AU597616B2 - Pregnancy test with epf - Google Patents
Pregnancy test with epf Download PDFInfo
- Publication number
- AU597616B2 AU597616B2 AU68174/87A AU6817487A AU597616B2 AU 597616 B2 AU597616 B2 AU 597616B2 AU 68174/87 A AU68174/87 A AU 68174/87A AU 6817487 A AU6817487 A AU 6817487A AU 597616 B2 AU597616 B2 AU 597616B2
- Authority
- AU
- Australia
- Prior art keywords
- epf
- female
- cells
- cell
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000009597 pregnancy test Methods 0.000 title description 3
- 210000004027 cell Anatomy 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 34
- 239000001301 oxygen Substances 0.000 claims abstract description 14
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 14
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 12
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 7
- 150000003254 radicals Chemical class 0.000 claims description 19
- 238000005259 measurement Methods 0.000 claims description 18
- 230000015572 biosynthetic process Effects 0.000 claims description 15
- 210000005087 mononuclear cell Anatomy 0.000 claims description 15
- -1 oxygen radicals Chemical class 0.000 claims description 14
- 239000000523 sample Substances 0.000 claims description 11
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 claims description 10
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- 230000009027 insemination Effects 0.000 claims description 6
- 230000004720 fertilization Effects 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 4
- 239000013068 control sample Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000002644 phorbol ester Substances 0.000 claims description 3
- 210000001132 alveolar macrophage Anatomy 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 239000003599 detergent Substances 0.000 claims description 2
- 239000002555 ionophore Substances 0.000 claims description 2
- 230000000236 ionophoric effect Effects 0.000 claims description 2
- 210000002540 macrophage Anatomy 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims 3
- 230000035935 pregnancy Effects 0.000 abstract description 17
- 238000002360 preparation method Methods 0.000 abstract description 12
- 210000004369 blood Anatomy 0.000 abstract description 11
- 239000008280 blood Substances 0.000 abstract description 11
- 230000005281 excited state Effects 0.000 abstract description 11
- 210000002700 urine Anatomy 0.000 abstract description 11
- 238000001514 detection method Methods 0.000 abstract description 8
- 230000002710 gonadal effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 102100024341 10 kDa heat shock protein, mitochondrial Human genes 0.000 description 34
- 108010046025 early pregnancy factor Proteins 0.000 description 34
- 238000012546 transfer Methods 0.000 description 18
- 210000002966 serum Anatomy 0.000 description 16
- 238000012360 testing method Methods 0.000 description 14
- 210000001161 mammalian embryo Anatomy 0.000 description 11
- 239000012981 Hank's balanced salt solution Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- LEOJISUPFSWNMA-UHFFFAOYSA-N ABEI Chemical compound O=C1NNC(=O)C=2C1=CC(N(CCCCN)CC)=CC=2 LEOJISUPFSWNMA-UHFFFAOYSA-N 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- KNJDBYZZKAZQNG-UHFFFAOYSA-N lucigenin Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.C12=CC=CC=C2[N+](C)=C(C=CC=C2)C2=C1C1=C(C=CC=C2)C2=[N+](C)C2=CC=CC=C12 KNJDBYZZKAZQNG-UHFFFAOYSA-N 0.000 description 3
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- HUDPLKWXRLNSPC-UHFFFAOYSA-N 4-aminophthalhydrazide Chemical compound O=C1NNC(=O)C=2C1=CC(N)=CC=2 HUDPLKWXRLNSPC-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000018832 Cytochromes Human genes 0.000 description 2
- 108010052832 Cytochromes Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 2
- 230000005283 ground state Effects 0.000 description 2
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- RODXRVNMMDRFIK-UHFFFAOYSA-N scopoletin Chemical compound C1=CC(=O)OC2=C1C=C(OC)C(O)=C2 RODXRVNMMDRFIK-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- XDFNWJDGWJVGGN-UHFFFAOYSA-N 2-(2,7-dichloro-3,6-dihydroxy-9h-xanthen-9-yl)benzoic acid Chemical compound OC(=O)C1=CC=CC=C1C1C2=CC(Cl)=C(O)C=C2OC2=CC(O)=C(Cl)C=C21 XDFNWJDGWJVGGN-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- BEADQYOTAORBKI-UHFFFAOYSA-N 6-[6-aminohexyl(ethyl)amino]-2,3-dihydrophthalazine-1,4-dione Chemical compound O=C1NNC(=O)C=2C1=CC(N(CCCCCCN)CC)=CC=2 BEADQYOTAORBKI-UHFFFAOYSA-N 0.000 description 1
- ORILYTVJVMAKLC-UHFFFAOYSA-N Adamantane Natural products C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- XEHFSYYAGCUKEN-UHFFFAOYSA-N Dihydroscopoletin Natural products C1CC(=O)OC2=C1C=C(OC)C(O)=C2 XEHFSYYAGCUKEN-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108010035746 Pregnancy Proteins Proteins 0.000 description 1
- 102000008217 Pregnancy Proteins Human genes 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000272534 Struthio camelus Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 208000024313 Testicular Neoplasms Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000002429 anti-coagulating effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 210000003293 antilymphocyte serum Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000804 electron spin resonance spectroscopy Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002433 mononuclear leukocyte Anatomy 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 150000004633 phorbol derivatives Chemical class 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- FWYIBGHGBOVPNL-UHFFFAOYSA-N scopoletin Natural products COC=1C=C2C=CC(OC2=C(C1)O)=O FWYIBGHGBOVPNL-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002381 testicular Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3603053 | 1986-01-30 | ||
| DE19863603053 DE3603053A1 (de) | 1986-01-30 | 1986-01-30 | Schwangerschaftsnachweis mit epf |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU6817487A AU6817487A (en) | 1987-08-06 |
| AU597616B2 true AU597616B2 (en) | 1990-06-07 |
Family
ID=6293121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU68174/87A Ceased AU597616B2 (en) | 1986-01-30 | 1987-01-30 | Pregnancy test with epf |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4877742A (https=) |
| EP (1) | EP0232216B1 (https=) |
| JP (1) | JPS62183751A (https=) |
| AT (1) | ATE71461T1 (https=) |
| AU (1) | AU597616B2 (https=) |
| DE (2) | DE3603053A1 (https=) |
| ES (1) | ES2038203T3 (https=) |
| GR (1) | GR3003803T3 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1337394C (en) * | 1987-11-17 | 1995-10-24 | Nelson N. H. Teng | Vaginal sample test and reagents |
| AU3511900A (en) * | 1999-03-02 | 2000-09-21 | Kems Bio-Test Ltd. | Bovine pregnancy testing |
| US20030059951A1 (en) * | 2001-08-14 | 2003-03-27 | Frushour Susan L.M. | Maternal status testing in animals |
| US7393696B2 (en) * | 2001-09-28 | 2008-07-01 | Aspenbio Pharma, Inc. | Bovine pregnancy test |
| EP1546716A4 (en) * | 2002-05-02 | 2007-04-11 | Aspenbio Inc | PREGNANCY PROOF |
| US8426151B2 (en) * | 2010-02-10 | 2013-04-23 | Companion Diagnostics Inc. | Systems and methods for the detection of biomarkers |
| CN118962159A (zh) * | 2024-08-07 | 2024-11-15 | 吉林农业大学 | 一种基于体液诊断绵羊早期妊娠的方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5589786A (en) * | 1985-03-12 | 1986-10-13 | University Of Queensland, The | Method for detecting early pregnancy factor (epf) in mammals,purifying epf and method for producing a monoclonal antibody |
| AU567747B2 (en) * | 1983-07-21 | 1987-12-03 | Idaho Research Foundation Inc., The | Early detection of mammalian pregnancy |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4139348A (en) * | 1975-11-28 | 1979-02-13 | Massachusetts Institute Of Technology | Electrochemical process and apparatus to control the chemical state of a material |
| JPS57502137A (https=) * | 1980-12-22 | 1982-12-02 | ||
| US4543339A (en) * | 1982-03-16 | 1985-09-24 | Oneill Christopher | Early pregnancy detection by detecting enhanced blood platelet activation |
| FR2544080B1 (fr) * | 1983-04-06 | 1986-03-14 | Centre Nat Rech Scient | Procede de detection et de mesure par fluorescence d'une endocytose cellulaire dependante de proteines de la membrane cellulaire |
| WO1986005498A1 (en) * | 1985-03-12 | 1986-09-25 | University Of Queensland | Method for detecting early pregnancy factor (epf) in mammals, purifying epf and method for producing a monoclonal antibody |
-
1986
- 1986-01-30 DE DE19863603053 patent/DE3603053A1/de not_active Withdrawn
-
1987
- 1987-01-29 AT AT87730009T patent/ATE71461T1/de not_active IP Right Cessation
- 1987-01-29 DE DE8787730009T patent/DE3775784D1/de not_active Expired - Lifetime
- 1987-01-29 EP EP87730009A patent/EP0232216B1/de not_active Expired - Lifetime
- 1987-01-29 US US07/008,239 patent/US4877742A/en not_active Expired - Fee Related
- 1987-01-29 ES ES198787730009T patent/ES2038203T3/es not_active Expired - Lifetime
- 1987-01-30 AU AU68174/87A patent/AU597616B2/en not_active Ceased
- 1987-01-30 JP JP62018808A patent/JPS62183751A/ja active Pending
-
1992
- 1992-02-13 GR GR920400219T patent/GR3003803T3/el unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU567747B2 (en) * | 1983-07-21 | 1987-12-03 | Idaho Research Foundation Inc., The | Early detection of mammalian pregnancy |
| AU5589786A (en) * | 1985-03-12 | 1986-10-13 | University Of Queensland, The | Method for detecting early pregnancy factor (epf) in mammals,purifying epf and method for producing a monoclonal antibody |
Also Published As
| Publication number | Publication date |
|---|---|
| AU6817487A (en) | 1987-08-06 |
| US4877742A (en) | 1989-10-31 |
| DE3603053A1 (de) | 1987-08-06 |
| ES2038203T3 (es) | 1993-07-16 |
| DE3775784D1 (de) | 1992-02-20 |
| JPS62183751A (ja) | 1987-08-12 |
| EP0232216A2 (de) | 1987-08-12 |
| EP0232216A3 (en) | 1988-06-01 |
| GR3003803T3 (https=) | 1993-03-16 |
| ATE71461T1 (de) | 1992-01-15 |
| EP0232216B1 (de) | 1992-01-08 |
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