AU740718B2 - Polynucleotide separations on nonporous polymer beads - Google Patents

Polynucleotide separations on nonporous polymer beads Download PDF

Info

Publication number
AU740718B2
AU740718B2 AU71620/98A AU7162098A AU740718B2 AU 740718 B2 AU740718 B2 AU 740718B2 AU 71620/98 A AU71620/98 A AU 71620/98A AU 7162098 A AU7162098 A AU 7162098A AU 740718 B2 AU740718 B2 AU 740718B2
Authority
AU
Australia
Prior art keywords
beads
bead
dna
polymer
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU71620/98A
Other languages
English (en)
Other versions
AU7162098A (en
Inventor
Douglas T. Gjerde
Robert M. Haefele
Paul D. Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of AU7162098A publication Critical patent/AU7162098A/en
Application granted granted Critical
Publication of AU740718B2 publication Critical patent/AU740718B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/366—Ion-pair, e.g. ion-pair reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/327—Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU71620/98A 1997-04-25 1998-04-23 Polynucleotide separations on nonporous polymer beads Ceased AU740718B2 (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US4485697P 1997-04-25 1997-04-25
US60/044856 1997-04-25
US5963697P 1997-09-23 1997-09-23
US60/059636 1997-09-23
US6232197P 1997-10-17 1997-10-17
US60/062321 1997-10-17
US6362897P 1997-10-27 1997-10-27
US60/063628 1997-10-27
US6767997P 1997-12-05 1997-12-05
US60/067679 1997-12-05
US7787598P 1998-03-13 1998-03-13
US60/077875 1998-03-13
US5858098A 1998-04-10 1998-04-10
US09/058580 1998-04-10
PCT/US1998/008388 WO1998048913A1 (en) 1997-04-25 1998-04-23 Polynucleotide separations on nonporous polymer beads

Publications (2)

Publication Number Publication Date
AU7162098A AU7162098A (en) 1998-11-24
AU740718B2 true AU740718B2 (en) 2001-11-15

Family

ID=27567975

Family Applications (1)

Application Number Title Priority Date Filing Date
AU71620/98A Ceased AU740718B2 (en) 1997-04-25 1998-04-23 Polynucleotide separations on nonporous polymer beads

Country Status (5)

Country Link
EP (1) EP1017466A4 (enExample)
JP (1) JP2002506426A (enExample)
AU (1) AU740718B2 (enExample)
CA (1) CA2286350C (enExample)
WO (1) WO1998048913A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6699698B1 (en) 1999-02-15 2004-03-02 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Method and sample mount system for the in situ separation and enrichment of substances
US7169298B2 (en) 2000-01-26 2007-01-30 Transgenomic, Inc. Method and apparatus for separating polynucleotides using monolithic capillary columns
AU2001227653A1 (en) * 2000-03-09 2001-09-17 Transgenomic, Inc. Method and system for rna analysis by matched ion polynucleotide chromatography
JP2003534798A (ja) * 2000-05-26 2003-11-25 トランスジエノミツク・インコーポレーテツド ポリヌクレオチドを分離しそして精製するための装置及び方法
US6521411B2 (en) * 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
WO2003031580A2 (en) 2001-10-05 2003-04-17 Transgenomic, Inc. Methods and compositions for mutation analysis by liquid chromatography
EP2610296A1 (de) 2011-12-29 2013-07-03 Lanxess Deutschland GmbH Verfahren zur Herstellung von gereinigten Nitrilkautschuken
JP6342328B2 (ja) 2012-07-23 2018-06-13 株式会社ダイセル 固定相
CN114539458B (zh) * 2020-11-26 2023-07-25 西安蓝晓科技新材料股份有限公司 一种应用于固相合成的多孔树脂及其制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585236A (en) * 1992-11-18 1996-12-17 Sarasep, Inc. Nucleic acid separation on alkylated nonporous polymer beads

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585236A (en) * 1992-11-18 1996-12-17 Sarasep, Inc. Nucleic acid separation on alkylated nonporous polymer beads

Also Published As

Publication number Publication date
EP1017466A4 (en) 2001-07-11
CA2286350C (en) 2005-02-22
EP1017466A1 (en) 2000-07-12
CA2286350A1 (en) 1998-11-05
WO1998048913A1 (en) 1998-11-05
AU7162098A (en) 1998-11-24
JP2002506426A (ja) 2002-02-26

Similar Documents

Publication Publication Date Title
JP3395906B2 (ja) 核酸断片の分離法
US6066258A (en) Polynucleotide separations on polymeric separation media
JP4593785B2 (ja) 逆相イオン対高速液体クロマトグラフィーにより核酸を分離するためのモノリシックマトリックス
CA2286350C (en) Polynucleotide separations on nonporous polymer beads
US6652745B2 (en) Column for DNA separation by matched ion polynucleotide chromatography
Hosoya et al. In situ surface-selective modification of uniform size macroporous polymer particles with temperature-responsive poly-N-isopropylacrylamide
US5986085A (en) Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
JP2000516638A (ja) 液体クロマトグラフィーを使用するポリヌクレオチド分離を実施するための系および方法
US6355791B1 (en) Polynucleotide separations on polymeric separation media
US6576133B2 (en) Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) Process for separation of polynucleotide fragments
AU777251B2 (en) Polynucleotide separations on polymeric separation media
US6482317B2 (en) Polynucleotide separations on polymeric separation media
EP1231993A1 (en) Improved column for dna separation by matched ion polynucleotide chromatography
US20010051715A1 (en) Chromatographic method for RNA stabilization
WO2001066218A1 (en) Chromatographic method for rna stabilization
WO2001066216A1 (en) Method and system for rna analysis by matched ion polynucleotide chromatography
US7138518B1 (en) Liquid chromatographic separation of polynucleotides

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)