AU4049099A

AU4049099A - Novel compounds derived from alpha-d-xylose, preparation method and therapeutic use

Info

Publication number: AU4049099A
Application number: AU40490/99A
Authority: AU
Inventors: Veronique Barberousse; Alan Dunlap Edgar; Christiane Legendre; Soth Samreth
Original assignee: Fournier Industrie et Sante SAS
Current assignee: Fournier Industrie et Sante SAS
Priority date: 1998-06-24
Filing date: 1999-06-11
Publication date: 2000-01-10
Anticipated expiration: 2019-06-11
Also published as: DE69903727D1; EP1090017A1; ATE226956T1; AR019701A1; EE200000752A; HUP0102772A2; BR9911585A; PL345068A1; KR20010053038A; IL140417A0; HK1039128A1; NO20005932L; CA2335799A1; TR200003797T2; AU746680B2; SK20122000A3; ID26806A; WO1999067261A1; CN1305486A; HUP0102772A3

Description

New derivative compounds of a-D-xylose, preparation 5 and use in therapeutics Field of invention The present invention concerns, as new industrial products, the derivatives of a-D xylose defined by formula I below. It also concerns the process for the preparation of these 10 compounds, as well as the therapeutic compositions containing them as active ingredients. Prior art Derivatives of p-D-xylose, in particular derivatives of benzoyl- or a-hydroxy-benzyl phenyl P-D-xylosides, recommended in therapeutics for the treatment of venous thromboses, 15 are already known, for example, according to EP-A-0051023. Derivatives of benzyl-phenyl P-D-xylosides, exhibiting a hypocholesterolemiant and/or hypolipidemiant activity, are also known according to EP-A-0133103. Derivatives of the type p-D-phenylthioxylosides, used for their antithrombotic activity, are also known according to EP-A-0365397, EP-A-0290321, EP-A-0133103 and EP-A 20 0051023. The antithrombotic activity of a certain number of derivatives of P-D-xylose has also been reported and studied in the article of J. Med. Chem, 1993, 36, (no. 7) pages 898-903. Research carried out in the laboratory has shown that these derivatives of P-D-xylose are good substrates of galactosyl transferase 1. For this reason, these compounds, active when 25 taken orally, initiate the synthesis of glycosaminoglycanes (GAGs). After administration of the compounds orally, the circulation rates of GAGs are appreciably increased and approximately 20% of the latter display an activity of the dermatan-sulphate type capable of inhibiting thrombin, via HC II (Heparin Cofactor 11), this initiation of the biosynthesis of GAGs probably being responsible for the antithrombotic activity observed experimentally for the 30 compounds mentioned previously. In correlation with the potential of these compounds to reduce the formation of venous thromboses, only the P configuration derivatives of D-xylose increase the synthesis of GAGs. The other derivatives of the glycopyranoside type have proved to be inactive in this area, both from the biological standpoint on the synthesis of GAGs, as well as from the pharmacological standpoint on the reduction or prevention of 35 venous thromboses.

The activity of certain derivatives of p-D-xylose, in particular estradiol P-D-xyloside, has been studied in the publication Journal of Biological Chemistry, 1991, 266, (No. 11) pages 6674-6677 and the authors establish a relationship between this compound and the biosynthesis of heparane sulphate, as well as an initiator role of P-D-xyloside in the synthesis 5 of chondroitin sulphate. These studies confirm the benefit of derivatives of p-D-xylose for the treatment or the prevention of venous thromboses. Aim of the invention According to the invention, it is proposed to provide a new technical solution making it possible to arrive at new products that are biologically beneficial with respect to arterial 10 atheromatous platelets. Subject-matter of the invention According to the new technical solution of the invention, use is made of products of the type a-D-xylose or a-D-thioxylose. It has in fact been found, in a surprising manner, that derivatives of D-xylose or of 5 15 thio-D-xylose no longer displaying the P configuration, but the a configuration on the anomeric carbon, possess a particularly beneficial activity for the prevention or the regression of arterial atheromatous platelets. The new products according to the invention, which are compounds of a-D-xylose, are characterised in that they are selected from the compounds of the general formula I: 20 X OR OR OR & R wherein X and Y represent, independently of one another, an oxygen atom or a sulphur atom,

R

1 represents a CN, CF 3 or SO 2

CH

3 group, and 25 R represents a hydrogen atom or an aliphatic acyl group containing 2 to 5 carbon atoms. According to another aspect of the invention, a composition is provided that is characterised in that it contains, in association with a physiologically acceptable excipient, a therapeutically effective quantity of at least one compound of the formula 1. It is also recommended to use the compound of the formula I as an 30 antiatheromatous drug.

Detailed description of the invention As presented in formula I above, the compounds according to the invention are derivatives of a-D-xylose or a-D-thioxylose, substituted on the anomeric carbon in the a position by a substituted benzophenone group. 5 The hydroxyl functions of D-xylose or D-thioxylose can be free or substituted by an acyl group containing 2 to 5 carbon atoms, preferably the acetyl group. Aliphatic acyl group containing 2 to 5 carbon atoms is understood here to mean an acyl group with a straight, branched or cyclised chain, such as in particular

CH

3 CO,

CH

3

CH

2 CO, (CH 3

)

2 CHCO, (CH 3

)

3 CCO, or cyclopropyl-carbonyl. 10 The compounds of the formula I can be prepared according to a method known per se by using conventional reactive mechanisms. According to a preferred operating method, a benzophenone of the formula 11 is reacted (according to a coupling reaction): H-Y C

R

1 15 wherein Y is the oxygen atom or the sulphur atom and R 1 represents CN, CF 3 or S02CH 3 , with a compound of D-xylose (or of 5-thio-D-xylose) of the formula IlIl: x OAc O-Z (III) OAc OAc where-,v* represents a bond of indeterminate configuration (a,p or a/ P mixture), X is an 20 oxygen atom or a sulphur atom, Ac represents the acetyl group, Z represents an acetyl group or a trichloromethylimino group [-C(=NH)-CC 3 ], the reaction being conducted in a solvent and in the presence of a Lewis acid, in order to obtain a compound of the formula I after purification: _X OR OR OR wherein X, Y and R 1 retain the same significance as in the starting products and R represents an acetyl group. 5 The compounds of the formula I in which R is a hydrogen atom can be obtained from the preceding compounds, in which R is the acetyl group, by the action of a base such as for example sodium methylate or ammonia which permit the acetyl group to be replaced by a hydrogen atom. The compounds of the formula I in which R is a C2 -C5 acyl group, such as defined 10 above, can be obtained from compounds with formula I in which R is a hydrogen atom, by the action of chloride or acid anhydride corresponding to the desired C 2

-C

5 acyl group, in the presence of an aprotic base such as for example triethylamine or pyridine, and in a solvent such as for example dichloromethane. Briefly, the process of preparation according to the invention comprises (a) the II + 15 Ill coupling reaction and the purification of the compound I (R = Ac) thus obtained, (b) if necessary the hydrolysis (saponification) of said compound of the formula I (R = Ac) so as to obtain the deacetylated compound with formula I (R = H), and (c) if necessary the esterification of said compound of the formula I (R = H) so as to obtain any other esterified compound of the formula I (R = acyl, in particular an acyl group different from Ac). 20 The compounds of the formula I are useful in therapeutics as active principles of drugs intended for the treatment or prevention of atherosclerosis. According to the invention, it is recommended to use a product selected from the group consisiting of the compounds of the formula I above for the preparation of an antiatheromatous drug intended for use in therapeutics with respect to atherosclerosis. 25 The preferred products according to the invention, in view of the beneficial properties with respect to atherosclerosis, are the compounds of the formula I where R 1 is CN, and among the latter the products in which X = Y = S or X = Y = 0. The following non-limiting examples of preparation permit the advantages of the invention to be better understood and appreciated.

Example I

[

4 -(4-cyanobenzoyl)phenyl] 2,3,4-tri-0-acetyl-1, 5 -dithio-a-D-xylopyranoside A suspension of 5 g (15. 10~3 mol) of tetra-O-acetyl-5-thio-D-xylopyranose, 4.3 g (18 S10-3 mol) of 4

-(

4 -mercaptobenzoyl)benzonitrile and 5 g of 4 A molecular sieve is prepared 5 in 100 ml of acetonitrile and 4 ml of boron trifluoride etherate (at 48%, d = 1.13) is added at 0*C while stirring. The reaction mixture is allowed to return to room temperature and stirring is carried on for one hour. The mixture is then filtered, then concentrated under reduced pressure. The residue is dissolved in ethyl acetate and the solution is washed with diluted soda, then with diluted hydrochloric acid and finally with water until neutrality. The organic 10 phase is dried and concentrated under reduced pressure. The residue is purified by chromatography on silica gel, eluting with a toluene/ethyl acetate mixture (6/1; v/v). 7 g of the sought compound is thus obtained in a mixture with the P isomer (the two isomers are in the ratio a/ P = 60/40). This mixture is dissolved in 15 ml of ethyl acetate and 15 ml of ethyl ether is added. The crystals obtained (essentially the P isomer) are eliminated by filtration and the 15 filtrate is concentrated under reduced pressure. 5.4 g of the sought compound is thus obtained containing 20% of the P isomer (yield = 63%). The sought compound is obtained with 98% purity in the a isomer after two recrystallisations in methanol. F = 144-146*C [a]24D = + 3280 (c = 0.42; CH 2 Cl 2 ) 20 Example 2

[

4 -(4-cyanobenzoyl)phenyl] 1, 5 -dithio-a-D-xylopyranoside A solution of 10.27 g (20. 10-3 mol) of the compound obtained according to example 1 is prepared in 100 ml of methanol and 35 ml of tetrahydrofuran. 1.15 ml (4 . 10-3 mol) of a solution of sodium methylate is then added at 10-150C. 25 After 20 min. while stirring at 10-15*C, the reaction mixture is percolated on an IR 120 resin (H*). The solution is decolourised by means of activated carbon, filtered and concentrated under reduced pressure. The residue is crystallised in methanol. After recrystallisation in methanol, 5 g of the sought product is obtained in the form of white crystals (yield = 65%). F = 142*C 30 [a]D 20 = + 5080 (c = 0.42; CH 3 0H) Example 3

[

4 -(4-cyanobenzoyl)phenyl] 2

,

3

,

4 -tri-O-acetyl-a-D-xylopyranoside A mixture of 9 g (28.3. 10- 3 mol) of tetra-O-acetyl-D-xylose and 8 g (35.9. 10-3 mol) of 4

-(

4 -hydroxybenzoyl)benzonitrile is prepared in 90 ml of dichloromethane. 8 ml (68.4 . 10-3 35 mol) of tin tetrachloride is then added drop by drop at room temperature, then the reaction medium is brought to 450C, for 15 hours, while stirring. The mixture is then poured onto ice and diluted hydrochloric acid; extraction is then performed with ethyl acetate in acid medium, then the united organic phases are washed with a diluted solution of hydrochloric acid, then with water, with a diluted soda solution, then with water. The organic phase is dried on 5 magnesium sulphate then concentrated under reduced pressure. The raw product is purified by chromatography on silica gel, eluting with the aid of a toluene/ethyl acetate mixture (5/1 ; v/v). 3.3 g of the sought product is thus obtained in the form of a white powder (yield = 24%). F = 750C 10 [a]D 26 = + 146.3*(c = 0.36; CH 3 0H) Example 4 [4-(4-cyanobenzoyl)phenyl] a-D-xylopyranoside 4.3 g (8.94 . 10-3 mol) of the compound obtained according to example 3 is added to 50 ml of a saturated solution of ammonia in methanol, at 00C, and the mixture is held for 15 3 hours at O'C while stirring, then for 2 hours at room temperature. The reaction medium is then concentrated under reduced pressure and the residue is purified by chromatography on silica gel, eluting with a dichloromethane/methanol mixture (10/1; v/v). The pure fraction obtained is concentrated to eliminate the solvents, then the product is put into suspension in distilled water; the mixture is congealed and freeze-dried. 2.15 g of the sought product is 20 thus obtained in the form of a crushed white powder (yield = 67%). F = 186-187*C [C]2 D = + 1550 (c = 0.40; CH 3 0H) Example 5 [4-(4-cyanobenzoyl)phenyl] 2,3,4-tri-O-acetyl-1 -thio-a-D-xylopyranoside 25 Operating in a similar manner to example 3, starting from 4-(4-mercapto benzoyl)benzonitrile, the sought product is obtained in the form of a white solid (yield = 11%). F = 158-159*C [a]26D = + 1610 (c = 0.38; CH2Cl2) Example 6 30 [4-(4-cyanobenzoyl)phenyl] 1-thio-a-D-xylopyranoside Operating in a similar manner to example 4, starting from the compound obtained according to example 5, the sought product is obtained in the form of a cream powder (yield = 90%). F = 1980C 35 [a]2eD = + 2450 (c = 0.30; CH 3 0H) Example 7 [4-(4-cyanobenzoyl)phenyl] 2,3,4-tri-O-acetyl-5-thio-a-D-xylopyranoside) A suspension of 2 g (9 . 10-3 mol) of 4-(4-hydroxy-benzoyl)benzonitrile is prepared in 40 ml of dichloromethane, 1 g of 4 A molecular sieve is added and the mixture is cooled 5 to -30*C. 1.8 ml (8. 10 3 mol) of triethylsilyl trifluoromethanesulphonate is slowly added while stirring, then a solution of 3.9 g (9 . 10- mol) of 2,3,4-tri-O-acetyl-5-thio-a-D-xylopyranosy trichloroacetimidate in 20 ml of dichloromethane. The reaction mixture is stirred for 4 hours at -30*C then for 16 hours at 00C. After neutralisation with the aid of a collodine solution, the reaction medium is filtered. The filtrate is diluted in 200 ml of ethyl acetate and the organic 10 phase obtained is washed with a solution of diluted soda, then with water, with the aid of a diluted hydrochloric acid solution, then with water and dried on magnesium sulphate. After concentration of the solution under reduced pressure, the residue is purified by chromatography on silica gel, eluting with a methylcyclohexane-ethyl acetate mixture (5/2; v/v). 0.86 g of the sought product is thus obtained in the form of a beige powder 15 (yield = 19%). F = + 850C [a]21 = + 365* ( c = 0.3; CH 2 Cl 2 ) Example 8 [4-(4-cyanobenzoyl)phenyl] 5-thio-a-D-xylopyranoside 20 Operating in a similar manner to example 4, starting from the compound obtained according to example 7, the sought product is obtained in the form of a clear beige powder (yield = 73 %). F = 1800C [a]2D = + 4760 (c = 0.32; CH 3 0H) 25 Example 9 [4-[4-(trifluoromethyl)benzoyl]phenyl] 2,3,4-tri-O-acetyl-c-D-xylopyranoside A solution of 2.5 g (9.4 . 103 mol) of (4-hydroxy-phenyl)[4-(trifluoromethyl)phenyl] methanone and 3.5 g (11 . 103 ) of tetra-O-acetyl-D-xylose is prepared in 30 ml of acetonitrile and I g of 4 A molecular sieve is added. The mixture is cooled to 00C and 7.2 ml (58 . 10 30 mol) of boron trifluoride etherate is added drop by drop while stirring. The reaction medium is then held at room temperature for 15 hours while stirring, then it is filtered. The filtrate is diluted with ethyl acetate and the organic phase is washed with a solution of diluted soda, with water, with a solution of diluted hydrochloric acid and again with water. After drying on magnesium sulphate, the organic phase is concentrated under reduced pressure and the 35 residue is purified by chromatography on silica gel, eluting with the aid of a methylcyclohexane-ethyl acetate mixture (5/2; v/v). 0.46 g of the sought product is thus obtained in the form of an amorphous beige solid (yield = 10%). F = 65'C [a]26D = + 1410 ( c = 0.32; CH 2

CI

2 ) 5 Example 10

[

4

-[

4 -(trifluoromethyl)benzoyl]phenyl] a-D-xylopyranoside 0.370 g (0.7 . 10~3 mol) of the compound obtained according to example 9 is dissolved in 20 ml of methanol and 40 pl of a solution of sodium methylate at 25% in the methanol is added . The solution is held for two hours while stirring, then approx. 0.5 g of 120 10 IR resin is added in acidic form. After filtration, the filtrate is concentrated under reduced pressure. 240 mg of the sought product is thus obtained in the form of a beige solid (yield = 85%). F = 188*C [a] 21 D = + 1650 ( c = 0.27; CH 3 0H) 15 Example 11

[

4

-[

4 -(trifluoromethyl)benzoyl]phenyl] 2,3,4-tri-0-acetyl-5-thio-a-D-xylopyranoside Operating in a similar manner to example 7, starting from (4-hydroxyphenyl)[4 (trifluoromethyl)phenyl]methanone, the sought product is obtained in the form of a beige amorphous solid (yield = 19.5%). 20 F = 65C [aD = + 3200 (c = 0.34; CH 2

CI

2 ) Example 12

[

4

-[

4 -(trifluoromethyl)benzoyl]phenyl] 5-thio-a-D-xylopyranoside Operating in a similar manner to example 10, starting from the compound obtained 25 according to example 11, the sought product is obtained in the form of an amorphous white powder (yield = 80%). F = 820C [a] 22 D = + 378* (c = 0.25; CH 3 0H) PREPARATION I 30 Dimethylcarbamothioic acid, 0-[ 4

-[

4 -(trifluoromethyl)benzoyl]phenyl] ester A solution of 10 g (37.6 .10~3 mol) of ( 4 -hydroxy-phenyl)[4-(trifluoromethyl)phenyl] methanone is prepared in 100 ml of acetone and a solution of 2.94 g (45 . 10-3 mol) of potassium hydroxide (at 80 %) in 80 ml of water is slowly added while stirring and at room temperature. A solution of 5.11 g (41.3 .10-3 mol) of dimethylthiocarbamoyl chloride in 70 ml 35 of acetone is then added. The reaction mixture is held at room temperature for 8 hours while stirring, then concentrated at reduced pressure. The raw product is put into suspension in 60 ml of a 1 mol/ solution of potassium hydroxide and stirred for 20 min. at 10-150C. The solid is filtered, rinsed in suspension with water until a neutral pH , then dried in the drier. 10.3 g of the product sought is thus obtained in the form of a beige powder (yield = 78%). 5 F = 174 0 C PREPARATION II Dimethylcarbamothioic acid, S-[4-[4-(trifluoromethyl)benzoyl]phenyl] ester 10.3 g (29 .10- mol) of the compound obtained according to preparation I is placed in a flask, under an atmosphere of nitrogen, and the product is held at 250-2600C for 1 hour. 10 After cooling, approx. 15 ml of ethyl acetate is added, the mixture is brought to a slight reflux, then left to cool down to 00C. After approx. 10 hours at this temperature, the crystallised solid is filtered and the solid is rinsed with approx. 10 ml of cyclohexane. After drying in the drier, 6.2 g of the product sought is obtained in the form of beige crystals (yield = 60%). F = 140'C 15 PREPARATION III (4-mercaptophenyl)-[4-(trifluoromethyl)phenyl]methanone A suspension of 6.1 g (17.3 .10-3 mol) of the compound obtained according to preparation 11 is prepared in 65 ml of methanol. After having deoxygenated the medium by bubbling-through with nitrogen, 10 ml (i.e. 34 .10-3 mol) of a solution of sodium methylate in 20 methanol is added , then the reaction mixture is raised to 400C while stirring for three hours. Partial concentration is then carried out (approx. 25 ml of methanol is eliminated) under reduced pressure and the solution is poured into a mixture of ice and diluted hydrochloric acid. The sought product precipitates. The solid product is separated by filtration and washed with water until neutral pH. After drying in the drier, 4.8 g of the product sought is obtained 25 in the form of a clear green solid. F = 1500C Example 13 [4-[4-(trifluoromethyl)benzoyl]phenyl] 1-thio-2,3,4-tri-O-acetyl-a-D-xylopyranoside Operating in a similar manner to example 9, starting from the compound obtained 30 according to preparation l1l, the sought product is obtained in the form of a beige solid (yield = 27%). F = 600C [a]2D = + 1450 ( c = 0.25; CH 2 Cl 2

)

Example 14 [4-[4-(trifluoromethyl)benzoyl]phenyl] 1-thio-a-D-xylopyranoside Operating in a similar manner to example 10, starting from the compound obtained according to example 13, the sought product is obtained in the form of a cream amorphous 5 solid (yield = 95%). F = 1720C [a] 24 D = + 2110 (c = 0.35; CH 3 0H) Example 15 [4-[4-(trifluoromethyl)benzoyl]phenyl] 2,3,4-tri-O-acetyl-1,5-dithio-ax-D-xylopyranoside 10 Operating in a similar manner to example 9, starting from 1,2,3,4-tetra-O-acetyl-5 thio-D-xylose and the compound obtained according to preparation Ill, the sought product is obtained in the form of a white powder (yield = 18%). F = 700C a]27D = + 2150 (c = 0.45; CH 2

CI

2 ) 15 Example 16 [4-[4-(trifluoromethyl)benzoyl]phenyl] 1,5-d ith io-c-D-xylopyranoside Operating in a similar manner to example 10, starting from the compound obtained according to example 15, the sought product is obtained in the form of a white powdery solid (yield = 84%). 20 F = 104 0 C [a]2D = + 3990 (c = 0.50; DMSO) PREPARATION IV (4-hydroxyphenyl)[4-(methylsulphinyl)phenyl]methanone A solution of 15 g (61.4 .10-3 mol) of (4-hydroxyphenyl)[4-(methylthio) 25 phenyl]methanone is prepared in 200 ml of methanol. This is cooled down to approx. 50C with the aid of an ice bath and14.12 g (61.4 . 10-3 mol) of 3-chloro-benzenecarboperoxoic acid (mCPBA) is added by fractions titrating 75%. The reaction medium is kept stirring for 15 min. after completion of the addition and hydrolysis is performed on a diluted solution of sodium bicarbonate. Extraction is performed with the aid of ethyl acetate and the phase obtained is 30 washed with water until neutrality, dried and concentrated under reduced pressure. 13.2 g of a white solid composed of the sought product is thus obtained in a mixture with (4-hydroxyphenyl)[4-(methylsulphonyl)phenyl]methanol. PREPARATION V (4-hydroxyphenyl)[4-(methylsulphonyl)phenyl]methanone 35 A suspension of 13.2 g of the compound obtained according to preparation IV is prepared in 150 ml of methanol and 11.7 g (50.8. 10- mol) of mCPBA is added by portions while stirring (titrating 75%). The reaction mixture is kept stirring for 30 minutes, then hydrolysis is performed on a cold solution of sodium bicarbonate. Extraction is performed with the aid of ethyl acetate and the organic phase is washed with water until neutrality, dried then 5 concentrated under reduced pressure. 13.6 g of the product sought is thus obtained in the form of a white solid (yield = 97%). F = 1440C PREPARATION VI Dimethylcarbamothioic acid, 0-[4-[4-(methylsulphonyl)benzoyl]phenyl] ester 10 Operating in a similar manner to preparation I, starting from the compound obtained according to preparation V, the sought product is obtained in the form of a beige solid (yield = 69%). F = 1500C PREPARATION VII 15 Dimethylcarbamothioic acid, S-[4-[4-(methylsulphonyl)benzoyl]phenyl] ester Operating in a similar manner to preparation 11, starting from the compound obtained according to preparation VI, the sought product is obtained in the form of beige crystals (yield = 91%). F = 1720C 20 PREPARATION VIII (4-mercaptophenyl)[4-(methylsulphonyl)phenyl] methanone Operating in a similar manner to preparation 1Il, starting from the compound obtained according to preparation VII (adding dimethyl formamide to solubilise the product), the sought product is obtained in the form of a cream solid (yield = 95%). 25 F = 176*C Example 17 [4-[4-(methylsulphonyl)benzoyl]phenyl] 2,3,4-tri-O-acetyl-1,5-dithio-a-D-xylopyranoside Operating in a similar manner to example 15, starting from the compound obtained according to preparation VIII, the sought product is obtained in the form of a beige solid 30 (yield = 31 %). F = 86*C [a]2o = + 293* (c = 0.44; DMSO) Example 18 [4-[4-(methylsulphonyl)benzoyl]phenyl] 1,5-dithio-a-D-xylopyranoside 35 Operating in a similar manner to example 10, starting from the compound obtained according to example 17, the sought product is obtained in the form of a pale yellow solid (yield = 90.5 %). F = 1000C [Ca] 2 6 D = + 3810 (c = 0.58; DMSO) 5 The antiatheromatous activity of the compounds according to the invention has been demonstrated on female mice, deficient in apolipoprotein E (homozygotes). According to the report on this test, the product to be evaluated is administered in the food (standard diet) for 14 weeks. After this'space of time, the mice undergo euthanasia and samples are taken 10 from the heart and the aortic arch. The damaged areas are evaluated according to the method described in Arteriosclerosis, 1990, 10, p. 316-323. Table I shows the results obtained according to this test carried out with the compound of example 2 at difference doses. The results are expressed in percentage reduction of the area of the atheroma platelets, as compared with a group of untreated 15 control mice. Table 1 dose administered (mg/kg) 10 100 300 reduction (%) - 32 - 51 - 71 20 By way of comparison, the compound [4-(4-cyanobenzoyl)phenyl] 1,5-dithio-p-D xylopyranoside, previously described in EP-A-0290321, was also tested. In the dose of 300 mg/kg, this compound reduces the damaged area by 30%. As shown in table 1, the same biological result is achieved with only 10 mg/kg of the compound according to example 2. This demonstrates the superiority of the compound of the invention in this activity when the 25 anomeric carbon of the xylose is in the a configuration. During the 14 weeks of the test described above, aimed at evaluating the antiatheromatous properties of the compounds, the serum cholesterol rate of the treated mice and the control mice was measured, and the result is expressed in the form area-under curve (AUC) of cholesterol during the whole duration of the test. The results obtained with 30 the compound of example 2 are entered in table II where AUC represents the area-under curve (expressed in g.day.1 1 ) and the variation (in percentage) is evaluated by reference to the control group. Table II dose (mg/kg) 0 10 100 300 (Ex 2) (control) AUC 583 23 585 ±15 507 ±16 505 ±20 variation (%) - 0 - 13 - 13 5 The analysis of the values observed reveals a hypocholesterolemiant effect which only appears over the period of 14 weeks with the 100 mg/kg dose whereas the antiatheromatous effect is significant from the 10 mg/kg dose. However, in view of the probability of a correlation between the antiatheromatous 10 activity observed and a capacity of the compound to lower the serum cholesterol rate, and in order to obtain a screening result as quickly as possible, the compounds according to the invention were evaluated according to their potential for diminishing the cholesterolaemia of mice subjected to a diet rich in fats. The test was performed by administration to female mice of strain C57BL/6J. The report is as follows: on the first day (JO), the mice undergo fasting 15 from the 9.00 to 17.00 hours, a blood sample being taken at 14.00 hours. At 17.00 hours, a determined amount of food (fatty diet containing 1.25% of cholesterol and 0.5% of cholic acid) is distributed. On the second day (J1), at 9.00 hours, the remains of the food are weighed and the mice undergo fasting from 9.00 to 14.00 hours. At 14.00 hours, a blood sample is taken. For the groups of treated mice, the compound is administered by tubing, 20 in suspension in a solution of gummy water, at 3%, on the second day (J1) at 9.00 hours. The control groups only receive the gummy water. The compounds were tested at the dose of 100 mg/kg. The serum total cholesterol is analysed and the results are expressed in percentage inhibition of the increase in cholesterolaemia as compared with the control group. The results obtained are entered in the 25 column "Activity" of table Ill. Moreover, it can be seen that the analysis of the cholesterol content of the various classes of serum lipoproteins reveals a favourable effect of the product on the HDL-cholesterol/total cholesterol ratio.

The products with formula I according to the invention can be administered, preferably orally, in the form of tablets or capsules, each containing 20 to 500 mg of a compound with formula I as the active principle, in association with excipients. The posology will be about 1 to 4 doses per day. These products will be prescribed for the prevention or 5 treatment of atheromatous risk.

Table ll OR Y C/ R OR Ex R1 X Y R Activity (%) 1 CN S S Ac -40 2 CN S S H -38 3 CN 0 0 Ac -46 4 CN 0 0 H -39 5 CN 0 S Ac -41 6 CN 0 S H -38 7 CN S 0 Ac -26 8 CN S 0 H -12 9 CF 3 0 0 Ac 10 CF 3 0 0 H -5 11 CF 3 S 0 Ac -23 12 CF 3 S 0 H -7 13 CF 3 0 S Ac -24 14 CF 3 0 S H -26 15 CF 3 S S Ac -17 16 CF 3 S S H -19 17 S02CH 3 S S Ac -36 18 S02CH 3 S S H 5

Claims

1. An a-D-xylose compound characterised in that it is selected from the group 5 consisting of the compounds of the formula I: X OR OR OR wherein X and Y represent, independently of one another, an oxygen atom or a sulphur atom, 10 R 1 represents a CN, CF 3 or SO 2 CH 3 group, and R represents a hydrogen atom or an aliphatic acyl group containing 2 to 5 carbon atoms.

2. An a-D-xylose compound of the formula I according to claim 1, in which R 1 is CN.

3. An a-D-xylose compound of the formula I according to claim 1, in which X = Y = S 15 and R 1 is CN.

4. An a-D-xylose compound of the formula I according to claim 1, in which X = Y = 0 and R 1 is CN.

5. A method for preparing a compound of the formula I according to claim 1, characterised in that 20 (a) a benzophenone compound of the formula 1l: H-Y / R (1) wherein Y is the oxygen atom or the sulphur atom and R 1 represents CN, CF 3 or SO 2 CH 3 , 25 is reacted with a D-xylose compound of the formula IlIl: x OAc O-Z (III) OAc OAc wherein X is an oxygen atom or a sulphur atom, 5 Ac represents the acetyl group and Z represents an acetyl group or a trichloromethylimino group [-C(=NH)-CCl 3 ], the reaction being conducted in a solvent and in the presence of a Lewis acid, in order to obtain after purification a compound of the formula I: X OR 10 wherein X, Y and R 1 retain the same significance as in the starting products and R represents an acetyl group. (b) if necessary, the compound of the formula 1, thus obtained and in which R is the acetyl 15 group, is reacted with a base in order to replace the acetyl group with a hydrogen atom and to obtain the compound of the formula I in which R is a hydrogen atom, and (c) if necessary, the compound of the formula 1, thus obtained and in which R is a hydrogen atom, is reacted with a C2 -C5 acid chloride or an anhydride of a C2 -C5 aliphatic acid, in the presence of an aprotic base and in a solvent, in order to obtain the compound of the formula 20 1 in which R is an acyl group.

6. A pharmaceutical composition characterised in that it contains, in association with a physiologically acceptable excipient, a therapeutically effective quantity of at least one compound of the formula I according to claim 1.

7. A use of a product selected from the group consisting of the compounds of the 25 formula I according to claim 1, for the preparation of an antiatheromatous drug intended for use in therapeutics vis-a-vis atherosclerosis.