharomyces Iactis, 4: 2: 1 ratio was created specifically to be able to survive in the presence of the essential oils and bitter substances contained in the herbal extracts included in the biologically active product. It is intended for the rational nutrition of humans.

The technical nature of the utility model

The task of the utility model is to create a biologically active animal product, including whey enriched with valuable metabolites from the life activity of a suitable combination of selected strains of lactic acid bacteria, lactose-fermenting yeast and propionic bacteria, dry fermented and non-fermented non-fat milling.

In particular, the biologically active product according to the utility model is a composition that includes the following components in the following quantitative ratio (wt.%): Whey bioconcentrate in an amount of 40.00 to 42.00;

dry fermented skim milk from 56.00 to 58.5;

culture of lactic acid bacteria, lactose-fermenting yeast and propionic bacteria from 0.8 to 1.5;

vitamins (B1, B2, PP and folic acid in a ratio of 1: 2: 1: 0.5) from 0.4 to 0.5.

The whey bioconcentrate, according to the utility model, is obtained after fermentation of whey from the production of white brine cheese with starter culture, which is a combination of three pre-developed cultures of microorganisms taken at a ratio of 2: 2: 1, namely:

first crop - Lactobacillus delbrueckii ssp. bulgaricus NBMCC No. 8356 and Str. salivarius thermophilus NBMCC No. 8357 in a 2: 2 ratio;

second culture - lactose-fermenting yeast of Saccharomyces Lactis type and third culture - microorganisms of the species Propionibacterium shermanii.

The fermentation of the feedstock is considered complete upon reaching the active acid-

1473 Ul of 72-75 ° T, pH 5.1-5.2, followed by freeze-drying in the usual manner.

For the preparation of dry fermented skim milk, cow's milk is used, pasteurized at 96-98 ° C with a holding time of 20 s and cooled to 38-43 ° C. Thus treated, the milk is fermented with 1% yeast including Lactobacterium delbrueckii ssp. bulgaricus NBPMCC No. 8356, Lactobacillus acidophilus NBPMCC No. 8358, Streptococcus thermophilus NBPMCC No. 8357, and Lactococcus lactis NBPMCC No. 8482. The fermentation of the raw material is considered complete upon reaching a pH of 72.1, 5.5% acidity, 75.1-5.5 acidity. after which the product is subjected to freeze drying in the usual manner.

According to one embodiment of the utility model, for the purposes of use as a feed additive for poultry, the biologically active product is applied in an amount of 0.45 to 0.5% by feed.

According to a second embodiment of the utility model, for the purposes of use as a rabbit nutritional supplement, the biologically active product is incorporated into the drinking water in an amount of from 1 00 to 0.12%.

According to a third embodiment of the utility model, for the purposes of use as a dietary supplement for calves, the biologically active product is applied in an amount of 0.9 to 1.00% relative to the individual daily ration of a mammal.

The advantage of the biologically active product, according to the utility model, is that the combination of ingredients, as well as the presence of the microorganisms involved and, in particular, the products of their exchange, contributes to the content in the final product of:

Proteins 29.29% including: casein 18.2%, soluble protein 11.29% containing lactoalbumin 6.0%; lactoglobulin 3.0%; (F-globulin 1.8%; pseudoglobulin 1.2%), proteosopeptone 1.8-2.0%;

lactose 52.14 (%);

minerals 8.76 (g / kg), including: calcium 1.18; magnesium 0.12; potassium 1,40; sodium 0,58; phosphorus 0.95; sulfur 0.34;

acids (%): lactic 2.75; propionic 3,10;

vitamins - a total amount of 119.92 g / kg, including: B1 10.13; B2 31.74; RR 50.82; folic acid 27.23;

free amino acids - total 479.65 mg%, including: lysine 38.63; histidine 11.68; isoleucine 23.90; leucine 41.95; methionine 13.45; arginine 1.82; aspartic acid 29,29; threonine 19.23; serine 31.19; glutamic acid 59.61; Proline 44.65; glycine 17.41; alanine 17.86; cystine 42.90; valine 39.75; tyrosine 16.49; phenylalanine 1.85; tryptophan 27.99.

In addition, the biologically active product, according to the utility model, regulates the animal's digestive system; ensures balance of biologically active substances in organisms; increases the physiological parameters, and therefore - has a beneficial effect on the important characteristics of animal husbandry - live weight gain, laying capacity of hens, egg quality, etc.

Figure 1 illustrates the intensity of laying eggs of laying hens after feeding with the biologically active product according to the utility model. Periods of study, 15 days each, and abscissa -% load bearing capacity are plotted on the ordinate.

The utility model is illustrated by the following examples, without limiting its scope:

Example 1. Preparation of starting components

For the preparation of whey biologically active concentrate, whey from the production of white brined cheese is concentrated to a dry matter of 30-35% and subjected to fermentation with the following types of pre-grown cultures: first culture Lactobacillus delbrueckii ssp. Bulgaricus NBPCC No. 8356 and Str. salivarius thermophilus NBPMCC No. 8357 in a 2: 2 ratio; second crop - lactose-fermenting yeast of Saccharomyces Lactis type and third crop - selected strains of Propionibacterium shermanii. The starter culture for obtaining whey bioconcentrate is a combination of the three cultures in a 2: 2: 1 ratio. The fermentation of the feedstock is considered complete upon reaching an active acidity of 72-75 ° T, pH 5.1 -5.2, after which it is subjected to freeze drying in the usual way to a moisture content of 3-6%.

Cow's milk pasteurized at 96-98 ° C with a holding time of 20 s and cooled to 38-43 ° C is used to prepare the dry fermented skeletal milk. Thus treated, the milk is fermented with 1% yeast including Lactobacterium delbrueckii ssp. bulgaricus - 5 NBPCC No. 8356, Lactobacillus acidophilus NBPMCC No. 8358, Streptococcus thermophilus strain NBPMCC No. 8357 and Lactococcus lactis NBPMCC No. 8482. 5.2, and then subjected to freeze-drying in the usual manner.

Example 2. Biologically active product intended for use as a dietary supplement for poultry nutrition 15

To obtain the final product intended for poultry, 40.00 g of whey biologically active concentrate, obtained as described in Example 1 above, is mixed with 56.5 g of dry fermented milk and 0.5 g are added thereto. vitamins B1, B2, PP and folic acid. It is applied in the dry state in the amount of 5% relative to the feed.

The results of the test of the biologically active product (BAP) on chickens and laying hens according to the utility model applied to the feed at 0.5% are shown in Tables 1-4 below, reflecting, respectively, the effect on growth, egg productivity, the morphological and incubation qualities of the eggs as follows:

Table 1- Impact on growth

| GROUP Live weight at start (kg) Live weight at the end (kg) Mortality (%) i Control 4.034 ± 0.035 3,881 ± 0.043 9:18 I Experienced 3,94110,041 _ 3.959 ± 0.047 6.12

Table 2- Egg productivity

Indicators The group control Experienced Carrying capacity (number of eggs) 44.68 48.82 Laying intensity (%) 59.39 ± 1.86 66.57 ± 2.46 Average mass of eggs (e) 60.25 ± 0.95 60.54 ± 0.40 _______________________________________________________________________________________________________________________________________________________________________________I Amount of egg mass (e) 2691,97 2955.56 Feed consumption per egg (kg) 0.270 0.239 Feed consumption per 1 kg egg mass (kg) 4,476 th most common 3,961 th most common

Table 3- Morphological characteristics of eggs

Indicator PERIOD I PERIOD II PERIOD III PERIOD IV control EXPERIENCE control EXPERIENCE control EXPERIENCE control experience LSi SE LSI SE LSi SE LSi SE LSi SE LSi SE LSi SE LSi SE Format index 77.23 ± 0.90 78.13 i 0.48 77.40 ± 0.67 77.30 ± 0.56 76.47 ± 0.65 76.84 ± 0.48 77.03 ± 0.43 77.43 ± 0.29 Yolk index 45.50 ± 0.55 45.05 ± 0.49 ' 44.56 ± 1.46 44.63 ± 0.53 44.14 ± 0.43 43.41 ± 0.61 ' 44.74 ± 0 30 44.36 ± 032 Protein index 9.21 ± 0.49 ati 8.79 ± 0.29 " 7.44 ± 0.31 ' 7.47 ± 0.38 p 7.18 ± 0.29 ¹¹ 6.98 ± 0.42 ' 7.94 ± 0.24 7.75 1 0.23 Huff 82.16 ± 82,001 74.66 ± 74.66 ± 0.8 73.83 ± 1.59 73.06 ± 76.89 ± 1.05 76.58 ± units 1.91 ^{, b} 1.25 " 1.57 ' 2.11 1.13 Thickness 0.330 ± 0.335 ± 0.350 ± 0.357 ± 0,343 ± 0.341 ± 0,341 ± 0.344 ± the shell 0.005 ' 0,005 ^b 0.005 * 0.004 * 0.006 0,005 ^of 0 004 --- ---- I 0.003 I

- identical letters in the order indicate statistically significant differences at p <0,05

- LS and SE are mean + error

1473 Ul

Table 4- Egg hatching qualities

Indicators The group control experienced Number of eggs loaded 407 439 % fertility 95.58 96.81 Embryonic to the 6th day 1,23 1.59 mortality by day 18 7.12 6.83 Lz% 86.98 88.38 Lop% 91,00 91,29

Lz is the hatching of loaded eggs

Lop - hatching of fertilized eggs

Example 3. Application of the biologically active product according to the utility model as a feed additive for feeding rabbits

The rabbit biologically active product includes 42.00 g of whey biologically active concentrate obtained as described in Example 1 above, 58.0 g of fermented milk powder and 0.5 g of vitamins Bl, B2, PP and folic acid. It is applied in the dry state in the amount of 0.1% of the daily ration of drinking water.

The effect of the biologically active product (BAP), according to the utility model, was tested on the growth of White New Zealand breeds of rabbits. The test groups were formed according to the analog scheme. The results are set out in Table 5 below.

Table 5- Effect of BAP on rabbit growth

The group Date of withdrawal 19.03 30.03 15.04 12.05 31.05 16.06 experienced 1.34 1.59 1.87 2.61 3.17 3,32 control 1,25 1.45 1.76 2.40 3.10 3.26 experienced 0.96 1.37 1.91 2.19 2.80 3.01 control 0.88 1.31 1.79 2.18 2.62 2.86

Apart from the apparently higher increase due to the use of BAP as a supplement for the rational feeding of rabbits, the results of the experiment show that the mortality in the experimental group (that using BAP) is 410% lower than that in the control group (without BAP).

Example 4. Biologically active product intended for use as a dietary supplement for the rational nutrition of calves

The biologically active product intended for calves includes 42.00 g of whey biologically active concentrate prepared as described in Example 1 above, 58.5 g of dry fer40 mentee milk and 0.5 g of vitamins Bl, B2, PP and folic acid.

For the purpose of feeding calves, the biologically active product according to the utility model is dissolved in the daily ration of a mammal, namely - each calf receives on day 71 a mammal to which 7.5 g of BAP is added. In addition, the animals receive additional calf starter calf feed at will.

The effect of the biologically active product (BAP), according to the utility model, was tested on calf growth. For this purpose, each calf is weighed after a period of 10 days and, based on the results obtained, the average daily increment for the respective period is calculated. The data are summarized in Table 6 below.

Table 6- Effect of BAP on calf growth

1 »RUL9 with BAP wind. No 20.09 / kg 30.0 9 kg Nature 13.10 kg Growth / day 22.10 kfl Gain / data 2.11 kg Approx. / day 12.11 kg gain / day days 9 experience start up gain experience average day for the test period 939437 41 46 0.500 46 0 56 1.111 63 0636 72 0900 53 31 0.585 939424 46 50 0.400 56 0.462 59 0.333 72 1 182 80 0.800 53 34 0642 939434 46 53 0.700 63 0.769 65 0.222 75 0 909 81 0.600 53 35 0.560 939438 55 61 0.600 69 0615 75 0.667 65 0 909 95 1,000 53 4G 0.755 939439 44 50 0600 58 0.615 65 0.778 70 0 455 83 1.300 53 39 0.736 93944? 36 40 0.444 47 0 636 52 0.500 30 16 0533 939446 41 43 0.200 10 2 0.200 soo 46 4 52 0.560 58,4 0.492 60 0.593 64.71 0.788 72.29 0.757 43 5 28 0 587 όροι * 5 5 6 6 6 6 7 -— _Mr ----- 7 7

II group of iozhpool ---------------------------------------- 1 - Bei no 20.09 / ^ks 9 30.09 kg Hi / day 13:10 ^k January Growth / day 22.10 kg Growth / day 2.11 kg Growth / day 12.11 kg gain / day days is an experience incremental beginning experience cp.dtt.npup trial period 939436 39 41 0.200 42 0,077 49 0.778 59 0.909 63 .400 53 24 0.453 939425 62 67 0.500 79 0.923 83 0,444 th most common 92 0.818 99.5 0.750 53 38 0.708 939435 44 49 0.500 56 0.538 60 0,444 th most common 65 0.455 77 1,200 53 33 0.623 939433 44 49 0.500 57 0.615 61 0,444 th most common 68 0.636 79 1,100 53 35 0.660 939448 47 50 0.333 57 0.636 65 0.800 30 18 0.600 939445 40 44 .400 10 4 0.400 on average 48,8 53 0.420 58.5 0.423 60.6 0.489 88.2 0.691 71 0.775 42 25 0 574 number 4 4 5 5 5 5 6 6 6 6

Claims

Claims (6)

Claims 1. Whey-based biologically active livestock product, characterized in that it comprises (in wt.%): Whey bioconcentrate in an amount of 40.00 to 42.00; dry fermented skim milk from 56.00 to 58.5; culture of lactic acid bacteria, lactose-fermenting yeast and propionic bacteria from 0.8 to 1.00; vitamins (B2, B2, PP and folic acid in a ratio of 1: 2: 1: 0.5) from 0.4 to 0.5. The biologically active product according to claim 1, characterized in that the whey bioconcentrate comprises a combination of three pre-grown cultures of microorganisms in a ratio of 2: 2: 1, especially Lactobacillus delbrueckii ssp. bulgaricus NBPMCC No. 8356 and Streptococcus salivarius 45 thermophilus NBPMCC No. 8357 at a ratio of 2: 2, lactose-fermenting yeast of the Saccharomyces lactis type and microorganisms of the species Propionibacterium shermanii. Biologically active product according to Claim 50, characterized in that the dry fermented skimmed milk contains the microorganisms Lactobacterium delbrueckii ssp. bulgaricus NBPCC No. 8356, Lactobacillus acidophilus NBMCC No. 8358, Streptococcus thermophilus NBMCC No. 8357, and Lactococcus lactis NBMCC No. 8482. Biologically active product according to claims 1 to 3, characterized in that it is used as an animal feed additive in an amount of 0.45 to 0.5% by weight of the feed. Biologically active product according to claims 1 to 3, characterized in that it is used in the amount of 0.1 to 0.12% in relation to drinking water for use in rabbits. Biologically active product according to claims 1 to 3, characterized in that it is used in an amount of 0.9 to 1.00% for use in calves relative to the individual daily ration of a mammal.