AU705932B2 - Sertoli cells as neurorecovery inducing cells for neurodegenerative disorders - Google Patents

Description

WO 96/28030 PCT/US96/03335 -1- SERTOLI CELLS AS NEURORECOVERY INDUCING

CELLS

FOR NEURODEGENERATIVE

DISORDERS

TECHNICAL FIELD The present invention generally relates to cell transplantation and specifically to a method of transplanting cells which, following transplantation into the central nervous system (CNS), ameliorates the behavioral and functional deficits associated with neurological and neurodegenerative disorders.

BACKGROUND OF THE INVENTION In treating disease it is often useful to treat tissue locally, rather than systemically, with trophic factors, particularly areas of tissue damage as for example in wound healing.

As a further example, transplantation of neural tissue into the mammalian central nervous system (CNS) is becoming an alternative treatment for neurological and neurodegenerative disorders including epilepsy, stroke, Huntington's diseases, head injury, spinal injury, pain, Parkinson's disease, myelin deficiencies, neuromuscular disorders, neurological pain, amyotrophic lateral sclerosis, Alzheimer's disease, and affective disorders of the brain. Preclinical and clinical WO 96/28030 PCT/US9603335 -2data indicate that transplanted cells (the graft) used in cell transplantation protocols for these types of neurodegenerative diseases survive and integrate with the host tissue, and provides functional recovery. (Sanberg et al., 1994).

The primary source for these grafts has been the fetus. For example, fetal ventral mesencephalic tissue has been demonstrated to be a viable graft source in Parkinson's disease.

(Lindvall et al., 1990; Bjorklund, 1992).

Likewise, fetal striatal tissue has been utilized successfully as graft material in Huntington's disease. (Isacson et al., 1986; Sanberg et al., 1994).

Neurologically dysfunctional animals have been transplanted with non-fetal cells and nonneuronal cells/tissue. For example, chromaffin cells from adult donors have been used in the treatment of Parkinson's disease. The major advantage of this type of transplantation protocol is that the graft source is not a fetal source and, thereby, circumvents the ethical and logistical problems associated with acquiring fetal tissue.

Utilizing the chromaffin cell protocol, normalization of behavior is observed. However, the functional recovery of this behavior is WO 96/28030 PCT/US96/03335 -3temporary and the animals revert to their pretransplantation status (Bjorklund and Stenevi, 1985; Lindvall et al., 1987). The inability of this type of treatment protocol to maintain normal behavioral activity in animals in the Parkinson's disease model renders clinical application of this protocol as well as other treatment therapies premature.

Administration of growth factors as a means of treating neurological and neurodegenerative diseases has been contemplated in the art. However, delivering these agents to the brain is fraught with great difficulties that have yet to be successfully overcome. Generally, these agents cannot be administered systemically and infusion into the brain is an impractical and imperfect solution. Engineering cells to deliver specific, single trophic factors when implanted in the brain has been suggested, but stable transfection and survival of the cells when implanted in the brain continues to be problematic.

Additionally, it is becoming increasingly recognized that multiple trophic factors acting in concert are likely to be necessary for the successful treatment of neurological and neurodegenerative conditions.

WO 96/28030 PCT/US96/03335 -4- Long term maintenance of functional recovery has been observed in a diabetic animal model utilizing a novel transplantation treatment protocol utilizing isolated islet cells and Sertoli cells. It is clear that the efficacy of the treatment is due to the presence of the Sertoli cells, in part, due to their known immunosuppressive secretory factor. (Selawry and Cameron, 1993; Cameron et al., 1990). Sertoli cells are also known to secrete a number of important trophic growth factors.

Accordingly, it would be desirable to utilize Sertoli cells alone as a source for diseases where growth and trophic factor support of damaged tissue is useful. Examples include, wound healing and neurological disorders including neurodegenerative disorders. The Sertoli cells can be used to function as an in situ factory for trophic factors to thereby hasten wound healing and to ameliorate functional and behavioral deficits associated with neurological and neurodegenerative disorders.

WO 96/28030 PCT/US96/03335 SUMMARY OF THE INVENTION In accordance with the present invention, there is provided a method of generating in situ trophic factor production by transplanting Sertoli cells into a mammal, the cells secreting trophic factors in situ.

BRIEF DESCRIPTION OF THE DRAWINGS Other advantages of the present invention will be readily appreciated as the same becomes better understood by reference to the following detailed description when considered in connection with the accompanying drawings wherein: Figure 1 is a graph showing the results of apomorphine-induced rotational behavior, animals from both groups exhibited >7 rotations per minute or, at least, a total of 210 rotations for minutes (contralateral to the lesion) when challenged with apomorphine pre-transplant, at post-transplant periods, animals receiving media alone continued to display significant rotations, in contrast, animals receiving the Sertoli cells had a marked reductions (more than 60%) in their rotational behavior across the post-transplant periods; WO 96/28030 PCT/US96/03335 -6- Figure 2 is a graph showing biased swing behavior, animals from both groups displayed biased swing activity (contralateral to the lesion) as revealed by the elevated body swing test, at post-transplant periods, animals receiving the media alone continued to display significant biased swing activity, in contrast, animals receiving the Sertoli cells did not exhibit any biased swing behavior across the post-transplant periods; Figure 3A-C are light micrographs illustrating cells from the ventral mesencephalon of fetal rats (VM) isolated and cultured for seven days in control medium (CM) or Sertoli cell preconditioned medium (SCM) and photographed with darkfield, interference contrast optics, wherein depicts VM cells incubated in CM showing no evidence of stimulation or differentiation, depicts VM cells incubated in SCM appearing highly stimulated, and at higher magnification, depicts VM cells incubated in SCM exhibiting neurite outgrowth as a result of Sertoli secreted trophic factors; WO 96/28030 PCT/US96/03335 -7- Figure 4A-B are electron micrographs illustrating the striatum of the brain showing the penetration tract (arrows) and the site of Sertoli cell transplantation, and shows the boxed area in at higher magnification, with higher resolution, Sertoli cells (arrows) are easily identified because of the 14 latex bead inclusions which were loaded into the cells prior to transplantation; and Figure 5A-B are two light micrographs illustrating grafted Sertoli cells in situ labeled with a florescent tag (DiI) prior to their transplantation into the striatum of the brain wherein depicts viable, florescent Sertoli cells in a rat host that had not received immunosuppression therapy with cyclosporine

A

(CsA), and shows viable, florescent Sertoli cells in the rat host that had received cyclosporine A immunosuppression therapy.

DETAILED DESCRIPTION OF THE INVENTION Generally, the present invention provides a method for promoting the repair, protection, and support of dysfunctional tissue by mechanisms including in situ production of Sertoli cellderived growth and regulatory factors referred to WO 96/28030 PCT/US96/03335 -8generally as trophic factors. Additionally, the present method provides a method of generating in situ trophic factor production. This is achieved by transplanting isolated Sertoli cells into a mammal, the cells secreting trophic factors in situ.

One significant benefit of utilizing Sertoli cells as an in situ factory for producing trophic factors is that Sertoli cells have been shown to have an effective immunosuppressant effect. Accordingly, concomitant adjunctive therapy to produce immunosuppression is not required. In other words, the Sertoli cells can be used as a trophic factor source while also providing a self-induced local immunosuppressive effect.

Trophic factors secreted by Sertoli cells include Sertoli cell-derived growth and regulatory factors such as insulin-like growth factors I and II, epidermal growth factor, transforming growth factors a and 3, and interleukin la (Griswold, 1992). For a more extensive list of Sertoli cell secretory factors refer to Table 1. Such factors have been shown to have an ameliorative effect on behavioral and functional deficits associated with neurodegenerative diseases. These factors are well WO 96/28030 PCT1US9603335 -9known tropic factors which support normal cell and tissue metabolism and function. (Griswold, 1992) The present invention utilized the phenomenon that Sertoli cells can produce a trophic-rich, growthsupportive fluid microenvironment at the site of cellular dysfunction or cellular/tissue damage.

Cellular/tissue damage can include, but is not limited to, radiation damage, burns and wounds. In contrast to the Sertoli cell/islet cell transplantation protocol used in the diabetic model, the method of the present invention utilizes only one type of cell, i.e. Sertoli cells, thereby significantly reducing the logistic and procedural problems inherent in attempting to transplant two different cell types at one host site.

Although rat Sertoli cells are utilized in the following examples, Sertoli cells from any suitable source can be used. For example, human Sertoli cells may be used for transplantation in humans. Additionally, in a preferred embodiment of the present invention, porcine Sertoli cells may be transplanted into a mammal, such as a human.

Furthermore, veterinary uses of the present invention are contemplated and allogenic Sertoli cells would be selected for transplantation into the desired mammalian host.

WO 96/28030 PCTUS96/03335 As demonstrated in the experimental section below, the present invention can be utilized as a treatment for ameliorating the behavioral and functional deficits associated with neurodegenerative diseases, such as Huntington's disease and Parkinson's disease. This can be accomplished without the concomitant side effects of previously utilized immunosuppressive adjuvant therapy, such as the chronic use of cyclosporine

A.

The Sertoli cells, to provide both the secretion of the trophic factors and the immunosuppressive effect.

As shown in the examples below, the transplantation of Sertoli cells prior to inducing or formation of a brain lesion can provide a neuroprotective effect. For example, as demonstrated below, implantation of Sertoli cells prior to inducement of a Huntington's type disease provided both neuroprotective and prophylactic effects on a subsequent brain lesion. Therefore, the implantation of Sertoli cells early on following diagnosis of a neurodegenerative disease may provide useful treatment, prevention or reduction of the disease. Additionally, Sertoli cells may be transplanted in other types of CNS trauma such as head injury to treat, prevent, WO 96/28030 PCT/US96/03335 -11and/or prophylactically reduce the effects of CNS injury.

The following example demonstrates the ability of the present invention to ameliorate behavioral deficits associated with neurodegenerative disorders.

EXAMPLE 1: SERTOLI CELL

TRANSPLANTATION

Specific Protocol: The protocol generally involves two basic steps, Sertoli cell isolation and cell transplantation both of which are briefly described below (for greater details regarding the cell isolation see Selawry and Cameron (1993) and for details regarding cell transplantation, see Pakzaban et al.(1993) both incorporated by reference.

1 A) Sertoli Cell Isolation The isolation procedure follows a well defined method Selawry and Cameron, (1993) and is routinely utilized. The cell culture medium used in all isolation steps and in which the cells were incubated was DMEM:Hams F12 supplemented with retinol, ITS, and gentamicin sulfate (Cameron and Muffly, 1991). Testes were surgically collected from sixteen day old male Sprague-Dawley rats. The WO 96/28030 PCT/US96/03335 -12testes were decapsulated and prepared for enzymatic digestion to separate other testicular cell types from the Sertoli cells. The enzymatic procedure utilized collagenase hyaluronidase and trypsin which is a typical procedure used in many cell isolation protocols. After sequential enzymatic digestion, the Sertoli cell isolate was washed with culture medium, transferred to sterile culture vessels and placed in a humidified, 5% CO 2 95% air tissue culture incubator. Following forty-eight hours of preincubation in a 39 0 C incubator, the Sertoli cells were washed to remove any contaminating debris.

The resultant Sertoli cell-enriched fraction was resuspended into 0.25ml of DMEM/F12 medium and incubated at 37 0 C for at least 24 hours.

The Sertoli cells are then liberated from the vessel floor with trypsin, transferred to a sterile conical test tube, and repeatedly washed by centrifugation and treated with trypsin inhibitor to cease the enzymatic action of the trypsin.

During the day of transplantation, the Sertoli cell-enriched fraction is resuspended and suctioned using a Hamilton syringe with a 20 gauge spinal needle.

WO 96/28030 PCT/US96/03335 -13- (1B) Isolation and Pretreatment of Sertoli Cells Alternatively, as previously described (Cameron et al. 1987a; Cameron et al. 1987b) decapsulated rat testes were subjected to sequential enzymatic treatment at 37°C using 0.25% trypsin (Sigma) and 0.1% collagenase (Sigma, type V) (Cameron et al. 1987a; Cameron et al. 1987b).

The resulting Sertoli cell aggregates were equally distributed in a volume of 20ml incubation medium into 75cm 2 tissue culture flasks (Costar). Plated Sertoli aggregates were incubated at 39C in C0 2 -95% air for 48 hours after which cells were subjected to hypotonic treatment with sterile Tris-Hcl buffer for one minute (Galdieri et al.

1981) to expedite the removal of contaminating germ cells. Following two washes with incubation medium, flasks were replenished with incubation medium and returned to the CO2-injected incubator at 370C in 5% C0 2 -95% air. The resulting pre-treated Sertoli-enriched monocultures contained greater than 95% Sertoli cells. Plating density 2.0 X 106 Sertoli cells/cm 2 did not result in a confluent monolayer of cells.

WO 96/28030 PCT/US96/03335 -14- Cell Transplantation The transplantation protocol follows the procedure as previously described (Pakzaban et al., 1993). Animal surgery was carried out under sterile conditions. All animals were initially anesthetized with 0.60 ml/kg sodium pentobarbital and then were placed in a Koph stereotaxic instrument. Unilateral striatal transplants were performed using coordinates set at: anteroposterior mediolateral 2.8, dorsoventral 5.9, and 5.8 (based on the atlas of Paxinos and Watson, 1984). The striatum ipsilateral to the lesioned substantia nigra was transplanted with Sertoli cells. Each striatum receives a total volume of 3 pl of Sertoli cell suspension. One microliter of the Sertoli cell suspension was infused over one minute per dorsoventral site.

Controls only received media. Another five minutes was allowed upon reaching the last dorsoventral site before retracting the needle. After surgery, the animals were placed on heating pads to recover.

Animals receive a short course of immunosuppression using Cyclosporine-A (20 mg/kg/d, immediately after surgery and on the day following transplant.

WO 96/28030 PCTIUS96/03335 However, subsequent studies demonstrated that this short course of Cyclosporine-A is not needed (Figure Sertoli cells are transplanted into animal models of various neurodegenerative disorders by stereotaxic coordinates defined for the specific disorder, as illustrated in the Parkinson's disease example, and then are systemically assayed for functional recovery by techniques specific to that animal model.

The present study used Sprague-Dawley male, eight week old rats with 6-OHDA-induced hemiparkinsonism At three weeks postlesion, the animals were subjected to behavioral tests that included the apomorphine-induced rotational behavior and the swing behavior.

Baseline data showed significant apomorphineinduced rotational behavior (contralateral to the lesioned side of the CNS) in all these animals (at least 200 turns for 30 minutes). Using the elevated body swing test (EBST), significant rightbiased swing activity (more than 70%) was also noted.

WO 96/28030 PCT/US96/03335 -16- At three weeks post-lesion, one group of animals received Sertoli cells and one group was subjected to the same surgical procedure but only received media (DMEM without serum) as controls. All animals received cyclosporine for the first two days following the transplant. At one, one and a half, and two months post-transplant, animals were again introduced in the same behavioral tests.

The animals receiving Sertoli cells exhibited significant reductions in rotations (mean of 50 turns for 30 minutes) while the animals receiving the media alone were at pre-transplant rotational level (Figure The normalization of turning behavior persisted across the two month test period. The right-biased swing activity previously displayed by the Sertoli cells transplanted animals was also significantly reduced at post-transplant test sessions (Figure The animals receiving the media did not show any significant reductions in their right-biased swing responses.

WO 96/28030 PCT/US96/03335 -17- At autopsy, brains were removed from the animals and fixed for vibratome sectioning at Following staining, there was a marked reduction of activated glial cells at the penetration site lesion site) in Sertoli cell transplanted rats when compared to the penetration site in the lesioned animals not transplanted with Sertoli cells.

EXAMPLE 2: GROWTH OF NEURAL

CELLS

Incubation medium and Sertoli cell preconditioned medium The incubation medium used for Sertoli cell culture and co-culture was Dulbecco's Minimum Essential Medium:Hams F12 Nutrient Medium (Whittaker Bioproducts) mixed 1:1 and supplemented with 3mg/ml L-glutamine (Sigma, grade

III),

0.Olcc/ml insulin-transferrin-selenium

(ITS,

Collaborative Research, Inc.), 50 ng/ml retinol (Sigma), 19ji/ml lactic acid (Sigma) and O.Olcc/ml gentamicin sulfate (Gibco).

Following the first 48 hour incubation period of isolated Sertoli cells, media was collected and centrifuged at 1500rpm for 5 minutes.

The supernatent was collected and immediately WO 96/28030 PCTIUS96/03335 -18frozen in sterile test tubes. This medium was identified as Sertoli pre-conditioned medium

(SCM).

Isolation and incubation of fetal brain cells Fetal brain cells (FBC) were collected from the ventral mesencephalon of fetal rats (15-17 days gestation). The fetal brain tissue was suspended in medium and initially dispersed by passing it through a series of sequentially decreasing sized hypodermic needles (18-26 gauge).

The resulting suspension was treated with 0.1% trypsin for five minutes and followed by 0.1% trypsin inhibitor for two minutes. The suspended FBC were washed resuspended in incubation medium and plated in poly-L-lysine-coated culture vessels.

Cells from the ventral mesencephalon of fetal rats (VM) were isolated and cultured for seven days in control medium (CM) or Sertoli cell pre-conditioned medium (SCM) as shown in Figure 3A.

VM cells incubated in CM showed no evidence of cellular stimulation or differentiation. Referring to Figure 3B, VM cells incubated in SCM were highly stimulated. Figure 3C illustrates that at higher magnification, VM cells incubated in SCM show WO 96/28030 PCT/US96/03335 -19neurite outgrowth as a response to Sertoli cell secreted trophic factors.

EXAMPLE 3: IDENTIFICATION OF SERTOLI

CELLS

Incorporation of latex beads: Sertoli cells were isolated and prepared for incubation as described. Prior to transplantation (approximately 12 hours), sterile l1m latex beads (10/ml medium; Pelco, Tustin,

CA)

were added to the incubation medium. Sertoli cells rapidly phagocytosed the beads. Immediately prior to transplantation, the beaded Sertoli cells were washed (three times) and resuspended in iml of incubation medium.

Referring to Figure 4A, Sertoli cells were transplanted into the striatum of the brain wherein the penetration tract (arrows) and the site of Sertoli cell transplantation are shown. At higher magnification as shown in Figure 4B, Sertoli cells (arrows) were easily identified because of the inclusion of 14 latex beads which were loaded into the Sertoli cells prior to transplantation.

PCT/US 6 /03 33 IPEAUS 28 MAR 1997 EXAMPLE 4: EFFECTS OF CYCLOSPORINE A (CSA) ON THE SURVIVAL OF TRANSPLANTED SERTOLI CELLS Fluorescent cell labeling: Immediately prior to transplantation (approximately two hours), Sertoli cell monocultures were treated with CM-DiI fluorescent dye for cell tracking (100gl stock/ml medium; Molecular Probes, Inc., Eugene, OR) for seven minutes at 37 0 C and then placed at 4 0 C for an additional 15 minutes. Fluorescent "tagged" Sertoli cells were washed (3X) and resuspended in Iml of incubation medium.

The effect of cyclosporine A on the survival of grafted Sertoli cells in situ was examined. Grafted Sertoli cells were labeled with a fluorescent tag (DiI) prior to transplantation into the striatum of the brain. The tissue was collected one month post-transplantation.

Referring to Figure 5A, viable fluorescent Sertoli cells were seen in a rat host that had not received immunosuppression therapy with cyclosporine A.

Referring to Figure 5B, viable fluorescent Sertoli cells are shown in a rat host that had received cyclosporine A immunosuppression therapy. This example demonstrates that cyclosporine A is not AMENDED

SHEET

WO 96/28030 PCT/US96/03335 -21necessary for the survival of Sertoli cells transplanted into the brain.

EXAMPLE 5: PROPHYLACTIC EFFECTS OF SERTOLI

CELLS

The transplantation of Sertoli cells is neuroprotective when implanted prior to inducing brain lesions. This prophylactic effect of Sertoli cells was demonstrated in an animal model for Huntington's Disease This model is produced by the systemic administration of the mitochondrial inhibitor, 3 -nitropropronic acid (3NP). It has been demonstrated by Sanberg and colleagues (Koutouzis et al. 1994; Borlongan et al. 1995) and others that the injection of 3NP causes specific lesions within the striatum which mimic the pathology seen in Huntington's disease.

In the present experiment 8 rats were transplanted with rat Sertoli cells (as described previously) unilaterally into one striatum of normal rats. Therefore, one side of the brain had Sertoli cells and the other side was without. One month later, the animals were injected with 3NP as described elsewhere (Koutouzis et al. 1994; Borlongan et al. 1995) to induce HD. Normal rats when injected with 3NP demonstrate bilateral damage of the striatum of the brain and have behavioral WO 96/28030 PCT/US96/03335 -22deficits which are equal on both sides of the body (Koutouzis et al. 1994; Borlongan et la. 1995).

One month following 3NP administration the animals demonstrated unilateral behavioral deficits. This was seen by the demonstration of apomorphine-induced rotations post-lesion in Sertoli transplanted animals, but not in controls (Number of Rotations; Controls=0.25±.6; Sertoli transplanted=197±31.9, p<.0001). This asymmetric rotational behavior was indicative of a lesion on the side of the brain which was not transplanted with Sertoli cells. Therefore, Sertoli cell implants, vis-a-vis trophic mechanisms, have neuroprotective and prophylactic effects on subsequent brain lesions. This provides evidence that Sertoli transplantation may also be useful in treating neurodegenerative diseases early, before significant damage is present.

These results, taken together, show that the Sertoli cells ameliorate the behavioral and functional deficits of animal models of Parkinson's disease and Huntington's disease. The mechanism involved is most likely the secretion of Sertoli cell-derived growth factors, as demonstrated by the sprouting of neuronal tissue as shown in Example 2, and regulatory factors which promote the repair and WO 96/28030 PCTIUS96/03335 -23the prolonged support of the relevant nervous tissue. Additionally, Sertoli cells may protect and promote nervous tissue repair in the brain by inhibiting glial cell activation at the lesion site. These results also demonstrate the viability in situ of transplanted Sertoli cells.

Throughout this application various publications are referenced by citation or number.

Full citations for the publication are listed below. The disclosure of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains.

The invention has been described in an illustrative manner, and it is to be understood the terminology used is intended to be in the nature of description rather than of limitation.

Obviously, many modifications and variations of the present invention are possible in light of the above teachings. Therefore, it is to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described.

WO 96/28030 PCT/US90;/ni33 -24- TABLE 1 I. Sertoli Cell-Derived Growth and Regulatory Factors (Partial List) Category and Protein Hormones/Growth Factors Mullerian Inhibiting Substance Inhibin Insulin-like Growth Factor (Sommatomedins A and C, IGF) Prodynorphin Interleukin-la Transforming Growth Factor a& Basic Fibroblast Growth Factor LHRH-like Factor Function inhibits Mullerian duct inhibits FSH release growth factor mitogen B growth factors growth factor Leydig cell steroidogenesis (unpurified or incompletely characterized) Sertoli Secreted Growth Factor growth factor Seminiferous Growth Factor Leydig Cell Stimulatory Activity Testins CMB proteins vitamin Binding Proteins Transport and Bioprotection Transferrin Ceruloplasm Saposin SGP-2 (Clusterin) Androgen Binding Protein

SPARC

IGF Binding Proteins Riboflavin Binding Protein Proteases and Protease Inhibitors Plasminogen Activator Cyclic Protein-2 Cystatin.

a 2 -Macroglobulin Type IV Collagenase Metalloproteinases vitamin transport iron transport copper transport binds glycosphingolipids lipid transport? transports T and DHT calcium binding protein? IGF transport riboflavin transport protease protease inhibitor protease inhibitor protease inhibitor protease protease Basement membrane Collagen IV Laminin Proteoglycans WO 96/28030 PCTUS96/03335 REFERENCES

CITED

Bjorklund and Stenevi, "Intracerebral neural grafting: a historical perspective" in Bjorklund,

A

and U. Stenevi, eds. Neural grafting in the mammalian CNS, Amsterdam: Elsevier, 3-11 (1985).

Bjorklund, "Dopaminergic transplants in experimental Parkinsonism: Cellular mechanisms of graft-induced functional recovery" Current Biology, 2:683-689 (1992).

Borlongan et al., "PR: Systemic 3 -nitropropionic acid: Behavior deficits and striatal damage in rats" Brain Research Bulletin, 36:549-556 (1995).

Cameron et al., "Successful islet/abdominal testis transplantation does not require Leydig cells" Transplantation, 50:549-556 (1995).

Cameron and Muffly, "Hormonal regulation of spermatid binding to Sertoli cells in vitro." J Cell Sci., 100:523-533 (1991).

Griswold, "Protein Secretion by Sertoli cells: general considerations" in Russell, L.d. and M.D.

Griswold eds. The Sertoli Cell, Cache River Press Clearwater, FL, 195-200 (1992).

Isacson et al., "Graft-induced behavioral recovery in an animal model of Huntington's disease" Proc. Natl.

Acad. Sci., 83:2728-2732 (1986).

Koutouzis et al., "PR:Systemic 3 -nitropropionic acid: Long term effects on locomotor behavior" Brain Research, 646:242-246 (1994).

Lindvall et al., "Transplantation in Parkinson's disease: two cases of adrenal medullary grafts to the putamen" Ann. Neurol. 22:457-468 (1987).

Lindvall et al., "Grafts of fetal dopamine neurons survive and improve motor function in Parkinson's disease" Science, 247:574-577 (1990).

Pakzaban et al., "Increased proportion of Ache-rich zones and improved morphological integration in host striatum of fetal grafts derived from the lateral but not the medial ganglionic eminence" Exp. Brain Res., 97:13-22 (1993) WO 96/28030 PCT/US96/03335 -26- Sanberg et al., "Cell transplantation for Huntington's disease" R.G. Landes Co., Boca Raton, FL, pp.19-21 (1994).

Selawry and Cameron, "Sertoli cell-enriched fractions in successful islet cell transplantation" Cell Transplan., 2:123-129 (1993).

Wictorin et al., "Reformation of long axon pathways in adult rat CNS by human forebrain neuroblasts" Nature, 347:556-558 (1990).

Claims (23)

1. A method of generating in situ trophic factors by transplanting Sertoli cells into a tissue in need of trophic factors of a mammal, the cells creating trophic factors in situ.

2. A method as set forth in claim 1 wherein the tissue in need of trophic factors is the central nervous system of a mammal.

3. A method as set forth in claim 2 wherein the mammal suffers from a neurological disorder including a neural degeneration disorder, said method further including the step of ameliorating behavioral and functional deficits caused by the disorder by the action of the secreted trophic factors.

4. A method as set forth in claim 1, wherein the Sertoli cells are porcine Sertoli cells. A method as set forth in claim 2, wherein said transplanting step is further defined as protecting the central nervous system from degenerative disorders. WO 96/28030 PCTJUS96/03335 -28-

6. A method as set forth in claim 2 wherein said transplanting step is further defined as repairing damaged central nervous system tissue.

7. A method as set forth in claim 3, wherein the neurological disorder or neural degeneration disorder includes epilepsy, stroke, Huntington's disease, head injury, spinal injury, pain, Parkinson's disease, myelin deficiencies, neuromuscular disorders, neurological pain, amyotrophic lateral sclerosis, Alzheimer's disease, and affective disorders of the brain.

8. A method of generating in situ trophic factor production by transplanting porcine Sertoli cells into the central nervous system of a subject, the cells secreting trophic factors in situ, for treating neurological disorders including epilepsy, stroke, Huntington's disease, head injury, spinal injury, pain, Parkinson's disease, myelin deficiencies, neuromuscular disorders, neurological pain, amyotrophic lateral sclerosis, Alzheimer's disease, and affective disorders of the brain.

9. A method as set forth in claim 8, wherein the subject is human. 29 A method of generating in situ trophic factor production by transplanting Sertoli cells into an area of tissue damage of a subject, the Sertoli cells secreting trophic factors in situ.

11. Use of Sertoli cells to generate in situ trophic factors by tranplanting Sertoli cells into a tissue in need of trophic factors of a mammal, the cells creating trophic factors in situ.

12. A use of Sertoli cells as set forth in claim 11 wherein the tissue in need of trophic factors is the central nervous system of a mammal.

13. A use of Sertoli cells as set forth in claim 11 or claim 12 wherein the .mammal suffers from a neurological disorder including a neural degeneration disorder, said use ameliorating behavioral and functional deficits caused by the disorder by the action of the secreted trophic factors. e* e 4

14. A use of Sertoli cells as set forth in any one of claims 11 to 13, wherein the *4 Sertoli cells are porcine Sertoli cells.

15. A use of Sertoli cells as set forth in claim 12, wherein said transplanting is further defined as protecting the central nervous system from degenerative disorders.

16. A use of Sertoli cells as set forth in claim 12 wherein said transplanting is further defined as repairing damaged central nervous system tissue.

17. A use of Sertoli cells as set forth in claim 13, wherein the neurological disorder or neural degeneration disorder includes epilepsy, stroke, Huntington's disease, head injury, spinal injury, pain, Parkinson's disease, myelin deficiencies, neuromuscular disorders, neurological pain, amyotrophic lateral sclerosis, Alzheimer's disease, and affective disorders of the brain.

18. Sertoli cells for use in generating in situ trophic factor production by transplanting porcine Sertoli cells into the central nervous system of a subject, the cells secreting trophic factors in situ, for treating neurological disorders including epilepsy, stroke, Huntington's disease, head injury, spinal injury, pain, Parkinson's disease, myelin deficiencies, neuromuscular disorders, neurological pain, amyotrophic lateral sclerosis, Alzheimer's disease, and affective disorders of the brain.

19. Sertoli cells for use in generating in situ trophic factor production as set forth in claim 18, wherein the subject is human. Sertoli cells for use in generating in situ trophic factor production by transplanting Sertoli cells into an area of tissue damage of a subject, the Sertoli cells secreting trophic factors in situ.

21. A method of generating in situ trophic factors substantially as hereinbefore described with reference to the accompanying Examples 1 to 31

22. A use of Sertoli cells substantially as hereinbefore described with reference to the accomanying Examples 1 to

23. Sertoli cells for use in generating in situ trophic factors substantially as hereinbefore described with reference to the accompanying Examples 1 to

24. A method of generating in situ trophic factors substantially as hereinbefore described with reference to the accompanying Figures 1 to

25. A use of Sertoli cells substantially as hereinbefore described with reference to the accompanying Figures 1 to

26. Sertoli cells for use in generating in situ trophic factors substantially as hereinbefore described with reference to the accompanying Figures 1 to DATED THIS 1 ST DAY OF APRIL 1999. UNIVERSITY OF SOUTH FLORIDA By their Patent Attorneys LORD COMPANY PERTH, WESTERN AUSTRALIA.