AU751014B2 - Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents - Google Patents
Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents Download PDFInfo
- Publication number
- AU751014B2 AU751014B2 AU65785/98A AU6578598A AU751014B2 AU 751014 B2 AU751014 B2 AU 751014B2 AU 65785/98 A AU65785/98 A AU 65785/98A AU 6578598 A AU6578598 A AU 6578598A AU 751014 B2 AU751014 B2 AU 751014B2
- Authority
- AU
- Australia
- Prior art keywords
- locus
- expression
- efflux pump
- gene locus
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 121
- 239000000203 mixture Substances 0.000 title claims description 75
- 230000001580 bacterial effect Effects 0.000 title claims description 74
- 239000003960 organic solvent Substances 0.000 title claims description 54
- 239000000645 desinfectant Substances 0.000 title claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 122
- 230000014509 gene expression Effects 0.000 claims description 113
- 239000003795 chemical substances by application Substances 0.000 claims description 102
- 230000003115 biocidal effect Effects 0.000 claims description 73
- 230000001937 non-anti-biotic effect Effects 0.000 claims description 70
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 53
- 230000000844 anti-bacterial effect Effects 0.000 claims description 51
- 241000894006 Bacteria Species 0.000 claims description 40
- 239000003112 inhibitor Substances 0.000 claims description 32
- 150000007523 nucleic acids Chemical class 0.000 claims description 29
- 108020004707 nucleic acids Proteins 0.000 claims description 27
- 102000039446 nucleic acids Human genes 0.000 claims description 27
- 239000003242 anti bacterial agent Substances 0.000 claims description 26
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 claims description 24
- 230000000692 anti-sense effect Effects 0.000 claims description 23
- 230000000694 effects Effects 0.000 claims description 22
- 239000010665 pine oil Substances 0.000 claims description 22
- 230000001965 increasing effect Effects 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- -1 antibodies Proteins 0.000 claims description 15
- 230000002018 overexpression Effects 0.000 claims description 15
- 101150033650 soxS gene Proteins 0.000 claims description 15
- 230000003247 decreasing effect Effects 0.000 claims description 14
- 230000006698 induction Effects 0.000 claims description 14
- 102000004169 proteins and genes Human genes 0.000 claims description 14
- 238000012216 screening Methods 0.000 claims description 13
- 108700005443 Microbial Genes Proteins 0.000 claims description 12
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 10
- 239000003550 marker Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 239000005414 inactive ingredient Substances 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 102000053642 Catalytic RNA Human genes 0.000 claims description 8
- 108090000994 Catalytic RNA Proteins 0.000 claims description 8
- 230000003385 bacteriostatic effect Effects 0.000 claims description 8
- 239000011230 binding agent Substances 0.000 claims description 8
- 239000004067 bulking agent Substances 0.000 claims description 8
- 108091092562 ribozyme Proteins 0.000 claims description 8
- 239000000344 soap Substances 0.000 claims description 8
- 238000013518 transcription Methods 0.000 claims description 8
- 230000035897 transcription Effects 0.000 claims description 8
- OSDLLIBGSJNGJE-UHFFFAOYSA-N 4-chloro-3,5-dimethylphenol Chemical compound CC1=CC(O)=CC(C)=C1Cl OSDLLIBGSJNGJE-UHFFFAOYSA-N 0.000 claims description 7
- 108010077805 Bacterial Proteins Proteins 0.000 claims description 7
- 229960005443 chloroxylenol Drugs 0.000 claims description 7
- 239000006210 lotion Substances 0.000 claims description 7
- 238000009877 rendering Methods 0.000 claims description 7
- 239000003899 bactericide agent Substances 0.000 claims description 6
- 239000000022 bacteriostatic agent Substances 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 5
- 229960004023 minocycline Drugs 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- XEFQLINVKFYRCS-UHFFFAOYSA-N Triclosan Chemical compound OC1=CC(Cl)=CC=C1OC1=CC=C(Cl)C=C1Cl XEFQLINVKFYRCS-UHFFFAOYSA-N 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 4
- 101150066555 lacZ gene Proteins 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 229960003500 triclosan Drugs 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 3
- IVGGLKLMSNTTIX-PMACEKPBSA-N (2S)-2-amino-N-[1-[(2S)-2-amino-5-(diaminomethylideneamino)pentanoyl]naphthalen-2-yl]-3-phenylpropanamide Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)NC2=C(C3=CC=CC=C3C=C2)C(=O)[C@H](CCCN=C(N)N)N)N IVGGLKLMSNTTIX-PMACEKPBSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 230000002596 correlated effect Effects 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims description 2
- DYKFCLLONBREIL-KVUCHLLUSA-N minocycline Chemical compound C([C@H]1C2)C3=C(N(C)C)C=CC(O)=C3C(=O)C1=C(O)[C@@]1(O)[C@@H]2[C@H](N(C)C)C(O)=C(C(N)=O)C1=O DYKFCLLONBREIL-KVUCHLLUSA-N 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000003643 water by type Substances 0.000 claims 1
- 101150008274 marA gene Proteins 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 31
- 229940088710 antibiotic agent Drugs 0.000 description 22
- 230000035772 mutation Effects 0.000 description 22
- 239000013612 plasmid Substances 0.000 description 20
- 108091034117 Oligonucleotide Proteins 0.000 description 17
- 241000588724 Escherichia coli Species 0.000 description 15
- 101150059149 rob gene Proteins 0.000 description 14
- 238000012217 deletion Methods 0.000 description 13
- 230000037430 deletion Effects 0.000 description 13
- 239000012634 fragment Substances 0.000 description 12
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
- 230000002759 chromosomal effect Effects 0.000 description 11
- 101150114554 marR gene Proteins 0.000 description 11
- 239000008186 active pharmaceutical agent Substances 0.000 description 9
- 239000000074 antisense oligonucleotide Substances 0.000 description 9
- 238000012230 antisense oligonucleotides Methods 0.000 description 9
- 238000009396 hybridization Methods 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 239000004480 active ingredient Substances 0.000 description 8
- 239000004098 Tetracycline Substances 0.000 description 7
- 101150079343 acrR gene Proteins 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 229960002180 tetracycline Drugs 0.000 description 7
- 229930101283 tetracycline Natural products 0.000 description 7
- 235000019364 tetracycline Nutrition 0.000 description 7
- 150000003522 tetracyclines Chemical class 0.000 description 7
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 6
- 239000006142 Luria-Bertani Agar Substances 0.000 description 6
- 229960005091 chloramphenicol Drugs 0.000 description 6
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 6
- 230000002779 inactivation Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 101150001675 APS3 gene Proteins 0.000 description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- 229960000318 kanamycin Drugs 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 229930182823 kanamycin A Natural products 0.000 description 5
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 5
- 229960000210 nalidixic acid Drugs 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 241000600169 Maro Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 238000000636 Northern blotting Methods 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 239000004599 antimicrobial Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000007899 nucleic acid hybridization Methods 0.000 description 4
- 230000004962 physiological condition Effects 0.000 description 4
- 230000003389 potentiating effect Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- FFTVPQUHLQBXQZ-KVUCHLLUSA-N (4s,4as,5ar,12ar)-4,7-bis(dimethylamino)-1,10,11,12a-tetrahydroxy-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1C2=C(N(C)C)C=CC(O)=C2C(O)=C2[C@@H]1C[C@H]1[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]1(O)C2=O FFTVPQUHLQBXQZ-KVUCHLLUSA-N 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 108700005075 Regulator Genes Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000411 inducer Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 230000026683 transduction Effects 0.000 description 3
- 238000010361 transduction Methods 0.000 description 3
- 108091027075 5S-rRNA precursor Proteins 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 101100394050 Escherichia coli (strain K12) gyrB gene Proteins 0.000 description 2
- 241001646716 Escherichia coli K-12 Species 0.000 description 2
- 108010008177 Fd immunoglobulins Proteins 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000006137 Luria-Bertani broth Substances 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 206010034133 Pathogen resistance Diseases 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 101150004068 acrB gene Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000002814 agar dilution Methods 0.000 description 2
- 150000001412 amines Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- JQXXHWHPUNPDRT-BQVAUQFYSA-N chembl1523493 Chemical compound O([C@](C1=O)(C)O\C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)/C=C\C=C(C)/C(=O)NC=2C(O)=C3C(O)=C4C)C)OC)C4=C1C3=C(O)C=2C=NN1CCN(C)CC1 JQXXHWHPUNPDRT-BQVAUQFYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 229940124307 fluoroquinolone Drugs 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- QOIGKGMMAGJZNZ-UHFFFAOYSA-N gepirone Chemical compound O=C1CC(C)(C)CC(=O)N1CCCCN1CCN(C=2N=CC=CN=2)CC1 QOIGKGMMAGJZNZ-UHFFFAOYSA-N 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 2
- 150000004713 phosphodiesters Chemical class 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229960001225 rifampicin Drugs 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000011269 treatment regimen Methods 0.000 description 2
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical class CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 108010032595 Antibody Binding Sites Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 101150038588 CHRNB1 gene Proteins 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241001480079 Corymbia calophylla Species 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 101001077660 Homo sapiens Serine protease inhibitor Kazal-type 1 Proteins 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 201000008225 Klebsiella pneumonia Diseases 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- LTGPFZWZZNUIIK-LURJTMIESA-N Lysol Chemical compound NCCCC[C@H](N)CO LTGPFZWZZNUIIK-LURJTMIESA-N 0.000 description 1
- 239000006154 MacConkey agar Substances 0.000 description 1
- 206010048723 Multiple-drug resistance Diseases 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 206010035717 Pneumonia klebsiella Diseases 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 101100137512 Proteus vulgaris pqrA gene Proteins 0.000 description 1
- 241000588768 Providencia Species 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 108010034634 Repressor Proteins Proteins 0.000 description 1
- 102000009661 Repressor Proteins Human genes 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 102100025144 Serine protease inhibitor Kazal-type 1 Human genes 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 125000005600 alkyl phosphonate group Chemical group 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- XKXHCNPAFAXVRZ-UHFFFAOYSA-N benzylazanium;chloride Chemical compound [Cl-].[NH3+]CC1=CC=CC=C1 XKXHCNPAFAXVRZ-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000024203 complement activation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-N dithiophosphoric acid Chemical class OP(O)(S)=S NAGJZTKCGNOGPW-UHFFFAOYSA-N 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 101150069102 marB gene Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 231100000150 mutagenicity / genotoxicity testing Toxicity 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 230000006506 pH homeostasis Effects 0.000 description 1
- 150000002960 penicillins Chemical class 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000008298 phosphoramidates Chemical class 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229940007042 proteus vulgaris Drugs 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 150000007660 quinolones Chemical class 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 229960001860 salicylate Drugs 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229960004025 sodium salicylate Drugs 0.000 description 1
- 101150112901 soxA gene Proteins 0.000 description 1
- 101150100021 soxR gene Proteins 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
- C07K5/06008—Dipeptides with the first amino acid being neutral
- C07K5/06078—Dipeptides with the first amino acid being neutral and aromatic or cycloaliphatic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Communicable Diseases (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Oncology (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6089897P | 1997-10-03 | 1997-10-03 | |
| US60/060898 | 1997-10-03 | ||
| PCT/US1998/005671 WO1999017791A1 (en) | 1997-10-03 | 1998-03-20 | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU48950/02A Division AU4895002A (en) | 1997-10-03 | 2002-06-25 | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU6578598A AU6578598A (en) | 1999-04-27 |
| AU751014B2 true AU751014B2 (en) | 2002-08-08 |
Family
ID=22032436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU65785/98A Ceased AU751014B2 (en) | 1997-10-03 | 1998-03-20 | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US6068972A (enExample) |
| EP (1) | EP1019076A4 (enExample) |
| JP (1) | JP2001518514A (enExample) |
| AU (1) | AU751014B2 (enExample) |
| CA (1) | CA2305528A1 (enExample) |
| WO (1) | WO1999017791A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1077718B1 (en) * | 1998-05-18 | 2002-08-14 | PHARMACIA & UPJOHN COMPANY | Enhancement of oxazolidinone antibacterial agents activity by arginine derivatives |
| US6346391B1 (en) | 1999-07-22 | 2002-02-12 | Trustees Of Tufts College | Methods of reducing microbial resistance to drugs |
| US20020132798A1 (en) | 2000-06-16 | 2002-09-19 | Nelson Mark L. | 7-phenyl-substituted tetracycline compounds |
| WO2002016940A2 (en) * | 2000-08-23 | 2002-02-28 | Genome Therapeutics Corporation | Genomics-assisted rapid identification of targets |
| US7713472B2 (en) * | 2001-01-12 | 2010-05-11 | Board Of Regents, The University Of Texas System | Antiseptic derivatives with broad spectrum antimicrobial activity for the impregnation of surfaces |
| US7075582B2 (en) | 2001-07-13 | 2006-07-11 | Paratek Pharmaceuticals, Inc. | Methods for identifying and using MarR family polypeptide binding compounds |
| WO2003006626A2 (en) * | 2001-07-13 | 2003-01-23 | Trustees Of Tufts College | CRYSTAL STRUCTURE OF A MarR FAMILY POLYPEPTIDE |
| US20060084678A1 (en) * | 2001-07-13 | 2006-04-20 | Paratek Pharmaceuticals, Inc. | Methods for identifying and using MarR family polypeptide binding compounds |
| US20050255538A1 (en) * | 2002-03-15 | 2005-11-17 | Buxser Stephen E | Methods for screening for AcrAB efflux pump inhibitors |
| WO2004062674A2 (en) * | 2003-01-07 | 2004-07-29 | Paratek Pharmaceuticals, Inc. | Substituted polyamines as inhibitors of bacterial efflux pumps |
| PL1644024T3 (pl) | 2003-06-06 | 2020-03-31 | Board Of Regents, The University Of Texas System | Przeciwdrobnoustrojowe roztwory do płukania |
| US7652040B2 (en) * | 2003-06-19 | 2010-01-26 | Janssen Pharmaceutica N.V. | Aminosulfonyl substituted 4-(aminomethyl)-piperidine benzamides as 5HT4-antagonists |
| JP2007522835A (ja) * | 2004-01-20 | 2007-08-16 | ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム | 医療装置に消毒組成物をコーティングする、および含浸させる方法 |
| US20070154621A1 (en) | 2005-11-18 | 2007-07-05 | Issam Raad | Methods for coating surfaces with antimicrobial agents |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW318189B (enExample) * | 1991-12-06 | 1997-10-21 | American Cyanamid Co | |
| US5384259A (en) * | 1991-12-06 | 1995-01-24 | American Cyanamid Company | Construct and method for expression of tetracycline resistance genes in E. Coli |
| EP0658213B1 (en) * | 1992-08-28 | 1998-11-18 | Trustees Of Tufts College | Multiple antibiotic resistance operon assays |
| US5989832A (en) * | 1995-04-21 | 1999-11-23 | Microcide Pharmaceuticals, Inc. | Method for screening for non-tetracycline efflux pump inhibitors |
-
1997
- 1997-10-07 US US08/946,225 patent/US6068972A/en not_active Expired - Lifetime
-
1998
- 1998-03-20 JP JP2000514662A patent/JP2001518514A/ja active Pending
- 1998-03-20 EP EP98911949A patent/EP1019076A4/en not_active Withdrawn
- 1998-03-20 WO PCT/US1998/005671 patent/WO1999017791A1/en not_active Ceased
- 1998-03-20 CA CA002305528A patent/CA2305528A1/en not_active Abandoned
- 1998-03-20 AU AU65785/98A patent/AU751014B2/en not_active Ceased
-
2000
- 2000-02-29 US US09/515,705 patent/US6448006B1/en not_active Expired - Fee Related
-
2002
- 2002-07-24 US US10/205,591 patent/US20030171317A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US6448006B1 (en) | 2002-09-10 |
| EP1019076A4 (en) | 2002-01-23 |
| JP2001518514A (ja) | 2001-10-16 |
| US6068972A (en) | 2000-05-30 |
| WO1999017791A1 (en) | 1999-04-15 |
| CA2305528A1 (en) | 1999-04-15 |
| US20030171317A1 (en) | 2003-09-11 |
| EP1019076A1 (en) | 2000-07-19 |
| AU6578598A (en) | 1999-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU751014B2 (en) | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents | |
| Moken et al. | Selection of multiple-antibiotic-resistant (mar) mutants of Escherichia coli by using the disinfectant pine oil: roles of the mar and acrAB loci | |
| US6924101B2 (en) | Methods for identifying anti-microbial agents | |
| Geiger et al. | Role of the (p) ppGpp synthase RSH, a RelA/SpoT homolog, in stringent response and virulence of Staphylococcus aureus | |
| Martínez‐Bueno et al. | Analysis of the gene cluster involved in production and immunity of the peptide antibiotic AS‐48 in Enterococcus faecalis | |
| Fernández et al. | Adaptive resistance to the “last hope” antibiotics polymyxin B and colistin in Pseudomonas aeruginosa is mediated by the novel two-component regulatory system ParR-ParS | |
| Mathur et al. | Antimicrobial peptides activate the Vibrio choleraeσE regulon through an OmpU‐dependent signalling pathway | |
| Molina et al. | A two‐partner secretion system is involved in seed and root colonization and iron uptake by Pseudomonas putida KT2440 | |
| Sass et al. | The lantibiotic mersacidin is a strong inducer of the cell wall stress response of Staphylococcus aureus | |
| WO2002079467A2 (en) | Antibiotic-free bacterial strain selection with antisense molecules | |
| JP2001523097A (ja) | ａａｒＣ及びその組成物を利用する抗菌剤のスクリーニング方法 | |
| WO1999052563A9 (en) | A method for the targeting of proteins produced by yersinia into the cytosol of eukaryotic cells | |
| Cho et al. | Involvement of lipoprotein P pi A of S treptococcus gordonii in evasion of phagocytosis by macrophages | |
| WO1999017607A2 (en) | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents | |
| Patel et al. | Host protein interactions with enteropathogenic Escherichia coli (EPEC): 14‐3‐3τ binds Tir and has a role in EPEC‐induced actin polymerization | |
| AU4895002A (en) | Methods and compositions for reducing bacterial tolerance of disinfectants and organic solvents | |
| WO1997040160A1 (en) | A prokaryotic protein having functional and structural homology with the human p-glycoprotein encoded by the mdr-1 gene, nucleic acids encoding and cells expressing said protein | |
| Thomsen et al. | The heme sensing response regulator HssR in Staphylococcus aureus but not the homologous RR23 in Listeria monocytogenes modulates susceptibility to the antimicrobial peptide plectasin | |
| AU777774B2 (en) | Novel method for identifying antibacterial compounds | |
| Scupham et al. | Determination of the amino acid residues required for the activity of the anti‐rhizobial peptide antibiotic trifolitoxin | |
| Arias et al. | Role of the transmembrane domain of the VanT serine racemase in resistance to vancomycin in Enterococcus gallinarum BM4174 | |
| Küster et al. | Cloning and transcriptional analysis of the rplKA-or f31-rplJL gene cluster of Streptomyces griseus | |
| EP1355927A2 (en) | Identification of the anti-sigma-28 factor in helicobacter pylori, in campylobacter jejuni and in pseudomonas aeruginosa and application thereof | |
| Rodriguez-Martinez et al. | O261 Role of the pentapeptidic proteins from Gram-positive bacteria as a possible source of Qnr-like quinolone resistance determinants | |
| De Boever | Genetic and transcriptional analyses of the pheromone response of the Enterococcus faecalis plasmid pAM373 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |