AU647449B2 - Method for the immunological determination of ligands - Google Patents

Method for the immunological determination of ligands

Info

Publication number
AU647449B2
AU647449B2 AU71013/91A AU7101391A AU647449B2 AU 647449 B2 AU647449 B2 AU 647449B2 AU 71013/91 A AU71013/91 A AU 71013/91A AU 7101391 A AU7101391 A AU 7101391A AU 647449 B2 AU647449 B2 AU 647449B2
Authority
AU
Australia
Prior art keywords
binding
ligand
determined
immunological determination
ligands
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU71013/91A
Other versions
AU7101391A (en
Inventor
Gerhard Bienhaus
Arno Deger
Roland Schenck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU7101391A publication Critical patent/AU7101391A/en
Application granted granted Critical
Publication of AU647449B2 publication Critical patent/AU647449B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method for the immunological determination of a ligand, in which the ligand to be determined is reacted with a) at least 2 molecules of a substance P2 which is capable of specific binding, with at least one of these molecules carrying a label, and b) a receptor R which consists of a binding partner P1 which is capable of monovalent binding with P2, and of a binding site R1 for the ligand to be determined, and then the label is determined, and a reagent for the immunological determination of a ligand, which contains a substance P2 which is capable of specific binding and is at least partially in labelled form, and a receptor R which consists of a binding partner P1 which is capable of monovalent binding with P2 and of a binding site R1 for the ligand to be determined.
AU71013/91A 1990-02-26 1991-02-12 Method for the immunological determination of ligands Ceased AU647449B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4006054A DE4006054A1 (en) 1990-02-26 1990-02-26 METHOD FOR THE IMMUNOLOGICAL DETERMINATION OF LIGANDS
DE4006054 1990-02-26

Publications (2)

Publication Number Publication Date
AU7101391A AU7101391A (en) 1991-08-29
AU647449B2 true AU647449B2 (en) 1994-03-24

Family

ID=6401009

Family Applications (1)

Application Number Title Priority Date Filing Date
AU71013/91A Ceased AU647449B2 (en) 1990-02-26 1991-02-12 Method for the immunological determination of ligands

Country Status (9)

Country Link
EP (1) EP0444561B1 (en)
JP (1) JP2501960B2 (en)
KR (1) KR940008091B1 (en)
AT (1) ATE145479T1 (en)
AU (1) AU647449B2 (en)
CA (1) CA2034922A1 (en)
DE (2) DE4006054A1 (en)
ES (1) ES2096596T3 (en)
ZA (1) ZA911343B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138251B1 (en) 1996-04-22 2006-11-21 Amgen Inc. Polynucleotides encoding a neurotrophic factor receptor
JP2002505576A (en) * 1997-05-30 2002-02-19 アムジエン・インコーポレーテツド Neurotrophic factor receptor
EP1695083A1 (en) 2003-12-01 2006-08-30 Dade Behring Marburg GmbH Homogeneous detection method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4495296A (en) * 1979-05-21 1985-01-22 New York Blood Center, Inc. Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays
NZ211888A (en) * 1984-05-10 1987-08-31 Abbott Lab Biotin-antibiotin immunoassay for detecting ligands
JPS60252265A (en) * 1984-05-29 1985-12-12 Toray Ind Inc Light emitting reagent and method for measuring biologically active material using said reagent
IE64286B1 (en) * 1988-05-25 1995-07-26 Boehringer Mannheim Gmbh Process for the determination of an immunologically detectable substance and a suitable vessel therefor
DE3834766A1 (en) * 1988-10-12 1990-04-26 Boehringer Mannheim Gmbh METHOD FOR DETERMINING A SPECIFICALLY BINDABLE SUBSTANCE

Also Published As

Publication number Publication date
DE59108355D1 (en) 1997-01-02
AU7101391A (en) 1991-08-29
CA2034922A1 (en) 1991-08-27
ES2096596T3 (en) 1997-03-16
KR940008091B1 (en) 1994-09-02
ZA911343B (en) 1991-11-27
JP2501960B2 (en) 1996-05-29
KR910021586A (en) 1991-12-20
DE4006054A1 (en) 1991-08-29
ATE145479T1 (en) 1996-12-15
EP0444561B1 (en) 1996-11-20
EP0444561A3 (en) 1992-10-14
EP0444561A2 (en) 1991-09-04
JPH04216465A (en) 1992-08-06

Similar Documents

Publication Publication Date Title
AU3090889A (en) Method for determination of a polyvalent substance using an immunoaggregate
ES2001811A6 (en) Solid-phase analytical device and method for using same.
AU517300B2 (en) Specific binding assay method'and reagent means
EP0710293A4 (en) Lyophilized ligand-receptor complexes for assays and sensors
DE3883478D1 (en) Peridinin-chlorophyll complex as a fluorescent identifier.
AU1198895A (en) Sensor device for sandwich assay
EP0452858A3 (en) Metal complexes of acid adducts to dioxime ligands useful in labelling proteins and other amine-containing compounds
DE59409947D1 (en) Reduction of the hook effect in immunoassays with particulate carrier material
DE59007200D1 (en) Method for determining an analyte.
AU2603288A (en) Bidentate conjugate and method of use thereof
ATE67599T1 (en) HETEROGENOUS IMMUNOASSAY.
AU647449B2 (en) Method for the immunological determination of ligands
AU3996693A (en) Process for the immunochemical determination of an analyte
ES8605106A1 (en) Process and reagent for the quantitative determination of free thyroxine in plasma, serum and full blood.
GR3009564T3 (en)
CA2004642A1 (en) Process for the detection of compounds containing carbohydrate and a suitable reagent therefor
NO862430L (en) HISTAMINE DERIVATIVES, IMMUNOGEN CONJUGATES AND ANTIBODIES CREATED AGAINST THESE.
GR3005066T3 (en)
AU1718588A (en) Method for the determination of albumin in biological fluids
DE3172795D1 (en) Process for the determination of ligands, test kit for carrying out the process and its application
EP0276719A3 (en) Immunoassay utilizing microparticle neutralization
AU2868489A (en) Assays for incipient diabetes, monoclonal antibodies used therein and kits for use therefor
DE3468324D1 (en) Specific antibody to the native form of 2'5'-oligonucleotides, the method of preparation and the use as reagents in immunoassays or for binding 2'5'-oligonucleotides in biological systems
DE3569828D1 (en) Method for immobilizing substances in synthetic solid phases, and synthetic solid phases for use in immunosorbent assays
HUT61619A (en) Method for radioimmunological derminic 5-hydroxi- 6,8,11,14-eicosatetraen- acid content of the biological samples