AU603883B2 - Genetic engineering process for the preparation of polypeptides - Google Patents

Genetic engineering process for the preparation of polypeptides Download PDF

Info

Publication number
AU603883B2
AU603883B2 AU14469/88A AU1446988A AU603883B2 AU 603883 B2 AU603883 B2 AU 603883B2 AU 14469/88 A AU14469/88 A AU 14469/88A AU 1446988 A AU1446988 A AU 1446988A AU 603883 B2 AU603883 B2 AU 603883B2
Authority
AU
Australia
Prior art keywords
galactosidase
gene
fragment
polypeptide
codons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU14469/88A
Other languages
English (en)
Other versions
AU1446988A (en
Inventor
Wolfgang Becker
Hans Willi Jansen
Waldemar Wetekam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of AU1446988A publication Critical patent/AU1446988A/en
Application granted granted Critical
Publication of AU603883B2 publication Critical patent/AU603883B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU14469/88A 1987-04-11 1988-04-11 Genetic engineering process for the preparation of polypeptides Ceased AU603883B2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3712361 1987-04-11
DE3712361 1987-04-11
DE3805150 1988-02-19
DE3805150A DE3805150A1 (de) 1987-04-11 1988-02-19 Gentechnologisches verfahren zur herstellung von polypeptiden

Publications (2)

Publication Number Publication Date
AU1446988A AU1446988A (en) 1988-10-13
AU603883B2 true AU603883B2 (en) 1990-11-29

Family

ID=25854588

Family Applications (1)

Application Number Title Priority Date Filing Date
AU14469/88A Ceased AU603883B2 (en) 1987-04-11 1988-04-11 Genetic engineering process for the preparation of polypeptides

Country Status (12)

Country Link
EP (1) EP0286956B1 (enExample)
JP (1) JPS63263087A (enExample)
KR (1) KR880012758A (enExample)
AU (1) AU603883B2 (enExample)
DE (2) DE3805150A1 (enExample)
DK (1) DK196188A (enExample)
FI (1) FI881617A7 (enExample)
GR (1) GR3007417T3 (enExample)
IL (1) IL86023A (enExample)
NO (1) NO881548L (enExample)
NZ (1) NZ224184A (enExample)
PT (1) PT87211B (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK175535B1 (da) * 1987-03-04 2004-11-29 Daiichi Suntory Pharma Co Ltd Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid
DE3716722A1 (de) * 1987-05-19 1988-12-01 Hoechst Ag Gentechnologisches verfahren zur herstellung von angiogeninen
DE3844211A1 (de) * 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
US5527687A (en) * 1993-05-25 1996-06-18 American Cyanamid Company Enzyme induction screen for ergosterol biosynthesis inhibitors
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
PT821006E (pt) 1996-07-26 2004-09-30 Aventis Pharma Gmbh Derivados de insulina com ligacao a zinco aumentada
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
CN105189788B (zh) * 2013-03-12 2018-07-13 纳幕尔杜邦公司 用于鉴定罕见切割工程化双链断裂诱导剂的变体识别位点的方法及其组合物和用途
KR20200080748A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
KR20200080747A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1563188A (en) * 1987-05-06 1988-11-10 Rudolf Glockshuber A process for the preparation of a genetically encodable polypeptide

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2008123B (en) * 1977-11-08 1982-09-08 Genentech Inc Method of means for microbial polypeptide expression
JPS6028994A (ja) * 1983-07-08 1985-02-14 Wakunaga Seiyaku Kk 〔２１―ロイシン〕ヒトウロガストロン
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
DK175535B1 (da) * 1987-03-04 2004-11-29 Daiichi Suntory Pharma Co Ltd Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1563188A (en) * 1987-05-06 1988-11-10 Rudolf Glockshuber A process for the preparation of a genetically encodable polypeptide

Also Published As

Publication number Publication date
PT87211B (pt) 1992-07-31
FI881617A7 (fi) 1988-10-12
FI881617A0 (fi) 1988-04-07
DK196188A (da) 1988-10-12
KR880012758A (ko) 1988-11-29
EP0286956B1 (de) 1992-12-30
NO881548L (no) 1988-10-12
EP0286956A3 (en) 1989-04-05
GR3007417T3 (enExample) 1993-07-30
AU1446988A (en) 1988-10-13
EP0286956A2 (de) 1988-10-19
PT87211A (pt) 1988-05-01
DE3877035D1 (de) 1993-02-11
NZ224184A (en) 1989-10-27
NO881548D0 (no) 1988-04-08
IL86023A0 (en) 1988-09-30
IL86023A (en) 1993-01-31
JPS63263087A (ja) 1988-10-31
DK196188D0 (da) 1988-04-11
DE3805150A1 (de) 1988-10-20

Similar Documents

Publication Publication Date Title
JP2566933B2 (ja) 真核細胞融合タンパク質、その生産及び用途、並びに方法
AU648670B2 (en) A-C-B Proinsulin, method of manufacturing and using same, and intermediates in insulin production
CA1336329C (en) Fusion proteins, a process for their preparation and their use
Engler et al. Cloning of authentic human epidermal growth factor as a bacterial secretory protein and its initial structure-function analysis by site-directed mutagenesis.
EP0489780A4 (en) Fusion proteins, their preparation and use
IE53897B1 (en) Methods and products for facile microbial expression of dna sequences
FI93734B (fi) Menetelmä eukaryoottisen painolastiosan sisältävien fuusioproteiinien valmistamiseksi ja menetelmässä käyttökelpoisia tuotteita
WO1992002550A1 (en) Expression of recombinant polypeptides with improved purification
AU614933B2 (en) Functional DNA block and plasmid coding for hirudin, transformed yeast, method for preparing hirudin, hirudin obtained and its pharmaceutical use
EP0470165A1 (en) Fusion proteins containing n-terminal fragments of human serum albumin
EP0205475B1 (en) Recombinant methods for production of serine protease inhibitors and dna sequences useful for same
Zhu et al. Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin. Requirement of a carboxypeptidase B-like activity for maturation.
AU595805B2 (en) Releasing desired peptides from fusion polypeptides using endopeptidases
US5087564A (en) Release of recombinant peptides from polypeptides using V8 endopeptidase
US5420242A (en) Production of human parathyroid hormone from microorganisms
WO1989003886A1 (en) Expression systems for preparation of polypeptides in prokaryotic cells
CA2040810C (en) Process for producing foreign proteins in streptomyces
JPS63263087A (ja) ポリペプチドの遺伝子工学的製造法
AU655316B2 (en) Increased expression of low molecular weight recombinant polypeptides
US6194200B1 (en) Expression systems for preparation of polypeptides in prokaryotic cells
US5426036A (en) Processes for the preparation of foreign proteins in streptomycetes
FI97549B (fi) Menetelmä vierasproteiinien valmistamiseksi Streptomycessoluissa
Šebo et al. Delineation of the minimal portion of the Bacillus sphaericus 1593M toxin required for the expression of larvicidal activity
Lukacsovich et al. A family of expression vectors based on the rrnBP2 promoter of Escherichia coli
Daum et al. Production of human adrenocorticotropin by cleavage of alkaline‐phosphatase‐derived fusion proteins containing repetitive recognition sequences for collagenases