AU774334B2 - Protein design automation for protein libraries - Google Patents

Protein design automation for protein libraries Download PDF

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Publication number
AU774334B2
AU774334B2 AU11190/00A AU1119000A AU774334B2 AU 774334 B2 AU774334 B2 AU 774334B2 AU 11190/00 A AU11190/00 A AU 11190/00A AU 1119000 A AU1119000 A AU 1119000A AU 774334 B2 AU774334 B2 AU 774334B2
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AU
Australia
Prior art keywords
library
sequences
protein
positions
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU11190/00A
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English (en)
Other versions
AU1119000A (en
Inventor
Jorg Bentzien
Bassil I. Dahiyat
Klaus M. Fiebig
Robert J. Hayes
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Xencor Inc
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Xencor Inc
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Publication date
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Publication of AU1119000A publication Critical patent/AU1119000A/en
Application granted granted Critical
Publication of AU774334B2 publication Critical patent/AU774334B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU11190/00A 1998-10-16 1999-10-15 Protein design automation for protein libraries Ceased AU774334B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10461298P 1998-10-16 1998-10-16
US60/104612 1998-10-16
US15870099P 1999-10-08 1999-10-08
US60/158700 1999-10-08
PCT/US1999/024229 WO2000023564A2 (en) 1998-10-16 1999-10-15 Protein design automation for protein libraries

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2004203224A Division AU2004203224B2 (en) 1998-10-16 2004-07-16 Protein design automation for protein libraries

Publications (2)

Publication Number Publication Date
AU1119000A AU1119000A (en) 2000-05-08
AU774334B2 true AU774334B2 (en) 2004-06-24

Family

ID=26801737

Family Applications (2)

Application Number Title Priority Date Filing Date
AU11190/00A Ceased AU774334B2 (en) 1998-10-16 1999-10-15 Protein design automation for protein libraries
AU2004203224A Ceased AU2004203224B2 (en) 1998-10-16 2004-07-16 Protein design automation for protein libraries

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2004203224A Ceased AU2004203224B2 (en) 1998-10-16 2004-07-16 Protein design automation for protein libraries

Country Status (5)

Country Link
EP (1) EP1157093A1 (ja)
JP (1) JP2003527072A (ja)
AU (2) AU774334B2 (ja)
CA (1) CA2347214A1 (ja)
WO (1) WO2000023564A2 (ja)

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EP0974111B1 (en) 1997-04-11 2003-01-08 California Institute Of Technology Apparatus and method for automated protein design
US7315786B2 (en) 1998-10-16 2008-01-01 Xencor Protein design automation for protein libraries
US20020048772A1 (en) 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US6946265B1 (en) 1999-05-12 2005-09-20 Xencor, Inc. Nucleic acids and proteins with growth hormone activity
US6746853B1 (en) 1999-05-19 2004-06-08 Xencor, Inc. Proteins with insulin-like activity useful in the treatment of diabetes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
EP1621617A1 (en) * 2000-02-10 2006-02-01 Xencor, Inc. Protein design automation for protein libraries
AU5168701A (en) * 2000-02-10 2001-08-20 Xencor Protein design automation for protein libraries
US7662367B2 (en) 2000-03-02 2010-02-16 Xencor, Inc. Pharmaceutical compositions for the treatment of TNF-α related disorders
US7056695B2 (en) 2000-03-02 2006-06-06 Xencor TNF-α variants
US7244823B2 (en) 2000-03-02 2007-07-17 Xencor TNF-alpha variants proteins for the treatment of TNF-alpha related disorders
US7101974B2 (en) 2000-03-02 2006-09-05 Xencor TNF-αvariants
US7446174B2 (en) 2001-03-02 2008-11-04 Xencor, Inc. Protein based TNF-α variants for the treatment of TNF-α related disorders
US7687461B2 (en) 2000-03-02 2010-03-30 Xencor, Inc. Treatment of TNF-α related disorders with TNF-α variant proteins
WO2002004628A2 (en) * 2000-07-06 2002-01-17 Genvec, Inc. Method of identifying a binding partner of a gene product
JP4823470B2 (ja) 2000-07-13 2011-11-24 シーティー−コンセプト・ホールディング・アクチェンゲゼルシャフト 電力半導体スイッチの過渡状態を状態に応じて制御する方法と装置
EP1325457A4 (en) * 2000-10-10 2007-10-24 Genencor Int LIBRARIES RICH IN INFORMATION
US7351690B2 (en) 2000-12-19 2008-04-01 Palatin Technologies, Inc. Knockout identification of target-specific sites in peptides
WO2002068453A2 (en) * 2001-02-22 2002-09-06 Xencor Methods and compositions for the construction and use of fusion libraries using computational protein design methods
JP2005505250A (ja) 2001-05-04 2005-02-24 ゼンコー 核酸およびチオレドキシンレダクターゼ活性を有するタンパク質
US7381792B2 (en) 2002-01-04 2008-06-03 Xencor, Inc. Variants of RANKL protein
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
US7662925B2 (en) 2002-03-01 2010-02-16 Xencor, Inc. Optimized Fc variants and methods for their generation
US7317091B2 (en) 2002-03-01 2008-01-08 Xencor, Inc. Optimized Fc variants
US8188231B2 (en) 2002-09-27 2012-05-29 Xencor, Inc. Optimized FC variants
DE10233047A1 (de) * 2002-07-19 2004-02-26 Amaxa Gmbh Verfahren zur Herstellung eines künstlichen Polypeptids und nach diesem Verfahren hergestelltes künstliches Protein
US20090010920A1 (en) 2003-03-03 2009-01-08 Xencor, Inc. Fc Variants Having Decreased Affinity for FcyRIIb
US8388955B2 (en) 2003-03-03 2013-03-05 Xencor, Inc. Fc variants
US8084582B2 (en) 2003-03-03 2011-12-27 Xencor, Inc. Optimized anti-CD20 monoclonal antibodies having Fc variants
US9051373B2 (en) 2003-05-02 2015-06-09 Xencor, Inc. Optimized Fc variants
US8101720B2 (en) 2004-10-21 2012-01-24 Xencor, Inc. Immunoglobulin insertions, deletions and substitutions
US9714282B2 (en) 2003-09-26 2017-07-25 Xencor, Inc. Optimized Fc variants and methods for their generation
EP1697741A4 (en) 2003-12-04 2008-02-13 Xencor Inc PROCESS FOR PRODUCING PROTEIN VARIANTS WITH INCREASED HOST STRUCTURE CONTENT AND COMPOSITIONS THEREOF
US20150010550A1 (en) 2004-07-15 2015-01-08 Xencor, Inc. OPTIMIZED Fc VARIANTS
US8802820B2 (en) 2004-11-12 2014-08-12 Xencor, Inc. Fc variants with altered binding to FcRn
CN102746404B (zh) 2004-11-12 2016-01-20 赞科股份有限公司 对FcRn的结合被改变的Fc变体
US8546543B2 (en) 2004-11-12 2013-10-01 Xencor, Inc. Fc variants that extend antibody half-life
US8367805B2 (en) 2004-11-12 2013-02-05 Xencor, Inc. Fc variants with altered binding to FcRn
US20090054252A1 (en) * 2005-01-03 2009-02-26 Martin Lanzendoerfer Hemopexin-Like Structure as New Polypeptide-Scaffold
EP1931709B1 (en) 2005-10-03 2016-12-07 Xencor, Inc. Fc variants with optimized fc receptor binding properties
CA2625998C (en) 2005-10-06 2015-12-01 Xencor, Inc. Optimized anti-cd30 antibodies
AU2007285976B2 (en) 2006-08-14 2011-08-18 Xencor, Inc Optimized antibodies that target CD19
CA2660795C (en) 2006-09-18 2014-11-18 Xencor, Inc. Optimized antibodies that target hm1.24
PL2235059T3 (pl) 2007-12-26 2015-08-31 Xencor Inc Warianty FC o zmodyfikowanym wiązaniu do FCRN
JP5703021B2 (ja) 2008-09-26 2015-04-15 日本水産株式会社 脂質の濃縮方法
US9493578B2 (en) 2009-09-02 2016-11-15 Xencor, Inc. Compositions and methods for simultaneous bivalent and monovalent co-engagement of antigens
WO2011091078A2 (en) 2010-01-19 2011-07-28 Xencor, Inc. Antibody fc variants with enhanced complement activity

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1998032845A1 (en) * 1997-01-24 1998-07-30 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0974111B1 (en) * 1997-04-11 2003-01-08 California Institute Of Technology Apparatus and method for automated protein design
JP2002543791A (ja) * 1999-05-12 2002-12-24 ゼンコー 新規な熱安定性の親アルカリ性キシラナーゼ

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1998032845A1 (en) * 1997-01-24 1998-07-30 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PROTEIN SCIENCE, 1996, VOL.5(5), DAHIYAT AND MAYO,PP895-903 *

Also Published As

Publication number Publication date
WO2000023564A3 (en) 2001-12-13
CA2347214A1 (en) 2000-04-27
JP2003527072A (ja) 2003-09-16
EP1157093A1 (en) 2001-11-28
AU1119000A (en) 2000-05-08
AU2004203224B2 (en) 2007-07-05
AU2004203224A1 (en) 2004-08-12
WO2000023564A2 (en) 2000-04-27

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Legal Events

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MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 14, NO 17, PAGE(S) 3249-3254 UNDER THE HEADING APPLICATIONS LAPSED, REFUSED OR WITHDRAWN PLEASE DELETE ALL REFERENCE TO APPLICATION NO. 11190/00

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FGA Letters patent sealed or granted (standard patent)