BE496426A - - Google Patents
Info
- Publication number
- BE496426A BE496426A BE496426DA BE496426A BE 496426 A BE496426 A BE 496426A BE 496426D A BE496426D A BE 496426DA BE 496426 A BE496426 A BE 496426A
- Authority
- BE
- Belgium
- Prior art keywords
- sep
- solvent
- liquid according
- tissue
- parts
- Prior art date
Links
- 239000002904 solvent Substances 0.000 claims description 32
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- FPZWZCWUIYYYBU-UHFFFAOYSA-N 2-(2-ethoxyethoxy)ethyl acetate Chemical compound CCOCCOCCOC(C)=O FPZWZCWUIYYYBU-UHFFFAOYSA-N 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 4
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 3
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 239000012188 paraffin wax Substances 0.000 description 13
- 238000011282 treatment Methods 0.000 description 9
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 8
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 8
- 238000007654 immersion Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000018044 dehydration Effects 0.000 description 5
- 238000006297 dehydration reaction Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 239000012024 dehydrating agents Substances 0.000 description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000008096 xylene Substances 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- BJINVQNEBGOMCR-UHFFFAOYSA-N 2-(2-methoxyethoxy)ethyl acetate Chemical compound COCCOCCOC(C)=O BJINVQNEBGOMCR-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000002828 effect on organs or tissue Effects 0.000 description 1
- WVNGEFSKQQHMLF-UHFFFAOYSA-N ethoxyethane;2-(2-hydroxyethoxy)ethyl acetate Chemical compound CCOCC.CC(=O)OCCOCCO WVNGEFSKQQHMLF-UHFFFAOYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Publications (1)
Publication Number | Publication Date |
---|---|
BE496426A true BE496426A (enrdf_load_stackoverflow) |
Family
ID=139662
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BE496426D BE496426A (enrdf_load_stackoverflow) |
Country Status (1)
Country | Link |
---|---|
BE (1) | BE496426A (enrdf_load_stackoverflow) |
-
0
- BE BE496426D patent/BE496426A/fr unknown
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4911915A (en) | Method of processing tissue specimens and dehydrant solvent for use therein | |
Ebbesson | The selective silver-impregnation of degenerating axons and their synaptic endings in nonmammalian species | |
JPH11513123A (ja) | 生物検体の処理方法 | |
US5318795A (en) | Staining methods for histology and cytology specimens | |
EP0442996A1 (fr) | Fleurs coupees de longue duree et procede de traitement pour l'obtention de telles fleurs | |
CN103512784B (zh) | 植物组织切片的制作方法、植物组织切片及其应用 | |
US20080268496A1 (en) | Composition for the preparation of histological, autopsical, cytological samples | |
CN115615783B (zh) | 石蜡组织切片的制备方法 | |
BE496426A (enrdf_load_stackoverflow) | ||
US2684925A (en) | Liquid for treating tissue in histologic processing | |
CN110132673A (zh) | 一种制备金针菇子实体组织切片的方法 | |
EP1000335B1 (fr) | Procede de fixation et d'enrobage de tissus destines aux preparations histologiques | |
JP2004099605A (ja) | 切花の保存方法 | |
CN115683697A (zh) | 一种植物韧皮部组织切片方法 | |
RU2593343C1 (ru) | Способ окраски гистологических срезов при диагностике трихинеллеза | |
Fish | Notes on Technique | |
BE496425A (enrdf_load_stackoverflow) | ||
Bell et al. | Microtechnique for winter buds | |
Hunter | Recent histological methods | |
EP0235832B1 (fr) | Procédé et installation perfectionnée pour le dégraissage de peaux brutes et peaux brutes obtenues | |
CH285195A (fr) | Procédé de préparation d'un tissu organique en vue de son examen microscopique. | |
JP2023164366A (ja) | 高品質黒目漆の製造方法 | |
Farris | A rapid method of sectioning dried coniferous needles for mycological studies | |
BE348898A (enrdf_load_stackoverflow) | ||
BE351465A (enrdf_load_stackoverflow) |