AU5558300A - Derivatives of 4'-demycarosyl-8a-aza-8a-homotylosin - Google Patents
Derivatives of 4'-demycarosyl-8a-aza-8a-homotylosin Download PDFInfo
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- AU5558300A AU5558300A AU55583/00A AU5558300A AU5558300A AU 5558300 A AU5558300 A AU 5558300A AU 55583/00 A AU55583/00 A AU 55583/00A AU 5558300 A AU5558300 A AU 5558300A AU 5558300 A AU5558300 A AU 5558300A
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- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 75
- 150000001875 compounds Chemical class 0.000 claims description 72
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 36
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 claims description 20
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 12
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 12
- 238000006140 methanolysis reaction Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 11
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 claims description 8
- 150000001241 acetals Chemical class 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 7
- VHYFNPMBLIVWCW-UHFFFAOYSA-N 4-Dimethylaminopyridine Chemical compound CN(C)C1=CC=NC=C1 VHYFNPMBLIVWCW-UHFFFAOYSA-N 0.000 claims description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 230000006179 O-acylation Effects 0.000 claims description 4
- 150000008064 anhydrides Chemical class 0.000 claims description 4
- 239000003054 catalyst Substances 0.000 claims description 4
- 238000007254 oxidation reaction Methods 0.000 claims description 4
- 229940086542 triethylamine Drugs 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- NRTYMEPCRDJMPZ-UHFFFAOYSA-N pyridine;2,2,2-trifluoroacetic acid Chemical compound C1=CC=NC=C1.OC(=O)C(F)(F)F NRTYMEPCRDJMPZ-UHFFFAOYSA-N 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 239000012442 inert solvent Substances 0.000 claims description 2
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims description 2
- 150000007530 organic bases Chemical class 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 claims description 2
- 239000000047 product Substances 0.000 description 41
- 238000005481 NMR spectroscopy Methods 0.000 description 32
- 239000000243 solution Substances 0.000 description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 17
- 239000000741 silica gel Substances 0.000 description 17
- 229910002027 silica gel Inorganic materials 0.000 description 17
- 229960001866 silicon dioxide Drugs 0.000 description 17
- 238000003818 flash chromatography Methods 0.000 description 16
- 239000002904 solvent Substances 0.000 description 16
- SPEUIVXLLWOEMJ-UHFFFAOYSA-N 1,1-dimethoxyethane Chemical compound COC(C)OC SPEUIVXLLWOEMJ-UHFFFAOYSA-N 0.000 description 15
- 238000002955 isolation Methods 0.000 description 13
- 239000012043 crude product Substances 0.000 description 12
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 239000011541 reaction mixture Substances 0.000 description 6
- 238000005160 1H NMR spectroscopy Methods 0.000 description 5
- 125000002252 acyl group Chemical group 0.000 description 5
- 229920006395 saturated elastomer Polymers 0.000 description 5
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 3
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 3
- DTQVDTLACAAQTR-UHFFFAOYSA-M Trifluoroacetate Chemical compound [O-]C(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-M 0.000 description 3
- 229940125904 compound 1 Drugs 0.000 description 3
- 229940126214 compound 3 Drugs 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 125000006297 carbonyl amino group Chemical group [H]N([*:2])C([*:1])=O 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 229940125782 compound 2 Drugs 0.000 description 2
- 229940125898 compound 5 Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- GHYOCDFICYLMRF-UTIIJYGPSA-N (2S,3R)-N-[(2S)-3-(cyclopenten-1-yl)-1-[(2R)-2-methyloxiran-2-yl]-1-oxopropan-2-yl]-3-hydroxy-3-(4-methoxyphenyl)-2-[[(2S)-2-[(2-morpholin-4-ylacetyl)amino]propanoyl]amino]propanamide Chemical compound C1(=CCCC1)C[C@@H](C(=O)[C@@]1(OC1)C)NC([C@H]([C@@H](C1=CC=C(C=C1)OC)O)NC([C@H](C)NC(CN1CCOCC1)=O)=O)=O GHYOCDFICYLMRF-UTIIJYGPSA-N 0.000 description 1
- QFLWZFQWSBQYPS-AWRAUJHKSA-N (3S)-3-[[(2S)-2-[[(2S)-2-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-methylbutanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-[1-bis(4-chlorophenoxy)phosphorylbutylamino]-4-oxobutanoic acid Chemical compound CCCC(NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)CCCCC1SC[C@@H]2NC(=O)N[C@H]12)C(C)C)P(=O)(Oc1ccc(Cl)cc1)Oc1ccc(Cl)cc1 QFLWZFQWSBQYPS-AWRAUJHKSA-N 0.000 description 1
- IWZSHWBGHQBIML-ZGGLMWTQSA-N (3S,8S,10R,13S,14S,17S)-17-isoquinolin-7-yl-N,N,10,13-tetramethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-amine Chemical compound CN(C)[C@H]1CC[C@]2(C)C3CC[C@@]4(C)[C@@H](CC[C@@H]4c4ccc5ccncc5c4)[C@@H]3CC=C2C1 IWZSHWBGHQBIML-ZGGLMWTQSA-N 0.000 description 1
- NPHULPIAPWNOOH-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-3-(2,3-dihydroindol-1-ylmethyl)pyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C=1C(=NN(C=1)CC(=O)N1CC2=C(CC1)NN=N2)CN1CCC2=CC=CC=C12 NPHULPIAPWNOOH-UHFFFAOYSA-N 0.000 description 1
- HVTQDSGGHBWVTR-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-3-phenylmethoxypyrazol-1-yl]-1-morpholin-4-ylethanone Chemical compound C(C1=CC=CC=C1)OC1=NN(C=C1C=1C=NC(=NC=1)NC1CC2=CC=CC=C2C1)CC(=O)N1CCOCC1 HVTQDSGGHBWVTR-UHFFFAOYSA-N 0.000 description 1
- VXZBYIWNGKSFOJ-UHFFFAOYSA-N 2-[4-[5-(2,3-dihydro-1H-inden-2-ylamino)pyrazin-2-yl]pyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC=1N=CC(=NC=1)C=1C=NN(C=1)CC(=O)N1CC2=C(CC1)NN=N2 VXZBYIWNGKSFOJ-UHFFFAOYSA-N 0.000 description 1
- -1 3-dimethylamino-propyl Chemical group 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- WTFUTSCZYYCBAY-SXBRIOAWSA-N 6-[(E)-C-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-N-hydroxycarbonimidoyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C/C(=N/O)/C1=CC2=C(NC(O2)=O)C=C1 WTFUTSCZYYCBAY-SXBRIOAWSA-N 0.000 description 1
- 229910017974 NH40H Inorganic materials 0.000 description 1
- 238000003833 Wallach reaction Methods 0.000 description 1
- WREOTYWODABZMH-DTZQCDIJSA-N [[(2r,3s,4r,5r)-3,4-dihydroxy-5-[2-oxo-4-(2-phenylethoxyamino)pyrimidin-1-yl]oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1N(C=C\1)C(=O)NC/1=N\OCCC1=CC=CC=C1 WREOTYWODABZMH-DTZQCDIJSA-N 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 239000002026 chloroform extract Substances 0.000 description 1
- 229940125797 compound 12 Drugs 0.000 description 1
- 229940125758 compound 15 Drugs 0.000 description 1
- 229940125810 compound 20 Drugs 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 125000004005 formimidoyl group Chemical group [H]\N=C(/[H])* 0.000 description 1
- JAXFJECJQZDFJS-XHEPKHHKSA-N gtpl8555 Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@@H]1C(=O)N[C@H](B1O[C@@]2(C)[C@H]3C[C@H](C3(C)C)C[C@H]2O1)CCC1=CC=C(F)C=C1 JAXFJECJQZDFJS-XHEPKHHKSA-N 0.000 description 1
- 150000008282 halocarbons Chemical class 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000006268 reductive amination reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Oncology (AREA)
- Animal Behavior & Ethology (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Cephalosporin Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Saccharide Compounds (AREA)
Description
WO 00/77016 PCT/HROO/00018 1 DERIVATIVES OF 4'-DEMYCAROSYL-8a-AZA-8a-HOMOTYLOSIN Technical Field IPC: A 61 K 31/70 C 07 H 17/08 Technical Problem The present invention relates to novel compounds from the class of 17-membered azalides having an antibacterial action. More particularly, the invention relates to derivatives of 4'-demycarosyl-8a-aza-8a-homotylosin of the formula I CO-NH CH3 8a R 20
H
3C
R
2 H3C RRN(H 3
)
2 OH H 3 3 R OCH O R5'
CH
3 wherein R represents CHO, CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' represents H or C 1
-C
3 acyl,
R
2 represents OR 6 and R 6 represents H or C 1
-C
3 acyl,
R
3 represents H or R 2 and R 3 together represent =0, R represents OH, R5 represents H or R 4 and R 5 together represent =0, and to a process for the preparation thereof. Prior Art WO 00/77016 PCT/HROO/00018 4'-Demycarosyl-8a-aza-8a-homotylosim, a novel semisynthetic macrolide from the class of 17-membered azalides, was prepared by a double transformation of C-9 ketone of the 16-membered antibiotic 4'-demycarosyl-tylosin (R. L. Hamill, Antibiotics and Chemotherapy 11, 328 (1961); A. Narandja et al, EP 0 287 082 B1; N. Lopotar et al, EP 0 410 433 B1). By reductive amination of C-20 aldehyde group in the presence of formic acid (Wallach reaction, J. March: "Advanced Organic Chemistry", third ed. 6-15 p. 799 Wiley, New York, 1985) there was prepared 4' demycarosyl-20-deoxo-20-dibenzylamino-8a-aza-8a-homotylosin (N. Lopotar, HR Patent Application P940962A, 30.11.1994).
CI-C
3 acyl esters of 4'-demycarosyl-8a-aza-8a-homotylosin and of 4'-demycarosyl 20-deoxo-20-dibenzylamino-8 a-aza-8a-homotylosin as well as 4"-deoxy-4"-oxo- and 3-deoxy-3-oxo derivatives of 4'-demycarosyl-8 a-aza-8 a-homotylosin and of 4' demycarosyl-20-deoxo-20-dibenzylamino-8 a-aza-8 a-homotylosin, C 1
-C
3 acyl esters thereof and a process for the preparation thereof have hitherto not been disclosed in Prior Art. Detailed Description of the Invention According to the present invention derivatives of 4'-demycarosyl-8a-aza-8a homotylosin of the formula I WO 00/77016 PCT/HR0O/00018 3 CO-NH CH3 8a R 20
H
3C
R
2
H
3 0
N(CH
3
)
2
H
3 RZ0p OR1 411 0" 0OCH 3 3
OCH
3 00R 5
CH
3 wherein R represents CHO, CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 ,
R
1 represents H or C 1
-C
3 acyl,
R
2 represents OR 6 and R 6 represents H or Ci-C 3 acyl,
R
3 represents H or R 2 and R 3 together represent =0, R represents OH, R5 represents H or R 4 and R 5 together represent =0, may be prepared in such a way that 4'-demycarosyl-8a-aza-8a-homotylosin 20-dimethylacetal of the formula Ila and 4' demycarosyl-20-deoxo-20-dibenzylamino-8a-aza-8a-homotylosin of the formula IIb CO-NH CH3 8a R
H
3 0
H
3 O 3 OH N(OH 3
)
2 HO 4'HO HO O 3 OH 3 OCH3 3 OH
CH
3 Ila R = CH(OCH 3
)
2 Ilb R = CH 2
N[CH
2
(C
6
H
5
)]
2 WO 00/77016 PCT/HROO/00018 4 are subjected to A) an O-acylation with anhydrides of C 1
-C
3 carboxylic acids, preferably with acetic acid anhydride in methylene chloride during 15 minutes to 1 hour at room temperature, and the obtained compounds of the formula I, wherein R represents 2 6
CH(OCH
3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' represents COCH 3 , R2 represents OR , wherein R 6 represents H, R 3 and R' are the same and represent H and R 4 represents OH, are optionally subjected to Al) an O-acylation with anhydrides of C 1
-C
3 carboxylic acids, preferably with acetic acid anhydride in methylene chloride in the presence of an organic base, preferably triethyl amine and 4-dimethylaniminopyridine as a catalyst during 30 hours at room temperature, and the obtained compounds of the formula I, wherein R represents 2 6
CH(OCH
3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' represents COCH 3 , R represents OR , wherein R 6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH, are optionally subjected to B) an oxidation reaction with N(3-dimethylamino-propyl)-N'ethyl carbodilmide hydrochloride in the presence of dimethylsulfoxide and pyridine trifluoroacetate as a catalyst in an inert solvent, preferably methylene chloride, during 2 to 6 hours at a temperature from 10'C to room temperature, and the obtained compounds of the formula I, wherein R represents CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' represents 26 63
COCH
3 , R 2 represents OR , wherein R represents COCH 3 , R3 represents H and R 4 and R 5 together represent =O, are optionally subjected to C) methanolysis at room temperature for 2 days and the obtained compounds of the formula I, wherein R represents CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents COCH 3 , and R and
R
5 together represent =0, WO 00/77016 PCT/HROO/00018 5 are optionally subjected to Cl) an alkaline methanolysis in a mixture of methanol and 25% ammonia (4:1) at a temperature from 5'C to room temperature during 20 to 60 hours to obtain compounds of the formula I, wherein R represents CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and
R
5 together represent =0; or the compound obtained according to process C 1 of the formula I, wherein R represents CH(OCH 3
)
2 , R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and R together represent =0, is optionally subjected to D) a hydrolysis of the acetal in a mixture of acetonitrile and 0.1 N hydrochloric acid (1:1) for 2 hours at room temperature to obtain the compound of the formula I, wherein R represents a CHO group, R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and R 5 together represent =0; or compounds obtained according to process A of the formula I, wherein R represents CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R' represents COCH 3 , R 2 represents OR 6 , wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH, are optionally subjected to oxidation in the manner disclosed in B, and the obtained compounds of the formula I, wherein R represents CH(OCH 3
)
2 or
CH
2
N[CH
2
(C
6
H
5
)]
2 , R1 represents COCH 3 , R 2 and R 3 together represent =0, R 4 represents OH and R 5 represents H, are optionally subjected to methanolysis in the manner disclosed in C, WO 00/77016 PCT/HR0O/00018 6 to obtain compounds of the formula I, wherein R represents CH(OCH 3
)
2 or CH2N[CH 2
(C
6
H
5
)]
2 , RI and R 5 are the same and represent H, R 2 and R 3 together represent =O and R 4 represents OH; or the compound obtained according to process B of the formula I, wherein R represents a CH(OCH 3
)
2 group, R' represents COCH 3 , R 2 and R 3 together represent =O, R 4 represents OH and R 5 represents H, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, and the obtained compound of the formula I, wherein R represents a CHO group, R1 represents COCH 3 , R 2 and R 3 together represent =O, R 4 represents OH and R 5 represents H, is optionally subjected to methanolysis in the manner disclosed in C, to obtain the compound of the formula I, wherein R represents a CHO group, R' and R are the same and represent H, R 2 and R 3 together represent =O and R 4 represents OH; or the compound obtained according to process A of the formula I, wherein R represents CH(OCH 3
)
2 , R' represents COCH 3 , R 2 represents OR 6 , wherein R 6 represents H, R 3 and R 5 are the same and represent H and represents OH, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, to obtain a compound of the formula I wherein R represents CHO, R1 represents
COCH
3 , R 2 represents OR6, wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH; or compounds obtained according to process Al of the formula I, wherein R represents CH(OCH 3
)
2 or CH 2
N[CH
2
(C
6
H
5
)]
2 , R1 represents COCH 3 , R 2 represents OR 6 , wherein R 6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH, WO 00/77016 PCT/HROO/00018 7 are optionally subjected to methanolysis in the manner disclosed in C, to obtain compounds of the formula I, wherein R represents CH(OCH 3
)
2 or
CH
2
N[CH
2
(C
6
H
5
)]
2 , R1, R3 and R 5 are the same and represent H, R 2 represents OR , wherein R6 represents COCH 3 , and R4 represents OH; or the compound obtained according to process Al of the formula I, wherein R represents CH(OCH 3
)
2 , R 1 represents COCH 3 , R 2 represents OR 6 , wherein R6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, and the obtained compound of the formula I, wherein R represents CHO, R 1 represents COCH 3 , R2 represents OR 6 , wherein R 6 represents COCH 3 , R3 and R 5 are the same and represent H and R4 represents OH, is optionally subjected to methanolysis in the manner disclosed in C, to obtain the compound of the formula I. wherein R represents CHO, R 1 , R 3 and R 5 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents COCH 3 , and
R
4 represents OH. According to the present invention novel compouds are isolated by conventional processes of extraction from aqueous solutions of halogenated hydrocarbons such as methylene chloride or chloroform and by evaporating the organic solvent to a dry residue. Optionally, the separation of the reaction products or the purification of the products for spectral analyses is carried out by flash chromatography on a silica gel column (Merck & Co., Silicagel 60, 230-400 meshi/ASTM) in a solvent sistem:
CH
2 Cl 2
-CH
3 OH-conc. NH40H (90:9:1.5, system A), CH 2 Cl 2
-CH
3 OH (90:9, system B) or CHCl 3
-CH
3
COCH
3 (7:3, system G). The structure of the novel compounds was confirmed by spectrometric methods and mass analysis.
WO 00/77016 PCT/HROO/00018 8 The novel compounds show antibacterial action and may be also used as intermediates for preparing novel 17-membered azalide antibiotics. The invention is illustrated and in no way limited by the following Examples.
WO 00/77016 PCT/HROO/00018 9 Example ] 4'-Demycarosyl-2',4'-di-O-acetyl-8a-aza-8a-homotylosin 20-dimethylacetal (1) 4'-Demycarosyl-8a-aza-8a-homotylosim 20-dimethylacetal (5.0 g, 6.02 mmol) was dissolved in dry methylene chloride (50 ml), acetic anhydride (2.0 ml) was added and it was stirred for 15 minutes at room temperature. The reaction mixture was poured into a water/ice mixture (500 ml) and extracted twice with methylene chloride at pH 8.5. The combined organic extracts were washed with a saturated NaHCO 3 solution and water, dried (K 2 C0 3 ) and evaporated at reduced pressure to give a TLC homogeneous product (1) (5.38 g; 97.8 %). TLC: Rf (B) 0.44; Rf (C) 0.22. IR (KBr) cm-1 1749, 1657, 1620, 1544, 1455, 1375, 1229, 1170, 1063. 'H NMR (CDCl 3 ) 5 ppm 7.16 (H-11), 5.69 (H-10), 5.66 (H-13), 4.96 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.76 (H-4'), 4.63 (H-20), 4.58 (H-i"), 4.33 (H-l'), 4.17 (H-8), 3.61 (3"-OCH 3 ), 3.47 (2"-OCH 3 ), 3.56 (2x20-OCH 3 ), 2.33 /3'-N(CH 3
)
2 /, 2.05 (COCH 3 ), 2.03 (COCH 3 ), 1.74 (H-22), 1.17 (H-21). 13 C NMR (CDCl 3 ) 8 ppm 179.1 (C-1), 169.8, 169.4 (2xCOCH 3 ), 166.2 (9-CONH), 144.7 (C-11), 138.2 (C-13), 134.9 (C-12), 119.2 (C-10), 103.5 (C-20), 102.0 (C-l'), 100.9 (C-i"), 72.5 (C-4"), 71.4 (C-4'), 70.3 (C-2'), 65.6 (C-3), 61.5 (3"-OCH 3 ), 59.4 (2"-OCH 3 ), 50.4 (2x20-OCH 3 ), 42.7 (C-8), 42.5 (C-4), 41.0 /3'-N(CH 3
)
2 /, 40.5 (C-2), 34.3 (C-19), 21.8, 20.9 (2xCOCH 3 ), 21.9 (C-21), 12.6 (C-22), 8.3 (C-18). FAB (MH-) 917. Example 2 4'-Demycarosyl-2',4'-di-O-acetyl-20-deoxo-20-dibenzylamino-8a-aza-8a homotylosin (2) WO 00/77016 PCT/HROO/00018 10 4'-Demycarosyl-20-deoxo-20-dibenzylamino-8a-aza-8a-homotylosin (2.8 g, 2.90 mmol) was dissolved in dry methylene chloride (30 ml), acetic anhydride (1.3 ml, 13.76 mmol) was added and it was stirred for 15 minutes at room temperature. The reaction mixture was poured into a water/ice mixture (300 ml) and extracted twice with methylene chloride at pH 6.5. The combined organic extracts were washed with a saturated NaHCO 3 solution and water, dried (K 2 C0 3 ) and evaporated at reduced pressure to give a TLC homogeneous product (2) (3.02 g; 98.9 %). TLC: Rf (B) 0.38; Rf (C) 0.23. IR (KBr) cm' 1749, 1651, 1633, 1548, 1454, 1374, 1231, 1169, 1059. H NMR (CDC 3 ) 6 ppm 7.25 ~ 7.41 (phenyl), 7.10 (H- 11), 5.70 (H-13), 5.65 (H-10), 4.89 (8a-NH) exchangeable with D20, 4.84 (H-2'), 4.74 (H-4'), 4.60 (H-l"), 4.15 (H-l'), 3.62 (3"-OCH 3 ), 3.61 (20-N-CH 2 -phenyl), 3.58 (20-CH 2 -phenyl), 3.51 (2"-OCH 3 ), 2.32 /3'-N(CH 3
)
2 /, 2.06 (COCH 3 ), 2.00 (COCH 3 ), 1.72 (H 22), 1.12 (H-21). 13 C NMR (CDCl 3 ) 6 ppm 173.4 (C-1), 169.9, 169.5 (2xCOCH 3 ), 166.1 (9-CONH), 144.8 (C-11), 137.9 (C-13), 135.2 (C-12), 119.3 (C-10), 102.3 (C-l'), 101.0 (C-i"), 72.5 (C-4"), 71.4 (C-4'), 70.4 (C-2'), 66.0 (C-3), 61.5 (3"-OCH 3 ), 59.5 (2"-OCH 3 ), 52.2 (C-20), 42.9 (C-8), 42.4 (C-4), 41.0 /3'-N(CH 3
)
2 /, 38.7 (C-2), 29.4 (C-19), 21.8 (C-21), 21.1, 21.0 (2xCOCH 3 ), 12.7 (C-22), 8.4 (C-18), 20-N(CH 2
C
6
H
5
)
2 , 139.8, 129.1, 128.0, 126.6, 57.9. FAB (MH*) 1052. Example 3 4'-Demycarosyl-2',4',4"-tri-O-acetyl-8a-aza-8a-homotylosin 20-dimethylacetal (3) Compound 1 (4.0 g, 4.37 mmol) was dissolved in dry methylene chloride (100 ml), triethyl amine (7.0 mil), 4-dimethylaminopyridine (0.12 g) and acetic anhydride (0.42 ml, 4.45 mmol) were added and then the reaction solution was left to stand for 26 WO 00/77016 PCT/HROO/00018 11 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 1 to give a TLC homogeneous product (3) (4.08 g; 97.7 %). TLC: Rf (A) 0.65; Rf (C) 0.54. IR (KBr) cm- 1 1749, 1655, 1618, 1546, 1454, 1374, 1233, 1171, 1052. 'H NMR (CDC 3 ) 8 ppm 7.16 (H-11), 5.69 (H-10), 5.65 (H-13), 4.89 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.76 (H-4'), 4.64 (H-i"), 4.59 (H-20), 4.33 (H-l'), 4.18 (H-8), 3.52 (3"-OCH 3 ), 3.46 (2"OCH 3 ), 3.36 (20-OCH 3 ), 3.35 (20-OCH 3 ), 2.33 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 2.05 (COCH 3 ), 2.03
(COCH
3 ), 1.74 (H-22), 1.16 (H-21). 3 C NMR (CDCl 3 ) 6 ppm 173.1 (C-1), 170.1, 169.8, 169.4 (3xCOCH 3 ), 166.1 (9-CONH), 144.7 (C-11), 138.0 (C-13), 134.9 (C-12), 119.2 (C-10), 103.7 (C-20), 102.1 (C-i'), 100.9 (C-i"), 74.5 (C-4"), 71.4 (C-4'), 70.3 (C-2'),. 65.6 (C-3), 61.3 (3"-OCH 3 ), 59.3 (2"-OCH 3 ), 53.7 (20-OCH 3 ), 50.6 (20-OCH 3 ), 42.7 (C-8), 42.6 (C-4), 41.0 /3'-N(CH 3
)
2 /, 40.5 (C-2), 34.5 (C-19), 21.9, (C 21), 21.1, 21.0, 20.7 (3xCOCH 3 ), 12.7 (C-22), 8.3 (C-18). FAB (MH*) 959. Example 4 4'-Demycarosyl-2',4',4"-tri-O-acetyl-20-deoxo-20-dibenzylamino-8a-aza-8a homotylosin (4) Compound 2 (2.8 g, 2.66 mmol) was dissolved in dry methylene chloride (60 ml), triethyl amine (3.7 ml), 4-dimethylaminopyridine (0.07 g) and acetic anhydride (0.25 ml, 1.64 mmol) were added and then the reaction solution was left to stand for 26 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 1 to give a TLC homogeneous product (4) (2.7 g; 92.9 %). TLC: Rf (B) 0.55; Rf (C) 0.47. IR (KBr) cm-1 1747, 1651, 1632, 1538, 1453, 1372, 1233, 1170, 1051. 'H NMR (CDCl 3 ) 6 ppm 7.22 ~ 7.41 (phenyl), 7.10 (H-11), 5.70 (H-13), 5.65 (H-10), WO 00/77016 PCT/HROO/00018 12 4.91 (8a-NH) exchangeable with D 2 0, 4.86 (H-2'), 4.74 (H-4'), 4.66 (H-i"), 4.46 (H-4"), 4.15 (H-l'), 3.61 (2x20-N-CH 2 -phenyl), 3.53 (3"-OCH 3 ), 3.50 (2"-OCH 3 ), 2.32 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 2.06 (COCH 3 ), 2.00 (COCH 3 ), 1.72 (H-22), 1.12 (H-21), 0.78 (H-18). C NMR (CDCl 3 ) 6 ppm 173.3 (C-1), 170.1, 169.9, 169.5 (3xCOCH 3 ), 166.1 (9-CONH), 144.8 (C-11), 137.9 (C-13), 135.2 (C-12), 119.3 (C-10), 102.3 (C-1'), 101.0 (C-1"), 74.6 (C-4"), 71.4 (C-4'), 70.4 (C-2'), 66.0 (C-3), 61.5 (3"-OCH 3 ), 59.3 (2"-OCH 3 ), 52.2 (C-20), 42.9 (C-8), 42.4 (C-4), 41.0 /3'-N(CH 3
)
2 /, 38.7 (C-2), 29.4 (C-19), 21.8 (C-21), 21.1, 21.0, 20.7 (3xCOCH 3 ), 12.7 (C-22), 8.4 (C-18), 20-N(CH 2
C
6
H
5
)
2 , 139.8, 129.1, 128.0, 126.6, 57.9. FAB (MH*) 1094. Example 5 4'-Demycarosyl-2',4'-di-O-acetyl-4"-deoxy-4"-oxo-8a-aza-8a-homotylosin 20 dimethylacetal (5) A solution of pyridine trifluoroacetate (1.0 g, 5.24 mmol) in methylene chloride (10 ml) was added drop by drop at 15'C to a solution of the compound 1 (1.0 g, 1.09 mmol), 1-(3-dimethylamiinopropyl)-3-ethylcarbodiimide hydrochloride (1.0 g, 5.22 mmol) and dimethyl sulfoxide (1.0 ml, 14.10 mmol) in methylene chloride (20 ml). The reaction mixture was stirred for 3 hours at room temperature, then poured into water (150 ml) and after separating the organic layer, it was extracted two more times with methylene chloride. The combined organic extracts were washed with a saturated NaHCO 3 solution and water, dried (K2C0 3 ) and evaporated at reduced pressure to a dry residue. The obtained crude product (0.95 g) was purified by flash chromatography on a silica gel column using the solvent system B to give a TLC homogeneous product (5) (0.45 g). TLC: Rf (B) 0.52. IR (KBr) cm 1 1749, 1657, 1620, 1542, 1455, 1375, 1230, 1172, 1060.
WO 00/77016 PCT/HROO/00018 13 1H NMR (CDCl 3 ) 6 ppm 7.16 (H-11), 5.71 (H-10), 5.64 (H-13), 4.97 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.76 (H-4'), 4.60 (H-20), 4.63 (H-i"), 4.33 (H-l'), 4.17 (H-8), 3.98 (H-5"), 3.78 (H-3"), 3.58 (3"-OCH 3 ), 3.52 (2"-OCH 3 ), 3.36 (20-OCH 3 ), 3.35 (20-OCH 3 ), 3.30 (H-2"), 2.33 /3'-N(CH 3
)
2 /, 2.05 (COCH 3 ), 2.03 (COCH 3 ), 1.76 (H-22), 1.34 (H-6"), 1.17 (H-21). 13 C NMR (CDCl 3 ) 6 ppm 202.4 (C-4"), 173.1 (C-1), 169.9, 169.5 (2xCOCH 3 ), 166.1 (9-CONH), 144.6 (C-11), 137.6 (C-13), 135.3 (C-12), 119.5 (C-10), 103.6 (C-20), 103.0 (C-1"), 102.1 (C-l'), 85.3 (C-3"), 84.2 (C-2"), 73.3 (C-5"), 71.3 (C-4'), 70.3 (C-2'), 65.6 (C-3), 60.2 (3"-OCH 3 ), 59.1 (2"-OCH 3 ), 53.7 (20-OCH 3 ), 50.5 (20-OCH 3 ), 42.7 (C-8), 42.6 (C-4), 41.0 /3'-N(CH 3
)
2 /, 40.7 (C-2) 34.4 (C-19), 21.9 (C-21), 21.1, 21.0 (2xCOCH 3 ), 14.0 (C-6"), C-12.7 (C-22), 8.3 (C-18). FAB (MH*) 915. Example 6 4'-Demycarosyl-2',4'-di-O-acetyl-4"-deoxy-4"-oxo-20-deoxo-20-dibenzylamino 8a-aza-8a-homotylosin (6) A solution of pyridmie trifluoroacetate (0.6 g, 3.11 mmol) in methylene chloride (6 ml) was added drop by drop at 15'C to a solution of the compound 2 (0.6 g, 0.57 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.6 g, 3.14 mmol) and dimethyl sulfoxide (0.45 ml, 6.35 mmol) in methylene chloride (20 ml). The reaction mixture was stirred for 5 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 5. The obtained crude product (0.54 g) was purified by flash chromatography on a silica gel column using the solvent system B to give a TLC homogeneous product (6) (0.28 g). TLC: Rf (B) 0.48; Rf (C) 0.33. IR (KBr) cm-1 1747, 1651, 1633, 1548, 1454, 1372, 1231, 1058. H NMR (CDCl 3 ) 5 ppm 7.25 ~ 7.41 (phenyl), 7.12 (H-11), 5.70 (H-13), 5.65 (H-10), 4.94 (8a-NH) exchangeable with D 2 0, 4.82 (H-2'), 4.74 (H-4'), 4.65 (H-i"), WO 00/77016 PCT/HROO/00018 14 4.15 (H-l'), 3.98 (H-5"), 3.78 (H-3"), 3.62 (20-N-CH 2 -phenyl), 3.58 (20-CH 2 -phenyl), 3.55 (3"-OCH 3 ), 3.49 (2"-OCH 3 ), 2.32 /3'-N(CH 3
)
2 /, 2.06
(COCH
3 ), 2.00 (COCH 3 ), 1.74 (H-22), 1.36 (H-6"), 1.12 (H-21). "C NMR (CDCl 3 ) 6 ppm 202.4 (C-4"), 173.4 (C-1), 169.8, 169.3 (2xCOCH 3 ), 166.1 (9-CONH), 144.6 (C-11), 137.0 (C-13), 135.6 (C-12), 119.6 (C-10), 103.0 (C-i"), 102.2 (C-l'), 85.3 (C-3"), 84.8 (C-2"), 73.3 (C-5"), 71.4 (C-4'), 70.4 (C-2'), 65.9 (C-3), 60.3 (3"-OCH 3 ), 59.1 (2"-OCH 3 ), 52.2 (C-20), 42.9 (C-8), 42.4 (C-4), 40.9 /3'-N(CH 3
)
2 /, 38.7 (C-2), 29.4 (C-19), 21.8 (C-21), 21.1, 21.0 (2xCOCH 3 ), 14.0 (C-6"), 12.8 (C-22), 8.4 (C-18), 20-N(CH 2
C
6
H
5
)
2 139.6, 129.9, 128.0, 126.6, 57.8. FAB (MH-) 1050. Example 7 4'-Demycarosyl-2',4',4"-tri-O-acetyl-3-deoxy-3-oxo-8a-aza-8a-homotylosin 20 dimethylacetal (7) A solution of pyridmie trifluoroacetate (3.0 g, 15.72 mmol) in methylene chloride (30 ml) was added drop by drop at 15'C to a solution of the compound 3 (2.0 g, 2.09 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (3.0 g, 15.66 mmol) and dimethyl sulfoxide (2.9 ml, 40.89 mmol) in methylene chloride (50 ml). The reaction mixture was stirred for 3 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 5. The obtained crude product (1.95 g) was purified by flash chromatography on a silica gel column using the solvent system C to give a TLC homogeneous product (7) (1.3 g). TLC: Rf (C) 0.58. IR (KBr) cm 1 1749, 1655, 1618, 1546, 1454, 1374, 1233, 1171, 1052. 1H NMR (CDCl 3 ) 6 ppm 6.90 (H-11), 5.76 (H-10), 5.43 (H-13), 4.96 (8a-NH) exchangeable with D 2 0, 4.89 (H-2'), 4.79 (H-4'), 4.66 (H-i"), 4.40 (H-i'), 4.18 (H-8), 3.55, 3.32 (H-2), 3.52 (3"-OCH 3 ), 3.49 (2"-OCH 3 ), 3.30 WO 00/77016 PCT/HRO0/00018 15 (20-OCH 3 ), 3.29 (20-OCH 3 ), 2.34 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 2.06 (COCH 3 ), 2.03 (COCH 3 ), 1.75 (H-22), 1.10 (H-21), 1.07 (H-18). 1 3 C NMR (CDCl 3 ) 6 ppm 205.6 (C-3), 172.9 (C-1), 170.1, 169.8, 169.4 (3xCOCH 3 ), 166.1 (9-CONH), 144.1 (C-11), 138.0 (C-13), 134.9 (C-12), 119.6 (C-10), 103.7 (C-20), 102.1 (C-l'), 100.9 (C-i"), 74.5 (C-4"), 71.4 (C-4'), 70.3 (C-2'), 61.3 (3"-OCH 3 ), 59.3 (2"-OCH 3 ), 53.7 (20-OCH 3 ), 50.6 (20-OCH 3 ), 46.5 (C-2), 44.2 (C-4), 42.0 (C-8), 41.0 /3'-N(CH 3
)
2 /, 34.5 (C-19), 21.9, (C-21), 21.1, 21.0, 20.7 (3xCOCH 3 ), 17.6 (C-18), 12.7 (C-22). FAB (MH) 957. Example 8 4'-Demycarosyl-2 ',4',4"-tri--acetyl-3-deoxy-3-oxo-20-deoxo-20-dibenzylamino 8a-aza-8a-homotylosin (8) A solution of pyridmie trifluoroacetate (2.0 g, 10.36 mmol) in methylene chloride (10 ml) was added drop by drop at 15'C to a solution of the compound 4 (1.0 g, 1.09 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (2.04 g, 10.44 mmol) and dimethyl sulfoxide (1.6 ml, 22.56 mmol) in methylene chloride (20 ml). The reaction mixture was stirred for 6 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 5. The obtained crude product (0.96 g) was purified by flash chromatography on a silica gel column using the solvent system B to give a TLC homogeneous product (8) (0.62 g). TLC: Rf (B) 0.60. IR (KBr) cm' 1748, 1633, 1538, 1454, 1373, 1231, 1052. 'H NMR (CDCl 3 ) 6 ppm 7.22 ~ 7.40 (phenyl), 6.89 (H- 11), 5.66 (H-10), 5.49 (H-13), 4.96 (8a-NH) exchangeable with D 2 0, 4.81 (H-2'), 4.74 (H-4'), 4.66 (H-1"), 4.42 (H-4"), 4.15 (H-l'), 4.12 (H-8), 3.78, 3.38 (H-2), 3.51 (2x20-N-CH 2 phenyl, 3"-OCH 3 ), 3.48 (2"-OCH 3 ), 2.32 /3'-N(CH 3
)
2 /, 2.22 (H-4), 2.09
(COCH
3 ), 2.06 (COCH 3 ), 2.00 (COCH 3 ), 1.72 (H-22), 1.10 (H-21), 1.08 (H-18).
WO 00/77016 PCT/HROO/00018 16 "C NMR (CDCl 3 ) 6 ppm 206.7 (C-3), 172.7 (C-1), 170.1, 169.9, 169.5 (3xCOCH 3 ), 166.1 (9-CONH), 144.0 (C-11),. 136.5 (C-12), 135.0 (C-13), 119.9 (C-10), 102.7 (C-i'), 100.9 (C-i"), 74.6 (C-4"), 71.3 (C-4'), 70.3 (C-2'), 61.3 (3"-OCH 3 ), 59.3 (2"-OCH 3 ), 51.7 (C-20), 47.7 (C-2), 44.5 (C-4)), 42.0 (C-8), 41.0 /3'-N(CH 3
)
2 /, 28.6 (C-19), 22.0 (C-21), 21.0, 20.7 (3xCOCH 3 ), 17.8 (C-18), 13.1 (C-22), 20-N(CH 2
C
6
H
2 ), 140.1, 128.9, 128.0, 126.4, 57.9. FAB (MH-) 1092. Example 9 4'-Demycarosyl-4"-deoxy-4"-oxo-8a-aza-8a-homotylosin 20-dimethylacetal (9) The compound 5 (0.65 g, 0.71 mmol) was dissolved in methanol (20 ml) and left to stand at room temperature for 48 hours. To the reaction solution a saturated NaHCO 3 solution was added and it was extracted twice with chloroform. The combined organic extracts were dried (K 2 C0 3 ) and evaporated at reduced pressure to a dry residue. The obtained crude product (0.45 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (9) (0.20 g). TLC: Rf (A) 0.27. IR (KBr) cm' 1749, 1657, 1620, 1542, 1455, 1375, 1230, 1172, 1060. 1 H NMR (CDC1 3 ) 6 ppm 7.16 (H-11), 5.72 (H-10), 5.67 (H-13), 4.99 (8a-NH) exchangeable with D 2 0, 4.60 (H-20), 4.63 (H-i"), 4.33 (H-l'), 4.17 (H-8), 3.98 (H-5"), 3.78 (11-3"), 3.58 (3"-OCH 3 ), 3.52 (2"-OCH 3 ), 3.46 (H-2'), 3.36, 3.35 (2x20-OCH 3 ), 3.30 (H-2"), 3.06 (H-4'), 2.33 /3'-N(CH 3
)
2 /, 1.76 (H-22), 1.34 (H-6"), 1.17 (H-21). 1C NMR (CDCl 3 ) 6 ppm 202.4 (C-4"), 173.1 (C-1), 166.1 (9-CONH), 144.6 (C-11), 137.6 (C-13), 135.3 (C-12), 119.5 (C-10), 103.6 (C-20), 103.0 (C-1"), 102.1 (C-i'), 85.3 (C-3"), 84.2 (C-2"), 73.3 (C-5"), 65.6 (C-3), 60.2 (3"-OCH 3 ), 59.1 (2"-OCH 3 ), 53.7 (20-OCH 3 ), 50.5 (20-OCH 3 ), 42.7 (C-8), 42.6 (C-4), 41.0 WO 00/77016 PCT/HR0O/00018 17 /3'-N(CH 3
)
2 /, 40.7 (C-2), 34.4 (C-19), 21.9 (C-21), 14.0 (C-6"), 12.7 (C-22), 8.3 (C-18). FAB (MH) 831. Example 10 4'-Demycarosyl-4"-deoxy-4"-oxo-20-deoxo-20-dibenzylamino-8a-aza-8a homotylosin (10) The compound 6 (0.30 g, 0.73 mmol) was dissolved in methanol (20 ml) and left to stand at room temperature for 30 hours. After addition of water (50 ml) the product was isolated by a gradient extraction with chloroform at pH 4.5 and 7.5. The combined chloroform extracts at pH 7.5 were dried (K 2 C0 3 ) and evaporated at reduced pressure and the obtained product (0.17 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (10) (0.08 g). TLC: Rf (A) 0.49. IR (KBr) cm-1 1715, 1655, 1619, 1542, 1454, 1377, 1168, 1082. H NMR (CDCl 3 ) 6 ppm 7.25 ~ 7.41 (phenyl), 7.12 (H-11), 5.70 (H-13), 5.65 (H-10), 4.94 (8a-NH) exchangeable with D 2 0, 4.84 (H-2'), 4.74 (H-4'), 4.60 (H-i"), 4.15 (H-i'), 3.98 (H-5"), 3.78 (H-3"), 3.62 (3"-OCH 3 ), 3.61 (20-N-CH 2 phenyl), 3.58 (20-CH 2 -phenyl), 3.51 (2"-OCH 3 ), 3.46 (H-2'), 3.01 (H-4'), 2.32 /3'-N(CH 3
)
2 /, 1.72 (H-22), 1.12 (H-21). 13C NMR (CDCl 3 ) 6 ppm 202.4 (C-4"), 173.4 (C-1), 166.1 (9-CONH), 144.7 (C-11), 137.1 (C-13), 135.6 (C-12), 119.7 (C-10), 104.2 (C-i'), 103.0 (C-i"), 85.4 (C-3"), 84.9 (C-2"), 73.3 (C-5"), 66.4 (C-3), 59.8 (3"-OCH 3 ), 58.6 (2"-OCH 3 ), 52.2 (C-20), 43.3 (C-8), 42.3 (C-4), 41.5 /3'-N(CH 3
)
2 /, 38.7 (C-2), 29.4 (C-19), 22.0 (C-21), 14.1 (C-6"), 12.8 (C-22), 9.1 (C-18), 20-N(CH 2
C
6
H
5
)
2 139.8, 129.1, 128.0, 126.6, 58.0. FAB (MH*) 967.
WO 00/77016 PCT/HROO/00018 18 Example ]] 4'-Demycarosyl-4"-O-acetyl-3-deoxy-3-oxo-8a-aza-8a-homotylosin 20 dimethylacetal (11) The compound 7 (0.70 g, 0.73 mmol) was dissolved in methanol (50 ml) and left to stand at room temperature for 24 hours. The isolation of the product was carried out in the manner disclosed in Example 9 and the obtained crude product (0.62 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (11) (0.40 g). TLC: Rf (A) 0.44. IR (KBr) cm' 1749, 1657, 1620, 1544, 1455, 1375, 1229, 1170, 1063. 'H NMR (CDCl 3 ) 6 ppm 6.87 (H-11), 5.77 (H-10), 5.44 (H-13), 5.18 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.64 (H-i"), 4.44 (H-4"), 4.30 (H-l'), 4.17 (H-8), 3.93 (H-5"), 3.89 (H-3"), 3.53 (3"-OCH 3 ), 3.50, 3.26 (H-2), 3.48 (2"-OCH 3 ), 3.30 (20-OCH 3 ), 3.29 (20-OCH 3 ), 2.53 /3'-N(CH 3
)
2 /, 2.12
(COCH
3 ), 1.75 (H-22), 1.25 (H-18). 1 3 C NMR (CDCl 3 ) 6 ppm 205.4 (C-3), 172.9 (C-1), 170.1 (COCH 3 ), 167.4 (9 CONH), 143.4 (C-11), 136.2 (C-12), 134.6 (C-13), 120.7 (C-10), 104.2,(C-1'), 103.9 (C-20), 100.8 (C-i"), 74.5 (C-4"), 70.9 (C-2'), 70.5 (C-2'), 61.3 (3"
OCH
3 ), 59.0 (2"-OCH 3 ), 52.6 (20-OCH 3 ), 52.1 (20-OCH 3 ), 45.9 (C-2), 44.4 (C-4), 42.5 (C-8), 41.4 /3'-N(CH 3
)
2 /, 33.8 (C-19), 22.0 (C-21), 20.7 (COCH 3 ), 17.5 (C-18), 12.9 (C-22). FAB (MH-) 873. Example 12 4'-Demycarosyl-4"-O-acetyl-3-deoxy-3-oxo-20-deoxo-20-dibenzylamino-8a-aza 8a-homotylosin (12) WO 00/77016 PCT/HROO/00018 19 The compound 8 (1.20 g, 10.99 mmol) was dissolved in methanol (100 ml) and left to stand at room temperature for 24 hours. To the reaction solution water (100 ml) was added and it was extracted with methylene chloride at pH 6.5. The combined organic extracts were dried (K 2 C0 3 ) and evaporated at reduced pressure and the obtained crude product (1.0 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (12) (0.52 g). TLC: Rf (A) 0.65. IR (KBr) cm 1 1745, 1650, 1622, 1537, 1454, 1373, 1233, 1166, 1058. 'H NMR (CDCl 3 ) 6 ppm 7.25 ~ 7.41 (phenyl), 6.90 (H-11), 5.67 (H-10), 5.52 (H-13), 4.98 (8a-NH) exchangeable with D 2 0, 4.67 (H-i"), 4.45 (H-4"), 4.17 (H-i'), 4.02 (H-8), 3.61 (20-CH 2 -phenyl), 3.53 (3"-OCH 3 ), 3.52 (20-CH 2 -phenyl), 3.50 (2"-OCH 3 ), 3.76, 3.32 (H-2), 2.52 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 1.73 (H 22), 1.21 (H-18), 1.08 (H-21). 13C NMR (CDCl 3 ) 6 ppm 205.3 (C-3), 172.5 (C-1), 170.1 (COCH 3 ), 167.2 (9 CONH), 143.9 (C-11), 135.9 (C-12), 135.4 (C-13), 120.0 (C-10), 103.9 (C-I'), 100.9 (C-i"), 74.6 (C-4"), 70.7 (C-4'), 70.4 (C-2'), 61.3 (3"-OCH 3 ), 59.3 (2"
OCH
3 ), 51.6 (C-20), 46.1 (C-2), 44.5 (C-4), 43.3 (C-8), 41.5 /3'-N(CH 3
)
2 /, 28.8 (C-19), 22.0 (C-21), 20.7 (COCH 3 ), 17.8 (C-18), 12.9 (C-22), 20
N(CH
2
C
6
H)
2 139.9, 128.8, 128.0, 126.5, 58.0. FAB (MH*) 1008. Example 13 4'-Demycarosyl-4"-O-acetyl-8a-aza-8a-homotylosin 20-dimethylacetal (13) The compound 3 (0.5 g, 0.52 mmol) was dissolved in methanol (20 ml) and left to stand at room temperature for 24 hours. The isolation of the product was carried out in the manner disclosed in Example 9 and the obtained crude product (0.43 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (13) (0.32 g).
WO 00/77016 PCT/HROO/00018 20 TLC: Rf (A) 0.32. IR (KBr) cm- 1739, 1656, 1616, 1541, 1455, 1376, 1237, 1170, 1062. 1H NMR (CDCl 3 ) 6 ppm 7.15 (H-11), 5.71 (H-10), 5.66 (H-13), 4.97 (8a-NH) exchangeable with D 2 0, 4.64 (H-1"), 4.62 (H-20), 4.44 (H-4"), 4.24 (H-l'), 4.18 (H-8), 3.53 (3"-OCH 3 ), 3.47 (2"-OCH 3 ), 3.37 (20-OCH 3 ), 3.36 (20
OCH
3 ), 2.50 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 1.75 (H-22), 1.17 (H-21). FAB (MH) 875. Example 14 4'-Demycarosyl-4"-O-acetyl-20-deoxo-20-dibenzylamino-8a-aza-8a-homotylosin (14) The compound 4 (0.75 g, 0.69 mmol) was dissolved in methanol (20 ml) and left to stand at room temperature for 24 hours. The isolation of the product was carried out in the manner disclosed in Example 12 and the obtained crude product (0.66 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (14) (0.45 g). TLC: Rf (A) 0.50. IR (KBr) cm 1740, 1657, 1621, 1538, 1454, 1373, 1236, 1169, 1054. 'H NMR (CDCl 3 ) 6 ppm 7.25 ~ 7.41 (phenyl), 7.10 (H-11), 5.69 (H-13), 5.65 (H-10), 4.96 (8a-NH) exchangeable with D 2 0, 4.66 (H-i"), 4.45 (H-4"), 4.14 (H-8), 4.07 (H-l'), 3.59 (20-N-CH 2 -phenyl), 3.56 (20-CH 2 -phenyl), 3.53 (3"-OCH 3 ), 3.50 (2"-OCH 3 ), 2.49 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 1.73 (H-22), 1.11 (H-21), 0.94 (H-18). FAB (MH) 1010. Example 15 4'-Demycarosyl-3-deoxy-3-oxo-8a-aza-8a-homotylosin 20-dimethylacetal (15) WO 00/77016 PCT/HROO/00018 21 The compound 11 (0.40 g, 0.46 mmol) was dissolved in a methanol/conc. NH 4 0H mixture (4:1, 50 ml) and left to stand for 60 hours at the temperature of 5'C. The reaction solution was evaporated to an oily residue and then a product was isolated in the manner disclosed in Example 9. The obtained crude product (0.25 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (15) (0.15 g). TLC: Rf (A) 0.39. IR (KBr) cm-1 1739, 1714, 1650, 1620, 1544, 1455, 1375, 1170, 1063. 'H NMR (CDCl 3 ) 6 ppm 6.87 (H-11), 5.77 (H-10), 5.44 -(H-13), 5.18 (8a-NH) exchangeable with D 2 0, 4.60 (H-20), 4.64 (H-1"), 4.33 (H-l'), 4.17 (H-8), 3.93 (H-5"), 3.89 (H-3"), 3.53 (3"-OCH 3 ), 3.50, 3.26 (H-2), 3.48 (2"-OCH 3 ), 3.30 (20-OCH 3 ), 3.29 (20-OCH 3 ), 2.33 /3'-N(CH 3
)
2 /, 1.75 (H-22), 1.25 (H-18). FAB (MH-) 831. Example 16 4'-Demycarosyl-3-deoxy-3-oxo-20-deoxo-20-dibenzylamino-8a-aza-8a homotylosin (16) The compound 12 (0.78 g, 0.77 mmol) was dissolved in a methanol/cone. NH 4 0H mixture (4:1, 50 ml) and left to stand for 24 hours at room temperature. To the reaction solution water (80 ml) was added and it was extracted twice with methylene chloride at pH 7.5. The combined organic extracts were dried (K 2 C0 3 ) and evaporated at reduced pressure and the obtained product (0.66 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (16) (0.32 g). TLC: Rf (A) 0.55. IR (KBr) cm-1 1739, 1714, 1650, 1622, 1538, 1454, 1376, 1167, 1082. 'H NMR (CDCl 3 ) 6 ppm 7.25 ~ 7.41 (phenyl), 6.90 (H-11), 5.66 (H-13), 5.53 (H-10), 5.28 (8a-NH) exchangeable with D 2 0, 4.61 (H-1"), 4.16 (H-l'), 4.03 (H-8), WO 00/77016 PCT/HROO/00018 22 3.62 (20-N-CH 2 -phenyl), 3.61 (20-CH 2 -phenyl, 3"-OCH 3 ), 3.51 (2"-OCH 3 ), 3.78, 3.38 (H-2), 2.5 /3'-N(CH 3
)
2 /, 2.38 (H-4), 1.72 (H-22), 1.21 (H-18), 1.08 (H-21). 1 3 C NMR (CDCl 3 ) 6 ppm 205.3 (C-3), 172.5 (C-1), 167.2 (9-CONH), 143.9 (C-11), 135.9 (C-12), 135.6 (C-13), 120.0 (C-10), 103.9 (C-l'), 101.0 (C-l'), 72.5 (C-4"), 70.7 (C-4'), 70.4 (C-2'), 61.5 (3"-OCH 3 ), 59.5 (2"-OCH 3 ), 51.7 (C-20), 46.1 (C-2), 44.5 (C-4), 43.3 (C-8), 41.5 /3'-N(CH 3
)
2 /, 28.8 (C-19), 22.0 (C-21), 17.8 (C-18), 12.9 (C-22), 20-N(CH 2
C
6
H)
2 140.0, 128.8, 128.0, 126.5, 58.0. FAB (MH-) 967. Example 17 4'-Demycarosyl-3-deoxy-3-oxo-8a-aza-8a-homotylosin (17) The compound 15 (0.5 g, 0.60 mmol) was dissolved in an acetonitrile/0.1 N HCi mixture (1:1, 35 ml) and stirred for 2 hours at room temperature. To the reaction solution a saturated NaHC0 3 solution was added and it was extracted twice with methylene chloride. The combined organic extracts were dried (K 2 C0 3 ) and evaporated at reduced pressure and the obtained product (0.42 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (17) (0.25 g). TLC: Rf (A) 0.35. IR (KBr) cm' 1739, 1719, 1657, 1620, 1545, 1455, 1376, 1169, 1082. 1H NMR (CDC 3 ) 6 ppm 9.78 (H-20), 7.19 (H-11), 5.72 (H-10), 5.70 (H-13), 5.06 (8a-NH) exchangeable with D 2 0, 4.58 (H-i"), 4.18 (H-l'), 4.23 (H-8), 3.68, 3.32 (H-2), 3.62 (3"-OCH 3 ), 3.49 (2"-OCH 3 ), 2.49 /3'-N(CH 3
)
2 /, 1.75 (H-22), 1.25 (H-18), 1.18 (H-21). 13C NMR (CDCl 3 ) 6 ppm 205.3 (C-3), 203.8 (C-20), 173.5 (C-1), 166.9 (9-CONH), 145.1 (C-11), 138.2 (C-13), 135.1 (C-12), 129.3 (C-10), 103.7 (C-l'), 101.1 (C-i'), 72.8 (C-4"), 71.0 (C-4'), 70.4 (C-2'), 61.5 (3"-OCH 3 ), 59.5 (2"-OCH 3
),
WO 00/77016 PCT/HROO/00018 23 46.6 (C-19), 46.1 (C-2), 44.5 (C-4), 43.3 (C-8), 41.5 /3'-N(CH 3
)
2 /, 22.4 (C-21), 17.8 (C-18), 12.9 (C-22). FAB (MH) 785. Example 18 4'-Demycarosyl-2',4'-di-O-acetyl-8a-aza-8a-homotylosin (18) The compound 1 (0.5 g, 0.55 mmol) was dissolved in an acetonitrile/0.1 N HCI mixture (1:1, 35 ml) and stirred for 2 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 17 to give a TLC homogeneous product (18) (0.34 g). TLC: Rf (B) 0.35. IR (KBr) cm' 1749, 1657, 1620, 1548, 1455, 1375, 1231, 1170, 1059. 'H NMR (CDCl 3 ) 5 ppm 9.75 (H-20), 7.21 (H-11), 5.72 (H-10), 5.71 (H-13), 5.08 (8a-NH) exchangeable with D 2 0, 4.89 (H-2'), 4.74 (H-4'), 4.58 (H-1"), 4.26 (H-i'), 3.61 (3"-OCH 3 ), 3.49 (2"-OCH 3 ), 2.33 /3'-N(CH 3
)
2 /, 2.05 (COCH 3 ), 2.03 (COCH 3 ), 1.74 (H-22), 1.18 (H-21). 13 C NMR (CDCl 3 ) 6 ppm 203.6 (C-20), 173.3 (C-1), 169.9, 169.5 (2xCOCH 3 ), 166.5 (9-CONH), 145.2 (C-11), 138.3 (C-13), 135.0 (C-12), 119.0 (C-10), 101.6 (C-1'), 100.9 (C-1"), 72.5 (C-4"), 70.6 (C-4'), 70.3 (C-2'), 65.6 (C-3), 61.5 (3"-OCH 3 ), 59.5 (2"-OCH 3 ), 46.3 (C-19), 42.5 (C-8), 41.0 /3'-N(CH 3
)
2 /, 38.5 (C-2), 21.6 (C-21), 21.1, 21.0 (2xCOCH 3 ), 12.7 (C-22), 8.1 (C-18). FAB (MH-) 871. Example 19 4'-Demycarosyl-2',4',4"-tri-O-acetyl-8a-aza-8a-homotylosin (19) The compound 3 (0.5 g, 0.52 mmol) was dissolved in an acetonitrile/0.1 N HCl mixture (1:1, 35 ml) and stirred for 2 hours at room temperature. The isolation of the WO 00/77016 PCT/HROO/00018 24 product was carried out in the manner disclosed in Example 17 to give a TLC homogeneous product (19) (0.47 g). TLC: Rf (B) 0.60; Rf (C) 0.50. IR (KBr) cm 4 1748, 1659, 1621, 1538, 1455, 1373, 1232, 1171, 1052. H NMR (CDCl 3 ) 8 ppm 9.74 (H-20), 7.16 (H-11), 5.69 (H-10), 5.65 (H-13), 4.89 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.76 (H-4'), 4.64 (H-i"), 4.44 (H-4"), 4.33 (H-l'), 4.18 (H-8), 3.52 (3"-OCH 3 ), 3.46 (2"-OCH 3 ), 2.33 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 2.05 (COCH 3 ), 2.03 (COCH 3 ), 1.74 (H-22), 1.16 (H-21). 13 C NMR (CDCl 3 ) 6 ppm 203.6 (C-20), 173.1 (C-1), 170.1, 169.8, 169.4 (3xCOCH 3 ), 166.1 (9-CONH), 144.7 (C-11), 138.0 (C-13), 134.9 (C-12), 119.2 (C-10), 103.7 (C-20), 102.1 (C-l'), 100.9 (C-1"), 74.5 (C-4"), 71.4 (C-4'), 70.3 (C-2'), 65.6 (C-3), 61.3 (3"-OCH 3 ), 59.3 (2"-OCH 3 ), 46.3 (C-19), 42.7 (C-8), 42.6 (C-4), 41.0 /3'-N(CH 3
)
2 /, 40.5 (C-2), 34.5 (C-19), 21.9 (C-21), 21.1, 21.0, 20.7 (3xCOCH 3 ), 12.7 (C-22), 8.3 (C-18). FAB (MH) 913. Example 20 4'-Demycarosyl-2',4'-di-O-acetyl-4"-deoxy-4"-oxo-8a-aza-8a-homotylosin (20) The compound 5 (0.7 g, 0.77 mmol) was dissolved in an acetonitrile/0.1 N HCl mixture (1:1, 50 ml) and stirred for 1 hour at room temperature. The isolation of the product was carried out in the manner disclosed in Example 17 to give a TLC homogeneous product (20) (0.36 g). TLC: Rf (B) 0.48. IR (KBr) cm 1 1749, 1656, 1619, 1543, 1458, 1375, 1230, 1172, 1058. H NMR (CDCl 3 ) 5 ppm 9.75 (H-20), 7.21 (H-11), 5.72 (H-10), 5.70 (H-13), 5.08 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.74 (H-4'), 4.58 (H-i"), 4.30 (H-l'), 4.17 (H-8), 3.98 (H-5"), 3.78 (H-3"), 3.58 (3"-OCH 3 ), 3.48 (2"-OCH 3
),
WO 00/77016 PCT/HRO/00018 25 3.30 (H-2"), 2.33 /3'-N(CH 3
)
2 /, 2.05 (COCH 3 ), 2.03 (COCH 3 ), 1.76 (H-22), 1.34 (H-6"), 1.17 (H-21). "C NMR (CDC1 3 ) 6 ppm 203.0 (C-20), 202.4 (C-4"), 173.1 (C-1), 169.9, 169.5 (2xCOCH 3 ), 166.5 (9-CONH), 145.0 (C-11), 138.1 (C-13), 135.1 (C-12), 119.0 (C-10), 102.1 (C-i"), 100.9 (C-i'), 85.3 (C-3"), 84.2 (C-2"), 73.3 (C-5"), 71.3 (C-4'), 70.3 (C-2'), 65.6 (C-3), 61.5 (3"-OCH 3 ), 59.4 (2"-OCH 3 ), 46.3 (C-19), 42.5 (C-8), 41.0 /3'-N(CH 3
)
2 /, 38.5 (C-2), 21.9 (C-21), 21.1, 21.0 (2xCOCH 3 ), 14.0 (C-6"), 12.7 (C-22), 8.3 (C-1). FAB (MH*) 869. Example 21 4'-Demycarosyl-4"-O-acetyl-8a-aza-8a-homotylosin (21) The compound 19 (0.30 g, 0.33 mmol) was dissolved in methanol (20 ml) and left to stand for 24 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 9 and the obtained crude product (0.25 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (21) (0.19 g). TLC: Rf (A) 0.28. IR (KBr) cm~1 1749, 1657, 1620, 1544, 1455, 1375, 1229, 1170, 1063. 1H NMR (CDC 3 ) 6 ppm 9.78 (H-20), 7.20 (H-11), 5.72 (H-10), 5.70 (H-13), 5.12 (8a-NH) exchangeable with D 2 0, 4.88 (H-2'), 4.64 (H-I"), 4.44 (H-4"), 4.18 (H-1'), 4.12 (H-8), 3.93 (H-5"), 3.89 (H-3"), 3.53 (3"-OCH 3 ), 3.48 (2"-OCH 3 ), 2.49 /3'-N(CH 3
)
2 /, 2.12 (COCH 3 ), 1.75 (H-22). FAB (MH) 829. Example 22 4'-Demycarosyl-4"-deoxy-4"-oxo-8a-aza-8a-homotylosin (22) WO 00/77016 PCT/HROO/00018 26 The compound 20 (0.23 g, 0.27 mmol) was dissolved in methanol (20 ml) and left to stand for 24 hours at room temperature. The isolation of the product was carried out in the manner disclosed in Example 9 and the obtained crude product (0.14 g) was purified by flash chromatography on a silica gel column using the solvent system A to give a TLC homogeneous product (22) (0.095 g). TLC: Rf (A) 0.30. IR (KBr) cm-1 1717, 1655, 1625, 1542, 1454, 1378, 1170, 1062. H NMR (CDCl 3 ) 6 ppm 9.76 (H-20), 7.20 (H-11), 5.72 (H-10), 5.70 (H-13), 5.12 (8a-NH) exchangeable with D 2 0, 4.64 (H-i"), 4.33 (H-i'), 4.18 (H-8), 3.98 (H-5"), 3.78 (H-3"), 3.58 (3"-OCH 3 ), 3.46 (2"-OCH 3 ), 3.30 (H-2"), 3.06 (H-4'), 2.33 /3'-N(CH 3
)
2 /, 1.74 (H-22), 1.34 (H-6"), 1.16 (H-21). 3 C NMR (CDC1 3 ) 6 ppm 203.7 (C-20), 202.5 (C-4"), 173.4 (C-1), 166.6 (9-CONH), 144.9 (C-11), 137.6 (C-13), 135.4 (C-12), 119.4 (C-10), 102.1 (C-l'), 100.9 (C-i"), 71.4 (C-4'), 70.3 (C-2'), 66.3 (C-3), 61.5 (3"-OCH 3 ), 59.7 (2"-OCH 3 ), 46.2 (C-19), 42.7 (C-8), 42.1 (C-4), 41.5 /3'-N(CH 3
)
2 /, 39.8 (C-2), 21.7 (C-21), 14.0 (C-6"), 12.7 (C-22), 8.7 (C-18). FAB (MH) 785.
Claims (24)
1. Compounds of the general formula I CO-NH CH3 8a R 20 H 3C R 2 H 3 C N(CH 3 ) 2 O H 3 C R10 OR1 O0H 3 30 OCH3 R5 CH 3 I wherein R represents CHO, CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R represents H or C 1 -C 3 acyl, R2 represents OR and R 6 represents H or C 1 -C 3 acyl, R 3 represents H or R 2 and R 3 together represent =0, R represents OH, R 5 represents H or R 4 and R 5 together represent =0.
2. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R' represents COCH 3 , R2 represents OR wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH.
3. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , RI represents COCH 3 , R 2 represents OR 6 wherein R 6 represents H, R 3 and R 5 are the same and represent H and R represents OH.
4. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R' represents COCH 3 , R 2 represents OR 6 wherein R 6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH. WO 00/77016 PCT/HROO/00018 28
5. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , R' represents COCH 3 , R represents OR wherein R 6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH.
6. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R' represents COCH 3 , R 2 and R 3 together represent =0, R 4 represents OH and R 5 represents H.
7. A compound according to claim 1, characterized .in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , RI represents COCH 3 , R 2 and R 3 together represent =0, R 4 represents OH and R 5 represents H.
8. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R 1 represents COCH 3 , R2 represents OR wherein R 6 represents COCH 3 , R 3 represents H and R 4 and R 5 together represent =0.
9. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , RI represents COCH 3 , R 2 represents OR 6 wherein R 6 represents COCH 3 , R 3 represents H and R 4 and R 5 together represent =0.
10. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R 1 and R 5 are the same and represent H, R 2 and R 3 together represent =0 and R 4 represents OH.
11. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , R' and R 5 are the same and represent H, R2 and R 3 together represent =0 and R 4 represents OH.
12. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R' and R 3 are the same and represent H, R 2 represents OR 6 wherein R 6 represents COCH 3 , and R 4 and R 5 together represent =0. WO 00/77016 PCT/HROO/00018 29
13. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , R1 and R 3 are the same and represent H, R 2 represents OR 6 wherein R 6 represents COCH 3 , and R 4 and R 5 together represent =0.
14. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R', R3 and R are the same and represent H, R2 represents OR 6 wherein R6 represents COCH 3 , and R 4 represents OH.
15. A compound according to claim 1, characterized .in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , R', R 3 and R 5 are the same and represent H, R 2 represents OR 6 wherein R represents COCH 3 , and R 4 represents OH.
16. A compound according to claim 1, characterized in that R represents CH(OCH 3 ) 2 , R 1 and R 3 are the same and represent H, R 2 represents OR 6 wherein R 6 represents H, and R 4 and R 5 together represent =0.
17. A compound according to claim 1, characterized in that R represents CH 2 N[CH 2 (C 6 H 5 )] 2 , RI and R 3 are the same and represent H, R 2 represents OR 6 wherein R represents H, and R 4 and R 5 together represent =0.
18. A compound according to claim 1, characterized in that R represents CHO, R 1 and R3 are the same and represent H, K represents OR6 wherein R6 represents H, and R 4 and R 5 together represent =0.
19. A compound according to claim 1, characterized in that R represents CHO, R represents COCH 3 , R 2 represents OR 6 wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH.
20. A compound according to claim 1, characterized in that R represents CHO, R represents COCH 3 , K 2 represents OR 6 wherein R 6 represents COCH 3 , R 3 and R 5 are the same and represent H and R 4 represents OH. WO 00/77016 PCT/HROO/00018 30
21. A compound according to claim 1, characterized in that R represents CHO, R' represents COCH 3 , R 2 and R 3 together represent =O, R 4 represents OH and R 5 represents H.
22. A compound according to claim 1, characterized in that R represents CHO, R', R3 and R are the same and represent H, R2 represents OR 6 wherein R6 represents COCH 3 , and R 4 represents OH.
23. A compound according to claim 1, characterized in that R represents CHO, R' and R' are the same and represent H, R2 and R3 together represent =0 and R 4 represents OH.
24. Process for the preparation of the compounds of the general formula I CO-NH CH3 8a R 20 H 3C R 2 H 3 C H 3 R N(CH 3 ) 2 3 H3 R 4'3'OR 1 4o 0 OT H 3 OCH 3 R5 CH 3 wherein R represents CHO, CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , RI represents H or C 1 -C 3 acyl, R represents OR and R6 represents H or C 1 -C 3 acyl, R 3 represents H or R2 and R 3 together represent =0, R represents OH, R5 represents H or R 4 and R 5 together represent =0, characterized in that WO 00/77016 PCT/HROO/00018 31 4'-demycarosyl-8a-aza-8a-homotylosin 20-dimethylacetal of the formula Ila and 4' demycarosyl-20-deoxo-20-dibenzylamino-8 a-aza-8a-homotylosin of the formula IIb CO-NH CH3 8a R 20 H 3 0 H 3 C H 3 N(OH 3 ) 2 H HOC 3 H OCH 3 OCH 3 OH CH 3 Ila R = CH(OCH 3 ) 2 Ilb R = CH 2 N[CH 2 (C 6 H 5 )] 2 are subjected to A) an O-acylation with anhydrides of C 1 -C 3 carboxylic acids, preferably with acetic acid anhydride in methylene chloride during 15 minutes to 1 hour at room temperature, and the obtained compounds of the formula I, wherein R represents 1 2 6 CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R represents COCH 3 , R 2 represents OR , wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH, are optionally subjected to Al) an O-acylation with anhydrides of C 1 -C 3 carboxylic acids, preferably with acetic acid anhydride in methylene chloride in the presence of an organic base, preferably triethyl amine and 4-dimethylaminopyridine as a catalyst during 30 hours at room temperature, and the obtained compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R' represents COCH 3 , R2 represents OR 6 , wherein R 6 represents COCH 3 , R3 and R 5 are the same and represent H and R 4 represents OH, WO 00/77016 PCT/HRO0/00018 32 are optionally subjected to B) an oxidation reaction with N(3-dimethylaniino-propyl)-N'ethyl carbodiimide hydrochloride in the presence of dimethylsulfoxide and pyridine trifluoroacetate as a catalyst in an inert solvent, preferably methylene chloride, during 2 to 6 hours at a temperature from 10'C to room temperature, and the obtained compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , RI represents COCH 3 , R2 represents OR6, wherein R 6 represents COCH 3 , R3 represents H and R 4 and R 5 together represent =0, are optionally subjected to C) methanolysis at room temperature for 2 days and the obtained compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents COCH 3 , and R 4 and R 5 together represent =O, are optionally subjected to C1) an alkaline methanolysis in a mixture of methanol and 25% ammonia (4:1) at a temperature from 5'C to room temperature during 20 to 60 hours to obtain compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and R 5 together represent =0; or the compound obtained according to process C1 of the formula I, wherein R represents CH(OCH 3 ) 2 , RI and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and R 5 together represent =0, is optionally subjected to D) a hydrolysis of the acetal in a mixture of acetonitrile and 0. 1 N hydrochloric acid (1:1) for 2 hours at room temperature to obtain the compound of the formula I, wherein R represents a CHO group, R' and R 3 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents H, and R 4 and R 5 together represent =O; WO 00/77016 PCT/HROO/00018 33 or compounds obtained according to process A of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R' represents COCH 3 , R 2 represents OR 6 , wherein R 6 represents H, R 3 and R 5 are the same and represent H and R 4 represents OH, are optionally subjected to oxidation in the manner disclosed in B, and the obtained compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R' represents COCH 3 , R2 and R 3 together represent =0, R 4 represents OH and R 5 represents H, are optionally subjected to methanolysis in the manner disclosed in C, to obtain compounds of the formula I, wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R 1 and R 5 are the same and represent H, R 2 and R 3 together represent =0 and R 4 represents OH; or the compound obtained according to process B of the formula I, wherein R represents a CH(OCH 3 ) 2 group, R' represents COCH 3 , R 2 and R 3 together represent =0, R4 represents OH and R 5 represents H, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, and the obtained compound of the formula I, wherein R represents a CHO group, R' represents COCH 3 , R 2 and R 3 together represent =O, R 4 represents OH and R 5 represents H, is optionally subjected to methanolysis in the manner disclosed in C, to obtain the compound of the formula I, wherein R represents a CHO group, R' and R are the same and represent H, R 2 and R 3 together represent =0 and R 4 represents OH; or the compound obtained according to process A WO 00/77016 PCT/HROO/00018 34 of the formula I, wherein R represents CH(OCH 3 ) 2 , R. represents COCH 3 , R2 represents OR, wherein Re represents H, R 3 and R' are the same and represent H and R 4 represents OH, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, to obtain a compound of the formula I wherein R represents CHO, R' represents COCH 3 , R2 represents OR 6 , wherein R 6 represents H, IR? and R5 are the same and represent H and R 4 represents OH; or compounds obtained according to process Al of the formula L wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 Hs)] 2 , R' represents COCH 3 , R represents OR 6 , wherein R6 represents COCH 3 , R3 and R5 are the same and represent H and R 4 represents OH, are optionally subjected to methanolysis in the manner disclosed in C, to obtain compounds of the formula L wherein R represents CH(OCH 3 ) 2 or CH 2 N[CH 2 (C 6 H 5 )] 2 , R', R3 and R5 are the same and represent H, R 2 represents OR 6 , wherein Ri represents COCH 3 , and RW represents OH; or the compound obtained according to process Al of the formula I, wherein R represents CH(OCH 3 ) 2 , R' represents COCH 3 , R2 represents OR 6 , wherein Ri represents COCH 3 , R? and R 5 are the same and represent H and R4 represents OH, is optionally subjected to a hydrolysis of acetal in the manner disclosed in D, and the obtained compound of the formula , wherein R represents CHO, R' represents COCH 3 , R2 represents OR, wherein R represents COCH 3 , R' and Rs are the same and represent H and R 4 represents OH, is optionally subjected to methanolysis in the manner disclosed in C, WO 00/77016 PCT/HROO/00018 35 to obtain the compound of the formula I, wherein R represents CHO, R', R 3 and R 5 are the same and represent H, R 2 represents OR 6 , wherein R 6 represents COCH 3 , and R 4 represents OH.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HR990192A HRP990192A2 (en) | 1999-06-11 | 1999-06-11 | 4'-DEMICAROZYL-8a-AZA-8a-HOMOTHILOSINE DERIVATIVES |
HRP990192A | 1999-06-11 | ||
PCT/HR2000/000018 WO2000077016A1 (en) | 1999-06-11 | 2000-06-06 | DERIVATIVES OF 4'-DEMYCAROSYL-8a-AZA-8a-HOMOTYLOSIN |
Publications (2)
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AU5558300A true AU5558300A (en) | 2001-01-02 |
AU767543B2 AU767543B2 (en) | 2003-11-13 |
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AU55583/00A Ceased AU767543B2 (en) | 1999-06-11 | 2000-06-06 | Derivatives of 4'-demycarosyl-8a-aza-8a-homotylosin |
Country Status (13)
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EP (1) | EP1189914A1 (en) |
JP (1) | JP2003502338A (en) |
AU (1) | AU767543B2 (en) |
CA (1) | CA2375812A1 (en) |
CZ (1) | CZ20014362A3 (en) |
EA (1) | EA200200026A1 (en) |
HR (1) | HRP990192A2 (en) |
HU (1) | HUP0201610A3 (en) |
NO (1) | NO322424B1 (en) |
SK (1) | SK17572001A3 (en) |
UA (1) | UA66930C2 (en) |
WO (1) | WO2000077016A1 (en) |
YU (1) | YU87401A (en) |
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US7091196B2 (en) | 2002-09-26 | 2006-08-15 | Rib-X Pharmaceuticals, Inc. | Bifunctional heterocyclic compounds and methods of making and using same |
KR20060002800A (en) * | 2003-03-05 | 2006-01-09 | 립-엑스 파마슈티칼즈, 인크. | Bifunctional heterocyclic compounds and methods of making and using the same |
JPWO2005019238A1 (en) | 2003-08-22 | 2006-10-19 | 明治製菓株式会社 | Novel azalides and azalactam derivatives and their production |
EP2716647A3 (en) | 2004-02-27 | 2014-08-20 | Rib-X Pharmaceuticals, Inc. | Macrocyclic compounds and methods of making and using the same |
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SI8710674B (en) * | 1987-04-14 | 1998-06-30 | Pliva | Process for preparation of 10,11,12,13-tetrahydro derivatives of tylosin |
YU149889A (en) * | 1989-07-26 | 1991-02-28 | Pliva Zagreb | Process for preparing biologically active derivatives of tylozine |
CZ211798A3 (en) * | 1997-07-16 | 1999-02-17 | Pliva, Farmaceutska, Kemijska, Prehrambena I Kozmetička Industrije, Dioničko Društvo | Linear 8a-secoazalides and process for preparing thereof |
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1999
- 1999-06-11 HR HR990192A patent/HRP990192A2/en not_active Application Discontinuation
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- 2000-06-06 HU HU0201610A patent/HUP0201610A3/en unknown
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- 2000-06-06 WO PCT/HR2000/000018 patent/WO2000077016A1/en not_active Application Discontinuation
- 2000-06-06 EP EP00940676A patent/EP1189914A1/en not_active Withdrawn
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NO322424B1 (en) | 2006-10-02 |
JP2003502338A (en) | 2003-01-21 |
SK17572001A3 (en) | 2002-04-04 |
HRP990192A2 (en) | 2001-04-30 |
HUP0201610A3 (en) | 2003-03-28 |
EP1189914A1 (en) | 2002-03-27 |
CA2375812A1 (en) | 2000-12-21 |
YU87401A (en) | 2004-07-15 |
UA66930C2 (en) | 2004-06-15 |
WO2000077016A1 (en) | 2000-12-21 |
HUP0201610A2 (en) | 2002-10-28 |
EA200200026A1 (en) | 2002-06-27 |
NO20016030L (en) | 2002-01-30 |
NO20016030D0 (en) | 2001-12-10 |
CZ20014362A3 (en) | 2002-04-17 |
AU767543B2 (en) | 2003-11-13 |
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