AU704292B2

AU704292B2 - Solubilisation methods

Info

Publication number: AU704292B2
Application number: AU73178/96A
Authority: AU
Inventors: Roger Randal Charles New
Original assignee: Cortecs Ltd
Current assignee: Cortecs Ltd
Priority date: 1995-10-25
Filing date: 1996-10-25
Publication date: 1999-04-15
Anticipated expiration: 2016-10-25
Also published as: BR9611343A; EP0857061A1; NO981864D0; ZA969016B; CN1202818A; GB9521805D0; JP2000502990A; KR19990067028A; CA2235487A1; AU7317896A; NZ320442A; NO981864L; WO1997015289A1

Description

WO 97/15289 PCT/GB96/02609 1 SOLUBILISATION

METHODS

The present invention relates to methods of solubilising an agent, eg biologically active materials, in an amphiphile. In particular the invention relates to methods of bringing biologically active substances used for topical administration into association with permeation aids.

It is a continuing objective of the pharmaceutical industry to achieve high degrees of solubilisation of biologically active materials in a variety of solvents.

There are several reasons for this need to achieve solubilisation. For instance, achieving solubilisation in particular solvents may improve bioavailibility. An example of this would be the solubilisation of biologically active materials in oils. Examples of methods to achieve this can be found for example, in WO 95/13795, WO 96/17593 and WO 96/17594.

The methods disclosed in the above-noted patent publications and applications include steps whereby the biologically active material is brought into association with an amphiphile. There are also circumstances where it would be desirable to achieve higher degrees of solubilisation of biologically active molecules, particularly water-soluble ones, in amphiphiles. Examples include: i) to improve dissolution characteristics in aqueous media, eg. to aid in achieving rapid dissolution; ii) to aid incorporation into low HLB systems such as oil mixtures; and 2 iii) to bring about association between biologically active substances and permeation aids, which are often amphiphiles, for topical use.

Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

Thus, in a first aspect, the present invention provides a method of solubilising an agent in an amphiphile which method includes the steps of: 00 bringing the agent and amphiphile into association with each other in a common solvent; (ii) removing the common solvent; and (iii) heating the residue from step (ii); S" "20 In step the agent and the amphiphihile can suitably be brought into association with each other by firstly dissolving each one separately in the common solvent, followed by mixing of the two resultant solutions.

The removal of the solvent should be carried out at a temperature such that the amphiphile/agent residue which remains is in the solid state. The heating step should then be sufficient to melt the solid amphiphile, and also to convert the amphiphile/agent array from an "open" form to one which is more condensed.

The common solvent can be water, for example, and it can be removed in step (ii) by, e.g. freeze drying, centrifugal vacuum drying or any other suitable method.

Suitably, in the above methods the amphiphile will be a phospholipid, for instance lecithin, a glycolipid, a WO 97/15289 PCT/GB96/02609 3 polyoxyethylene containing surfactant, a lipophilic sulphate, betaine, a sarcosine containing surfactant, Solulan 16, Solulan C24, polyoxyethylene 40 stearate, one of the Tween series of surfactants, one of the Span series of surfactants or a pegolated castor oil derivative, e.g. Cremaphor The "agent" is suitably a hydophilic species which is generally soluble in aqueous solvents but insoluble in hydrophobic solvents. The range of hydrophilic species of use in the present invention is diverse but hydrophilic macromolecules represent an example of a species which may be used.

A wide variety of macromolecules is suitable for use in the present invention. In general, the macromolecular compound will be hydrophilic or will at least have hydrophilic regions since there is usually little difficulty in solubilising a hydrophobic macromolecule in oily solutions. Examples of suitable macromolecules include proteins and glycoproteins, oligo and polynucleic acids, for example DNA and RNA, polysaccharides and supramolecular assemblies of any of these including, in some cases, whole cells or organelles. It may also be convenient to co-solubilise a small molecule such as a vitamin in association with a macromolecule, particularly a polysaccharide such as a cyclodextrin. Small molecules such as vitamin B12 may also be chemically conjugated with macromolecules and may thus be included in the compositions.

Examples of particular proteins which may be successfully solubilised by the method of the present invention include insulin, calcitonin, haemoglobin, cytochrome

C,

WO 97/15289 PCT/GB96/02609 4 horseradish peroxidase, aprotinin, mushroom tyrosinase, erythrcpoietin, somatotropin, growth hormone, growth hormone releasing factor, galanin, urokinase, Factor

IX,

tissue plasminogen activator, superoxide dismutase, catalase, peroxidase, ferritin, interferon, Factor

VIII

and fragments thereof (all of the above proteins can be from any suitable source). Other macromolecules may be used are FITC-labelled dextran and RNA extract from Torulla yeast.

It seems that there is no upper limit of molecular weight for the macromolecular compound since dextran having a molecular weight of about 1,000,000 can easily be solubilised by the process of the present invention.

In addition to macromolecules, the process of the present invention is of use in solubilising smaller organic molecules. Examples of small organic molecules include glucose, carboxyfluorescin and many pharmaceutical agents, for example anti-cancer agents, but, of course, the process could equally be applied to other small organic molecules, for example vitamins or pharmaceutically or biologically active agents. In addition, compounds such as calcium chloride and sodium phosphate can also be solubilised using this process.

Indeed, the present invention would be particularly advantageous for pharmaceutically and biologically active agents since the use of non aqueous solutions may enable the route by which the molecule enters the body to be varied, for example to increase bioavailability.

Another type of species which may be included in the hydrophobic compositions of the invention is an inorganic material such as a small inorganic molecule or a WO 97/15289 PCT/GB96/02609 colloidal substance, for example a colloidal metal. The process of the present invention enables some of the properties of a colloidal metal such as colloidal gold, palladium, platinum or rhodium, to be retained even in hydrophobic solvents in which the particles would, under normal circumstances, aggregate. This could be particularly useful for catalysis of reactions carried out in organic solvents.

The above-described method is particularly suitable for achieving association between an agent which is for topical administration and a permeation aid. An example of the former is Zinc Acetate (ZnAc) Particularly suitable amphiphiles are those which are solid at room temperature, eg Solulan 16 and Solulan C24.

In other aspects the present invention provides: i) a composition comprising an agent solubilised in an amphiphile obtainable by any of the methods described herein, particularly an agent for topical administration solubilised in an aphiphile which is a permeation aid; and ii) the use of a composition of the invention in the preparation of a medicament for topical administration, particularly a composition for use in the treatment of inflammation and/or arthritis wherein the active agent is ZnAc 2 Preferred features of each aspect of the invention are as for each other aspect mutatis mutandis.

WO 97/15289 PCT/GB96/02609 6 The invention will now be described with reference to the following examples, which should not be construed as in any way limiting the invention.

Example

I

A solution of zinc acetate at a concentration of 100mg/ml was prepared by addition of 100mg of ZnAc, to iml of distilled water, and mixing at RT until dissolution was achieved.

A solution of Solulan 16 at a concentration of 100mg/ml was prepared by addition of 500mg of Solulan to 4 .5ml of distilled water and mixing at 600C until dissolution was achieved.

Solutions from steps 1 2 were dispensed into 4ml glass screw-capped vials as follows, and mixed well: ZnAc, (Vol) Solulan 16 (Vol) ZnAc, (wt) Solulan 16 (wt) %Zn (wt:wt) Ratio (wt:wt) 1 A B 0.2ml 0.3ml 1.8ml 1.7ml i 20mg 180mg 10 9:1 30mg 170mg 15 5.7:1

C

0.4ml 1.6ml 160mg 4:1 9:1 5.7:1 The vials and contents were frozen in liquid nitrogen and lyophilised overnight with a condenser temperature of -40 0 C, and a vacuum of 0.1mBar.

WO 97/15289 PCT/GB96/02609 7 The following day, the lyophilates were incubated at +600C on a heating block, to melt the solid cake of Solulan S16.

Because of the fact that the solutions solidified at room temperature, dissolution or otherwise of ZnAc 2 in Solulan 16 was assessed visually by examining the clarity or turbidity of the resulting liquid formulations, rather than by recording optical densities.

Results of visual observations are recorded in the table below: Sample Ratio S16:Zn Optical Appearance (wt:wt) Clarity A 9:1 Clear Solution B 5.7:1 Cloudy Paste C 4:1 Cloudy Paste Exanple 2 Solutions of Solulan 16 and ZnAc 2 were prepared as above, and dispensed into 2ml glass screw-capped vials as follows: SUBSTITUTE SHEET (RULE 26) WO 97/15289 PCT/GB96/02609 A B C D E F G S16 0 20 40 80 120 160 200 ZnAc 2 20 20 20 20 20 20 S16 (mg) 0 2 4 8 12 16 ZnAc 2 (mg) 2 2 2 2 2 2 2 Ratio 0:1 1:1 2:1 4:1 6:1 8:1 10:1 S16:Zn After lyophilisation and heating to 60 0 C, the solubility of ZnAc 2 in S16 was assessed visually as described in Example 1. The results of observations are given in the table below: Sample A B C D E F G Ratio (S16:Zn) 0 1 2 4 6 8 Observations after heating Samples remained as white solids Samples turned to a viscous glassy fluid essentially clear free-flowing fluid Example 3 A solution of Solulan C24 at a concentration of 100mg/ml was prepared by addition of 500mg of Solulan C24 to 4.5ml of distilled water and mixing at 60 0 C until dissolution was achieved.

500mg CuAc 2 was dissolved in 10ml of distilled water SUBSTITUTE SHEET (RULE 26) WO 97/15289 PCT/GB96/02609 9 to give a concentration of 15041 of CuAc, solution (7.5mg solid) and 9251 of Solulan C24 solution (92.5mg solid) were dispensed into a 4ml glass screw-capped vial. The vial contents were mixed well, frozen in liquid nitrogen and lyophilised overnight.

10mg of CuAc, was dispensed into a 2ml glass vial and 100mg of Solulan C24 was added. The vials was capped and heated to 60 0 C to melt the oil. The contents of the tube were vortexed to disperse the CuAc 2 in the oil, then incubated at 60 0 C for eight hours. After incubation, the contents of the vial consisted of a colourless oil solution on top of solid undissolved crystals of CuAca.

The following day, the contents of the lyophilised tube was converted to a clear strong blue-coloured solution by incubating in a heating block for 2 minutes at 60 0

C.

The vials from steps and were allowed to stand at room temperature to allow any undissolved CuAc, to sediment, before the oil solidified.

20Ag of solid oil was taken off the surface of each sample in step and dissolved in 1801 of distilled water.

The optical densities of the aqueous solutions obtained in step were measured at 650nm, and compared against reference solutions prepared by dilution of CuAc, solution from step 2 in distilled water. The results are reported in the table below, where it is seen WO 97/15289 PCT/GB96/02609 that a higher concentration of CuAc, dissolved in the oil can be measured by following the lyophilisation procedure described above, than by simple mixing of the components.

Concentration in aqueous solution(mg/ml) Measured Theoretical After 6.7 lyophilisation After simple 0.8 10.0 mixing Example 4 A virus suspension (Sabin strains, Types 1, 2, 3) containing 5x10 8 particles/ml (spun to remove contaminating protein) was diluted 50-fold by addition of 200l of the suspension to 9.9ml of distilled water, yielding a concentration of 10 7 particles/ml. The suspension was divided into four equal aliquots of and dispensed into 7ml screw-capped glass vials. 2.5ml of distilled water was added to one aliquot of virus particles and this group was labelled 2.5ml of Solulan C24 (100mg/ml) was added to another aliquot and mixed gently. This group was labelled 200l of each preparation was dispensed into 10 freezedrying vials, and the remainder in 100l aliquots into other tubes as "pre-drying" controls. The controls were stored overnight at +4 0 C. The freeze-drying vials were placed in the centrifugal rotor of the freeze-dryer and lyophilised overnight.

On the following day 100l of culture medium was added to SUBSTITUTE SHEET (RULE 26) WO 97/15289 PCT/GB96/02609 11 each vial in group and mixed gently. The vials in group were sealed and heated to 600C in a hot water bath for 5 seconds to melt the Solulan C24, which resulted in a claer solution. Upon cooling to room temperature this material solidified. 901 of medium was added to the vials of the group to make the total volume up to 1001. 10l of sample was then transferred from each of groups and to fresh lml vials and Iml of medium was added to each and mixed well.

To fresh iml vials was added 4 x 201 of samples from each of the pre-drying groups and Iml of medium was added to each. The contents of each vial were mixed well.

The suspensions prepared as described herein were used to perform 10-fold dilutions in Vero cell cultures,to measure the viability of the polio virus present. The results were expressed as the highest dilution at which cytopathic effects were observed.

Nature of Sample Highest Dilution at which 50% CPE observed Non-dried control water 10-/10-6 Non-dried control Solulan C24 10-/10- Freeze-dried control water 102/10-2 Freeze-dried control Solulan C24 10-/10- 8

Claims

1. A method of solubilising an agent in an amphiphile which method includes the steps of:- bringing the agent and amphiphile into association with each other in a common solvent; (ii) removing the common solvent; and (iii) heating the residue from step (ii);

2. A method as claimed in claim 1 wherein the agent and the amphiphile ate brought into association with each other by firstly dissolving each one separately in the common solvent, followed by mixing of the two resultant solutions.

3. A method as claimed in claim 1 or claim 2 wherein the common solvent is water.

4. A method as claimed in claim 3 wherein the water is removed in step (iii) by freeze drying, centrifugal vacuum drying or any other suitable method. A method as claimed in any one of claims 1 to 4 wherein the amphiphile is lecithin, a glycolipid, a polyoxyethylene containing surfactant, a lipophilic sulphate, betaine, a sarcosine containing surfactant, Solulan 16, Solulan C24, polyoxyethylene 40 stearate, one of the Tween series of surfactants, one of the Span series of surfactants or a pegolated castor oil derivative, e.g. Cremaphor WO 97/15289 PCT/GB96/02609 13

6. A method as claimed in any one of claims 1 to 4 wherein the amphiphile is a permeation aid.

7. A method as claimed in any one of claims 1 to 6 wherein the agent is one administered topically.

8. A method as claimed in claim 7 wherein the agent is Zinc Acetate.

9. A method as claimed in claim 8 wherein the amphiphile is Solulan 16 or Solulan C24. A method as claimed in any one of claims 1 to wherein the agent is a macromolecule, a small organic or inorganic molecule or a colloidal substance.

11. A method as claimed in claim 10, wherein the macromolecule comprises a protein, glycoprotein, oligo- or polynucleic acid, polysaccharide or supramolecular assembly thereof.

12. A method as claimed in claim 11, wherein the protein is insulin, calcitonin, haemoglobin, cytochrome C, horseradish peroxidase, aprotinin, mushroom tyrosinase, erythropoietin, somatotropin, growth hormone, growth hormone releasing factor, galanin, urokinase, Factor IX, tissue plasminogen activator, superoxide dismutase, catalase, peroxidase, ferritin, interferon, Factor VIII or fragments thereof.

13. A method as claimed in any one of claims 10 to 12 wherein the agent is for oral administration.

14. A method as claimed in claim 13 wherein the agent is a virus. A composition comprising an agent solubilised in an amphiphile obtainable by a method as defined in any one of claims 1 to 14.

16. A composition as defined in claim 15 when used in the preparation of a medicament for topical administration.

17. A composition as claimed in claim 16 wherein the medicament is for the treatment or prevention of inflammation and/or arthritis. A composition as defined in claim 15 when used in the preparation of a medicament for manipulating the immune response. 15

19. A composition as claimed in claim 18 wherein the medicament is a vaccine, S.

20. A method of solubilising an agent in an amphiphile substantially as hereinabefore described with reference to the accompanying examples.

21. A composition substantially as hereinbefore described with reference to the accompanying examples. 0 DATED THIS 14 day of May 1998 CORTECS UK LIMITED Patent Attorneys for the Applicant:- F B RICE CO