BE1030405B1 - COMPOSITION FOR DEGRADING ORGANIC MATERIAL, ITS MANUFACTURING METHOD AND USES THEREOF - Google Patents

Abstract

Composition for degrading organic matter comprising one or more aluminosilicate particles, one or more porous calcium carbonate particles, one or more trace elements, and one or more microorganisms, characterized in that at least one aluminosilicate of said one or more aluminosilicates is an aluminosilicate having a tetrahedral structure, in which the microorganisms adhere to said aluminosilicate having a tetrahedral structure and in which at least one of said microorganisms is mainly in sporulated form.

Description

COMPOSITION FOR DEGRADING ORGANIC MATERIAL, ITS PROCESS

MANUFACTURING AND USES THEREOF

Technical area

The present invention relates to a composition for carrying out biological purification of wastewater.

In particular, the present invention relates to a composition, for degrading organic material, comprising one or more aluminosilicate, one or more porous calcium carbonate, one or more trace elements, and one or more microorganisms.

The present invention also relates to a process for manufacturing the composition according to the present invention, to a process for degrading organic matter present in a sewer system, and to a use of the composition according to the present invention to liquefy and/or or reduce organic matter in sewers.

Technological background of the invention

Wastewater, which may include for example different types of pollutants, various wastes and organic matter, represents an increasingly important environmental problem, particularly due to the densification of the population in urban centers and/or the The intensification of industrialization increasingly increases the quantity of wastewater to be treated, which can lead to saturation and pollution of sanitation systems such as sewage systems.

Wastewater includes domestic wastewater from private homes, but also wastewater from small communities such as a hotel complex, campsite, housing estate, offices, residential buildings, small villages.

Wastewater also includes waste water from urban centers, industrial centers, agro-food waste water, and accidental pollution. Wastewater can also include rainwater, which includes polluted meteorite water and water that has flowed onto more or less permeable surfaces, thus increasing the pollution load. Generally, this wastewater is collected and evacuated by sanitation systems such as a system

IO sewers and treated by treatment plants.

Various pollutants, such as oils, animal and/or vegetable fats and, more generally, organic matter present in wastewater, can generate, when this wastewater passes through a sanitation system, agglomerates of materials. solids with sludge, which can significantly obstruct the circulation of wastewater in the sanitation system. In addition, the high load of organic matter present in wastewater is a significant source of pollution that can lead to eutrophication and/or algae proliferation phenomena.

Furthermore, depending on the different industries near the sanitation system, different types of chemical pollutants may also be present in the wastewater such as halogenated compounds, cresols, phenols, different surfactants, compounds based on lignin and/or cellulose, organochlorine compounds, cyanide compounds, etc.

Finally, wastewater and/or sludge contained in sanitation systems can be biologically polluted by a population of “endogenous” bacteria and/or microorganisms potentially harmful to the environment and even toxic for animals, or even for the human being such as for example strains of

Escherichia coli (E. coli), staphylococci, Salmonella, Listeria, etc... || It is therefore important to develop solutions to reduce wastewater pollution, to reduce and/or liquefy the quantity of sludge present in sanitation systems but also to prevent the appearance of pollution and/or the appearance sludge in sanitation systems.

State of the art

Physical solutions such as high pressure water jets and/or chemical solutions exist on the market to reduce agglomerates of solid matter and/or to liquefy the sludge present in sanitation systems. These physical and/or chemical solutions are, however, used to treat an obstruction problem and/or to clean up sanitation systems. These physical and/or chemical solutions are generally used as a means of treatment and not as a means of prevention to prevent a sanitation system blockage problem from occurring. These chemical solutions are also increasingly incompatible with sustainable and ecological management of wastewater and/or wastewater sanitation systems.

Water purification by biological treatment is also known from the prior art. Indeed, a composition of matter for the biological purification of polluted water is known from the document

EP0410877B1. This document describes a composition of matter, intended to be spread in the water to be purified and characterized in that it comprises at least the following two mineral materials, in granular form: a porous calcium carbonate, and an alumina silicate hydrated containing alkaline earth metals and presenting microchannels. In addition, these two materials contain in the adsorbed state specific bacteria for the biological degradation of organic materials with a carbon chain.

Favorably, the porous calcium carbonate having high porosity allows numerous ionic exchanges with the medium to be purified.

The second material, consisting of a hydrated alumina silicate, makes it possible to trap the ammonium ions contained in polluted water which are transformed into harmless molecules by the bacteria adsorbed in the material.

Although this document praises the merits of its technology, the inventors noted that, in practice, this composition has a limited action over time because the bacteria, necessary for the biological purification of organic matter present in wastewater and sewer sludge is not very stable and has a limited survival rate in a polluted environment comprising a high load of organic matter and/or chemical pollutants and/or biological pollutants such as sewer sludge therefore limiting the purification capacities biology of microorganisms.

Another attempt, according to document CN113149339, describes a composite material for the treatment of cyanobacterial blooms, characterized in that the composite material comprises clay minerals, flocculants and functional microorganisms, in which, the micro- functional organisms are loaded on clay minerals. Here too, although the composition has certain merits, the inventors have noticed that in practice for the treatment of wastewater and/or sewage sludge, the microorganisms present on the composite material are not very stable, with a survival rate and limited growth reducing the biological purification capabilities of the composite material.

Summary of the invention:

The present invention relates to a composition for degrading organic matter comprising: (i) particles comprising one or more aluminosilicate, (ii) particles of one or more porous calcium carbonate, (iii) one or more trace elements, and (iv) one or more microorganisms,

characterized in that at least one aluminosilicate of said one or more aluminosilicates is an aluminosilcate having a tetrahedral structure, and in that the microorganisms adhere to said aluminosilicate having a tetrahedral structure and in that at least one of said microorganisms 5 is mainly in sporulated form, preferably the microorganisms not being in sporulated form being freeze-dried and revivable and, preferably said one or more trace elements being present at a content of at least 5% by weight relative to said tetrahedral aluminosilicate (sum of the weight of the oligos -elements: weight of the tetrahedral aluminosilicate).

Detailed description of the invention:

The present invention aims to overcome the disadvantages of the state of the art by providing a composition for degrading an organic material according to the present invention characterized in that at least one aluminosilicate of said One or more aluminosilicate is an aluminosicate having a tetrahedral structure , in which the microorganisms adhere to the aluminosilicate having a teirahedral structure and in which at least one of said microorganisms being - mainly in sporulated form, preferably the microorganisms not being in sporulated form being freeze-dried and revivifiable and, preferably said one or several trace elements being present at a content of at least 5% by weight relative to said tetrahedral aluminosilicate (sum of the weights of the trace elements: weight of the tetrahedral aluminosilicate).

Indeed, a composition according to the present invention in which at least one aluminosilicate has a tetrahedral structure makes it possible to fix a significant quantity of microorganisms, and this in a lasting manner. The tetrahedral structure of Valuminosilicate also provides a micro-environment adapted to the microorganisms of the composition according to the invention ensuring their protection against a polluted external environment, such as for example the sludge present in sewer systems, rich in organic matter and/or already saturated with endogenous microorganisms. Furthermore, the fact that at least one of the microorganisms in the composition according to the invention is mainly in sporulated form provides great resistance to the latter. Consequently, a composition according to the present invention combining at least one aluminosilicate having a tetrahedral structure with at least one microorganism mainly in sporulated form makes it possible to improve the stability of the microorganisms, to increase their survival rate and also makes it possible to increase their proliferation capacity by providing them with an adequate microenvironment for their protection and growth. The stability and increased proliferation of the microorganisms of the composition according to the present invention allow these microorganisms to carry out effective and stable biological purification of wastewater and/or sludge present in sanitation systems such as sewer systems. The biological purification carried out using the composition according to the present invention provides not only a treatment solution but also a prevention solution to combat the obstruction of sanitation systems by sludge and agglomerates of solid materials. The composition according to the present invention also makes it possible to treat or prevent biological pollution by toxic microorganisms and/or chemical pollution by different types of chemical pollutants.

In the composition according to the present invention, preferably, at least 5%, more preferably, at least 10%, at least 20%, at least 30%, at least 50%, at least 60%, at least 80%, at least least 90%, at least 95% of the microorganisms being freeze-dried are revivable and form colonies.

The present invention also relates to a process for manufacturing the composition according to the present invention. The manufacturing process comprising the steps of: - a first mixture is produced comprising a culture of one or more microorganisms for a predetermined period of time at a predetermined temperature in the presence of, optionally a carbon base metabolizable by said one or more microorganisms and/ or a source of amino acids, one or more trace elements and one or more tetrahedral aluminosilicate, said first mixture having a water content of between 10 and 30% by weight, preferably between 20 and 25% by weight, - we carry out drying at a temperature between 10°C and 40°C of said culture so as to cause adhesion of said microorganism(s) in the porosity of said tetrahedral aluminosilicate, - a second mixture is produced by adding a second mixture to said first mixture composition comprising granular compounds having a water content less than 15% (water weight:weight of the second composition) being one or more porous calcium carbonate and/or one or more aluminosilicate, in which the water content of the first mixture is higher than the water content of the second mixture and in which the water content gradually decreases during the first and second mixing, for example to reach a final water content of the second mixture less than 10% (water weight:weight composition).

The present invention also relates to a method for degrading organic matter present in a sewer system. The process for degrading organic matter comprising: - a determination of the pollution load and/or the redox potential and/or the hydrogen sulfide level in several locations of a sewer system, including its ramifications,

- a determination of one or more points of administration of the composition according to the invention or of the composition obtained by implementing said manufacturing process according to the invention, - a distribution of a first predetermined quantity of said composition to said or several points of administration at a predetermined time, - Optionally, a second (subsequent) determination of the pollution load and/or the redox potential and/or the level of hydrogen sulphide in said sewer system, preferably followed by a series of booster dispensing of a second predetermined quantity of said composition to said one or more points of administration at predetermined time intervals after said predetermined time.

According to the present invention, the term "a determination of one or more points of administration of the composition" is preferably understood to mean a determination of one or more administration schemes of the composition comprising a determination of one or more locations. administration and/or a determination of the content of the composition to be administered and/or a determination of one or more quantities of the composition to be administered and/or a determination of one or more frequencies of administration of the composition to be administered administer.

According to the present invention, the term "a process for degrading organic matter present in a sewer system" is preferably understood to mean a process for liquefying and/or reducing and/or displacing a quantity of organic matter present in a sewage system. a sewerage system.

The present invention finally relates to a use of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention to liquefy organic matter from sewers.

Figure 1 is a schematic representation of the different stages of the process for manufacturing the composition according to the present invention.

Figure 2 is a schematic representation of the different steps of the process for degrading organic matter present in a sewer system according to the present invention.

Figure 3 shows a map of a sewer system

IO including multiple administration points and a checkpoint.

Advantageously, the composition according to the invention further comprises a nitrate salt, preferably a nitrate salt of an alkaline or an alkaline earth, advantageously comprising at least 5% by weight of nitrate expressed by weight NO3 - relative to the dry weight of the composition. In fact, the composition does not work in the dirtiest sewers, unless an adjuvant (RedOx) is added, for example a nitrate (potassium, calcium, Mg).

Preferably, at least one of the aluminosilicates is teirahedral, chosen from zeolites, preferably chabazite, phillipsite and/or stilbite, or feldspars. Particularly preferably, at least one of the tetrahedral silicates is a Zeolite (chabazite). In addition, the composition may comprise another tetrahedral aluminosilicate and/or a phylosilicate, preferably chosen from mica, smectite, kaolinite, vermiculites, glauconites, chlorites and their mixtures. Preferably, the one or more tetrahedral aluminosilicate is part of an aluminosilicate group comprising zeolites, preferably chabazite, feldspars or a mixture thereof, and/or further comprising one or more preferably selected phylosilicate among mica (vermiculites, glauconites), smectite, kaolinite, chlorites and their mixtures, preferably bentonite. Preferably, the composition comprises at least 20% of tetrahedral aluminosilicate (weight of tetrahedral aluminosilicates: total weight of aluminosilicates) and/or at least 5% of smectites (weight of smectites: total weight of aluminosilicates). More preferably, the composition comprises between 20% and 90%, even more preferably between 30% and 80%, of tetrahedral aluminosilicate (weight of tetrahedral aluminosilicates: total weight of aluminosilicates) and/or between 5% and 75%, of preferably between 10% and 50%, more preferably between 20% and 40% of smectites (weight in smectites: total weight in aluminosilicates). The inventors noticed that too abundant incorporation of phyllosilicates posed problems, which can partly be overcome by the addition of porous calcium carbonates. Advantageously, the composition comprises between 3% and 15%, preferably between 4% and 10%, more preferably between 5% and 8% of tetrahedral aluminosilicate (weight of the tetrahedral aluminosilicates: total dry weight of the composition) and/or between 1% and 25%, preferably between 3% and 15%, more preferably between 5% and 10% of smectites (smectite weight: total dry weight of the composition).

Preferably, one or more porous calcium carbonate is chosen from calcite (or a limestone rock) and a crushed shell, and preferably in which the composition comprises at least 5% smectites (weight in smectites: total weight in aluminosilicates) and/or at least 1% smectites (weight in smectites: total weight in aluminosilicate and limestone). Preferably, the composition comprises between 5% and 50%, more preferably between 10% and 40%, favorably between 15% and 35% of smectites (weight in smectites: total weight in aluminosilicates) and between 1% and 20% , preferably between 2% and 15%, favorably between 3% and 10% of smectites (weight in smectites: total weight in aluminosilicate and limestone).

Preferably, the composition according to the invention further comprises particles of pumice, for example rhyolite, dacite and/or andesite, and preferably in which the composition comprises at least 5% of pumice. (weight in pumice: weight in aluminosilcates and calcium carbonate). Preferably the composition comprises between 5% and 50%, favorably between 10% and 40%, of pumice (weight in pumice: weight in aluminosilicates and calcium carbonate). Preferably the composition comprises between 1% and 35%, favorably between 2% and 15%, or even between 5 and 10% of pumice (pumice weight: total dry weight of the composition).

The inventors noticed that tetrahedral aluminosilicates were particularly advantageous for housing the microorganisms according to the present invention (in the form of spores or freeze-dried) and ensuring their ability to be revived over time. Thus, preferably, the microorganisms of the present invention are found predominantly at the level of the tetrahedral aluminosilcate(s). On the other hand, the presence of other aluminosilcates is advantageous, in particular phylosilicates, for example so as to inhibit, or adsorb, heavy metals. Advantageously, the porous calcium carbonate(s) is chosen from calcite and/or crushed shellfish and/or sedimentary limestone. Indeed, this type of aluminosiicate and/or this type of calcium carbonate has a porous granular structure adequate to provide a microenvironment suitable for sporulation, survival and also the growth of the microorganisms of the composition according to the invention. Thus, even if these porosities of the complementary rocks of the tetrahedral aluminosilicate(s) do not include the microorganism of the invention in the form of spores or freeze-dried, these rocks offer structures which will be colonized as soon as the metabolism of the microorganisms will be reactivated (e.g. in the presence of organic matter).

The combination of tetrahedral aluminosilicate and the complementary rock(s) (phyllosiicate and/or calcium carbonate and/or pumice) is synergistic in that the combination optimizes the depollution spectrum by ensuring decontamination of great diversity of pollutants. In addition, the combination of the tetrahedral aluminosilicate and the complementary rock(s) also makes it possible to more easily buffer the pH, to fix the heavy metals present in the sewers, and to make the carbonate available to the microorganisms in the composition, which stimulates the metabolism of these, in particular of the microorganisms responsible for the nitrogen cycle.

Advantageously, the composition comprises one or more trace elements forming part of a group of trace elements comprising magnesium sulfate, iron sulfate, iron sulfate,

IO manganese, disodium hydrogen phosphate, ammonium sulfate, zinc sulfate, ferrige chloride, calcium sulfate, potassium sulfate, copper sulfate, sodium chloride, and mixtures thereof. Indeed, this type of trace elements provides nutrients to improve the micro-environment of the microorganisms in the composition, which promotes their growth. The nature and content of these trace elements can be adapted in the light of the present invention, for example by testing the growth properties of yophilized and/or spore-forming microorganisms as soon as they are put back into contact with their substrate (e.g. a organic matter, such as organic matter having similarities to that of a sewer, for example of the sewer system to be treated).

A typical trace element content is between 5 and 50%: weight of the trace elements above: weight of the teirahedral aluminosilicate (including microorganisms in the form of spores and/or freeze-dried) preferably between 10 and 40% . In the above ratios, preferably, the content of calcium carbonate and magnesium carbonate (incorporated at the same time as the limestone rock or crushed shellfish) is not taken into account and the calcium and magnesium considered for the calculation of the ratios above comes only from the other salts of these alkaline earth metals.

Preferably, the composition according to the invention comprises one or more microorganisms forming part of a group of microorganisms comprising Bacillus subtilis, Bacillus Licheniformis,

Pseudomonas putida, Pseudomonas fluorescens, Alcaligenes sp,

Acinetobacter sp, Arthrobacter sp, Nitrosomonas sp, Nitrobacter sp,

Lactobacillus helveticus, Lactococcus lactis, Pseudomonas sp, Botrytis cinerea, Sporotrichum dimorphosphorum, Trichoderma sp (Trichoderma reesei, Trchoderma harzianium, Trichoderma artroviride), Phanerochaete chrysosporium, Saccharomyces cerevisiae, Rhodococcus erythropolis,

Paracoccus sp and their mixtures. Indeed, these different microorganisms have good wastewater purification properties by degrading organic matter and/or reducing the pollution of certain chemical compounds such as plastics or hydrocarbons. Advantageously, the microorganism(s) of the composition is chosen according to the nature of the pollution to be treated in the wastewater and/or in the sewer sludge. Indeed, the inventors have discovered that the composition according to the invention preferentially comprising Pseudomonas Putida and

Acinetobacter sp is suitable for the degradation of halogenated compounds in wastewater and/or sewage sludge. They also discovered that a composition according to the invention preferably comprising (1)

Pseudomonas putida is adapted to the degradation of cresols and phenols, (ii) Lactobacillus helveticus and Lactococcus lactis is adapted to the degradation of animal and vegetable fats, (ii) Bacillus licheniformis is adapted to the degradation of surfactants, (iv) Botrytis cinerea,

Sporotrichum dimorphosphorum, Trichoderma reesei and Phanerochaete chrysosporium are adapted to the degradation of lignin and cellulose, (v) Alcaligenes sp and Pseudomonas sp are adapted to the degradation of organo-chlorine compounds, (vi) Alcaligenes sp,

Arthrobacter spl, and Trichoderma Harzianium! are suitable for the degradation of organo-polychlorinated compounds, (vi) Pseudomonas putida and/or Pseudomonas fluorescens and/or Trichoderma sp and/or

Rhodococcus are adapted to the degradation of vegetable fats and hydrocarbons.

In addition to the capabilities described above, the inventors have noticed that a combination of microorganisms in sporulated form

(e.g. Gram+ bacteria, yeasts, fungi) and others, such as Gram- bacteria (e.g. Pseudomonas sp.), was advantageous: the latter act more quickly when the composition of the invention is applied to the area to be decontaminated (sewers, fats to liquefy). Then, when the microorganisms in sporulated form have germinated, they develop their activity, often much higher and more lasting: this allows early activity and also lasting, as well as robust.

Thus, microorganisms having the property of forming spores are advantageously formulated in synergy with microorganisms which do not form spores (such as formulations involving one of the Bacillus described above and/or a Lactobacillus described above and one or several of the Pseudomonas described above).

Inventors have achieved such formulations in different ways. In particular, the inventors take into account the different germination and sporulation times, depending on the microorganisms, so that the slower germination microorganisms have sufficient time in the presence of nutrients (carbon and/or source of nitrogen) and sufficient moisture content, and the same for sporulation: the drying time is appropriate.

When all the microorganisms in the composition are in liquid form (germinated living and/or non-sporulated), they are advantageously incorporated by vaporization onto the support comprising the tetrahedral aluminosilicate before slow drying, as described (essentially below, by example step E2 and/or step E3).

An advantageous alternative is to make a mixture of microorganisms in the form of spores on a dry carbon source (or to obtain such a mixture), such as a maltodextrin, then to add this to the first mixture (wet; 2.4) . The bacteria will colonize the support (the macropores) during mixing during the following steps, such as the second mixing (2.8).

Combinations are possible: the microorganisms in the form of spores mixed with a dry carbon source are advantageously added to the first mixture (wet; 2.4), and then the microorganisms in liquid form (live, germinated and/or non-sporulated) are advantageously added. applied by spraying in steps E2 and/or E3.

Alternatively, the microorganisms in the form of spores mixed with a dry carbon source can also be incorporated into the second mixture 2.8 and/or the composition 2.9, when this second mixture 2.8 and/or this composition 2.9 has a particle size which allows the existence of macropores capable of housing the maltodextrin grain with the sporulated microorganisms, and then the microorganisms in liquid form (live, germinated and/or non-sporulated) are advantageously applied by vaporization in steps E2 and/or E3.

Favorably, the particles of one or more aluminosiicate and/or the particles of one or more porous calcium carbonate comprise a population of particles having a determined average particle size of between 20 um and 6000 um, preferably between 25 um and 4000 um, more preferably between 30 um and 3000 um, even more preferably between 40 um and 1500 um, between 50 um and 1000 um, favorably between 75 um and 800 um. Indeed, the inventors have discovered that small particles make it easier to insert themselves into sewer sludge and also allow them to be carried into the wastewater flow, which promotes exchanges between wastewater, sludge and particles increasing the biological purification process. Smaller particle sizes also have a greater surface/volume ratio, which also promotes exchanges between the particles and the polluted external environment. Favorably, the population of particles having a determined average particle size represents more than 50% (by weight: weight of the particles of the desired particle size: total weight of the particles), preferably more than 70%, more preferably more than 80%, favorably more than 90% of all of said particles of one or more aluminosilicate and/or of all of said particles of one or more porous calcium carbonate. Indeed, the higher the proportion of small-sized particles, the greater the specific surface area of exchange between the particles and the external medium, which favors the biological purification process. On the other hand, finer particles will tend to be carried away by the flow, while larger particles will remain longer at or near the application location. In addition, the particle size as described above allows the particles of the invention to accumulate in congested places in the sewers, where the flow is slowed, which ensures significant local activity where the situation is problematic; these particles will then be carried further when circulation is restored.

Figure 1 generally illustrates the different stages of the process for manufacturing the composition according to the present invention.

In a first step El, a culture of one or more microorganisms 2.1 is mixed with one or more trace elements 2.2 and one or more tetrahedral aluminosilicate 2.3 for a predetermined period of time between 10 min and 720 min, preferably between 15 min and 480 min, favorably between 20 min and 240 min and at a predetermined temperature advantageously between 5 °C and 40 °C, preferably between 18 °C and 37 °C, favorably between 20 °C and 30 °C for form a first mixture 2.4. Preferably, in the process for manufacturing the composition according to the present invention, the microorganism(s) 2.1 comprises one or more psychrophilic bacteria and said predetermined temperature is between 2°C and 25°C, preferably between 5°C and 20°C. °C, favorably between 10°C and 17°C. A carbon base 2.5 metabolizable by said one or more microorganisms and a source of amino acids 2.11 are added to the first mixture 2.4. For example, the carbon base includes edible potato starch. For example, the source of amino acids includes fish meal. In a second step E2, drying is carried out at a temperature between 10°C and 40°C of the first mixture 2.4 by adding to this first mixture 2.4 dry granular compounds of one or more porous calcium carbonate 2.6 (limestone rock and/or crushed shellfish and/or pumice), preferably having a humidity level of less than 15%, preferably less than 5%, favorably less than 2% (by weight of water relative to the weight of the compounds dry granular particles of one or more porous calcium carbonate 2.6), and/or one or more aluminosilicate 2.7 to form a second mixture 2.8. In a third step E3, this second mixture 2.8 is dried at a temperature below 40°C until a determined humidity level is obtained to form composition 2.9 according to the present invention. Favorably, the humidity level of composition 2.9 is less than 10%, preferably less than 7% (by weight of water relative to the weight of the composition). Advantageously, the humidity level of the composition after drying is between 2 and 10%, preferably between 3 and 8% (weight in water: weight of the composition).

It should be noted that slow drying as carried out during steps E2 and E3 of the present invention allows the sporulating bacteria to sporulate and adhere effectively to the tetrahedral aluminosilicate. In addition, slow drying as carried out in the manufacturing process according to the present invention allows a high survival rate of spore-forming bacteria, which will ensure subsequent germination of more than 60% (number of colonies per number of spores seeded), preferably by more than 70%, more preferably by more than 80%, favorably by more than 90% of the spore-forming bacteria. Slow drying also ensures acceptable viability for bacteria which do not sporulate, for example a viability of at least 10%, preferably at least 20% (number of colonies relative to the number of bacteria seeded.

Optionally, an addition of a nitrate salt 2.10 is carried out concomitantly with the drying step or after the drying step. The addition of a nitrate salt in the second mixture 2.8 or in the composition 2.9 according to the present invention is advantageous when the medium to be purified is poor in oxygen and/or has a low redox potential, typically less than - 50 mV, or even -100 mV. Preferably, the nitrate salt is a sodium nitrate or a calcium nitrate. The quantity of tetrahedral aluminosilicate 2.3 in the first mixture is typically between

50 and 80% by weight (weight of the aluminosilicate: weight of the first mixture 2.4). The quantity of the mixture of trace elements 2.2 is typically between 20 and 40% by weight (dry weight of the mixture of trace elements 2.2: weight of the first mixture 2.4). Advantageously, the higher the quantity of the mixture of trace elements 2.2 relative to the weight of the first mixture, the lower the water content of the first mixture, which will promote the drying of the first mixture during the process of manufacturing the composition. Typically from 0.5 to 1 unit by weight of the first mixture 24 is taken up and mixed with 1 unit

IO of aluminosilicates, such as phyllosilicates, with from 0 to 6 units of porous calcium carbonate 2.6. The quantity of nitrate salt 2.10 to add can be zero, but can go up to 10 by weight (weight of nitrate salt 2.10: sum of the dry weights of all the other components of the second mixture 2.8). Typically from 1 to 4 units by weight of nitrate salt are added per unit of the mixture above (0.5 to 1 unit by weight of the first mixture 2.4 is taken up and mixed with 1 unit of aluminosilicates, such as phyllosilicates, with 0 to 6 units of porous calcium carbonate 2.6).

Thus, the content of aluminosilicate(s) in the composition of the invention 2.9 varies from 5% to 50% by weight (weight of the aluminosilicates: total weight of the mixture), preferably from 10% to 20%. These aluminosilicates can be mostly of the tetrahedral type, preferably between 20% and 100% of the aluminosilicates are tetrahedral, for example between 25% and 50%, or between 30% and 40% (weight of tetrahedral aluminosilicates: total weight of aluminosilicates ). Thus, the content of tetrahedral aluminosilicates in the composition of the invention 2.9 preferably varies between 3% and 15%, preferably between 4% and 10%, more preferably between 5% and 8% (weight of the tetrahedral aluminosilicates: total dry weight of composition 2.9).

Figure 2 generally illustrates the different steps of the process for degrading organic material according to the present invention. Firstly, the pollution load and/or redox potential and/or hydrogen sulphide level in the sewer system, including its ramifications 3.1, is determined. Preferably, according to the present invention, the pollution load is measured by tests via measuring probes. The redox potential is measured for example using probes measuring the redox potential, ORP probes.

Advantageously, the pH is also measured using a measuring probe. Preferably, the oxygen level is measured using measuring probes such as for example a luminescence probe to measure the dissolved oxygen level. To determine the load of sewer pollution, several ratios will be determined using measuring probes at different locations in the sewer system. A first ratio (ratio) is for example the biological oxygen demand (BOD) divided by the chemical oxygen demand (COD) which measures the bioavailability of pollution. Firstly, the composition according to the invention (e.g. comprising nitrates) makes it possible to improve (locally) the redox potential of the polluted environment, making this environment more favorable for bioconversion using the microorganisms in the composition, and sometimes even by microorganisms naturally present in the sewers.

Advantageously, using the composition according to the invention and for an identical quantity of pollution, that is to say a constant COD, the ratio

BOD/COD increases initially following administration of the composition into the sewer system. After several months of treatment, this BOD/COD ratio decreases sharply and can reach 0.25 after | year of administration of the composition. Indeed, the microorganisms in the composition will be very active and will degrade the available organic matter, thereby ensuring a reduction in the BOD/COD ratio. A second ratio (ratio) is for example the quantity of ammoniacal nitrogen (NH3) divided by the quantity of total Kjeldahl nitrogen (TKN) present in the polluted medium. Total Kjeldahl nitrogen (TKN) is the sum of organic nitrogen, ammonia (NH3) and ammonium (NH4+).

Advantageously, the microorganisms in the composition transform the organic nitrogen of the polluted environment (sewage system) into ammonia (dissolved in water in the form of ammonium ions). These ammonium ions are then more easily oxidized to nitrites and nitrates by nitrifying bacteria present in the sewer system. Accordingly, this second NH3/TKN ratio increases after administration of the composition into the sewage system because the microorganisms efficiently transform the organic nitrogen present in the sewage system.

Advantageously, in the process for degrading an organic material according to the present invention, the one or more tetrahedral aluminosilicate of the first mixture is part of a tetrahedral aluminosilicate group comprising zeolites and/or feldspars and in which said granular compounds of said second composition is part of a group of granular compounds comprising tetrahedral aluminosilicates and/or phyllosilicates and/or porous calcium carbonate and/or pumice, wherein said one or more tetrahedral aluminosilicates being preferentially present at a content of at least 10% by weight relative to the weight of said composition.

Advantageously, the content of nitrate salt 2.10 in the second mixture 2.8 is adapted as a function of the measured redox potential, if the redox potential is low, for example close to -100 MV, up to 40% by weight of nitrate salt 2.10 will be added to form the second mixture 2.8. Lower contents are applied if the redox potential is -50 mV.

One or more points of administration 3.1 of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention are also determined. Furthermore, the possible presence of compounds harmful to microorganisms (e.g. microbiocides) is sought and, preferably, the composition according to the invention is not applied at the level of the branches comprising significant contents of deleterious compounds: the composition according to This invention will preferably be administered to other branches of the sewer system in which the deleterious compounds are less present (preferably absent), or are diluted due to the confluence of the branches.

Preferably, the determination of one or more administration points 3.1 includes a mapping of the sewer system and/or the entry points of the sewer system and/or the presence of one or more industrial sites near the sewer system and/or the presence of one or more residential areas near the sewer system and/or the presence of compounds deleterious to the metabolism of the added microorganisms (microorganisms present in the composition).

A distribution 3.2 of a first predetermined quantity of the composition is then carried out at one or more determined administration points 3.2 of the sewer system, including its ramifications, at a predetermined time. Preferably, the first predetermined quantity is between 0.1 ppm and 100 ppm, preferably between] and 10 ppm, more preferably between 2 and 5 ppm (weight of the composition: weight of the material having circulated in the branch of the drain between 2 dosages).

Preferably, concomitantly with the administration of the first predetermined quantity of the composition, an oxidant, a chemical oxidant, is added at one or more points of administration.

The addition of an oxidant makes it possible to increase the redox potential of wastewater and sewer sludge, which will promote the biological purification process by the microorganisms of the composition according to the invention.

Adding an oxidant is useful when the redox potential is too low, for example when the redox potential is less than -50mV.

Advantageously, when the redox potential of the polluted environment (sewer system) is low, typically equal to or less than -50 mV, the addition of oxidant makes it possible to increase the redox potential to values greater than -50mV.

Advantageously, an increase in the redox potential of the polluted environment creates a favorable environment allowing the microorganisms of the composition according to the invention to oxidize the organic matter present in the polluted environment. The oxidation of the organic matter by the microorganisms of the composition has the advantageous consequence of increasing even more the redox potential of the polluted medium so that a synergy is present between the addition of oxidant and the presence of the microorganisms of the composition. composition according to the invention. Preferably, the oxidant added when the redox potential is low, equal to or less than -

SOMV, is part of an oxidant group including nitrate salts, calcium nitrate, potassium nitrate, sodium nitrate, permanganate salts. Hypochlorite and/or chlorate are not preferred as an oxidant. Advantageously, if hypochlorite and/or chlorate are added to the polluted environment, one or more additions of microorganisms will then be made so as to repopulate the environment with microorganisms.

Preferably, the distribution 3.2 of a first predetermined quantity of the composition in the sewer system, including its ramifications, is carried out at least 2 days before or at least 2 days after significant precipitation defined by a precipitation rate of 8 mm per hour or more. Advantageously, the distribution 3.2 of a first predetermined quantity of the composition in the sewer system is not carried out during the period of leaching of the sewer system, in particular if the residence time of a quantity of water in the sewerage system decreases by 50% or more compared to the residence time of the same amount of water in the sewerage system in dry weather.

Optionally, after a predetermined period of time between 40 days and 60 days, preferably between 45 days and 50 days, a second determination 3.3 of the pollution load and/or the redox potential and/or the rate is carried out. of hydrogen sulfide in the sewer system. Preferably, the second determination 3.3 is followed by a series of booster distribution 3.4 of a second predetermined quantity of the composition at one or more points of administration at predetermined time intervals after said predetermined moment. Preferably, the first predetermined quantity is greater than the second predetermined quantity.

Preferably, the one or more administration points of the booster distribution series 3.4 are identical to the one or more administration points 3.1 of the composition. Advantageously, a booster administration of the composition is carried out weekly at one or more administration points of the booster distribution series 3.4.

The present invention also relates to a use of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention for liquefying and/or reducing organic matter in a sewer system.

The present invention also relates to a use of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention to reduce the quantity of one or more chemical pollutants present in a system of sewers.

The present invention also relates to a use of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention to reduce the quantity of one or more toxic endogenous microorganisms (for example strains of E. coli, Bacillus cereus, Salmonella sp. or even Listeria sp. normally abundant in sewers) present in a sewer system and/or in an aquatic area.

By the term “toxic endogenous microorganisms” is meant, according to the present invention, microorganisms developing in a sewer system and/or in an aquatic area such as, for example, a pond, a river, a port area, a canal, a river, an aquarium and being toxic to animals and/or humans.

The present invention also relates to a use of the composition according to the invention or of the composition obtained by implementing the manufacturing process according to the invention to reduce the organic matter present in an aquatic area such as for example, a pond, A river, a port area, a canal, a river, an aquarium.

Other characteristics and advantages of the present invention will be derived from the following non-limiting description, and with reference to the drawings and examples.

Examples.-

Example 1. Composition for degrading organic material according to the present invention and its manufacturing process

The table | describes the proportion of aluminosilicate as well as the nature and proportion of the different trace elements present in the first mixture 2.4.

Table 1: Proportion of aluminosilicate and trace elements present in the first mixture 2.4.

Compounds Froportion (%o by mass}

Aluminosilicate 300-890 tehrahedral

Los of the first mixture 24 the tetrahedral aluminosiicaies are mixed with the trace elements in the proportions indicated in the table | considering a total quantity of | kg. A quantity of 10419 microorganisms comprising 20-60% Bacillus sutotilis, 2-25% Pseudomonas Putida, 2-25% Bacillus licheniformis and a quantity of | g of carbon base metabolizable by microorganisms (source of AA) are added to this first mixture. It is advantageous that this first mixture comprises or less one psychrophilic bacteria.

The first mixture is carried out for 24 hours at a

IO temperature of 20°C with constant stirring.

During the second mixture, 150 kg of porous calcium carbonate (limestone), 100 kg of sodium nitrate and 25 kg of aluminosilicate are added to 12.5 kg of the first mixture 2.4 to give the second mixture 28. This second mixture 28 is then dried at a temperature of 20°C for 24 hours to obtain a composition having a humidity level of less than 7% {weight in water: weight of second mixture).

Example 2.- Particle size distribution of the composition according to the present invention

The composition according to the invention obtained in the Example has an average particle size distribution of between 50 and 160 μm. In order to obtain this fine grain size, the particles of aluminosilicate and limestone are purchased having the appropriate properties. This fine particle size allows the particles of the composition according to the invention to be both sufficiently mobile but also to be able to be deposited in sewer sludge. This granulation allows the particles of the composition good penetration into the sludge of the sewers, also allows them to become embedded in the different crevices of the pipes of the sewer system and also allows a proportion of the particles to remain in the water flow. , which ensures a good distribution of the composition following

Invention in pipes.

Example 3.- Determination of one or more points of administration of the composition according to the invention

Using a map of the pipe network of a private sewer system (private industrial site), including its ramifications as well as the location of an industrial site and an urban area (see figure 3}, the inventors have determined 6 points of administration 3.1 of the composition described in Example 1 (see Figure 3}. These points 10. of administration 3.1 allow optimal distribution of the composition in the sewer system st therefore ensures adequate degrodation {liguefaction and/or displacement and/or reduction) of the sludge and organic matter contained in the sewer system. The arrows in Figure 3 show the direction of progression of the water in the sewer system, A control point 4.1 located downstream of the sewer system makes it possible to monitor, using probes, the level of pollution in the sewer system.

Example 4.- Liouefaction of sludge present in a sewer system using the composition according to the present invention.

After 200 days from the first administration of the composition according to the invention (see example 3), the quantity of sludge in the sewer system was reduced (by liquefaction and/or by migration, displacement) by 35% (quantity of sludge after 200 days of lg first administration of lg composition: quantity of sludge on the day of the first administration) ensuring better evacuation of waste water present in the sewer system.

Example 58- Reduction of the quantity of potentially toxic endogenous bacteria present in a sewer system by using the composition according to the present invention in a private place, industrial site! private, not accessible to the public.

On June 25, the inventors determined the load of bacterial pollution at 5 different points of administration: sewer 1, sewer 2, sewer 3, sewer 4 and sewer 5. In particular, they measured the number of colonies formed per unit of surface (25 om) of E coli, Staphylococci,

Bacillus cereus, Saimonelia and Listeria (see Table 2). The inventors then administered, on June 25, a first predetermined quantity of 0.020 kg of the composition according to example 1 at each of the 5 administration points, and then 0.020 kg of the composition following the example at each of the 5 points of administration 1 time per week More or less 1 month later, on July 23, the inventors again measured the load of bacterial pollution at the 5 points of administration and observed a significant reduction, greater than 50-fold, in the quantity of E. col,

Staphylocogues and Bacillus cereus [see Table 2}.

Table 2: Samplings (SWAB) taken from the most structurally dirty sewers

Date: sample E. coli Staphylococci Bacillus Salmonella Listeria {(CFU/25 | (CFU/25 cm?) cereus (CFU/25 (CFU/25 cm?) (CFU/25 | cm?) cm?) cm?)

Example e.- Composition according to the invention for degrading organic matter present in a sewer system presenting high grease pollution

The sewer system included or Initial time, before! administration of the composition according to the invention, a layer of fat more than 10 cm thick. The inventors applied, at time T = 0, a first administration of 300 kg of the composition following the example | upstream of the sewer system, in which the composition further comprises at least 20% of lactic acid bacteria relative to the total quantity of microorganisms in the composition according to example 1. A repeated administration, once a week, of 150 kg of the same composition was carried out upstream of the sewer system for 8 months.

After 8 months of treatment, the layer of grease in the sewer system was reduced by almost 80% to give a layer of grease 2 cm thick (compared to 10 cm thick before treatment).

Example 7.- Composition according to the invention for degrading organic matter present in a sewer system presenting high hydrocarbon pollution

The sewer system was characterized or initial time, before administration of the composition according to the invention, significant hydrocarbon pollution. The inventors applied, at time T = 0, a first administration of 300 g por m? of the composition following the example | in which the composition further comprises at least 10% of pseudomonas bacteria relative to the total quantity of

Mmicroorganisms of the composition following Vexernple 1. A repeated administration, once a week, of 20kg of the same composition was carried out for 2 months. After 2 months of treatment, the quantity of hydrocarbons present in the sewer system had decreased by more than 90%.

Counter-example 1,- Liquid solution of bocteria to degrade organic matter present gifts A sewer system

Contrary to the addition of a composition according to the invention, the addition of a liquid solution containing micro-organisms upstream of a sewer system does not make it possible to reduce, by liquefaction and/or by displacement, the quantity grease and/or the amount of hydrocarbons in the sewer system. Indeed, a liquid composition containing bacteria does not work if specific enzymes for the biodegradation of organic matter are not added to the liquid composition. The inventors deduce that the liquid composition administered in the sewer system has too short a residence time in this sewer system preventing the microorganisms of the composition from being able to attach correctly to the sewer sludge and therefore preventing effective biodegradation.

It is of course understood that the present invention is in no way limited to the embodiments described above and that many modifications can be made without departing from the scope of the appended claims.

Claims (21)

1. Composition for degrading organic matter comprising: (i) particles comprising one or more aluminosilicate, (ii) particles of one or more porous calcium carbonate, (iii) one or more trace elements, and (iv) a or more microorganisms, characterized in that at least one aluminosilicate of said one or more aluminosilicate is An aluminosilicate having a tetrahedral structure, and in that the microorganisms adhere to said aluminosilicate having a tetrahedral structure and in that at least one of said microorganisms being mainly under sporulated form, preferably the microorganisms not being in sporulated form being yophilized and revivable and, preferably said one or more trace elements being present at a content of at least 5% by weight relative to said tetrahedral aluminosilicate (sum of weight of trace elements: weight of tetrahedral aluminosilicate). 2. The composition according to claim 1, further comprising a nitrate salt, preferably a nitrate salt of an alkaline or an alkaline earth, advantageously comprising at least 5% by weight of nitrate expressed by weight NO: relative to the dry weight of the composition. 3. The composition according to claim 1 or claim 2, wherein said one or more tetrahedral aluminosilicate is part of an aluminosilicate group comprising zeolites, preferably chabazite, feldspars or a mixture thereof, and /or further comprising one or more phyllosiicate preferably chosen from mica (vermiculites, glauconites), smectite, kaolinite, chlorites and mixtures thereof, preferably bentonite, preferably, the composition comprises at least 20% tetrahedral aluminosilicate (weight of tetrahedral aluminosilicates: total weight of aluminosilicates) and/or at least 5% of smectites (weight of smectites: total weight of aluminosilicates). 4. The composition according to any one of claims 1 to 3, in which said one or more porous calcium carbonate is chosen from calcite and a crushed shell, and preferably in which the composition comprises at least 5% smectites (weight of smectites: total weight of aluminosilicates) and/or less than 1% of smectites (weight of smectites: total weight of aluminosilicate and calcium carbonate). 5. The composition according to any one of claims 1 to 4, further comprising particles of pumice preferably comprising rhyolite, dacite and/or andesite, and preferably in which the composition comprises at least 5% pumice (weight of pumice: weight of aluminosilicates and calcium carbonate). 6. The composition according to any one of claims 1 to 5, in which said one or more trace elements is part of a group of trace elements chosen from the group consisting of magnesium sulfate, iron sulfate, manganese sulfate, disodium hydrogen phosphate, ammonium sulfate, zinc sulfate, ferric chloride, calcium sulfate, potassium sulfate, copper sulfate, sodium chloride, and mixtures thereof . 7. The composition according to any one of claims 1 to 6, in which said one or more microorganisms is part of a group of microorganisms comprising Bacillus subtilis, Bacillus licheniformis, Pseudomonas putida, Pseudomonas fluorescens, Alcaligenes sp, Acinetobacter sp, Arthrobacter sp, Nitfrosomonas sp, Nitrobacter sp, Lactobacillus helveticus, Lactococcus lactis, Pseudomonas sp, Botrytis cinerea, Sporotrichum dimorphosphorum, Trichoderma sp, Phanerochaete chrysosporium, Saccharomyces cerevisiae, Rhodococcus erythropolis, Paracoccus sp and mixtures thereof. 8. The composition according to any one of the preceding claims, in which said particles of one or more aluminosilcate and/or said particles of one or more porous calcium carbonate comprise a population of particles having a determined average particle size of between 20 µm and 6000 um, preferably between 25 um and 4000 um, more preferably between 30 um and 3000 um, even more preferably between 40 um and 1500 um, between 50 um and 1000 um, favorably between 75 um and 800 um. 9. The composition according to claim 8, in which said population of particles having a determined average particle size represents more than 50% (weight of the particles of the defined particle size: total weight of the particles), preferably more than 70%, more preferably more than 80%, favorably more than 90% of all of said particles of one or more aluminosilicate and/or all of said particles of one or more porous calcium carbonate. 10. Process for manufacturing a composition according to any one of claims 1 to 9, comprising the steps of: - a first mixture is produced comprising a culture of one or more microorganisms for a predetermined period of time at a predetermined temperature in presence of, optionally a carbon base metabolizable by said one or more microorganisms and/or a source of amino acids, one or more trace elements and one or more tetrahedral aluminosilicate, said first mixture having a water content of between 10 and 30% by weight, preferably between 20 and 25% by weight, - the composition is dried at a temperature of between 10°C and 40°C of said culture so as to cause adhesion of said microorganism(s) in the porosity of said tetrahedral aluminosilicate, - a second mixture is produced by adding to said first mixture a second composition comprising granular compounds having a water content less than 15% (water weight: weight of the second composition) comprising one or more porous calcium carbonate and/or one or more aluminosilicate, in which the water content of the first mixture is higher than the water content of the second mixture and in which the water content gradually decreases during the first and second mixing, preferably to reach a final water content of the second mixture less than 10% (water weight: composition weight). 11. The method according to claim 10, wherein said one or more tetrahedral aluminosilicate of the first mixture is part of a tetrahedral aluminosilicate group comprising zeolites and/or feldspars and wherein said granular compounds of said second composition is part of a group of granular compounds comprising tetrahedral aluminosilicates and/or phyllosicates and/or porous calcium carbonate and/or pumice, in which said one or more tetrahedral aluminosilicates being preferentially present at a content of at least 10 % by weight relative to the weight of said composition. 15 12. The method according to claim 10 or claim 11, wherein said predetermined culture time period is between min and 720 min, preferably between 15 min and 480 min, favorably between 20 min and 240 min. 13. The method according to any one of claims 10 to 12, in which said predetermined temperature is between 5°C and 40°C, preferably between 18°C and 37°C, favorably between 20°C and 30°C. °C. 14. The method according to any one of claims 10 to 13, in which said one or more microorganisms comprises one or more psychrophilic bacteria and in which said predetermined temperature is between 2 °C and 25 °C, preferably between 5 °C and 20°C, favorably between 10°C and 17°C. 15. The process according to any one of claims 10 to 14, in which the humidity level of said composition after drying is between 2 and 10%, preferably between 3 and 8% (weight in water: weight of the composition ). 16. The method according to any one of claims 10 to 15, comprising the incorporation of a nitrate salt and/or pumice stone into the second mixture. 17. Method for degrading organic matter present in a sewer system comprising the steps of: - determining the pollution load and/or the redox potential and/or the hydrogen sulfide level at several locations in said system sewer, including its ramifications, - A determination of one or more points of administration of the composition according to any one of claims 1 to 9 or of the composition obtained by implementing said manufacturing process according to one any of claims 10 to 16, - A distribution of a first predetermined quantity of said composition to said one or more points of administration at a predetermined time, - Optionally, a second determination of the pollution load and/or the redox potential and/or the level of hydrogen sulfide in said sewage system, preferably followed by a series of booster dispensing of a second predetermined quantity of said composition to said one or more points of administration at time intervals predetermined after said predetermined moment. 18. The method of claim 17, wherein said determination of one or more points of administration comprises mapping of the sewer system and/or entry points to the sewer system and/or the presence of a or more industrial site near the sewer system and/or the presence of one or more residential areas near the sewer system and/or the presence of compounds deleterious to the metabolism of the microorganisms present in the composition according to any one of claims 1 to 9 or of the composition obtained by implementing said manufacturing process according to any one of claims 10 to 16. 19. The method according to claim 17 or claim 18, in which the first predetermined quantity is between 0.1 ppm and 100 ppm (weight of the composition: weight of the material having circulated in the branch of the sewer between 2 dosages), preferably between 1 and 10 ppm, more preferably between 2 and 5 ppm. 20. The method according to any one of claims 17 to 19, wherein the first predetermined quantity is greater than the second predetermined quantity. 21. Use of the composition according to any one of claims 1 to 9 or of the composition obtained by implementing said manufacturing process according to any one of claims 10 to 16 to liquefy organic matter from sewers.