AU713568B2 - Pharmaceutical composition of hedehog proteins and use thereof - Google Patents

Abstract

A pharmaceutical composition of a hedgehog protein which is characterized in that the hedgehog protein is bound to a hydrophilic carrier that is biocompatible and biodegradable wherein the carrier is a polymer which - binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, - does not denature the hedgehog protein when it binds to the carrier, - contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, - contains the charge in the form of acidic groups, - has an average molecular weight of at least 50,000 Da - and contains no agarose reversibly and actively releases hedgehog proteins in vivo from a carrier in a delayed manner.

Description

S F Ref: 447428

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT

ORIGINAL

0

S

Name and Address of Applicant: Actual Inventor(s): Address for Service: Invention Title: Boehringer Mannhelm GmbH 116 Sandhofer Strasse D-68305 Mannheim

GERMANY

Kurt Lang and Apollon Papadimltrlou Spruson Ferguson, Patent Attorneys Level 33 St Martins Tower, 31 Market Street Sydney, New South Wales, 2000, Australia Pharmaceutical composition of Hedgehog Proteins and Use Thereof The following statement is a full description of this invention, including the best method of performing it known to me/us:- 5845 Ref. 20'190 The invention concerns a pharmaceutical composition of hedgehog proteins and its use in particular for the local release of these proteins on bones and cartilage.

Hedgehog (hh) proteins are understood as a family of secreted signal proteins which are responsible for the formation of numerous structures in embryogenesis Smith, Cell 76 (1994) 193 196, N. Perrimon, Cell 80 (1995) 517 520, C. Chiang et al., Nature 83 (1996) 407, M.J. Bitgood et al., Curr. Biol. 6 (1996) 296, A. Vortkamp et al., Science 273 (1996) 613, C.J. Lai et al., Development 121 (1995) 2349). During its biosynthesis a 20 kD Nterminal domain and a 25 kD C-terminal domain are obtained after cleavage of the signal sequence and autocatalytic cleavage. The N-terminal domain is modified in its natural form with cholesterol Porter et al., Science 274 (1996) 255 259). In higher life-forms the hh family is composed of at least three members i.e. sonic, indian and desert hh (Shh, Ihh, Dhh; M. Fietz et al., Development (Suppl.) (1994) 43 51). Differences in the activity of hedgehog proteins that were produced recombinantly were observed after production in .:prokaryotes and eukaryotes Hynes et al., Neuron 15 (1995) 35 44 and T. Nakamura et al., Biochem. Biophys. Res. Comm. 237 (1997) 465 469.

Hynes et al. compare the activity of hh in the supernatant of transformed human embryonic kidney 293 cells (eukaryotic hh) with hh produced from E. coli and find a fourfold higher activity of hh from the supernatants of the kidney cell line. A potential additional accessory factor which is only expressed in eukaryotic cells, a post-translational 20 modification, a different N-terminus since the hh isolated from E. coli contains 50 of a hh which carries two additional N-terminal amino acids (Gly-Ser) or is shortened by 5 6 amino acids, or a higher state of aggregation by binding to nickel agarose beads) have been discussed to be the reason for this increased activity.

Nakamura et al. compare the activity of shh in the supernatant of transformed chicken embryo fibroblasts with an shh fusion protein isolated from E. coli which still has an Nterminal polyhistidine part. The shh in the supernatant of the fibroblasts has a seven-fold higher activity than the purified E. coli protein with regard to stimulation of alkaline phosphatase (AP) in C3H10T /2 cells. Molecules such as bone morphogenetic proteins (BMPs) have been discussed as the reason for the increased activity which are only present in the supernatant of eukaryotic cells and cause the stronger induction of AP.

Kinto et al., FEBS Letters, 404 (1997) 319 323 describe that fibroblasts which secrete hh induce ectopic bone formation in an i.m. implantation on collagen. Hedgehog proteins therefore have an osteoinductive activity.

SR/5.1.99 -2- A process for the production of delivery systems for proteins with delayed release using alginate is known from WO 90/08551. In this process a two-phase system is formed in which the first phase contains a high concentration of the protein (saturated solution) and the second phase contains alginate. However, such a phase separation is difficult and complicated to carry out when producing pharmaceutical compositions in large amounts.

A pulsatile release of dextran from calcium-alginate complexes is known from Kikuchi, A.

et al., J. Controlled Release 47 (1997) 21 29. However, the coupling of hedgehog proteins to such complexes is not described by Kikuchi.

Robinson, C.J. et al. describe in Trends in Biotechnology 14 (1996) 451 452 the intraventricular implantation of alginate microspheres as a method for the local application of NGF or NGF-secreting cells. However, an application for hedgehog proteins is not described.

Downs, E.C. et al. describe in J. Cell. Physiol. 152 (1992) 422 429 the application of calcium alginate spheres as a delivery system for angiogenesis factors. However, the use of 15 this process or this delivery system for hedgehog proteins is not described.

Crey, C.J. and J. Dowsett describe in Biotechnol. Bioeng. 31 (1988) 607 612 the use of calcium/zinc alginate spheres as a delivery system for insulin. However, the use of this process for the production of delivery systems for hedgehog proteins is not known from this.

From Yang et al., Development 124 (1997) 4393- 4404 it is known that high local hedgehog concentrations must be maintained over a period of at least 16 h at the site of action in the 2 body for a pharmaceutically effective in vivo activity. The carrier system described by Yang et al. like the hedgehog-loaded chromatography medium Affigel CM, the Ni-agarose described by Marti et al. in Nature 375 (1995) 322 325 or the Affigel Blue used by Lopez- Martinez et al. in Curr. Biol. 5 (1995) 791 796 or the heparin-agarose particles that were used therein are unsuitable for a pharmaceutical application since they are immunogenic and can cause inflammatory reactions.

The inventors found that the biocompatible and biodegradable carrier collagen described by Kinto et al. for hh-expressing cells is also unsuitable for an optimal local pharmaceutical application of hedgehog proteins as long as these hedgehog proteins bind to the carrier only via ionic interactions. It was found that when collagen carriers are loaded with hedgehog proteins under physiological conditions (pH ca. 7 and in weak acids (up to pH 3 the majority of the applied hedgehog protein is released within minutes from the matrix. According to the inventors' findings, this insufficient binding is due to the lack of sufficient ionic interactions between the hedgehog protein and the carrier. In the case of loading under acidic conditions (below pH a large amount of the applied hedgehog Sprotein is denatured and irreversibly bound to the carrier.

Hence the object of the invention is to provide a pharmaceutical composition of a hedgehog protein with a biocompatible carrier (also referred to hereafter as "carrier matrix") wherein the carrier binds the hedgehog protein in its active, folded structure and can release it in vivo in its active form in a delayed manner. Such formulations are especially suitable i, 0 for repairing bond and cartilage defects, but can also be used to repair neuronal defects or for a systematic delivery.

o• The object is achieved by a first aspect of the present invention which provides a pharmaceutical composition of a hedgehog protein which is characterized in that the hedgehog protein is bound to a hydrophilic carrier which is biocompatible wherein the is carrier is a polymer which 0 0 binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, the carrier contains at least 0.1 to 1, preferably 0.1 to 2 negatively-charged residues per monomer under neutral conditions, 00 the charge is mediated in the form of acidic groups such as sulfate, carboxyl or S phosphate groups, Come se. and the average molecular weight of the carrier is at least 50,000 Da.

A second aspect of the present invention provides a process for the production of a 2 pharmaceutical composition which contains a hedgehog protein which is bound to a 0:9 hydrophilic carrier that is biocompatible, wherein the carrier is a polymer which binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, s0 contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose;

/A

[:\DAYLIB\LIBA]02820.doc:TLT 3a wherein a hedgehog protein in a pharmaceutically effective amount is used and wherein said process includes the step of incubating said hedgehog protein with said hydrophilic carrier in a buffered aqueous solution.

A third aspect of the present invention provides an insoluble carrier containing at least 3 mrg hedgehog protein and at least 10 mmol/ arginine or argininium ions per ml carrier; wherein said carrier is a polymer which binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, Ji contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, a 0 contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose.

I A fourth aspect of the present invention provides a process for the production of an o•C o* insoluble carrier matrix which contains a hedgehog protein, wherein said carrier is a o polymer or an inorganic insoluble phosphate which binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, N *2 does not denature the hedgehog protein when it binds to the carrier, contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose; wherein the carrier matrix is incubated with a solution containing a hedgehog 0 6 protein at a concentration of 3 mg/ml or more and arginine or argininium ions at a concentration of 10 mmol/ or more and the carrier matrix coated in this manner is isolated.

It has surprisingly turned out that the hedgehog proteins can be released reversibly ;io From a carrier in vivo in an active form and in a delayed manner without causing homogeneous and/or inflammatory reactions in vivo if they are bound to a negativelycharged soluble or insoluble polymer matrix.

Preferably a hydrophilic carrier matrix is used and particularly preferably a soluble or insoluble organic hydrophilic carrier matrix. The carrier matrix is particularly preferably composed of an anionic poly-saccharide such as preferably hyaluronic acid (as well as [I :\DAYLIB\LIBAIO282o.doc:,TLT 3b chemically cross-linked forms thereof), chondroitin sulfate, polyvinyl sulfate, keratin sulfate, dextran sulfate, pectin, carrageenans and other hydrocolloids, sulfated alginate, dermatan sulfate, alginate, preferably calcium alginate or a combination of at least two such anionic polysaccharides or a combination of these charged polysaccharides with other polymers, such as in particular collagen, in which the percentage by weight of the charged 0 0 *0* e 4 9 *rr [I:\DAYLIB\[AIBA]02820.doc:TLT -4polysaccharides is 10-50 Insoluble matrix in the sense of the invention means that the matrix is essentially not decomposed or does not visibly dissolve in buffered solution in vitro within 10 20 hours at room temperature. In this connection it is particularly preferable that the carrier used according to the invention contains less than 50 preferably less than 20 and particularly preferably essentially no amount of a neutral polysaccharide. Hyaluronic acid with a molecular weight of at least 106 Dalton, in particular with a molecular weight of 4 x 106 Dalton is particularly suitable as a carrier matrix.

In a further embodiment it has turned out that hydrophilic carriers based on inorganic insoluble phosphate such as hydroxylapatite or tricalcium phosphate are also suitable according to the invention as an insoluble carrier matrix.

A delayed release according to the invention is understood as a release of the hedgehog .o protein in a pharmacologically effective dose over a defined period of at least 14 hours. A pharmacological effect is understood as a neurological effect on nerve cells, a chondrogenesis and/or chondrogenesis induction and preferably osteogenesis and/or osteoinduction as described by Kinto et al., FEBS Letters, 404 (1997) 319-323 for bone induction, by Miao et al. in J. Neurosci. 17 (1997) 5891-5899 for the effect on nerve cells and by Stott et al. in J. Cell. Sci. 110 (1997) 2691-2701 for cartilage cell induction.

An enzymatically degradable carrier is preferably used as the carrier which can be degraded :20 by secreted enzymes proteases) from the cells on which the local in vivo application is carried out. However, the half-life of the carrier should be at least 12 h, but can be several weeks. If the carrier is composed of a polysaccharide, this carrier is preferably degraded by glycosidases and by hydrolases that are present in the cell and secreted. Such a biodegradability of the carrier is, however, not necessary in every case. If the release is carried out to treat osteoporosis or neuronal diseases, a biodegradability is not necessary.

However, such carriers are preferably poorly soluble under physiological conditions and consequently are absorbed by the body over a relative long period (several weeks to months).

Solutions of the hedgehog proteins in high concentrations are necessary in order to produce carrier matrices which are coated with hedgehog proteins in such a manner that they exhibit an adequate pharmaceutical efficacy when applied locally. It has turned out that carriers coated with hedgehog protein that can be used pharmaceutically should preferably contain a concentration of the hedgehog protein of 1 to 5 mg/ml, preferably 3 mg/ml carrier, or more. Carriers are particularly preferred which contain hedgehog proteins at a concentration of 10 mg/ml or more. Hedgehog proteins are intrinsically poorly soluble. However, it has surprisingly turned out that the solubility of the hedgehog proteins increases drastically in solutions which contain arginine or argininium ions (preferably argininium sulfate). Hence, a further subject matter of the invention are aqueous solutions of hedgehog proteins at a concentration of 3 mg/ml and more which contain arginine and argininium ions and are preferably buffered. A further subject matter of the invention is a process for the production of a carrier matrix coated with hedgehog protein which is characterized in that the carrier matrix is incubated with a hedgehog protein solution at a concentration of 3 mg/ml which contains arginine or argininium ions and the carrier matrix coated in this manner is isolated.

Such solutions are suitable for producing carrier matrices which contain hedgehog proteins in pharmaceutically effective concentrations and are suitable for a pharmaceutical application. Hence a further subject matter of the invention is a carrier matrix which contains 3 mg hedgehog protein or more, preferably 10 mg or more per ml carrier matrix and arginine or argininium ions. The concentration of arginine is preferably between and 500 mmol/1, preferably in a pH range between 6 and 8.

Activity within the sense of the invention is understood as the activity of alkaline phosphatase (stimulation of the expression of alkaline phosphatase) which the polypeptide can induce in mammalian cells (activity in the alkaline phosphatase assay). For this a mouse mesenchymal cell line is cultured in a medium which contains fetal calf serum.

Subsequently sterile-filtered sample is added, the cells are lysed after ca. 5 days and alkaline phosphatase is determined in the cell lysate by means of the cleavage of a chromogenic substrate (pNP, p-nitrophenol) Asahina, Exp. Cell. Res. 222 (1996) 38 47 and T.

Nakamura (1997)).

A hedgehog protein is understood by the invention as a secreted signal protein which is responsible for the formation of numerous structures in embryogenesis. Sonic, indian or desert hh are particularly preferably used (Fietz et al. Development (Suppl.) (1994) 43- 51). A hh protein with a sequence as described in the EMBL database under the No. L38518 is preferably used. Proteins of the hedgehog family exhibit a pronounced homology in their amino acid sequence which is why it is also preferable to express those nucleic acids which code for hedgehog proteins which are 80 or more homologous with the above-mentioned sequence of sonic hedgehog protein.

The human sonic hedgehog precursor protein is composed of the amino acids 1 462 of the sequence described in the EMBL database under No. L38518. The amino acids 1 23 -6represent the signal peptide, the amino acids 24 197 represent the mature signal domain, the amino acids 32 197 represent the signal domain shortened by 8 amino acids and the amino acids 198 462 represent the auto-processing domain after autoproteolytic cleavage.

The pharmaceutical composition according to the invention preferably contains an additional polymer which essentially acts as a supporting structural substance, which preferably also has an adhesion function for cells but does not bind on hedgehog proteins based on ionic interactions. Preferably, this substance is a biodegradable protein or a hydrolytic degradation product which may be used, for example, in the form of intact protein fibers as solubilized protein or as partially hydrolyzed protein. Such a supporting structural substance is preferably collagen, gelatin, elastin, or fibrin. The supporting structural substance is preferably present in a lower amount than the described hydrophilic biocompatible carrier according to the invention. The proportion of the supporting structural substance is therefore preferably 30%, or less preferably 10% or less. However, the supporting structural substance can also be present in excess relative to the hydrophilic S 15 carrier. It only needs to be ensured in this connection that the amount of hydrophilic carrier in the pharmaceutical composition according to the invention be sufficiently high to ensure that a therapeutically effective amount of the hedgehog protein is bound to the hydrophilic carrier. It is therefore preferred to use at least a 5-fold excess of the hydrophilic S-carrier referred to the hedgehog protein. In addition, the binding of the hedgehog protein 20 to the carrier for the preparation of the pharmaceutical composition should be effected at a pH of 4.5 or greater. As pointed out above, it has been found that hedgehog proteins at a pH below 4.5, denature and bind irreversibly to proteinous carrier-like collagen. Therefore the binding of the hedgehog protein to the carrier should be effected in the neutral pH range.

'25 Carriers which contain, apart from the hydrophilic carrier, further supporting structural compounds are, for example, protein polysaccharide complexes. Preferred complexes are described in U.S. Patent 4,614,794.

The pharmaceutical composition is prepared by incubating the hedgehog protein with the hydrophilic carrier at a pH of 4.5 or greater, preferably a pH within the neutral range (pH 6 to whereby the binding of the hedgehog protein to the carrier is effected. The incubation is carried out preferably in a buffered solution. When using as the carrier a matrix which additionally contains a biodegradable protein such as collagen, incubation at a pH of 4.5 or greater will ensure the prevention of irreversible binding of hedgehog protein (which is denatured at lower pH values) to the biodegradable protein. It has been found that under these conditions, no binding, or only a negligible binding, of the hedgehog protein to the biodegradable protein occurs as long as the hedgehog protein does not contain a hydrophobic modification, and thus the biodegradable protein merely acts as a supporting structural substance.

Hydrophobically modified (lipophilised) hedgehog proteins are hedgehog proteins which show in relation to unmodified hh proteins recombinantly produced in prokaryotes) an increase in surface hydrophobicity. The degree of lipophilisation of a protein is measured by integration in a lipid layer according to Haque, et al., J. Agric. Food Chem.

(1982) 481. Such lipophilised hh proteins bind according to the invention to the hydrophilic carrier in the same manner as non-lipophilised hh protein does, but they bind, in addition, to the biodegradable protein via hydrophobic interactions.

For the production of the pharmaceutical composition it is additionally preferable to add auxiliary substances such as sugars (mannitol, sucrose, lactose, glucose, sucrose, trehalose, preferably 20-100 mg/ml) or amino acids such as glycine or arginine as well as antioxidants such as EDTA, citrate, polyethylene glycol (1-10 by weight), detergents, preferably non- 15 ionic detergents (preferably 0.005 1 by weight) such as polysorbates (Tween®20 or Tween®80) or polyoxyethylenes, anti-inflammatory ingredients, local anaesthetics, antibiotics and/or stabilizers such as lipids, fatty acids and glycerol.

In a further preferred embodiment, a pharmaceutical composition of the hedgehog protein according to the invention together with suramin is preferred and this can be used S. 20 advantageously.

The pharmaceutical compositions can contain additional pharmaceutical auxiliary substances.

In a preferred embodiment the pharmaceutical composition contains hedgehog protein at a concentration of 0.1 100 mg/ml.

In a preferred embodiment the pharmaceutical composition additionally contains a pharmaceutically acceptable buffer which is biocompatible preferably in a range between pH 4 and pH 10, particularly preferably in a range between pH 6 and 8, in particular at a pH value of ca. pH 7. The pH value of the pharmaceutical composition should be expediently more than pH 4 in order to prevent a denaturation and detachment of the zinc complexed in the hedgehog protein. The concentration of the buffer is preferably 1-500 mmol/1, in particular 5-150 mmol/1 and particularly preferably 10-100 mmol/1. In a -8suitable embodiment 20 mmol/1 potassium phosphate buffer, pH 7.2 or 100 mmol/1 arginine chloride pH 7.2 is used as the buffer.

The following examples, publications and figures further elucidate the invention, the protective scope of which results from the patent claims. The described methods are to be understood as examples which still describe the subject matter of the invention even after modifications.

Description of the Figures: Figure 1: In vitro release of shh from a collagen matrix.

Figure 2: In vitro release of shh from calcium alginate capsules.

10 Figure 3: In vitro release of shh from hyaluronic acid gels.

Figure 4: In vitro release of shh from an alginate collagen matrix.

Examples S* Example 1 Production of an alginate gel containing hh protein An aliquot of hh protein solution (1 mg/ml shh solution in 50 mg/ml sucrose, 50 mM potassium phosphate, pH 7.2) is stirred with an Na-alginate stock solution (Pronova, Biopolymer, NO) (in water, greater than 0.1 in such a way that a gelatinous alginate protein mixture is formed. This gel can either be used directly as an injectable matrix or further processed into calcium alginate capsules or is stored as a lyophilisate.

Example 2 Production of a collagen mixture containing hh protein (comparative example) 100 l of a hh solution (1 mg/ml hh) in a) 20 mM potassium phosphate, pH 7.4 or b) in 50 mM sodium acetate, pH 4.5 or c) in 0.1 trifluoroacetic acid, pH 2 is added dropwise onto collagen sponges (Helistat, Integra Life Sciences, USA) with a size of 10 x 10 x 3 mm. The loaded carriers are then frozen (-70 0 lyophilized and analysed.

For this the loaded sponges are incubated at 37 0 C in a suitable volume in buffer (10 mmol/1 potassium phosphate, 150 mmol/1 NaC1, pH The amount of released hh is determined by means of RP-HPLC.

Example 3 3.1 Production of Ca-alginate capsules The alginate gel described in example 1 which contains hh protein is added dropwise to a CaC12 solution (about 1.5 while stirring continuously. Ca-alginate complexes form spontaneously which contain the protein. The size of the capsules that are formed depends on the size of the drops and can be varied as desired. After a 5 to 10 minute incubation in the CaCl 2 solution, the capsules are filtered and washed in buffer (20 mmol/1 potassium phosphate, pH These capsules can either be used directly as implants or be processed further by lyophilization.

3.2 Lyophilization The Ca-alginate capsules are frozen at -70 0 C and subsequently lyophilized. The 15 lyophilization enables a stable storage of the capsules and additionally facilitates the implantation since the capsules are easier to handle in the dry state.

Example 4 In vitro release of Shh 20 The analysis of the in vitro release kinetics shows that Shh is released in a delayed manner from Ca-alginate capsules over a period of at least 70 h (Fig. 2) whereas shh from a collagen matrix is released within a few, and up to ca. 20, minutes (Fig. Lyophilized alginate spheres containing shh were incubated at 37 0 C in 10 mM potassium phosphate, 150 mM NaC1, pH 7.2 (Rotatherm). After 5 min, lh, 5h, 10h, Id, 34h, 2d, 3d and 6d samples are taken and analysed for their shh content by means of SDS polyacrylamide electrophoresis (Fig. The cumulative amount of shh released from the alginate capsules is plotted against time.

Examination of the release kinetics of shh from 2.5 hyaluronic acid gels using hyaluronic acid types with a low molecular weight (LMW; molecular weight ca. 1.3 x 106 Da) or with a high molecular weight (HMW; molecular weight ca. 4 x 106 Da) is shown in Figure 3. For this hyaluronic acid gels loaded with shh are filled into dialysis tubes (separation size 300,000 Da) and the tubes are incubated in PBS at 37 0 C. At the stated times samples of the release medium were taken and analysed for their shh content by means of reversed phase HPLC. It is clear that a portion of the loaded hedgehog protein is released in a delayed manner. Another portion of the protein remains bound to the hyaluronic acid and could be released in vivo by degradation of the hyaluronic acid.

Examination of the release kinetics of shh from a collagen-alginate matrix (Fibracol T M cf.

U.S. Patent 4,614,794) is shown in Figure 4. For this a Fibracol sponge (1 x 1 x 0.3 cm) was loaded with 0.2 mg of hshh in PBS. The loaded sponges were frozen (-70 0 lyophilized and incubated in an appropriate volume of PBS at 37 0 C. At the stated times samples of the release medium were taken and analyzed for their shh content by means of reversed phase HPLC. It is clear that only about 10 to 20% of the loaded hedgehog proteins are released into the medium and that a major portion remains bound to the collagen-alginate matrix.

This portion could be released in vivo by degradation of the matrix.

List of References 15 Asahina, Exp. Cell. Res. 222 (1996) 38-47 Bitgood, M.J. et al., Curr. Biol. 6 (1996) 296 Chiang, et al., Nature 83 (1996) 407 Crey, C.J. et al., Biotechnol. Bioeng. 31 (1988) 607-612 Downs, E.C. et al., J. Cellular Physiology 152 (1992) 422-429 20 Fietz, M. et al., Development (Suppl) (1994) 43-51 Haque, Z. et al., J. Agric. Food Chem. 30 (1982) 481 Hynes, M. et al., Neuron 15 (1995) 35-44 Karablis et al., Genes and Development 8 (1994) 277-289 Kikuchi, A. et al., J. Controlled Release 47 (1997) 21-29 Kinto et al., FEBS Letters, 404 (1997) 319-323 Lai, C.J. et al., Development 121 (1995) 2349 Lopez-Martinez et al., Curr. Biol. 5 (1995) 791-796 Marti et al., Nature 375 (1995) 322-325 Miao et al., J. Neurosci. 17 (1997) 5891-5899 Nakamura, T. et al., Biochem. Biophys. Res. Comm. 237 (1997) 465-469 Perrimon, Cell 80 (1995) 517-520 Porter, J.A. et al., Science 274 (1996) 255-259 Robinson, C.J. et al., Trends in Biotechnology 14 (1996) 451-452 Smith, Cell 76 (1994) 193-196 Stott et al., J. Cell Sci. 110 (1997) 2691-2701

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11 U.S. Patent 4,614,794 Vortkamp, A. et al., Science 273 (1996) 613 WO 90/0855 1 Yang et al., Development 124 (1997) 4393-4404

Claims (15)

1. Pharmaceutical composition of a hedgehog protein which is characterized in that the hedgehog protein is bound to a hydrophilic carrier which is biocompatible wherein the carrier is a polymer which binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions. 111 contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose.

2. Pharmaceutical composition as claimed in claim 1, wherein the carrier is composed of an anionic polysaccharide. I 3. Pharmaceutical composition as claimed in claims 1 or 2, wherein the *0 hydrophilic carrier is hyaluronic acid.

4. Pharmaceutical composition as claimed in any one of claims 1-3, wherein the pharmaceutical composition contains a hedgehog protein at a concentration of 0.1 100mg/ml. *2 0

5. Pharmaceutical composition as claimed in any one of claims 1-4, wherein the composition is buffered in the range between pH 4 and

6. Pharmaceutical composition as claimed in any one of claims 1-5, wherein the composition contains arginine or argininium ions.

7. A process for the production of a pharmaceutical composition which r contains a hedgehog protein which is bound to a hydrophilic carrier that is biocompatible, wherein the carrier is a polymer which O:mose binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, i o contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose; [I:\I)AYI,IB\L-IBA]02820.doc:TLT 13 wherein a hedgehog protein in a pharmaceutically effective amount is used and wherein said process includes the step of incubating said hedgehog protein with said hydrophilic carrier in a buffered aqueous solution.

8. Process as claimed in claim 7, wherein a hedgehog protein is used at a concentration of 0.1-100 mg/ml.

9. Process for the delayed release of a hedgehog protein in the human body, wherein the hedgehog protein is applied locally in the human body in a pharmaceutical composition as claimed in any one of claims Process as claimed in claim 9, wherein the hedgehog protein is applied at a :o concentration of 0.1 to 100 mg/ml. 1. Process as claimed in any one of claims 7-10, wherein the hydrophilic carrier is hyaluronic acid.

12. Process as claimed in any one of claims 7-11, wherein the binding of the hedgehog protein to the hydrophilic carrier is effected by incubation at a pH of 4.5 or greater. 0

13. An insoluble carrier containing at least 3 mg hedgehog protein and at least mmol/l arginine or argininium ions per ml carrier; wherein said carrier is a polymer which binds the hedgehog protein as a negatively-charged carrier as a result of ionic interactions, does not denature the hedgehog protein when it binds to the carrier, o.oo contains at least 0.1 to 2 negatively-charged residues per monomer under neutral S conditions, 0 contains the charge in the form of acidic groups, has an average molecular weight of at least 50,000 Da and contains no agarose.

14. Process for the production of an insoluble carrier which contains a hedgehog protein, wherein said carrier is a polymer which binds the hedgehog protein as a negatively-charged carrier as a result of ionic 1o interactions, does not denature the hedgehog protein when it binds to the carrier, contains at least 0.1 to 2 negatively-charged residues per monomer under neutral conditions, contains the charge in the form of acidic groups, Ho ,'has an average molecular weight of at least 50,000 Da [1:\DAYLIB\,IBA]02820.doc:T-rT and contains no agarose; wherein the carrier is incubated with a solution containing a hedgehog protein at a concentration of 3 mg/ml or more and arginine or argininium ions at a concentration of mmol/l or more and the carrier coated in this manner is isolated. Process as claimed in claim 14, wherein the binding of the hedgehog protein to the carrier is effected by incubation at a pH of 4.5 or greater.

16. Pharmaceutical composition of a hedgehog protein, substantially as hereinbcfore described with reference to any one of the Examples.

17. Process for the production of a pharmaceutical composition which contains a hedgehog protein, substantially as hereinbefore described with reference to any one of the Examples.

18. Insoluble carrier matrix containing at least 3 mg hedgehog protein, substantially as hereinbefore described with reference to any one of the Examples.

19. Process for the production of an insoluble carrier, substantially as hereinbefore described with reference to any one of the Examples. Dated 14 October, 1999 Boehringer Mannheim GmbH AW se* .00.. 0 0 0 *00: S S S 0@S C OSO S 0O e S o *o 0 o* O eS S c C oooe2( oooo O o oo Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON rI :\DAYL1\LIBA]02820.doc:TLT