AU723413B2 - An interleukin-5 antagonist - Google Patents

Description

P:\OPERVMROU27594-97 spe dc-23 It=, 2O(X) -1- AN INTERLEUKIN-5 ANTAGONIST The present invention relates to modified and variant forms of Interleukin-5 molecules capable of antagonizing or reducing the activity of IL-5 and their use in ameliorating, abating or otherwise reducing the aberrant effects caused by native or mutant forms of Sequence Identity Numbers (SEQ ID NOs.) for the nucleotide and amino acid sequences referred to in the specification are defined following the description.

Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be ounderstood to imply the inclusion of a stated integer or step or group of integers or steps but see not the exclusion of any other integer or step or group of integers or steps.

15 The reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that that prior art forms part of the common general knowledge in Australia.

e* The rapidly increasing sophistication of recombinant DNA technology is greatly facilitating

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20 research and development in the medical and allied health fields. This is particularly S important in the area of haemopoietic growth factor research where a number of disease conditions are predicated on the aberrant effects of native or mutant forms of growth factors.

One particularly important haemopoietic growth factor is IL-5. This molecule is a lymphokine S 25 secreted by T-cells and mast cells and is a disulfide-linked homodimeric glycoprotein. The human form of this molecule comprises 114 amino acids per monomer. IL-5 consists of a bundle of four a-helices in an up-up, down-down array. The phenomenon of D-helix swapping whereby one bundle is built up of three helices coming from one monomer and a fourth helix which is contributed by the second monomer is unique to IL-5. The IL-5 molecule also contains two short anti-parallel P-strands located between helices A and B and helices C S\and D.

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5 0 0 0e 0 0 5* WO 97/45448 PCT/AU97/00322 -2- Human IL-5 binds to the a-subunit but the binding affinity is increased upon association with the P-chain. The P-chain is shared by other cytokines such as GM-CSF and IL-3.

is a haemopoietic growth factor with selectivity for production and activation of human eosinophils. There is a need, therefore, to develop antagonists of IL-5 to act as therapeutic agents for chronic asthma or other disease states with demonstrated eosinophilia or other conditions associated with IL-5. It is also important for the IL-5 antagonist not to interfere with the activities of other cytokines, such as GM-CSF or IL-3.

Accordingly, one aspect of the present invention contemplates a modified IL-5 comprising a sequence of amino acids within a first a-helix wherein one or more exposed amino acids in said first a-helix having acidic or acidic-like properties are substituted with a basic amino acid residue or a non-acidic amino acid residue.

The IL-5 which is subject to modification is generally of mammalian origin such as from humans, primates, livestock animals (eg. sheep, cows, pigs, horses), laboratory test animals (eg. mice, rats, guinea pigs, rabbits), companion animals (eg. dogs, cats) and captive wild animals (eg. kangaroos, foxes, deer). Most preferably, the IL-5 is of human origin. The modified IL-5 of the present invention may be glycosylated or unglycosylated and does not interfere with GM-CSF or IL-3 activity.

Even more particularly, the present invention is directed to a modified human IL-5 molecule comprising a sequence of amino acids wherein Glu at amino acid position 13 (or its equivalent position) in a first a-helix is replaced by Arg or Lys or a chemical equivalent or derivative thereof. An alternative substitution may also be made using non-acidic amino acid residues such as but not limited to Gin and Asn or their chemical equivalent or derivatives. A "derivative" may be a naturally occurring or synthetic amino acid residue.

In accordance with the present invention, it is proposed that the modified IL-5 molecules defined above act as antagonists of the native form of IL-5. The term "modified" is WO 97/45448 PCT/AU97/00322 -3considered herein synonymous with terms such as "variant", "derivative" and "mutant". The present invention is particularly directed to a modified IL-5 which exhibits specific antagonism of IL-5 mitogenic effects such as observed in vitro. The modified molecules may be glycosylated or unglycosylated and do not interfere with GM-CSF or IL- 3 activity.

Accordingly, another aspect of the present invention is directed to an IL-5 antagonist said antagonist comprising an IL-5 molecule having an amino acid sequence in its first a-helix wherein one or more exposed amino acids in said first a-helix having acidic or acidic-like properties are substituted with a basic amino acid residue or a non-acidic amino acid residue.

More particularly, the present invention provides an antagonist of IL-5 said antagonist comprising an IL-5 molecule with Gin at position 13 (or its equivalent position) in a first ahelix substituted by Arg or Lys or a chemical equivalent or derivative thereof. An alternatively substitution may also be made using non-acidic amino acid residues such as but not limited to Gin and Asn or their chemical equivalents or derivatives.

The modified IL-5 molecule of the present invention is preferably in recombinant or synthetic form and, with the exception of the amino acid substitution(s) in the first a-helix, the amino acid sequence of the IL-5 may be the same as the naturally occurring molecule native molecule) or may carry single or multiple amino acid substitutions, deletions and/or additions to the native amino acid sequence. It is then referred to as a "mutant" The structure of the first a-helix of iL-5 has been determined at 2.4 angstrom resolution by X-ray crystallography and comprises amino acid residues 7 to 27 or their equivalents (see Milburn et al. Nature 363: 172-176, 1993). The modified IL-5 of the present invention may or may not comprise a leader sequence.

The nucleotide and corresponding amino acid sequence for the modified IL-5 having Arg in position 13 is shown in SEQ ID NOs: 1 and 2 and Figure 1. The leader sequence is shown WO 97/45448 PCT/AU97/00322 -4as amino acids 1 to 6 (Met His Tyr His His His [SEQ ID Consequently, amino acids 7 to 27 are shown as amino acids 13-33 in SEQ ID NOs: 1 and 2 and in Figure 1.

Reference to amino acids 7 to 27 is taken as amino acid residue numbers in a molecule without a leader sequence. The amino acid sequence for amino acids 7 to 27 is shown as SEQ ID NO:4 except that amino acid 13 is represented as Xaa. In accordance with the present invention Xaa is preferably a basic amino acid residue or a non-acidic amino acid residue.

Reference to "unglycosylated form" herein means that the molecule is completely unglycosylated such as when expressed in recombinant form in a prokaryotic organism (e.g.

E. coli). Alternatively, a glycosylation-deficient mammalian cell may be used or complete deglycosylation may occur in vitro using appropriate enzymes. Different glycosylation patterns are encompassed by the present invention such as when recombinant molecules are produced in different mammalian cells.

An "exposed" amino acid is taken herein to refer to an amino acid on a solvent-exposed or outer portion of an a-helix compared to those amino acids orientated towards the inside of the molecule.

An acidic amino acid includes, for example, Glu and Asp. Preferred basic amino acids are Arg and Lys. Preferred non-acidic amino acids are Gin and Asn.

According to characterised another aspect of the present invention there is provided a modified by: comprising a sequence of amino acids within a first a-helix; (ii) having one or more exposed amino acids in said a-helix which have acidic or acidic-like properties being substituted by a basic amino acid residue or a non-acidic amino acid residue; (iii) being in recombinant or synthetic form; and (iv) being capable of antagonising IL-5 mitogenic activity in vitro.

WO 97/45448 PCT/AU97/00322 In a related embodiment, the present invention provides an IL-5 antagonist characterised by: comprising a sequence of amino acids within a first a-helix; (ii) having one or more exposed amino acids in said a-helix which have acidic or acidic-like properties being substituted by a basic amino acid residue or a non-acidic amino acid residue; (iii) being in recombinant or synthetic form; and (iv) being capable of antagonising IL-5 mitogenic activity in vitro.

The IL-5 mutant may be in glycosylated or unglycosylated form.

This aspect of the present invention is predicated in part on the finding that a mutation in amino acid 13 (Glu) of human IL-5 to Arg results in the hIL-5 variant being capable of antagonising IL-5 mitogenic effects in vitro. This variant is referred to herein as Arg"" or "E13 Such a variant would be unable to bind to high affinity receptors but would still able to fully bind the low affinity a chain of the IL-5 receptor. Importantly, the IL-5 Arg 1 3 mutant acts as an antagonist, preventing the stimulatory effect of native IL- A particularly important use of the IL-5 Arg 1 3 antagonist is in reducing or otherwise antagonising IL-5-mediated stimulation and activation of eosinophils in vivo or in vitro.

The antagonist may also be able to antagonise effects caused by a mutated, endogenous IL-5. The present invention extends to a range of other IL-5 mutants such as IL-5 Lys 3 Gin 1 3 and IL-5 Asn 1 3 as well as functionally equivalent mutants. The nucleotide and corresponding amino acid sequence for IL-5 Arg 1 3 (E13R) are shown in SEQ ID NOs: 1 and 2. The present invention extends, in a particularly preferred embodiment, to an isolated polypeptide having an amino acid sequence substantially as set forth in SEQ ID NO:2 or a genetic sequence encoding same having a nucleotide sequence substantially as set forth in SEQ ID NO:1.

By way of a shorthand notation, both single and three letter abbreviations are used for amino acid residues in the subject specification and these are defined in Table 1.

WO 97/45448 PCT/AU97/00322 -6- Where a specific amino residue is referred to by its position in IL-5, a single or three letter amino acid abbreviation is used with the residue number given in superscript (eg.

Xaa", wherein Xaa is the amino acid residue) and is the residue number in the molecule.

WO 97/45448 PCT/AU97/00322 -7- Table 1 Amino acid Three-letter Corresponding abbreviation single-letter abbreviation Alanine Ala A Arginine Arg R Asparagine Asn N Aspartic acid Asp D Cysteine Cys C Glutamine Gin Q Glutamic acid Glu E Glycine Gly G Histidine His H Isoleucine lie I Leucine Leu L Lysine Lys K Methionine Met M Phenylalanine Phe F Proline Pro P Serine Ser S Threonine Thr T Tryptophan Trp W Tyrosine Tyr Y Valine Val V The present invention is exemplified using IL-5 Arg 3 This is done, however, with the understanding that the present invention extends to all other IL-5 variants having antagonistic properties to IL-5 in vitro or against eosinophils in vitro or in vivo.

WO 97/45448 PCT/AU97/00322 -8- According to another aspect of the present invention there is provided an IL-5 variant comprising an amino acid sequence in the first a-helix of said Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu lie Ala [SEQ ID No. 4]; wherein Xaa is a basic or non-acidic amino acid residue preferably selected from the group consisting of Arg, Lys, Gin and Asn and wherein said variant IL-5 acts as an antagonist for at least one property of the corresponding native IL-5. The amino acid sequence defined by SEQ ID NO:4 corresponds to amino acid residues 7 to 27 of human IL-5. Preferably, Xaa is Xaa" wherein n is amino acid position 13 of human IL-5. Preferably Xaa" is Arg 1 3 or its equivalent.

In a related embodiment, the present invention contemplates an IL-5 antagonist comprising a polypeptide or chemical equivalent thereof comprising amino acids 7 to 27 of the first ahelix of human IL-5 with the proviso that one or more exposed amino acids in said first ahelix having acidic or acidic-like properties are substituted by a basic amino acid residue or a non-acidic amino acid residue.

Preferably, the acidic or acidic-like amino acid residue if Glu or Asp and is replaced by Arg, Lys, Gin or Asn.

More preferably, the acidic or acidic-like amino acid residue is replaced by Arg.

Most preferably, the amino acid sequence of the modified IL-5 is as set forth in SEQ ID NO:2.

In a particularly preferred embodiment, the present invention is directed to an antagonist comprising a modified IL-5 molecule having the following amino acid sequence in its first a-helix: WO 97/45448 PCT/AU97/00322 -9- Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO:4] wherein Xaa is Arg; or an IL-5 molecule having a single or multiple mutation in its first a-helix giving functionally similar antagonistic properties to the mutation wherein Xaa is Arg.

In respect of the latter embodiment, the mutation in the IL-5 molecule may be a single or multiple amino acid substitution, deletion and/or addition or may be an altered glycosylation pattern amongst other mutations. Preferably, the IL-5 antagonist comprises an amino acid sequence as set forth in SEQ ID NO:2.

The IL-5 antagonists of the present invention and in particular IL-5 Arg 1 3 are useful inter alia in the treatment of allergy, some myeloid leukemias (such as eosinophilic myeloid leukaemia), idiopathic eosinophilic syndrome, allergic inflammations such as asthma, rhinitis and skin allergies by preventing or reducing IL-5-mediated activation of eosinophils.

These and other conditions are considered herein to result from or be facilitated by the aberrant effects of an endogenous native IL-5 or an endogenous naturally mutated The present invention, therefore, contemplates a method of treatment comprising the administration to a mammal of an effective amount of a modified IL-5 as hereinbefore defined and in particular IL-5 Arg" 3 for a time and under conditions sufficient for effecting said treatment.

Preferably, the treatment is in respect of the treatment of allergy, some myeloid leukemias (such as eosinophilic myeloid leukaemia), idiopathic eosinophilic syndrome, allergic inflammations such as asthma, rhinitis andskin allergies.

Generally, the mammal to be treated is a human, primate, livestock animal, companion animal or laboratory test animal. Most preferably, the mammal is a human.

WO 97/45448 PCT/AU97/00322 A single modified IL-5 may be administered or a combination of variants of the same For example, a range of IL-5 variants could be used such as a combination selected from two or more of IL-5 Arg 1 3 IL-5 Lys' 3 IL-5 Gin 13 and IL-5 Asn 1 3 The IL-5 variants of the present invention are particularly useful in treating eosinophilia and conditions resulting therefrom such as asthma. Administration is preferably by intravenous administration but a range of other forms of administration are contemplated by the present invention including via an implant device or other form allowing sustained release of the IL-5 variant, in a nebuliser form or nasal spray. Modified forms of IL-5 permit entry following topical application are also encompassed by the present invention.

In addition to the modifications to IL-5 contemplated above, the present invention further provides a range of other derivatives of Such derivatives include fragments, parts, portions, mutants, homologues and analogues of the IL-5 polypeptide and corresponding genetic sequence. Derivatives also include single or multiple amino acid substitutions, deletions and/or additions to IL-5 or single or multiple nucleotide substitutions, deletions and/or additions to the genetic sequence encoding Derivatives also contemplated modifications to resident nucleotides. Alteration of the nucleotides may result in a corresponding altered amino acid sequence or altered glycosylation patterns amongst other effects. "Additions" to amino acid sequences or nucleotide sequences include fusions with other peptides, polypeptides or proteins or fusions to nucleotide sequences. Reference herein to "IL-5" includes reference to all derivatives thereof including functional derivatives or IL-5 immunologically interactive derivatives.

All such derivatives would be in addition to the modifications to the first a-helix contemplated above. Accordingly, such derivatives would be to IL-5 Arg 1 3 IL-5 Lys 13

IL-

Gln" 1 or IL-5 Asn 1 3 Analogues of IL-5 contemplated herein include, but are not limited to, modification to side chains, incorporating of unnatural amino acids and/or their derivatives during peptide, polypeptide or protein synthesis and the use of crosslinkers and other methods which impose WO 97/45448 PCT/AU97/00322 11 conformational constraints on the proteinaceous molecule or their analogues.

The present invention further contemplates chemical analogues of IL-5 capable of acting as antagonists or agonists of IL-5 or which can act as functional analogues of IL-5. Chemical analogues may not necessarily be derived from IL-5 but may share certain conformational similarities. Alternatively, chemical analogues may be specifically designed to mimic certain physiochemical properties of IL-5. Chemical analogues may be chemically synthesised or may be detected following, for example, natural product screening.

Other derivatives contemplated by the present invention include a range of glycosylation variants from a completely unglycosylated molecule to a fully glycosylated molecule and from a naturally glycosylated molecule to molecules with an altered glycosylation pattern.

Altered glycosylation patterns may result from expression of recombinant molecules in different host cells.

All these types of modifications may be important to stabilise the modified IL-5 molecules if administered to an individual or when used as a diagnostic reagent. The modifications may also add, complement or otherwise facilitate the antagonistic properties of the modified molecules.

Reference herein to a "modified" IL-5, therefore, includes reference to an IL-5 with an altered amino acid sequence in the first a-helix as well as, where appropriate, a range of glycosylation variants, amino acid variations in other parts of the molecule, chemical modifications to the molecule as well as fusion molecules.

The present invention also provides a pharmaceutical composition comprising the modified molecules as hereinbefore defined or combinations thereof.

Accordingly, the present invention contemplates a pharmaceutical composition comprising a a modified IL-5, said modified IL-5 comprising a sequence of amino acids with a first a- WO 97/45448 PCT/AU97/00322 12helix wherein one or more exposed amino acids in said first a-helix having acidic or acidiclike properties are substituted with a basic amino acid residue or non-acidic amino acid residue, said composition further comprising one or more pharmaceutically acceptable carriers and/or diluents.

In a related embodiment, the present invention provides a pharmaceutical composition comprising a modified human IL-5 or a mammalian homologue thereof said modified comprising a sequence of amino acids in a first a-helix of: Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO:4] wherein Xaa is a basic non-acidic amino acid residue preferably selected from Arg, Lys, Gin and Asn, said composition further comprising one or more pharmaceutically acceptable carriers and/or diluents. Preferably, Xaa is Xaa n where n is amino acid position 13.

Preferably, Xaa is Arg.

In another related embodiment, the present invention contemplates a pharmaceutical composition capable of antagonising IL-5, said composition comprising a modified having an amino acid sequence in its first a-helix: Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO:4] wherein Xaa is selected from Arg, Lys, Gin and Asn.

Preferably, Xaa is Arg.

The pharmaceutical compositions may also contain other pharmaceutically active molecules including other IL-5 variants. The modified IL-5 molecule and other components in a WO 97/45448 PCT/AU97/00322 -13pharmaceutical composition are referred to below as "active agents" or "active compounds".

The pharmaceutical forms suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, mannitol glycine or suitable mixtures thereof The preventions of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thirmerosal and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption.

Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredient into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above.

In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and the freeze-drying technique which yield a powder of the active ingredient plus any additional desired ingredient from previously sterile-filtered solution thereof. Mannitol glycine is a particularly useful formulation especially when the modified IL-5 molecules are given as an intravenous drip.

The present invention also extends to forms suitable for inhaling such as a nasal spray as well as in nebulizer form. Alternatively, sustained release compositions may be formulated as well as a range of implant devices. When suitably modified, the molecules may also be given as a cream, lotion or gel. A suitable carrier for a cream, lotion or gel includes a polyol such as but not limited to glycerol, propylene glycol, liquid polyethylene glycol and WO 97/45448 PCT/AU97/00322 -14the like.

Pharmaceutically acceptable carriers and/or diluents include any and all solvents, dispersion media, and antibacterial and antifungal agents. The use of such media and agents for pharmaceutical active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, use thereof in the therapeutic compositions is contemplated by the present invention. Supplementary active ingredients can also be incorporated into the compositions.

It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the mammalian subjects to be treated; each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the novel dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active material and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active material for the treatment of disease in living subjects having a diseased condition involving or facilitated by aberration of IL-5 molecules or levels of The principal active ingredient is compounded for convenient and effective administration in effective amounts with a suitable pharmaceutically acceptable carrier in dosage unit form as hereinbefore disclosed. A unit dosage form can, for example, contain the principal active compound in amounts ranging from 0.5 gg to about 2000 mg. Expressed in proportions, the active compound is generally present in from about 0.5 Ig to about 2000 mg/ml of carrier.

In the case of compositions containing supplementary active ingredients, the dosages are determined by reference to the usual dose and manner of administration of the said ingredients.

The pharmaceutical composition may also comprise genetic molecules such as a vector WO 97/45448 PCT/AU97/00322 capable of transfecting target cells where the vector carries a nucleic acid molecule capable of modulating modified IL-5 expression or modified IL-5 activity. The vector may, for example, be a viral vector.

Still another aspect of the present invention is directed to antibodies to the modified molecules and their derivatives. Such antibodies may be monoclonal or polyclonal and may be specifically raised to modified IL-5 or derivatives thereof. In the case of the latter, a modified IL-5 or its derivatives may first need to be associated with a carrier molecule. The antibodies and/or recombinant modified IL-5 or its derivatives of the present invention are particularly useful as therapeutic or diagnostic agents.

For example, a modified IL-5 and its derivatives can be used to screen for naturally occurring antibodies to IL-5. These may occur, for example in some autoimmune diseases.

Alternatively, specific antibodies can be used to screen for a modified or mutant Techniques for such assays are well known in the art and include, for example, sandwich assays and ELISA. Knowledge of IL-5 levels may be important for diagnosis of certain cancers or a predisposition to cancers or for monitoring certain therapeutic protocols when modified IL-5 molecules are employed.

Antibodies to modified IL-5 of the present invention may be monoclonal or polyclonal.

Alternatively, fragments of antibodies may be used such as Fab fragments. Furthermore, the present invention extends to recombinant and synthetic antibodies and to antibody hybrids. A "synthetic antibody" is considered herein to include fragments and hybrids of antibodies. The antibodies of this aspect of the present invention are particularly useful for immunotherapy and may also be used as a diagnostic tool for assessing apoptosis or monitoring the program of a therapeutic regimen.

For example, specific antibodies can be used to screen for modified IL-5 proteins. The latter would be important, for example, as a means for screening for levels of modified in a cell extract or other biological fluid or purifying modified IL-5 made by recombinant WO 97/45448P PCT/AU97/00322 16means from culture supernatant fluid. Techniques for the assays contemplated herein are known in the art and include, for example, sandwich assays and ELISA.

It is within the scope of this invention to include any second antibodies (monoclonal, polyclonal or fragments of antibodies or synthetic antibodies) directed to the first mentioned antibodies discussed above. Both the first and second antibodies may be used in detection assays or a first antibody may be used with a commercially available anti-immunoglobulin antibody. An antibody as contemplated herein includes any antibody specific to any region of modified Both polyclonal and monoclonal antibodies are obtainable by immunization with the enzyme or protein and either type is utilizable for immunoassays. The methods of obtaining both types of sera are well known in the art. Polyclonal sera are less preferred but are relatively easily prepared by injection of a suitable laboratory animal with an effective amount of modified IL-5 or antigenic parts thereof, collecting serum from the animal, and isolating specific sera by any of the known immunoadsorbent techniques. Although antibodies produced by this method are utilizable in virtually any type of immunoassay, they are generally less favoured because of the potential heterogeneity of the product.

The use of monoclonal antibodies in an immunoassay is particularly preferred because of the ability to produce them in large quantities and the homogeneity of the product. The preparation of hybridoma cell lines for monoclonal antibody production derived by fusing an immortal cell line and lymphocytes sensitized against the immunogenic preparation can be done by techniques which are well known to those who are skilled in the art.

Another aspect of the present invention contemplates a method for detecting modified in a biological sample from a subject said method comprising contacting said biological sample with an antibody specific for modified IL-5 or its derivatives or homologues for a time and under conditions sufficient for an antibody-modified IL-5 complex to form, and then detecting said complex.

WO 97/45448 PCT/AU97/00322 17- The present invention also contemplates genetic assays such as involving PCR analysis to detect a modified IL-5 gene or its derivatives. Alternative methods include direct nucleotide sequencing or mutation scanning such as single stranded conformation polymorphism analysis (SSCP) as well as specific oligonucleotide hybridisation.

The present invention extends to nucleic acid molecules encoding a modified IL-5 of the present invention. Such nucleic acid molecules may be DNA or RNA. When the nucleic acid molecule is in DNA form, it may be genomic DNA or cDNA. RNA forms of the nucleic acid molecules of the present invention are generally mRNA.

Although the nucleic acid molecules of the present invention are generally in isolated form, they may be integrated into or ligated to or otherwise fused or associated with other genetic molecules such as vector molecules and in particular expression vector molecules. Vectors and expression vectors are generally capable of replication and, if applicable, expression in one or both of a prokaryotic cell or a eukaryotic cell. Preferably, prokaryotic cells include E. coli, Bacillus sp and Pseudomonas sp. Preferred eukaryotic cells include yeast, fungal, mammalian and insect cells.

Accordingly, another aspect of the present invention contemplates a genetic construct comprising a vector portion and a mammalian and more particularly a human modified gene portion, which modified IL-5 gene portion is capable of encoding an modified polypeptide or a functional or immunologically interactive derivative thereof.

Preferably, the modified IL-5 gene portion of the genetic construct is operably linked to a promoter on the vector such that said promoter is capable of directing expression of said modified IL-5 gene portion in an appropriate cell.

In addition, the modified IL-5 gene portion of the genetic construct may comprise all or part of the gene fused to another genetic sequence such as a nucleotide sequence encoding glutathione-S-transferase or part thereof.

WO 97/45448 PCT/AU97/00322 -18- Accordingly, another aspect of the present invention contemplates an isolated nucleic acid molecule comprising a sequence of nucleotides encoding or complementary to a sequence encoding a modified IL-5, said modified IL-5 comprising a sequence of amino acids with a first a-helix wherein one or more exposed amino acids in said first a-helix having acidic or acidic-like properties are substituted with a basic amino acid residue or a non-acidic amino acid residue.

Preferably the sequence of nucleotides encodes a human modified IL-5 or a mammalian homologue which comprises a sequence of amino acids in a first a-helix of: Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO:4] wherein Xaa is a basic or non-acidic amino acid residue preferably selected from Arg, Lys, Gin and Asn and wherein said modified IL-5 exhibits antagonism of IL-5 induced mitogenic effects. Preferably, Xaa is Xaa" where n is amino acid position 13. Preferably, Xaa is Arg.

The present invention extends to such genetic constructs and to prokaryotic or eukaryotic cells comprising same.

The present invention further contemplates the use of a modified IL-5 as hereinbefore defined in the manufacture of a medicament in the treatment of allergy, some myeloid leukemias (such as eosinophilic myeloid leukaemia), idiopathic eosinophilic syndrome, allergic inflammations such as asthma, rhinitis and skin allergies.

The present invention is further described by reference to the following non-limiting Examples and/or Figures.

In the Figures: WO 97/45448 PCT/AU97/00322 -19- Figure 1 is a diagrammatic representation of the IL-5 El 3R bacterial expression plasmid pPP31 and DNA/amino acid sequence of MHYH 3 -IL-5 (E13R). The E13R mutation is circled.

Figure 2 is a graphical representation showing the titration E13R for its ability to antagonise IL-5-mediated proliferation of TF .8 cells.

IL-5 WT IL-5 E13R WT@ 3ng/ml+E13R Figure 3A shows the titration of E13R for its ability to antagonise proliferation of TFl-8 cells. This figure also shows that E13R exhibits no detectable agonist activity.

WT

IL-5 E13R IL-5 +IL-5 E13R Figure 3B shows the failure of E13R to antagonise IL-3-mediated proliferation of TFl.8 cells.

0- IL-5 E13R IL-3 IL-5 E13R Figure 3C shows the failure of E13R to antagonise GM-CSF-mediated proliferation of TF1.8 cells.

WO 97/45448 PCT/AU97/00322 IL-5 E13R GM IL-5 E13R Figure 4 is a graphical representation showing the titration of IL-3 for its mitogenic effects on TF1.8 cells and the failure of high levels of El3R to interfere with this activity.

IL-3 E13R@100ng/ml+IL3 Figure 5 is a graphical representation showing TF 1.8 proliferation assay for IL-5 WT IL-5 E13R WTIL5@3ng/ml+E13R WT IL5 (HI) WT IL-5 (HI) was prepared in inclusion bodies in bacteria, dimerized in 2M urea, purified by reverse phase HPLC, concentrated in buffer exchange in PBS It was never dried down.

WT was prepared in the same way as WT IL-5 (HI) but after concentration, the preparation was dried down and dissolved in 25mM glycine and 1.25 mg/ml of mannitol.

Figure 6 is a graphical representation showing the titration of GM-CSF for its mitogenic effects on TF1.8 cells and the failure of high levels of E13R to interfere with this activity.

WT

GM

SE13R@1000ng/ml+GM WO 97/45448 PCT/AU97/00322 -21 Figure 7 is a graphical representation showing that E13R inhibits IL-5 induced eosinophil antibody-dependent cell-mediated cytotoxicity.

E13R+IL-5 E13R+GM-CSF In the absence of GM-CSF and IL-5, the 5 "Cr-release was 4%.

Figure 8 is a graphical representation showing that E13R inhibits IL-5 induced eosinophil colony formation.

E13R+IL-5 E13R+GM-CSF No eosinophil colonies were detected absent of GM-CSF and WO 97/45448 PCT/AU97/00322 -22- EXAMPLE 1 Production of a charge-reversal mutant of interleukin 5(IL-5) with properties The inventors expressed and purified a charge-reversal mutant of IL-5 in which the glutamate residue at position 13 (E13) is replaced by an arginine residue [E13R]. This mutant, E13R, shows specific antagonism of IL-5 mitogenic effects in vitro. In order to maximise expression, this protein is presently being expressed as a fusion protein with the leader sequence MHYH 3 (MHYHHH) [SEQ ID NO:3]. The leader sequence comprises amino acids 1 to 6 in SEQ ID NOs: 1 and 2. However, there is no reason to believe that removal of this leader sequence (or its replacement by another leader sequence) will affect the antagonistic effects of E13R. A diagrammatic representation of MHYH 3 and the sequence of E13R is shown in Figure 1. The nucleotide and corresponding amino acid sequence of E13R is represented in SEQ ID NOs: 1 and 2.

EXAMPLE 2 Production of a bacterial expression plasmid encoding MHYH 3 IL-5 (E13R) Human IL-5 E13R mutant coding sequence was derived from IL-5 wild-type sequence, by site-specific mutagenesis.

Codon 13 was changed to an Arg codon CAG CGT The additional codon changes listed here were based on bacterial codon preferences to promote translational efficiency in E. coli, and did not lead to amino acid changes.

codon 82: CAA CAG codon 83: AAA AAG codon 84: AAA AAG codon 90: AGA CGT codon 91: CGG CGT codon 92: AGA CGT WO 97/45448 PCT/AU97/00322 -23codon 97: CTA CTG codon 110: GAG GAA codon 112: ATA ATC codon 113 ATA ATC codon 115 AGT TCC.

The mutant DNA fragment was modified by polymerase chain reaction using primers to generate a sequence encoding the leader MHYH 3 at the 5' end, and a stop codon at the 3' end. The amplified fragment was subcloned into the plasmid pEC611 to generate the expression plasmid pPP31, containing the tre promoter.

EXAMPLE 3 Production, isolation and refolding of MHYH 3 IL-5 (E13R) Fermentation is by the Fed-Batch method. Escherichia coli MM294/pACYCLacl transformed with the E13R-expressing plasmid pPP31 is grown in minimal medium at 37°C. The pH is held constant at pH 7.0 during bacterial growth by the addition of NH4OH. Expression of IL-5 (E13R) is induced at high cell density (OD6o 20) either by addition of IPTG or by feeding with lactose. Induction continues for 5 hours (not including lag phase) with the protein being expressed as insoluble inclusion bodies (IBs).

Cells are lysed by high pressure homogenization. A primary separation of IBs from cellular debris is done by differential centrifugation, after which the IBs are washed and homogenized one more time. A final centrifugation step isolates IBs of sufficient cleanliness for refolding.

The conditions for fermentation, induction, isolation and refolding are as follows: 1. FERMENTATION A. Inoculation 1. Escherichia coli MM294/pACYClacI 1 carrying pPP31 was streaked out from a 80°C glycerol stock onto a minimal medium plate containing ampicillin (100j/g/ml) and kanamycin (30[/g/ml) and grown overnight at 37 0

C.

WO 97/45448 PCT/AU97/00322 -24- 2. Multiple colonies were assessed for IL-5 (E13R) expression levels by microscopic examination of 20ml shake flask cultures containing inducer, or by colony size on agar plates with or without inducer, in this instance with IPTG.

3. The selected single colony from an agar plate was transferred into 20ml of nitrogen-rich minimal medium containing amplicillin (100 pg/ml) kanamycin The culture was grown at 37"C overnight with agitation.

4. An amount of 10L of C2 was sterilised in a 22 L fermenter and inoculated to a very low density with overnight culture. Growth was at 37"C and a pH of 7.0 was maintained by the addition of NH 4 OH or H 2

SO

4 Agitation was manually controlled and aeration automatically controlled with oxygen saturation levels remaining above 10% v/v p 0 2. Following depletion of glucose at 16-20 hours the cell mass was fed with a concentrated glucose solution containing additional salts. Nutrient feed flow rate was determined by pH or oxygen saturated levels.

B. Induction 1. At an optical density of A6oo 20 the recombinant expression of E13R antagonist was induced, in this instance by changing to a lactose based nutrient feed.

Induction continued for 5 hours at 37"C, pH 7.0. Samples were removed at immediately preceding induction and each hour post-induction and examined by microscopy for the presence of IB, their size being determined by disc centrifugation.

2. The culture was stored overnight at 4"C.

2. PRIMARY ISOLATION OF IL-5 (E13R) INCLUSION BODIES 1. Homogenization Step 1 The culture was passed five times through a Gaulin 30CD homogenizer at 13,500 psi, with homogenate being cooled between passes. Homogenate was then diluted with an WO 97/45448 PCT/AU97/00322 equal volume of RO H 2

O.

The IB size was redetermined by disc centrifugation.

2. Centrifugation Step 1 The homogenate was centrifuged in a Westfalia SB-7 separator with a constant speed of 9,210 rpm at a flow rate determined by IB size.

The concentrate collected from this first step was diluted to w/v.

3. Homogenization Step 2 The IB suspension was passed once through a Gaulin 30CD homogenizer at 13,500 psi.

The IB size was again determined by disc centrifugation.

4. Centrifugation Step 2 The homogenate was centrifuged in a Westfalia SA-1 separator with a constant speed of 9,700 rpm at a flow rate determined by IB size.

For refolding, IBs are initially dissolved in 6M Guanidine-HCl, containing 5-10mM dithiothretiol and buffered to pH 9.5. Reduced IL-5 monomers of MHYH 3 IL-5(E13R) are then purified from non-reduced protein by Size Exclusion Chromatography (eg. Superose 12 [Pharmacia]) in 6M Guanidine-HCl, containing 5mM dithiothreitol and buffered to pH The purified reduced monomer is then refolded into dimers by dilution into 2M urea buffered to pH 9.5 at a final protein concentration of 0.01-0.1 mg/ml. Purification of correctly folded dimers is by reversed phase chromatography on a High Performance Liquid Chromatograph (HPLC) using a suitable column (eg. Brownlee butyl-silica employing 0.1 v/v trifluoroacetic acid (TFA) in water as Buffer A and 0.1 v/v TFA in acetonitrile as Buffer Purified, correctly folded protein can be recovered in biological buffers after lyophilization from HPLC buffers in the presence of mannitol and glycine, WO 97/45448 PCT/AU97/00322 -26added as bulking excipient agents. The identity of the purified protein can be confirmed using mass spectrometry and N-terminal sequencing.

EXAMPLE 4 Assay for Il-5-antagonistic activity The biological assay for IL-5 antagonism by E13R uses incorporation of radiolabelled thymidine to detect IL-5-induced cellular proliferation. The cell line used, TF1.8, is a subline of TF1, a human erythroleukemic cell line which expresses the receptors for IL-3 and GM-CSF. TF1.8 differs from TF1 in that it has been selected for expression of the IL-5 receptor. Stimulation of TF1.8 proliferation by IL-5 is inhibited by 50% in the presence of a 30-fold excess of E13R, and abolished in the presence of a 300-fold excess (Figure However, stimulation of TF1.8 proliferation by IL3 or GM-CSF is unaffected by the presence of E13R. These results demonstrate that E13R is a specific and potent antagonist of IL-5. The 3 H-thymidine assay was conducted as follows: Wash cells 24hrs, prior to assay (1500rpm/5min/RT) and resuspend them in cytokine free medium.

Dilute standards and samples to concentrations required, in culture medium mentioned above (range between 1000ng/ml-lpg/ml as example). Dilutions were done in triplicate.

Aliquot dilutions of appropriate standards (GM-CSF, IL-3, IL-5, etc.) and samples in 100ul final volumes, in sterile 96 well, flat bottom microtitre plates.

Check viability of washed cells and resuspend cells to a concentration of 5 x 104 cells/well.

Each cell contains 100ul of diluted standard/sample 100ul resuspended cells.

WO 97/45448 PCT/AU97/00322 -27- Plates are incubated 37°C for 72 hrs, in a humidified CO 2 incubator (48 hrs, for TF-1 TF1.8 cells).

Pulse cells with 3 H-Thymidine (1/20 dilution of stock, then add Incubate plates 37'C for 5 hrs in a humidified CO 2 incubator.

Harvest contents of each well onto filterpaper using cell harvester and determine the radioactivity incorporated into the DNA, by liquid scintillation counting.

The results are shown in Figures 2 to 6. The results show that E13R specifically antagonises IL-5 activity but does not interfere with GM-CSF or IL-3 activity. In particular, the Figures show that E13R does not antagonise TF1.8 cell proliferation induced by IL-3 nor does it antagonise TF1.8 cell proliferation induced by GM-CSF.

WO 97/45448 PCT/AU97/00322 -28- EXAMPLE Lys" Thr Ser Ala Leu Val Lys Lys Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO. EXAMPLE 6 Arg 13 Thr Ser Ala Leu Val Lys Arg Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO. 6] EXAMPLE 7 Gin 1 3 Thr Ser Ala Leu Val Lys Gin Thr Leu Ala Leu La Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO. 7] EXAMPLE 8 Asn 1 3 Thr Ser Ala La Val Lys Asn Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ ID NO. 8] EXAMPLE 9 Antagonistic properties of E13R on eosinophils An antibody dependent cell-mediated cytotoxicity (ADCC) assay was used to determine the effect of E13R on eosinophil function. This assay measures the ability of eosinophils to kill target cells radiolabelled with chromium and expressing a certain antibody recognized by the eosinophils (Vadas el al. J Immunol 130: 795, 1983). Blood was collected from a healthy donor and eosinophils were prepared by metrizamide gradient separation of leukocytes after removal of red cells with dextran (Vadas et al. J Immunol 122: 1228, 1979).

The eosinophil purity exceeded 95%. The eosinophils were then incubated with the target cells at a ratio of 100 eosinophils per target cell, and a fixed dose of either GM-CSF WO 97/45448 PCT/AU97/00322 -29ng/ml) or IL-5 (10 ng/ml) and a titration of E13R. Figure 7 shows that E13R dosedependently antagonised IL-5-induced ADCC, but had no effect on GM-CSF-induced ADCC. Values are mean and sem of triplicates.

EXAMPLE Effect of E13R on proliferation and differentiation of eosinophilic progenitors Bone marrow was collected from a healthy donor and subjected to density centrifugation (Lymphoprep.). The mononuclear cells were plated in agar supplemented with a fixed dose of either GM-CSF (10 ng/ml) or IL-5 (10 ng/ml) and a titration of E13R. The cells were allowed to grow at 37'C for 2 weeks. The agar plates were then fixed in gluteraldehyde before the plates were stained with Luxol Fast Blue to detect eosinophilic colonies. Figure 8 shows that E13R dose-dependently antagonized the colony-promoting effects of IL-5, while E13R had no effect on GM-CSF stimulation of eosinophilic colony formation. Values are mean and sem of triplicates.

Those skilled in the art will appreciate that the invention described herein is susceptible to variations and modifications other than those specifically described. It is to be understood that the invention includes all such variations and modifications. The invention also includes all of the steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any two or more of said steps or features.

WO 97/45448 PCT/AU97/00322 SEQUENCE LISTING GENERAL INFORMATION: APPLICANT (Other than US) BRESAGEN LIMITED and MEDVET SCIENCE PTY LTD (ii) TITLE OF INVENTION: "AN

ANTAGONIST"

(iii) NUMBER OF SEQUENCES: 8 (iv) CORRESPONDENCE ADDRESS: ADDRESSEE: Davies Collison Cave STREET: 1 Little Collins Street CITY: Melbourne STATE: Victoria COUNTRY: Australia ZIP: 3000 COMPUTER READABLE FORM: MEDIUM TYPE: floppy disc COMPUTER: IBM PC compatible OPERATING SYSTEM: PC-DOS/MS-DOS SOFTWARE: PatentIn Release Version #1.25 (vii) CURRENT APPLICATION DATA: APPLICATION NUMBER: INTERNATIONAL PCT FILING DATE: (vii) PRIOR APPLICATION DATA: APPLICATION NUMBER: PO0054 FILING DATE: 24-MAY-1996 (viii) ATTORNEY/AGENT INFORMATION: NAME: HUGHES, DR E JOHN L REFERENCE/DOCKET NUMBER: EJH/EK (ix) TELECOMMUNICATION INFORMATION: TELEPHONE: +61 3 9254 2777 TELEFAX: +61 3 9254 2770 WO 97/45448 PCT/AU97/00322 -31 INFORMATION FOR SEQ ID NO:1: SEQUENCE CHARACTERISTICS: LENGTH: 377 base pairs TYPE: nucleic acid STRANDEDNESS: single TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: NAME/KEY: CDS LOCATION: 4..366 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: CAT ATG Met 1 CAC TAT CAC CAT CAC ATC CCC ACA His Tyr His His His Ile Pro Thr 5 ATT CCC ACA AGT GCA Ile Pro Thr Ser Ala TTG GTG AAA CGT Leu Val Lys Arg TTG GCA CTG CTT Leu Ala Leu Leu ACT CAT CGA ACT Thr His Arg Thr CTG CTG Leu Leu ATA GCC AAT Ile Ala Asn ACT CTG AGG ATT CCT GTT CCT GTA CAT Thr Leu Arg Ile Pro Val Pro Val His AAA AAT CAC Lys Asn His CTG GAG AGT Leu Glu Ser CAA CTG TGC ACT GAA GAA ATC Gin Leu Cys Thr Glu Glu Ile CAG GGA ATA GGC Gin Gly Ile Gly CAA ACT Gin Thr GTG CAA GGG GGT Val Gin Gly Gly GTG GAA AGA CTA Val Glu Arg Leu AAA AAC TTG TCC Lys Asn Leu Ser

TTA

Leu ATA AAG AAA TAC Ile Lys Lys Tyr GAC GGC CAG AAG Asp Gly Gin Lys AAG TGT GGA GAA Lye Cys Gly Glu CGT CGT CGT GTA AAC CAA TTC CTG GAC Arg Arg Arg Val Asn Gin Phe Leu Asp 100 CTG CAA GAG TTT CTT GGT Leu Gln Glu Phe Leu Gly 110 GTA ATG AAC Val Met Asn GAA TGG ATC ATC Glu Trp Ile Ile TCC TGATGAAGCT T Ser WO 97/45448 PCT/AU97/00322 -32- INFORMATION FOR SEQ ID NO:2: SEQUENCE CHARACTERISTICS: LENGTH: 121 amino acids TYPE: amino acid TOPOLOGY: linear (ii) MOLECULE TYPE: protein Met 1 Val Ala Leu Thr Ile Arg Met (2) (xi) SEQUENCE His Tyr His His 5 Lys Arg Thr Leu Asn Glu Thr Leu Cys Thr Glu Glu Val Gin Gly Gly Lys Lys Tyr Ile Arg Val Asn Gin 100 Asn Thr Glu Trp 115 INFORMATION FOR DESCRIPTION: SEQ ID His Ile Pro Thr Glu 10 Ala Leu Leu Ser Thr Arg Ile Pro Val Pro 40 Ile Phe Gin Gly Ile 55 Thr Val Glu Arg Leu 70 Asp Gly Gin Lys Lys 90 Phe Leu Asp Tyr Leu 105 Ile Ile Glu Ser 120 SEQ ID NO:3: NO:2: Ile Pro His Arg Val His Gly Thr Phe Lys Lys Cys Gin Glu Thr Thr Lys Leu Asn Gly Phe Ala Leu His Ser Ser Glu Gly SEQUENCE CHARACTERISTICS: LENGTH: 6 amino acids TYPE: amino acid TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: Met His Tyr His His His INFORMATION FOR SEQ ID NO. 4: P:\OPER\MRO\27584-97 SPEDOC 23/6/00 -33

SEQUENCE

(A)

(B)

(C)

(D)

CHARACTERISTICS:

LENGTH: 21 TYPE: amino acid STRANDEDNESS: single TOPOLOGY: linear (ii) MOLECULE TYPE: polypeptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO. 4: Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala *pea **19 40 *r 0~ INFORMATION FOR SEQ ID NO. SEQUENCE CHARACTERISTICS: LENGTH: 21 TYPE: amino acid STRANDEDNESS: single TOPOLOGY: linear (ii) MOLECULE TYPE: polypeptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO. a *004 0 p 0S0*

C

go 0 0 Thr Ser Ala Leu Val Lys Lys Thr Leu Ala Leu 10 Leu Ser Thr His Arg Thr Leu Leu Ile Ala INFORMATION FOR SEQ ID NO. 6: SEQUENCE CHARACTERISTICS: LENGTH: 21 TYPE: amino acid STRANDEDNESS: single TOPOLOGY: linear (ii) MOLECULE TYPE: polypeptide P:\OPER\MRO\27584-97 SPE.DOC- 23/6/00 -34- (xi) SEQUENCE DESCRIPTION: SEQ ID NO. 6: Thr Ser Ala Leu Val Lys Arg Thr Leu Ala Leu Leu Ser Thr His Arg 10 Thr Leu Leu Ile Ala INFORMATION FOR SEQ ID NO. 7: SEQUENCE CHARACTERISTICS: LENGTH: 21 TYPE: amino acid STRANDEDNESS: single TOPOLOGY: linear (ii) MOLECULE TYPE: polypeptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO. 7: Thr Ser Ala Leu Val Lys Gin Thr Leu Ala Leu Leu Ser Thr His Arg 0* 5 10 Thr Leu Leu Ile Ala e o(2) INFORMATION FOR SEQ ID NO. 8: SEQUENCE CHARACTERISTICS: s** LENGTH: 21 TYPE: amino acid STRANDEDNESS: single se *o TOPOLOGY: linear

S

(ii) MOLECULE TYPE: polypeptide S* 0 (xi) SEQUENCE DESCRIPTION: SEQ ID NO. 8: Thr Ser Ala Leu Val Lys Asn Thr Leu Ala Leu Leu Ser Thr His Arg 10 Thr Leu Leu Ile Ala

Claims (29)

1. A modified interleukin-5 (IL-5) comprising a sequence of amino acids within a first a- helix wherein one or more exposed amino acids in said a-helix having acidic or acidic-like properties are substituted with a basic amino acid residue or a non-acidic amino acid residue and wherein said modified IL-5 acts as an antagonist of IL-5, subject to the proviso that: wherein said one or more exposed amino acids in said a-helix having acidic or acidic-like properties are substituted with a basic amino acid residue, said modified is characterized by being in a glycosylated form; and (ii) wherein said one or more exposed amino acids in said a-helix having acidic or acidic-like properties are substituted with a non-acidic amino acid residue, said modified is other than IL-5 Gln 1 3 (E13Q).

2. A modified IL-5 according to claim 1, wherein the IL-5 subject to modification is of mammalian origin.

3. A modified IL-5 according to claim 2, wherein the IL-5 subject to modification is of human origin. O.

4. A modified IL-5 according to any one of claims 1 to 3, characterized by being in glycosylated form, and wherein the modification is a substitution of Glu at amino acid position 13 or equivalent position for Arg or Lys or a chemical equivalent or derivative thereof.

A modified IL-5 according to any one of claims 1 to 3, wherein the modification is a substitution of Glu at amino acid position 13 or equivalent position for Asn or a chemical equivalent or derivative thereof.

6. A modified human IL-5 or a mammalian homologue thereof comprising a sequence of amino acids in a first a-helix of: Thr Ser Ala Leu Val Lys Xaa Thr Leu Ala Leu Leu Ser Thr His Arg Thr Leu Leu Ile Ala [SEQ JD NO: 4] P:\OPER\MRO\27584-97 SPEDOC 23/6/00 -36- wherein Xaa is a basic or non-acidic amino acid selected from the group consisting of Arg, Lys, and Asn and wherein said modified IL-5 is an antagonist of IL-5 induced mitogenic effects, subject to the proviso that wherein said one or more exposed amino acids in said a-helix having acidic or acidic-like properties are substituted with Arg or Lys, said modified IL-5 is characterized by being in a glycosylated form.

7. A modified human IL-5 or a mammalian homologue thereof according to claim 6, wherein Xaa is Arg.

8. A modified human IL-5 or a mammalian homologue thereof according to claim 6, wherein Xaa is Lys.

9. A modified human IL-5 or a mammalian homologue thereof according to claim 6, wherein Xaa is Asn.

10. An IL-5 antagonist comprising an IL-5 molecule with a modification to the amino acid sequence in its first a-helix wherein amino acid residue 13 is substituted by a basic amino acid residue or a non-acidic amino acid residue, subject to the proviso that: wherein said amino acid residue 13 is substituted with a basic amino acid residue, said IL-5 antagonist is characterized by being in a glycosylated form; and (ii) wherein said amino acid residue 13 is substituted with a non-acidic amino acid residue, said IL-5 antagonist is other than IL-5 Gln 13 (E13Q). s*

11. An IL-5 antagonist according to claim 10, wherein the basic amino acid residue or non- 00 acidic amino acid residue 13 is selected from the group consisting of Arg, Lys and Asn.

12. An IL-5 antagonist according to claim 10 or 11 comprising an amino acid sequence substantially as set forth in SEQ ID NO: 2.

13. A pharmaceutical composition comprising the modified IL-5 or mammalian homologue Sthereof according to any one of claims 1 to 9 or the IL-5 antagonist according to any one of P:\OPER\MRO\27584-97 SPEDOC 23/6/00 -37- claims 10 to 12 in combination with one or more pharmaceutically acceptable carriers and/or diluents.

14. Use of the modified IL-5 or mammalian homologue thereof according to any one of claims 1 to 9 in the preparation of a medicament for the treatment of an aberrant effect of a native or mutant IL-5 in a mammal.

A use of the modified IL-5 or mammalian homologue thereof according to claim 14 wherein the aberrant effect is a mitogenic effect of

16. A use of the modified IL-5 or mammalian homologue thereof according to claim 14 wherein the aberrant effect is selected from the group consisting of allergy, myeloid leukemia, idiopathic eosinophilic syndrome, allergic inflammation, asthma, rhinitis and skin allergy.

17. Use of the IL-5 antagonist according to any one of claims 10 to 12 in the preparation S. of a medicament for the treatment of an aberrant effect of a native or mutant IL-5 in a mammal. *0

18. A use of the IL-5 antagonist according to claim 17 wherein the aberrant effect is a mitogenic effect of

19. A use of the IL-5 antagonist according to claim 17 wherein the aberrant effect is selected from the group consisting of allergy, myeloid leukemia, idiopathic eosinophilic syndrome, allergic inflammation, asthma, rhinitis and skin allergy.

A method of ameliorating the aberrant effect of a native or mutant IL-5 in a mammal comprising administering to said mammal an effective amount of the modified IL-5 or homologue thereof according to any one of claims 1 to 9 for a time and under conditions sufficient for effecting said treatment.

21. A method of ameliorating the aberrant effect of a native or mutant IL-5 in a mammal P L ccording to claim 20 wherein the aberrant effect is a mitogenic effect of P:\OPER\MRO\27584-97 SPEDOC- 23/6/00 -38-

22. A method of ameliorating the aberrant effect of a native or mutant IL-5 in a mammal according to claim 20 wherein the aberrant effect is selected from the group consisting of allergy, myeloid leukemia, idiopathic eosinophilic syndrome, allergic inflammation, asthma, rhinitis and skin allergy.

23. A method of producing a modified IL-5 or homologue thereof according to any one of claims 1 to 9, said method comprising introducing into a cell a genetic construct capable of expressing said modified IL-5 or homologue thereof, subjecting the cell to conditions to permit the expression of said modified IL-5 or homologue thereof, collecting the expressed modified or homologue thereof in the form of inclusion bodies and subjecting said inclusion bodies to dissolution in a chaotropic agent under conditions of high pH sufficient to disperse and partially unfold said modified IL-5 or homologue thereof, diluting and subjecting said modified or homologue thereof to refolding conditions and then purifying said refolded modified IL- 5 or homologue thereof.

24. A method of producing a modified IL-5 or homologue thereof according to claim 23 wherein the chaotropic agent is urea.

A method of producing a modified IL-5 or homologue thereof according to claim 23 or 24 wherein the modified IL-5 or homologue thereof is expressed as a fusion protein with a o leader sequence.

26. A method of producing a modified IL-5 or homologue thereof according to claim wherein the leader sequence consists of the amino acid sequence Met His Tyr His His His.

27. A modified IL-5 or homologue thereof according to any one of claims 1 to 9 substantially as hereinbefore described with reference to the accompanying examples and drawings. R

28. An IL-5 antagonist according to any one of claims 10 to 12 substantially as hereinbefore P:\OPER\MRO\27584-97 SPEDOC 23/6/00 -39- described with reference to the accompanying examples and drawings.

29. A method of producing a modified IL-5 or homologue thereof according to any one of claims 23 to 26 substantially as hereinbefore described with reference to the accompanying examples and drawings. DATED this TWENTY THIRD day of JUNE, 2000 Medvet Science Pty Ltd AND Bresagen Limited by DAVIES COLLISON CAVE Patent Attorneys for the applicant(s) 00..0 o So *O* *0 o* S o* s Ce