AU5454801A - Methods of enhancing splp-mediated transfection using endosomal membrane destabilizers - Google Patents

Methods of enhancing splp-mediated transfection using endosomal membrane destabilizers

Info

Publication number
AU5454801A
AU5454801A AU5454801A AU5454801A AU5454801A AU 5454801 A AU5454801 A AU 5454801A AU 5454801 A AU5454801 A AU 5454801A AU 5454801 A AU5454801 A AU 5454801A AU 5454801 A AU5454801 A AU 5454801A
Authority
AU
Australia
Prior art keywords
splp
enhancing
methods
mediated transfection
endosomal membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU5454801A
Other languages
English (en)
Inventor
Angela M I Lam
Lorne R Palmer
Pieter R Cullis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
Original Assignee
University of British Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/553,639 external-priority patent/US6852334B1/en
Priority claimed from PCT/CA2000/000451 external-priority patent/WO2000062813A2/en
Application filed by University of British Columbia filed Critical University of British Columbia
Publication of AU5454801A publication Critical patent/AU5454801A/xx
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Epidemiology (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU5454801A 2000-04-20 2001-04-20 Methods of enhancing splp-mediated transfection using endosomal membrane destabilizers Pending AU5454801A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/553,639 US6852334B1 (en) 1999-04-20 2000-04-20 Cationic peg-lipids and methods of use
PCT/CA2000/000451 WO2000062813A2 (en) 1999-04-20 2000-04-20 Cationic peg-lipids and methods of use
US22794900P 2000-08-25 2000-08-25
PCT/CA2001/000555 WO2001080900A2 (en) 2000-04-20 2001-04-20 Enhanced stabilised plasmid-lipid particle-mediated transfection using endosomal membrane

Publications (1)

Publication Number Publication Date
AU5454801A true AU5454801A (en) 2001-11-07

Family

ID=27168648

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2001254548A Ceased AU2001254548B2 (en) 2000-04-20 2001-04-20 Enhanced stabilised plasmid-lipid particle-mediated transfection using endosomal membrane
AU5454801A Pending AU5454801A (en) 2000-04-20 2001-04-20 Methods of enhancing splp-mediated transfection using endosomal membrane destabilizers

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AU2001254548A Ceased AU2001254548B2 (en) 2000-04-20 2001-04-20 Enhanced stabilised plasmid-lipid particle-mediated transfection using endosomal membrane

Country Status (4)

Country Link
EP (1) EP1355670A2 (xx)
JP (1) JP2004508012A (xx)
AU (2) AU2001254548B2 (xx)
WO (1) WO2001080900A2 (xx)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040048260A1 (en) * 2002-09-10 2004-03-11 Fu-Hsiung Chang Transfection of nucleic acid
CA2540917C (en) * 2003-10-01 2012-03-27 Japan Science And Technology Agency Polyarginine-modified liposome having nuclear entry ability
CN1968714B (zh) 2004-05-05 2013-10-16 赛伦斯治疗公司 脂质、脂质复合物及其应用
EP1830888B1 (en) 2004-12-27 2015-08-05 Silence Therapeutics GmbH Lipid complexes coated with peg and their use
JP2007063194A (ja) * 2005-08-31 2007-03-15 Tokyo Institute Of Technology 物質導入担体、物質導入方法及び物質導入用キット
CA2649630C (en) * 2006-04-20 2016-04-05 Silence Therapeutics Ag Lipoplex formulations for specific delivery to vascular endothelium
AU2013257401B2 (en) * 2006-04-20 2016-03-17 Silence Therapeutics Gmbh Lipoplex formulations for specific delivery to vascular endothelium
EP2546337A1 (en) 2006-07-21 2013-01-16 Silence Therapeutics AG Means for inhibiting the expression of protein kinase 3
US8877206B2 (en) 2007-03-22 2014-11-04 Pds Biotechnology Corporation Stimulation of an immune response by cationic lipids
JP5971945B2 (ja) 2008-04-17 2016-08-17 ピーディーエス バイオテクノロジー コーポレイションＰｄｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐｏｒａｔｉｏｎ カチオン性脂質の鏡像異性体による免疫応答の刺激
US20130022649A1 (en) * 2009-12-01 2013-01-24 Protiva Biotherapeutics, Inc. Snalp formulations containing antioxidants
EP2897639A4 (en) 2012-09-21 2016-05-04 Frank Bedu-Addo IMPROVED VACCINE COMPOSITIONS AND METHODS OF USE
EP3308789A4 (en) * 2015-06-10 2019-04-24 Keio University NUCLEIC ACID-FREEZING CARRIER, NUCLEIC ACID-FREEZING KIT, AND NUCLEIC ACID-FREEZING METHOD
CA3005251A1 (en) 2015-11-13 2017-05-18 Pds Biotechnology Corporation Lipids as synthetic vectors to enhance antigen processing and presentation ex-vivo in dendritic cell therapy
CN111979190A (zh) * 2019-05-23 2020-11-24 南京依莫诺飞生物科技有限公司 一种用于转染t细胞的培养基及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5885613A (en) * 1994-09-30 1999-03-23 The University Of British Columbia Bilayer stabilizing components and their use in forming programmable fusogenic liposomes
CA2222328C (en) * 1995-06-07 2012-01-10 Inex Pharmaceuticals Corporation Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
DE69730218D1 (de) * 1996-04-11 2004-09-16 Univ British Columbia Fusogene liposomen
TW520297B (en) * 1996-10-11 2003-02-11 Sequus Pharm Inc Fusogenic liposome composition and method
WO1998046208A1 (en) * 1997-04-17 1998-10-22 The Regents Of The University Of Michigan Hair follicle dna delivery system
WO1998051285A2 (en) * 1997-05-15 1998-11-19 Genzyme Corporation Cationic amphiphile formulations
CN100567493C (zh) * 1997-06-27 2009-12-09 加利福尼亚大学董事会 具有迟发性种子散布的特性的种子植物
US6395713B1 (en) * 1997-07-23 2002-05-28 Ribozyme Pharmaceuticals, Inc. Compositions for the delivery of negatively charged molecules
WO1999005303A1 (en) * 1997-07-24 1999-02-04 Inex Pharmaceuticals Corporation Preparation of lipid-nucleic acid particles using a solvent extraction and direct hydration method
CA2271582A1 (en) * 1998-05-14 1999-11-14 Sean C. Semple Method for administration of therapeutic agents, including antisense, with repeat dosing
CA2335638A1 (en) * 1998-06-19 1999-12-23 Ronald K. Scheule Cationic amphiphile micellar complexes
JP2002521423A (ja) * 1998-07-31 2002-07-16 コリア インスティチュート オブ サイエンス アンド テクノロージ 遺伝子又は薬物運搬体としての脂質エマルジョン及び固形脂質微粒子
JP2002535290A (ja) * 1999-01-21 2002-10-22 ジョージタウン・ユニバーシティ ターゲッティングした遺伝子移入のためのリガンド−ｐｅｇポストコート安定化したリポプレックスおよびポリプレックス
CA2370690A1 (en) * 1999-04-20 2000-10-26 The University Of British Columbia Cationic peg-lipids and methods of use
CN1228041C (zh) * 1999-07-15 2005-11-23 英耐克斯药品股份有限公司 脂质包埋的治疗剂的制备方法

Also Published As

Publication number Publication date
WO2001080900A2 (en) 2001-11-01
EP1355670A2 (en) 2003-10-29
WO2001080900A3 (en) 2003-04-24
JP2004508012A (ja) 2004-03-18
AU2001254548B2 (en) 2006-09-28

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