BE712331A - - Google Patents

Info

Publication number
BE712331A
BE712331A BE712331DA BE712331A BE 712331 A BE712331 A BE 712331A BE 712331D A BE712331D A BE 712331DA BE 712331 A BE712331 A BE 712331A
Authority
BE
Belgium
Prior art keywords
action
cell
desc
page number
clms page
Prior art date
Application number
Other languages
French (fr)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of BE712331A publication Critical patent/BE712331A/fr

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical methods
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/08Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
    • B01J19/10Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor employing sonic or ultrasonic vibrations
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Description

  

   <Desc/Clms Page number 1> 
 



  "PROCEDE ET DISPOSITIF D'EXTRACTION EN CYCLE CONTINU OU 
DISCONTINU DES PRINCIPES CELLULAIRES DES   MATIERES   ORGANIQUES". 

 <Desc/Clms Page number 2> 

 



  I N T R O D U C T I O N 
Le présent mémoire a pour but de décrire un procédé d'extrac- tion des principes intra-cellulaires spécifiques aux matières orga-   @   .: niques qu'elle qu'en'soit l'origine, animale, minèrale ou végétale et, corollairement, les moyens mis en oeuvre pour réaliser ledit pro-   @   cédé en cycle continu. 



   La particularité fondamentale de l'invention réside dans la mise en oeuvre et la combinaison simultanée d'agents physiques et de réactions chimiques destinées à séparer le contenu intra-oellulaire de la cellule proprement dite. La matière à traiter est additionnée de produits abrasifs de granulométrie bien définie par la nature, la texture ou plus généralement l'architecture des tissus, fibres ou   .]il   complexes cellulaires de constitution. 



   A titre exemplatif, les abrasifs les plus utilisés sont le sable, le porphyre et le verre pilé sans toutefois que ces exemples - soient limitatifs. 



   L'action de ces abrasifs, dans la masse mise en mouvement est complétée par l'application de pressions mécaniques agissant sur l'ensemble de-cette même masse soumise simultanément à l'action d'un générateur d'ultra-sons. 



   La résultante de cette triple action physique, mouvement, .pression et ultra-sons se traduit par une pénétration intime des substances abrasives dans les espaces inter-cellulaires dont elles' détruisent la structure et la cohésion tout en préparant la pénétra- tion des agents chimiques destinée à l'attaque de la paroi cellulaire - proprement dite.. 

 <Desc/Clms Page number 3> 

      



   Cette attaque est réalisée par l'addition de solutions éleotrolytiques convenablement choisies ( sels de Nickel, Cobalt ou Cuivre par exemple) dont le rôle est de modifier la perméabilité initiale de la membrane de la cellule et-de faciliter le passage d'un courgnt électrique de forme et d'amplitude tels que la paroi cellulaire éclate au passage de celui-ci et se libère de son contenu.! 
Après filtration, ce contenu est recueilli par électro-dia- lyse au travers d'une membrane de composition ( cellophane, cellulose!      ou porcelaine selon le cas) de porosité bien déterminée, 
Les produits tant anodique que cathodique sont recueillis ensemble ou séparément.

   Au cours de l'électrodialyse on neutralise   @   l'alcalinité cathodique par admission d'un courant d'acide carboni- que gazeux à la cellule cathodique, 
Les opérations décrites ci-dessus sont réalisées simultané- ! ment en cycle continu de la manière suivante ( voir figure 1). 



   Un tambour rotatif T, est muni de deux électrodes concen- triques El et E2 réunies à un générateur de tension à fréquence ultra-sonique G et reçoit la   matièro à   traiter préalablement addi- tionnée des substances abrasives ad'hoc et de la solution électro- lytique comme indiquée ci-dessus. 



   Le tambour tourne à l'intérieur d'une enoeinte hermétique et thermostatée H au sein de laquelle on établit la pression P tandis que l'on lance le courant entre El et E2. 



   Après cette première opération, la matière est envoyée dans le filtre sous pression F, puis passe au travers d'une série de membranes semi perméables Ml à M4 puis soumise à   l'éleotrodialyae   entre les électrodes E3 et E4 tandis qu'une bonbonne B de gaz carbo- nique (C02) neutralise l'alcalinité de l'extrait en E4. 

 <Desc/Clms Page number 4> 

 



   Ayant décrit la nature de   l'invention,   les principes mis en oeuvre et la façon de la reproduire nous posons les revendications suivantes. 



   REVENDICATIONS 
1. Procède d'extraction des principes intra-cellulaires caractérisé par le fait que le contenu de la cellule est   libéré   en provoquant l'éclatement de sa paroi par l'action simultanée, alternée ou séparée de a) une substance abrasive. b) une pression mécanique appliquée à la matière* c) l'action des ultra-sons. d) l'action   d'un   ou de plusieurs électrolytes. c) l'action d'un courant électrique   comme, décrit   dans le présent ¯¯¯ mémoire. 



   2' Comme au primo   ci-dessus,   le contenu cellulaire étant recueilli par électrodialyse avec membrane de composition. 



     3* Comma   au secundo ci-dessus, avec introduction d'un courant d'acide .'carbonique gazeux à la cellule cathodique d'électrodialyse pour en neutraliser l'alcalinité. 

**ATTENTION** fin du champ DESC peut contenir debut de CLMS **.



   <Desc / Clms Page number 1>
 



  "CONTINUOUS CYCLE EXTRACTION PROCESS AND DEVICE OR
DISCONTINUED FROM CELLULAR PRINCIPLES OF ORGANIC MATTERS ".

 <Desc / Clms Page number 2>

 



  I N T R O D U C T I O N
The purpose of this report is to describe a process for extracting the intracellular principles specific to organic materials, regardless of their origin, animal, mineral or plant and, corollary, the means implemented to carry out said process in a continuous cycle.



   The fundamental peculiarity of the invention resides in the simultaneous use and combination of physical agents and chemical reactions intended to separate the intra-cellular contents from the cell proper. The material to be treated is supplemented with abrasive products of grain size well defined by the nature, the texture or more generally the architecture of the fabrics, fibers or.] He cellular complexes of constitution.



   By way of example, the most widely used abrasives are sand, porphyry and crushed glass, without however these examples being limiting.



   The action of these abrasives in the mass set in motion is completed by the application of mechanical pressures acting on the whole of this same mass subjected simultaneously to the action of an ultrasound generator.



   The result of this triple physical action, movement, pressure and ultrasound results in an intimate penetration of abrasive substances into the inter-cellular spaces of which they destroy the structure and cohesion while preparing the penetration of chemical agents. intended for attacking the cell wall - proper.

 <Desc / Clms Page number 3>

      



   This attack is carried out by the addition of eleotrolytic solutions suitably chosen (nickel, cobalt or copper salts for example) whose role is to modify the initial permeability of the cell membrane and to facilitate the passage of an electric current. of shape and amplitude such that the cell wall bursts as it passes and is released from its contents.!
After filtration, this content is collected by electro-dialysis through a membrane of composition (cellophane, cellulose! Or porcelain as the case may be) of well-determined porosity,
Both anode and cathode products are collected together or separately.

   During electrodialysis, the cathodic alkalinity is neutralized by admitting a stream of carbonic acid gas to the cathode cell,
The operations described above are carried out simultaneously! continuously cycle as follows (see figure 1).



   A rotating drum T, is provided with two concentrated electrodes E1 and E2 joined to an ultrasonic frequency voltage generator G and receives the material to be treated previously added ad hoc abrasive substances and electro-electro solution. lytic as indicated above.



   The drum rotates inside a hermetic and thermostatically controlled enclosure H within which the pressure P is established while the current is launched between El and E2.



   After this first operation, the material is sent to the filter under pressure F, then passes through a series of semi-permeable membranes M1 to M4 then subjected to the eleotrodialyae between the electrodes E3 and E4 while a cylinder B of carbon dioxide (C02) neutralizes the alkalinity of the extract to E4.

 <Desc / Clms Page number 4>

 



   Having described the nature of the invention, the principles used and the way of reproducing it, we make the following claims.



   CLAIMS
1. Extraction process for intracellular principles characterized by the fact that the contents of the cell are released by causing the bursting of its wall by the simultaneous, alternating or separate action of a) an abrasive substance. b) mechanical pressure applied to matter * c) the action of ultrasound. d) the action of one or more electrolytes. c) the action of an electric current as, described in the present ¯¯¯ brief.



   2 'As in the first step above, the cell content being collected by electrodialysis with composition membrane.



     3 * Comma secondly above, with introduction of a stream of gaseous carbonic acid to the electrodialysis cathode cell to neutralize the alkalinity.

** ATTENTION ** end of DESC field can contain start of CLMS **.

 

Claims (1)

4 Comme au secundo ci-dessus les -produits anodique et cathodique --étant receuillis séparément ou en mélange. **ATTENTION** fin du champ CLMS peut contenir debut de DESC **. 4 As in the second above, the anode and cathode products - being collected separately or as a mixture. ** CAUTION ** end of field CLMS may contain start of DESC **.
BE712331D 1968-03-18 1968-03-18 BE712331A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BE712331 1968-03-18

Publications (1)

Publication Number Publication Date
BE712331A true BE712331A (en) 1968-07-31

Family

ID=3852576

Family Applications (1)

Application Number Title Priority Date Filing Date
BE712331D BE712331A (en) 1968-03-18 1968-03-18

Country Status (1)

Country Link
BE (1) BE712331A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0065246A2 (en) * 1981-05-14 1982-11-24 Elkawi Ag Process for producing anabolic, respiration promoting, low-molecular-weight-active substances for prophylactic, therapeutic and cell and tissue culture technique purposes
WO1988002777A1 (en) * 1986-10-10 1988-04-21 Electropore Inc. Process for extracting cell contents
EP0288618A1 (en) * 1986-03-20 1988-11-02 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
FR2618793A1 (en) * 1987-07-10 1989-02-03 Berlin Kosmetik Veb METHOD AND DEVICE FOR OBTAINING BIO-ACTIVE SUSPENSIONS

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0065246A2 (en) * 1981-05-14 1982-11-24 Elkawi Ag Process for producing anabolic, respiration promoting, low-molecular-weight-active substances for prophylactic, therapeutic and cell and tissue culture technique purposes
EP0065246A3 (en) * 1981-05-14 1983-07-06 Elkawi Ag Process for producing anabolic, respiration promoting, low-molecular-weight-active substances for prophylactic, therapeutic and cell and tissue culture technique purposes
EP0288618A1 (en) * 1986-03-20 1988-11-02 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
WO1988002777A1 (en) * 1986-10-10 1988-04-21 Electropore Inc. Process for extracting cell contents
FR2618793A1 (en) * 1987-07-10 1989-02-03 Berlin Kosmetik Veb METHOD AND DEVICE FOR OBTAINING BIO-ACTIVE SUSPENSIONS

Similar Documents

Publication Publication Date Title
Nguyen et al. Hydrophobic effect on gas hydrate formation in the presence of additives
BE712331A (en)
CN104231303B (en) Preparation method and applications of molecular imprinting composite membrane
CN101898121B (en) Filter medium for removing beryllium in drinking water and preparation method thereof
JPS59130508A (en) Slurry filtration
KR101446752B1 (en) Filter cloth-fixing type filter press
CN206597488U (en) A kind of micro-filtration or milipore filter regenerative agent preparation facilities
Gambihler et al. Influence of dissolved and free gases on iodine release and cell killing by shock waves in vitro
WO2012132007A1 (en) Process and apparatus for producing regenerated tobacco material
JPS63303806A (en) Purification of diamond
JP5629805B2 (en) Nanobubble manufacturing method and nanobubble manufacturing apparatus
AU2021314895A1 (en) Method for the bonding, transport, reaction activation, conversion, storage and release of water-soluble gases
AR098036A2 (en) PREPARATION OF A PARTICULATED PRODUCT OF TETRAHIDRO-3,5-DIMETIL-1,3,5-TIADIAZIN-2-TIONA
RU2542202C1 (en) Method of extracting admixture elements from mineral raw material
TW201716371A (en) System and method for continuous manufacturing of sodium sarcosinate
Slocombe et al. The development of a standard method for rapid ecdysis of infective Haemonchus contortus cayugensis larvae
Hwang et al. Properties and Applications of Cryptand‐22 Surfactant for Ion Transport and Ion Extraction
SU1346111A1 (en) Method of producing tea concentrate
CN109930194B (en) Sm (OH) CO (carbon monoxide) hydroxyl-based samarium carbonate grown at high temperature and high pressure3Method for single crystal
FR3128948A3 (en) Manual bottle holder or two rings associated or combined with energy impressions of quantum bio-resonance and energizing magnets and the integration of a magnetic tape encoded by a quantum frequency.
Ungaro et al. Calixarene-based cation receptors and carriers
SU149709A1 (en) A device for tightening and cutting packaging tape
Shervani et al. Preparation of hydrogenated surfactant/SC CO2 micelles and their micropolarity determination
RU2571793C1 (en) Method for preparation of glass fusion charge
RU2119795C1 (en) Method for obtaining gamma-globulin