AU9253898A - Use of proteases in passaging of adherent animal or human cell cultures - Google Patents

Use of proteases in passaging of adherent animal or human cell cultures

Info

Publication number
AU9253898A
AU9253898A AU92538/98A AU9253898A AU9253898A AU 9253898 A AU9253898 A AU 9253898A AU 92538/98 A AU92538/98 A AU 92538/98A AU 9253898 A AU9253898 A AU 9253898A AU 9253898 A AU9253898 A AU 9253898A
Authority
AU
Australia
Prior art keywords
passaging
proteases
cell cultures
human cell
adherent animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU92538/98A
Inventor
Leif Kongerslev
Lars Skriver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of AU9253898A publication Critical patent/AU9253898A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU92538/98A 1997-09-26 1998-09-22 Use of proteases in passaging of adherent animal or human cell cultures Abandoned AU9253898A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK110797 1997-09-26
DK1107/97 1997-09-26
DK1389/97 1997-12-02
DK138997 1997-12-02
PCT/DK1998/000408 WO1999016865A1 (en) 1997-09-26 1998-09-22 Use of proteases in passaging of adherent animal or human cell cultures

Publications (1)

Publication Number Publication Date
AU9253898A true AU9253898A (en) 1999-04-23

Family

ID=26065241

Family Applications (1)

Application Number Title Priority Date Filing Date
AU92538/98A Abandoned AU9253898A (en) 1997-09-26 1998-09-22 Use of proteases in passaging of adherent animal or human cell cultures

Country Status (2)

Country Link
AU (1) AU9253898A (en)
WO (1) WO1999016865A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD159181A1 (en) * 1981-05-28 1983-02-23 Akad Wissenschaften Ddr METHOD FOR CULTIVATING CELLS
RU2039816C1 (en) * 1992-08-05 1995-07-20 Филиал Института биоорганической химии им.М.М.Шемякина РАН Preparation for detaching contact-dependent cells from substrate
US5422261A (en) * 1993-04-16 1995-06-06 Baxter International Inc. Composition containing collagenase and chymopapain for hydrolyzing connective tissue to isolate cells
DE4408940A1 (en) * 1994-03-16 1995-10-05 Boehringer Mannheim Gmbh Recombinant type II collagenase from Clostridium histolyticum and its use for the isolation of cells and cell assemblies
DE4445891A1 (en) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Recombinant proteinase from Clostridium histolyticum and its use for the isolation of cells and cell assemblies

Also Published As

Publication number Publication date
WO1999016865A1 (en) 1999-04-08

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase