AU7906800A - Multiplex pcr for detecting ehec infections - Google Patents

Multiplex pcr for detecting ehec infections

Info

Publication number
AU7906800A
AU7906800A AU79068/00A AU7906800A AU7906800A AU 7906800 A AU7906800 A AU 7906800A AU 79068/00 A AU79068/00 A AU 79068/00A AU 7906800 A AU7906800 A AU 7906800A AU 7906800 A AU7906800 A AU 7906800A
Authority
AU
Australia
Prior art keywords
sequences
stxa2
multiplex pcr
ehec infections
pathogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU79068/00A
Inventor
Tobias Bellin
Florian Gunzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytonet GmbH and Co KG
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of AU7906800A publication Critical patent/AU7906800A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrotherapy Devices (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Time-Division Multiplex Systems (AREA)
  • Burglar Alarm Systems (AREA)

Abstract

The invention relates to a method for detecting clinically relevant EHEC infections by amplifying both stxA1 and stxA2 sequences in a multiplex amplification reaction. The inventive method is further characterized in that both sequences of human-pathogenic and sequences of porcine-pathogenic stxA2 isoforms are amplified with the primers derived from a specific region of the stxA2 gene.
AU79068/00A 1999-09-28 2000-09-26 Multiplex pcr for detecting ehec infections Abandoned AU7906800A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19946296 1999-09-28
DE19946296A DE19946296A1 (en) 1999-09-28 1999-09-28 Multiplex PCR for the detection of EHEC infections
PCT/EP2000/009356 WO2001023607A2 (en) 1999-09-28 2000-09-26 Multiplex pcr for detecting ehec infections

Publications (1)

Publication Number Publication Date
AU7906800A true AU7906800A (en) 2001-04-30

Family

ID=7923481

Family Applications (1)

Application Number Title Priority Date Filing Date
AU79068/00A Abandoned AU7906800A (en) 1999-09-28 2000-09-26 Multiplex pcr for detecting ehec infections

Country Status (9)

Country Link
EP (1) EP1222312B1 (en)
JP (1) JP2003510092A (en)
AT (1) ATE286145T1 (en)
AU (1) AU7906800A (en)
CA (1) CA2393223A1 (en)
DE (2) DE19946296A1 (en)
ES (1) ES2234680T3 (en)
PT (1) PT1222312E (en)
WO (1) WO2001023607A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020090257A (en) * 2001-05-26 2002-12-02 주식회사 서린바이오사이언스 Multiplex pcr primers for detecting non-pathogenic e.coli, ehec, etec e.coli causing enteritis and o-157:h7, and method for performing multiplex pcr using the same
AT411832B (en) * 2001-07-26 2004-06-25 Sy Lab Vgmbh TEST KIT FOR DETECTING THE BACTERIAL GENES SLT1, SLT2 AND RFBE
JP4571786B2 (en) * 2003-06-10 2010-10-27 則夫 清水 Target nucleic acid detection method
JP4501443B2 (en) 2004-02-03 2010-07-14 東ソー株式会社 Detection reagent for Shiga toxin group gene of enterohemorrhagic Escherichia coli
WO2006076825A1 (en) * 2005-01-18 2006-07-27 Kui Li A method for detecting the pork quality traits and carcass traits
CA2670622C (en) * 2006-11-30 2015-09-15 Id-Fish Technology, Inc. Nucleic acid probes and methods for detecting plasmodium parasites
CN103215364A (en) * 2013-04-26 2013-07-24 镇江盛泰生物医药科技有限公司 Real-time PCR (polymerase chain reaction) detection method of gene point mutation and application of real-time PCR detection method
DE102017210711A1 (en) 2016-06-27 2017-12-28 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Semiconductor device
CN115058528A (en) * 2022-06-17 2022-09-16 湖南大圣宠医生物科技有限公司 Composition, kit and method for detecting and typing pig diarrhea pathogens and application of composition

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
US5652102A (en) * 1994-12-05 1997-07-29 The United States Of America As Represented By The Secretary Of Agriculture Assay for enterohemorrhagic Escherichia coli 0157:H7 by the polymerase chain reaction
ATE260988T1 (en) * 1996-06-04 2004-03-15 Univ Utah Res Found DEVICE AND METHOD FOR CARRYING OUT AND MONITORING POLYMERASE CHAIN REACTIONS
FR2760749B1 (en) * 1997-03-14 1999-04-30 Veterinaires Et Alimentaires C OLIGONUCLEOTIDES DERIVED FROM VERTSOXIN-PRODUCING E. coli VTS GENES, AND USES THEREOF
DE19721825B4 (en) * 1997-05-26 2005-09-22 Karch, Helge, Prof. Dr. Method for the identification of enterohaemorrhagic E. coli

Also Published As

Publication number Publication date
CA2393223A1 (en) 2001-04-05
WO2001023607A2 (en) 2001-04-05
ES2234680T3 (en) 2005-07-01
ATE286145T1 (en) 2005-01-15
JP2003510092A (en) 2003-03-18
PT1222312E (en) 2005-05-31
WO2001023607A3 (en) 2001-12-06
DE19946296A1 (en) 2001-03-29
DE50009139D1 (en) 2005-02-03
EP1222312A2 (en) 2002-07-17
EP1222312B1 (en) 2004-12-29

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase