AU3391300A

AU3391300A - Embryonic or stem-like cell lines produced by cross species nuclear transplantation

Info

Publication number: AU3391300A
Application number: AU33913/00A
Authority: AU
Inventors: Jose Cibelli; James Robl; Steven L. Stice
Original assignee: University of Massachusetts UMass
Current assignee: University of Massachusetts UMass
Priority date: 1999-03-02
Filing date: 2000-03-02
Publication date: 2000-09-21
Also published as: WO2000052145A3; IL145151A0; WO2000052145A2; CA2364415A1; EP1159404A2; BR0008714A; JP2002537803A; NZ513888A

Description

WO 00/52145 PCT/USOO/05434 EMBRYONIC OR STEM-LIKE CELL LINES PRODUCED BY CROSS SPECIES NUCLEAR TRANSPLANTATION 5 CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation-in-part of application Serial No. 09/030,945, filed March 2, 1998, which in turn in is a continuation-in-part of application Serial No. 08/699,040, filed August 19, 1996. This application is incorporated by reference in its 10 entirety herein. FIELD OF THE INVENTION The present invention generally relates to the production of embryonic or stem like cells by transplantation of cell nuclei derived from animal or human cells into enucleated animal oocytes of a species different from the donor nuclei. The present 15 invention more specifically relates to the production of primate or human embryonic or stem-like cells by transplantation of the nucleus of a primate or human cell into an enucleated animal oocyte, e.g., a primate or ungulate oocyte and in a preferred embodiment a bovine enucleated oocyte. The present invention further relates to the use of the resultant embryonic or 20 stem-like cells, preferably primate or human embryonic or stem-like cells for therapy, for diagnostic applications, for the production of differentiated cells which may also be used for therapy or diagnosis, and for the production of transgenic embryonic or transgenic differentiated cells, cell lines, tissues and organs. Also, the embryonic or stem-like cells obtained according to the present invention may themselves be used as 2 5 nuclear donors in nuclear transplantation or nuclear transfer methods for the production of chimeras or clons, preferably transgenic cloned or chimeric animals.

WO 00/52145 PCTIUSOO/05434 -2 BACKGROUND OF THE INVENTION Methods for deriving embryonic stem (ES) cell lines in vitro from early preimplantation mouse embryos are well known. (See, e.g., Evans et al., Nature, 29:154-156 (1981); Martin, Proc. Natl. Acad. Sci., USA, 78:7634-7638 (1981)). ES 5 cells can be passaged in an undifferentiated state, provided that a feeder layer of fibroblast cells (Evans et al., Id.) or a differentiation inhibiting source (Smith et al., Dev. Biol., 121:1-9 (1987)) is present. ES cells have been previously reported to possess numerous applications. For example, it has been reported that ES cells can be used as an in vitro model for differen 10 tiation, especially for the study of genes which are involved in the regulation of early development. Mouse ES cells can give rise to germline chimeras when introduced into preimplantation mouse embryos, thus demonstrating their pluripotency (Bradley et al., Nature, 309:255-256 (1984)). In view of their ability to transfer their genome to the next generation, ES cells 15 have potential utility for germline manipulation of livestock animals by using ES cells with or without a desired genetic modification. Moreover, in the case of livestock animals, e.g., ungulates, nuclei from like preimplantation livestock embryos support the development of enucleated oocytes to term (Smith et al., Biol. Reprod., 40:1027-1035 (1989); and Keefer et al., Biol. Reprod., 50:935-939 (1994)). This is in contrast to 20 nuclei from mouse embryos which beyond the eight-cell stage after transfer reportedly do not support the development of enucleated oocytes (Cheong et al, Biol. Reprod., 48:958 (1993)). Therefore, ES cells from livestock animals are highly desirable because they may provide a potential source of totipotent donor nuclei, genetically manipulated or otherwise, for nuclear transfer procedures. 25 Some research groups have reported the isolation of purportedly pluripotent embryonic cell lines. For example, Notarianni et al., J. Reprod. Fert. Supply , 43:255 260 (1991), report the establishment of purportedly stable, pluripotent cell lines from pig and sheep blastocysts which exhibit some morphological and growth characteristics WO 00/52145 PCT/USOO/05434 -3 similar to that of cells in primary cultures of inner cell masses isolated immunosurgically from sheep blastocysts. (Id.) Also, Notarianni et al., J. Reprod. Fert. Supply , 41:51-56 (1990) discloses maintenance and differentiation in culture of putative pluripotential embryonic cell lines from pig blastocysts. Further, Gerfen et al., 5 Anim. Biotech, 6(1):1-14 (1995) disclose the isolation of embryonic cell lines from porcine blastocysts. These cells are stably maintained in mouse embryonic fibroblast feeder layers without the use of conditioned medium. These cells reportedly differ entiate into several different cell types during culture (Gerfen et al., Id.). Further, Saito et al., Roux's Arch. Dev. Biol., 201:134-141 (1992) report bovine 10 embryonic stem cell-like cell lines cultured which survived passages for three, but were lost after the fourth passage. Still further, Handyside et al., Roux's Arch. Dev. Biol., 196:185-190 (1987) disclose culturing of immunosurgically isolated inner cell masses of sheep embryos under conditions which allow for the isolation of mouse ES cell lines derived from mouse ICMs. Handyside et al. (1987) (Id.), report that under such condi 15 tions, the sheep ICMs attach, spread, and develop areas of both ES cell-like and endoderm-like cells, but that after prolonged culture only endoderm-like cells are evident. (Id.) Recently, Cherny et al., Theriogenology, 41:175 (1994) reported purportedly pluripotent bovine primordial germ cell-derived cell lines maintained in long-term 20 culture. These cells, after approximately seven days in culture, produced ES-like colonies which stain positive for alkaline phosphatase (AP), exhibited the ability to form embryoid bodies, and spontaneously differentiated into at least two different cell types. These cells also reportedly expressed mRNA for the transcription factors OCT4, OCT6 and HES 1, a pattern of homeobox genes which is believed to be expressed by 25 ES cells exclusively. Also recently, Campbell et al., Nature, 380:64-68 (1996) reported the production of live lambs following nuclear transfer of cultured embryonic disc (ED) cells from day nine ovine embryos cultured under conditions which promote the WO 00/52145 PCTUSOO/05434 -4 isolation of ES cell lines in the mouse. The authors concluded based on their results that ED cells from day nine ovine embryos are totipotent by nuclear transfer and that totipotency is maintained in culture. Van Stekelenburg-Hamers et al., Mol. Reprod. Dev., 40:444-454 (1995), 5 reported the isolation and characterization of purportedly permanent cell lines from inner cell mass cells of bovine blastocysts. The authors isolated and cultured ICMs from 8 or 9 day bovine blastocysts under different conditions to determine which feeder cells and culture media are most efficient in supporting the attachment and outgrowth of bovine ICM cells. They concluded based on their results that the attachment and 10 outgrowth of cultured ICM cells is enhanced by the use of STO (mouse fibroblast) feeder cells (instead of bovine uterus epithelial cells) and by the use of charcoal stripped serum (rather than normal serum) to supplement the culture medium. Van Stekelenburg et al reported, however, that their cell lines resembled epithelial cells more than pluripotent ICM cells. (Id.) 15 Still further, Smith et al., WO 94/24274, published October 27, 1994, Evans et al, WO 90/03432, published April 5, 1990, and Wheeler et al, WO 94/26889, published November 24, 1994, report the isolation, selection and propagation of animal stem cells which purportedly may be used to obtain transgenic animals. Also, Evans et al., WO 90/03432, published on April 5, 1990, reported the derivation of purportedly 20 pluripotent embryonic stem cells derived from porcine and bovine species which assertedly are useful for the production of transgenic animals. Further, Wheeler et al, WO 94/26884, published November 24, 1994, disclosed embryonic stem cells which are assertedly useful for the manufacture of chimeric and transgenic ungulates. Thus, based on the foregoing, it is evident that many groups have attempted to produce ES 25 cell lines, e.g., because of their potential application in the production of cloned or transgenic embryos and in nuclear transplantation. The use of ungulate ICM cells for nuclear transplantation has also been reported. For example, Collas et al., Mol. Reprod. Dev., 38:264-267 (1994) disclose nuclear WO 00/52145 PCT/USOO/05434 -5 transplantation of bovine ICMs by microinjection of the lysed donor cells into enucleated mature oocytes. The reference disclosed culturing of embryos in vitro for seven days to produce fifteen blastocysts which, upon transferral into bovine recipients, resulted in four pregnancies and two births. Also, Keefer et al., Biol. Reprod., 50:935 5 939 (1994), disclose the use of bovine ICM cells as donor nuclei in nuclear transfer procedures, to produce blastocysts which, upon transplantation into bovine recipients, resulted in several live offspring. Further, Sims et al., Proc. NatL. Acad. Sci., USA, 90:6143-6147 (1993), disclosed the production of calves by transfer of nuclei from short-term in vitro cultured bovine ICM cells into enucleated mature oocytes. 10 Also, the production of live lambs following nuclear transfer of cultured embryonic disc cells has been reported (Campbell et al., Nature, 380:64-68 (1996)). Still further, the use of bovine pluripotent embryonic cells in nuclear transfer and the production of chimeric fetuses has also been reported (Stice et al., Biol. Reprod., 54:100-110 (1996)); Collas et al, Mol. Reprod. Dev., 38:264-267 (1994). 15 Also, there have been previous attempts to produce cross species NT units (Wolfe et al., Theriogenology, 33:350 (1990). Specifically, bovine embryonic cells were fused with bison oocytes to produce some cross species NT units possibly having an inner cell mass. However, embryonic cells, not adult cells were used, as donor nuclei in the nuclear transfer procedure. The dogma has been that embryonic cells are 20 more easily reprogrammed than adult cells. This dates back to earlier NT studies in the frog (review by DiBerardino, Differentiation, 17:17-30 (1980)). Also, this study involved very phylogenetically similar animals (cattle nuclei and bison oocytes). By contrast, previously when more diverse species were fused during NT (cattle nuclei into hamster oocytes), no inner cell mass structures were obtained. Further, it has never 25 been previously reported that the inner cell mass cells from NT units could be used to form an ES cell-like colony that could be propagated. Also, Collas et al (Id.), taught the use of granulosa cells (adult somatic cells) to produce bovine nuclear transfer embryos. However, unlike the present invention, these WO 00/52145 PCT/USOO/05434 -6 experiments did not involve cross-species nuclear transfer. Also, unlike the present invention ES-like cell colonies were not obtained. Very recently, U.S. Patent No. 5,843,780, issued to James A. Thomson on December 1, 1998, assigned to the Wisconsin Alumni Research Foundation, that 5 purports to disclose a purified preparation of primate embryonic stem cells that are (i) capable of proliferation in an in vitro culture for over one year; (ii) maintain a karyotype in which all chromosomes characteristic of the primate species are present and not noticeably altered through prolonged culture; (iii) maintains the potential to differentiate into derivatives of endoderm, mesoderm and ectoderm tissues throughout 10 culture; and (iv) will not differentiate when cultured on a fibroblast feeder layer. These cells were reportedly negative for the SSEA-1 marker, positive for the SEA-3 marker, positive for the SSEA-4 marker, express alkaline phosphatase activity, are pluripotent, and have karyotypes which include the presence of all the chromosomes characteristic of the primate species and in which none of the chromosomes are altered. Further, 15 these cells are respectfully positive for the TRA-1-60, and TRA-1-81 markers. The cells purportedly differentiate into endoderm, mesoderm and ectoderm cells when injected into a SCID mouse. Also, purported embryonic stem cell lines derived from human or primate blastocytes are discussed in Thomson et al., Science 282:1145-1147 and Proc. Natl. Acad. Sci., USA 92:7844-7848 (1995). 20 Thus, Thomson disclose what purportedly are non-human primate and human embryonic or stem-like cells and methods for their production. However, there still exists a significant need for methods for producing human embryonic or stem-like cells that are autologous to an intended transplant recipient given their significant therapeutic and diagnostic potential. 25 In this regard, numerous human diseases have been identified which may be treated by cell transplantation. For example, Parkinson's disease is caused by degenera tion of dopaminergic neurons in the substantia nigra. Standard treatment for Parkinson's involves administration of L-DOPA, which temporarily ameliorates the loss WO 00/52145 PCT/USOO/05434 -7 of dopamine, but causes severe side effects and ultimately does not reverse the progress of the disease. A different approach to treating Parkinson's, which promises to have broad applicability to treatment of many brain diseases and central nervous system injury, involves transplantation of cells or tissues from fetal or neonatal animals into the 5 adult brain. Fetal neurons from a variety of brain regions can be incorporated into the adult brain. Such grafts have been shown to alleviate experimentally induced behavioral deficits, including complex cognitive functions, in laboratory animals. Initial test results from human clinical trials have also been promising. However, supplies of human fetal cells or tissue obtained from miscarriages is very limited. 10 Moreover, obtaining cells or tissues from aborted fetuses is highly controversial. There is currently no available procedure for producing "fetal-like" cells from the patient. Further, allograft tissue is not readily available and both allograft and xenograft tissue are subject to graft rejection. Moreover, in some cases, it would be beneficial to make genetic modifications in cells or tissues before transplantation. 15 However, many cells or tissues wherein such modification would be desirable do not divide well in culture and most types of genetic transformation require rapidly dividing cells. There is therefore a clear need in the art for a supply of human embryonic or stem-like undifferentiated cells for use in transplants and cell and gene therapies. 20 OBJECTS OF THE INVENTION It is an object of the invention to provide novel and improved methods for producing embryonic or stem-like cells. It is a more specific object of the invention to provide a novel method for producing embryonic or stem-like cells which involves transplantation of the nucleus 25 of a mammalian or human cell into an enucleated oocyte of a different species. It is another specific object of the invention to provide a novel method for producing non-human primate or human embryonic or stem-like cells which involves WO 00/52145 PCT/USOO/05434 transplantation of the nucleus of a non-human primate or human cell into an enucleated animal or human oocyte, e.g., an ungulate, human or primate enucleated oocyte. It is another object of the invention to provide a novel method for producing lineage-defective non-human primate or human embryonic or stem-like cells which 5 involves transplantation of the nucleus of a non-human primate or human cell, e.g., a human adult cell into an enucleated non-human primate or human oocyte, wherein such cell has been genetically engineered to be incapable of differentiation into a specific cell lineage or has been modified such that the cells are "mortal", and thereby do not give rise to a viable offspring, e.g., by engineering expression of anti-sense or ribozyme 10 telomerase gene. It is still another object of the invention to enhance efficiency of nuclear transfer and specifically to enhance the development of preimplantation embryos produced by nuclear transfer by genetically engineering donor somatic cells used for nuclear transfer to provide for the expression of genes that enhance embryonic development, e.g., genes 15 of the MHC I family, and in particular Ped genes such as Q7 and/or Q9. It is yet another object of the invention to enhance the production of nuclear transfer embryos by IVP and more specifically nuclear transfer embryos by genetically altering the donor cell used for nuclear transfer such that it is resistant to apoptosis, e.g., by introduction of a DNA construct that provides for the expression of genes that inhibit 20 apoptosis, e.g., Bcl-2 or Bcl-2 family members and/or by the expression of antisense ribozymes specific to genes that induce apoptosis during early embryonic development. It is still another object of the invention to improve the efficacy of nuclear transfer by improved selection of donor cells of a specific cell cycle stage, e.g., GI phase, by genetically engineering donor cells such that they express a DNA construct 25 encoding a particular cyclin linked to a detectable marker, e.g., one that encodes a visualizable (e.g., fluorescent tag) marker protein.

WO 00/52145 PCT/USOO/05434 -9 It is also an object of the invention to enhance the development of in vitro produced embryos, by culturing such embryos in the presence of one or more protease inhibitors, preferably one or more capsase inhibitors, thereby inhibiting apoptosis. It is another object of the invention to provide embryonic or stem-like cells 5 produced by transplantation of nucleus of an animal or human cell into an enucleated oocyte of a different species. It is a more specific object of the invention to provide primate or human embryonic or stem-like cells produced by transplantation of the nucleus of a primate or human cell into an enucleated animal oocyte, e.g., a human, primate or ungulate enucle 10 ated oocyte. It is another object of the invention to use such embryonic or stem-like cells for therapy or diagnosis. It is a specific object of the invention to use such primate or human embryonic or stem-like cells for treatment or diagnosis of any disease wherein cell, tissue or organ 15 transplantation is therapeutically or diagnostically beneficial. It is another specific object of the invention to use the embryonic or stem-like cells produced according to the invention for the production of differentiated cells, tissues or organs. It is a more specific object of the invention to use the primate or human 20 embryonic or stem-like cells produced according to the invention for the production of differentiated human cells, tissues or organs. It is another specific object of the invention to use the embryonic or stem-like cells produced according to the invention for the production of genetically engineered embryonic or stem-like cells, which cells may be used to produce genetically 25 engineered or transgenic differentiated human cells, tissues or organs, e.g., having use in gene therapies.

WO 00/52145 PCTIUSOO/05434 - 10 It is another specific object of the invention to use the embryonic or stem-like cells produced according to the invention in vitro, e.g. for study of cell differentiation and for assay purposes, e.g. for drug studies. It is another object of the invention to provide improved methods of 5 transplantation therapy, comprising the usage of isogenic or synegenic cells, tissues or organs produced from the embryonic or stem-like cells produced according to the invention. Such therapies include by way of example treatment of diseases and injuries including Parkinson's, Huntington's, Alzheimer's, ALS, spinal cord injuries, multiple sclerosis, muscular dystrophy, diabetes, liver diseases, heart disease, cartilage replace 10 ment, bums, vascular diseases, urinary tract diseases, as well as for the treatment of immune defects, bone marrow transplantation, cancer, among other diseases. It is another object of the invention to use the transgenic or genetically engineered embryonic or stem-like cells produced according to the invention for gene therapy, in particular for the treatment and/or prevention of the diseases and injuries 15 identified, supra. It is another object of the invention to use the embryonic or stem-like cells produced according to the invention or transgenic or genetically engineered embryonic or stem-like cells produced according to the invention as nuclear donors for nuclear transplantation. 20 It is still another object of the invention to use genetically engineered ES cells produced according to the invention for the production of transgenic animals, e.g., non human primates, rodents, ungulates, etc. Such transgenic animals can be used to produce, e.g., animal models for study of human diseases, or for the production of desired polypeptides, e.g., therapeutics or nutripharmaceuticals. 25 With the foregoing and other objects, advantages and features of the invention that will become hereinafter apparent, the nature of the invention may be more clearly understood by reference to the following detailed description of the preferred embodiments of the invention and to the appended claims.

WO 00/52145 PCT/USOO/05434 - 11 BRIEFS DESCRIPTION OF THE FIGURES Figure 1 is a photograph of a nuclear transfer (NT) unit produced by transfer of an adult human cell into an enucleated bovine oocyte. Figures 2 to 5 are photographs of embryonic stem-like cells derived from a NT 5 unit such as is depicted in Figure 1. DETAILED DESCRIPTION OF THE INVENTION The present invention provides a novel method for producing embryonic or stem-like cells, and more specifically non-human primate or human embryonic or stem like cells by nuclear transfer or nuclear transplantation. In the subject application, 10 nuclear transfer or nuclear transplantation or NT are used interchangeably. As discussed supra, the isolation of actual embryonic or stem-like cells by nuclear transfer or nuclear transplantation has never been reported. Rather, previous reported isolation of ES-like cells has been from fertilized embryos. Also, successful nuclear transfer involving cells or DNA of genetically dissimilar species, or more 15 specifically adult cells or DNA of one species (e.g., human) and oocytes of another non related species has never been reported. Rather, while embryos produced by fusion of cells of closely related species, has been reported, e.g., bovine-goat and bovine-bison, they did not produce ES cells. (Wolfe et al, Theriogenology, 33(1):350 (1990).) Also, there has never been reported a method for producing primate or human ES cells 20 derived from a non-fetal tissue source. Rather, the limited human fetal cells and tissues which are currently available must be obtained or derived from spontaneous abortion tissues and from aborted fetuses. Also, prior to the present invention, no one obtained embryonic or stem-like cells by cross-species nuclear transplantation. 25 Quite unexpectedly, the present inventors discovered that human embryonic or stem-like cells and cell colonies may be obtained by transplantation of the nucleus of a human cell, e.g., an adult differentiated human cell, into an enucleated animal oocyte, which is used to produce nuclear transfer (NT) units, the cells of which upon culturing WO 00/52145 PCT/USOO/05434 - 12 give rise to human embryonic or stem-like cells and cell colonies. This result is highly surprising because it is the first demonstration of effective cross-species nuclear transplantation involving the introduction of a differentiated donor cell or nucleus into an enucleated oocyte of a genetically dissimilar species, e.g., the transplantation of cell 5 nuclei from a differentiated animal or human cell, e.g., adult cell, into the enucleated egg of a different animal species, to produce nuclear transfer units containing cells which when cultured under appropriate conditions give rise to embryonic or stem-like cells and cell colonies. Preferably, the NT units used to produce ES-like cells will be cultured to a size 10 of at least 2 to 400 cells, preferably 4 to 128 cells, and most preferably to a size of at least about 50 cells. In the present invention, embryonic or stem-like cells refer to cells produced according to the present invention. The present application refers to such cells as stem like cells rather than stem cells because of the manner in which they are typically 15 produced, i.e., by cross-species nuclear transfer. While these cells are expected to possess similar differentiation capacity as normal stem cells they may possess some insignificant differences because of the manner they are produced. For example, these stem-like cells may possess the mitochondria of the oocytes used for nuclear transfer, and thus not behave identically to conventional embryonic stem cells. 20 The present discovery was made based on the observation that nuclear transplantation of the nucleus of an adult human cell, specifically a human epithelial cell obtained from the oral cavity of a human donor, when transferred into an enucleated bovine oocyte, resulted in the formation of nuclear transfer units, the cells of which upon culturing gave rise to human stem-like or embryonic cells and human 25 embryonic or stem-like cell colonies. This result has recently been reproduced by transplantation of keratinocytes from an adult human into an enucleated bovine oocyte with the successful production of a blastocyst and ES cell line. Based thereon, it is hypothesized by the present inventors that bovine oocytes and human oocytes, and WO 00/52145 PCT/USOO/05434 - 13 likely mammals in general must undergo maturation processes during embryonic development which are sufficiently similar or conserved so as to permit the bovine oocyte to function as an effective substitute or surrogate for a human oocyte. Apparently, oocytes in general comprise factors, likely proteinaceous or nucleic acid 5 in nature, that induce embryonic development under appropriate conditions, and these functions that are the same or very similar in different species. These factors may comprise material RNAs and/or telomerase. Based on the fact that human cell nuclei can be effectively transplanted into bovine oocytes, it is reasonable to expect that human cells may be transplanted into 10 oocytes of other non-related species, e.g., other ungulates as well as other animals. In particular, other ungulate oocytes should be suitable, e.g. pigs, sheep, horses, goats, etc. Also, oocytes from other sources should be suitable, e.g. oocytes derived from other primates, amphibians, rodents, rabbits, guinea pigs, etc. Further, using similar methods, it should be possible to transfer human cells or cell nuclei into human oocytes and use 15 the resultant blastocytes to produce human ES cells. Therefore, in its broadest embodiment, the present invention involves the transplantation of an animal or human cell nucleus or animal or human cell into the enucleated oocyte of an animal species different from the donor nuclei, by injection or fusion, to produce an NT unit containing cells which may be used to obtain embryonic 20 or stem-like cells and/or cell cultures. For example, the invention may involve the transplantation of an ungulate cell nucleus or ungulate cell into an enucleated oocyte of another species, e.g., another ungulate or non-ungulate, by injection or fusion, which cells and/or nuclei are combined to produce NT units and which are cultured under conditions suitable to obtain multicellular NT units, preferably comprising at least 25 about 2 to 400 cells, more preferably 4 to 128 cells, and most preferably at least about 50 cells. The cells of such NT units may be used to produce embryonic or stem-like cells or cell colonies upon culturing.

WO 00/52145 PCT/USOO/05434 -14 However, the preferred embodiment of the invention comprises the production of non-human primate or human embryonic or stem-like cells by transplantation of the nucleus of a donor human cell or a human cell into an enucleated human, primate, or non-primate animal oocyte, e.g., an ungulate oocyte, and in a preferred embodiment 5 a bovine enucleated oocyte. In general, the embryonic or stem-like cells will be produced by a nuclear transfer process comprising the following steps: (i) obtaining desired human or animal cells to be used as a source of donor nuclei (which may be genetically altered); 10 (ii) obtaining oocytes from a suitable source, e.g. a mammal and most preferably a primate or an ungulate source, e.g. bovine, (iii) enucleating said oocytes; (iv) transferring the human or animal cell or nucleus into the enucleated oocyte of an animal species different than the donor cell or nuclei, e.g., by fusion or injection; 15 (v) culturing the resultant NT product or NT unit to produce multiple cell structures; and (vi) culturing cells obtained from said embryos to obtain embryonic or stem-like cells and stem-like cell colonies. Nuclear transfer techniques or nuclear transplantation techniques are known in 20 the literature and are described in many of the references cited in the Background of the Invention. See, in particular, Campbell et al, Theriogenology, 43:181 (1995); Collas et al, Mol. Report Dev., 38:264-267 (1994); Keefer et al, Bio. Reprod., 50:935-939 (1994); Sims et al, Proc. Natl. Acad. Sci., USA, 90:6143-6147 (1993); WO 94/26884; WO 94/24274, and WO 90/03432, which are incorporated by reference in their entirety 25 herein. Also, U.S. Patent Nos. 4,944,384 and 5,057,420 describe procedures for bovine nuclear transplantation. See, also Cibelli et al, Science, Vol. 280:1256-1258 (1998). Human or animal cells, preferably mammalian cells, may be obtained and cultured by well known methods. Human and animal cells useful in the present WO 00/52145 PCT/USOO/05434 - 15 invention include, by way of example, epithelial, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, lymphocytes (B and T lymphocytes), other immune cells, erythrocytes, macrophages, melanocytes, monocytes, mononuclear cells, fibroblasts, cardiac muscle cells, and other muscle cells, 5 etc. Moreover, the human cells used for nuclear transfer may be obtained from different organs, e.g., skin, lung, pancreas, liver, stomach, intestine, heart, reproductive organs, bladder, kidney, urethra and other urinary organs, etc. These are just examples of suitable donor cells. Suitable donor cells, i.e., cells useful in the subject invention, may be obtained from any cell or organ of the body. This includes all somatic or germ cells. 10 Preferably, the donor cells or nucleus would comprise actively dividing, i.e., non quiescent, cells as this has been reported to enhance cloning efficacy. Also preferably, such donor cells will be in the G1 cell cycle. The resultant blastocytes may be used to obtain embryonic stem cell lines according to the culturing methods reported by Thomson et al., Science 282:1145-1147 15 (1998) and Thomson et al., Proc. Natl. Acad. Sci., USA 92:7544-7848 (1995), incorporated by reference in their entirety herein. In the example which follows the cells used as donors for nuclear transfer were epithelial cells derived from the oral cavity of a human donor and adult human keratinocytes. However, as discussed, the disclosed method is applicable to other 20 human cells or nuclei. Moreover, the cell nuclei may be obtained from both human somatic and germ cells. It is also possible to arrest donor cells at mitosis before nuclear transfer, using a suitable technique known in the art. Methods for stopping the cell cycle at various stages have been thoroughly reviewed in U.S. Patent 5,262,409, which is herein 25 incorporated by reference. In particular, while cycloheximide has been reported to have an inhibitory effect on mitosis (Bowen and Wilson (1955) J. Heredity 45: 3-9), it may also be employed for improved activation of mature bovine follicular oocytes when WO 00/52145 PCTUSOO/05434 - 16 combined with electric pulse treatment (Yang et al. (1992) Biol. Reprod. 42 (Suppl. 1): 117). Zygote gene activation is associated with hyperacetylation of Histone H4. Trichostatin-A has been shown to inhibit histone deacetylase in a reversible manner 5 (Adenot et al. Differential H4 acetylation of paternal and maternal chromatin precedes DNA replication and differential transcriptional activity in pronuclei of 1-cell mouse embryos. Development (Nov. 1997) 124(22): 4615-4625; Yoshida et al. Trichostatin A and trapoxin: novel chemical probes for the role of histone acetylation in chromatin structure and function. Bioessays (May, 1995) 17(5): 423-430), as have other 10 compounds. For instance, butyrate is also believed to cause hyper-acetylations of histones by inhibiting histone deacetylase. Generally, butyrate appears to modify gene expression and in almost all cases its addition to cells in culture appears to arrest cell growth. Use of butyrate in this regard is described in U.S. Patent No. 5,681,718, which 15 is herein incorporated by reference. Thus, donor cells may be exposed to Trichostatin A or another appropriate deacetylase inhibitor prior to fusion, or such a compound may be added to the culture media prior to genome activation. Additionally, demethylation of DNA is thought to be a requirement for proper access of transcription factors to DNA regulatory sequences. Global demethylation of 20 DNA from the eight-cell stage to the blastocyst stage in preimplantation embryos has previously been described (Stein et al., Mol. Reprod. & Dev. 47(4): 421-429). Also, Jaenisch et al. (1997) have reported that 5-azacytidine can be used to reduce the level of DNA methylation in cells, potentially leading to increased access of transcription factors to DNA regulatory sequences. Accordingly, donor cells may be exposed to 5 25 azacytidine (5-Aza) previous to fusion, or 5-Aza may be added to the culture medium from the 8 cell stage to blastocyst. Alternatively, other known methods for effecting DNA demethylation may be used.

WO 00/52145 PCTIUSOO/05434 - 17 The oocytes used for nuclear transfer may be obtained from animals including mammals and amphibians. Suitable mammalian sources for oocytes include sheep, bovines, ovines, pigs, horses, rabbits, goats, guinea pigs, mice, hamsters, rats, primates, humans, etc. In the preferred embodiments, the oocytes will be obtained from primates 5 or ungulates, e.g., a bovine. Methods for isolation of oocytes are well known in the art. Essentially, this will comprise isolating oocytes from the ovaries or reproductive tract of a mammal or amphibian, e.g., a bovine. A readily available source of bovine oocytes is slaughterhouse materials. 10 For the successful use of techniques such as genetic engineering, nuclear transfer and cloning, oocytes must generally be matured in vitro before these cells may be used as recipient cells for nuclear transfer, and before they can be fertilized by the sperm cell to develop into an embryo. This process generally requires collecting immature (prophase I) oocytes from animal ovaries, e.g., bovine ovaries obtained at a 15 slaughterhouse and maturing the oocytes in a maturation medium prior to fertilization or enucleation until the oocyte attains the metaphase II stage, which in the case of bovine oocytes generally occurs about 18-24 hours post-aspiration. For purposes of the present invention, this period of time is known as the "maturation period." As used herein for calculation of time periods, "aspiration" refers to aspiration of the immature 20 oocyte from ovarian follicles. Additionally, metaphase II stage oocytes, which have been matured in vivo have been successfully used in nuclear transfer techniques. Essentially, mature metaphase II oocytes are collected surgically from either non-superovulated or superovulated cows or heifers 35 to 48 hours past the onset of estrus or past the injection of human chori 25 onic gonadotropin (hCG) or similar hormone. The stage of maturation of the oocyte at enucleation and nuclear transfer has been reported to be significant to the success of NT methods. (See e.g., Prather et al., Differentiation, 48, 1-8, 1991). In general, previous successful mammalian embryo WO 00/52145 PCTIUSOO/05434 - 18 cloning practices use the metaphase II stage oocyte as the recipient oocyte because at this stage it is believed that the oocyte can be or is sufficiently "activated" to treat the introduced nucleus as it does a fertilizing sperm. In domestic animals, and especially cattle, the oocyte activation period generally ranges from about 16-52 hours, preferably 5 about 28-42 hours post-aspiration. For example, immature oocytes may be washed in HEPES buffered hamster embryo culture medium (HECM) as described in Seshagine et al., Bio. Reprod., 40, 544-606, 1989, and then placed into drops of maturation medium consisting of 50 microliters of tissue culture medium (TCM) 199 containing 10% fetal calf serum which 10 contains appropriate gonadotropins such as luteinizing hormone (LH) and follicle stimulating hormone (FSH), and estradiol under a layer of lightweight paraffin or sili con at 39'C. After a fixed time maturation period, which typically will range from about 10 to 40 hours, and preferably about 16-18 hours, the oocytes will be enucleated. Prior to 15 enucleation the oocytes will preferably be removed and placed in HECM containing 1 milligram per milliliter of hyaluronidase prior to removal of cumulus cells. This may be effected by repeated pipetting through very fine bore pipettes or by vortexing briefly. The stripped oocytes are then screened for polar bodies, and the selected metaphase II oocytes, as determined by the presence of polar bodies, are then used for nuclear 20 transfer. Enucleation follows. Enucleation may be effected by known methods, such as described in U.S. Patent No. 4,994,384 which is incorporated by reference herein. For example, metaphase II oocytes are either placed in HECM, optionally containing 7.5 micrograms per milliliter cytochalasin B, for immediate enucleation, or may be placed in a suitable 25 medium, for example CRlaa, plus 10% estrus cow serum, and then enucleated later, preferably not more than 24 hours later, and more preferably 16-18 hours later. Enucleation may be accomplished microsurgically using a micropipette to remove the polar body and the adjacent cytoplasm. The oocytes may then be screened WO 00/52145 PCT/USOO/05434 - 19 to identify those of which have been successfully enucleated. This screening may be effected by staining the oocytes with 1 microgram per milliliter 33342 Hoechst dye in HECM, and then viewing the oocytes under ultraviolet irradiation for less than 10 seconds. The oocytes that have been successfully enucleated can then be placed in a 5 suitable culture medium. In the present invention, the recipient oocytes will preferably be enucleated at a time ranging from about 10 hours to about 40 hours after the initiation of in vitro maturation, more preferably from about 16 hours to about 24 hours after initiation of in vitro maturation, and most preferably about 16-18 hours after initiation of in vitro 10 maturation. A single animal or human cell or nucleus derived therefrom which is typically heterologous to the enucleated oocyte will then be transferred into the perivitelline space of the enucleated oocyte used to produce the NT unit. The animal or human cell or nucleus and the enucleated oocyte will be used to produce NT units according to 15 methods known in the art. For example, the cells may be fused by electrofusion. Electrofusion is accomplished by providing a pulse of electricity that is sufficient to cause a transient break down of the plasma membrane. This breakdown of the plasma membrane is very short because the membrane reforms rapidly. Essentially, if two adjacent membranes are induced to break down, upon reformation the lipid bilayers 20 intermingle and small channels will open between the two cells. Due to the ther modynamic instability of such a small opening, it enlarges until the two cells become one. Reference is made to U.S. Patent 4,997,384 by Prather et al., (incorporated by reference in its entirety herein) for a further discussion of this process. A variety of electrofusion media can be used including e.g., sucrose, mannitol, sorbitol and phos 25 phate buffered solution. Fusion can also be accomplished using Sendai virus as a fuso genic agent (Graham, Wister Inot. Symp. Monogr., 9, 19, 1969). Also, in some cases (e.g. with small donor nuclei) it may be preferable to inject the nucleus directly into the oocyte rather than using electroporation fusion. Such WO 00/52145 PCT/US00/05434 - 20 techniques are disclosed in Collas and Barnes, Mol. Reprod. Dev., 38:264-267 (1994), and incorporated by reference in its entirety herein. Preferably, the human or animal cell and oocyte are electrofused in a 500 pLm chamber by application of an electrical pulse of 90-120V for about 15 gsec, about 24 5 hours after initiation of oocyte maturation. After fusion, the resultant fused NT units are then placed in a suitable medium until activation, e.g., one identified infra. Typically activation will be effected shortly thereafter, typically less than 24 hours later, and preferably about 4-9 hours later. The NT unit may be activated by known methods. Such methods include, e.g., 10 culturing the NT unit at sub-physiological temperature, in essence by applying a cold, or actually cool temperature shock to the NT unit. This may be most conveniently done by culturing the NT unit at room temperature, which is cold relative to the physiological temperature conditions to which embryos are normally exposed. Alternatively, activation may be achieved by application of known activation 15 agents. For example, penetration of oocytes by sperm during fertilization has been shown to activate prefusion oocytes to yield greater numbers of viable pregnancies and multiple genetically identical calves after nuclear transfer. Also, treatments such as electrical and chemical shock or cycloheximide treatment may also be used to activate NT embryos after fusion. Suitable oocyte activation methods are the subject of U.S. 20 Patent No. 5,496,720, to Susko-Parrish et al., which is herein incorporated by reference. For example, oocyte activation may be effected by simultaneously or sequentially: (i) increasing levels of divalent cations in the oocyte, and (ii) reducing phosphorylation of cellular proteins in the oocyte. 25 This will generally be effected by introducing divalent cations into the oocyte cytoplasm, e.g., magnesium, strontium, barium or calcium, e.g., in the form of an iono phore. Other methods of increasing divalent cation levels include the use of electric shock, treatment with ethanol and treatment with caged chelators.

WO 00/52145 PCT/USOO/05434 -21 Phosphorylation may be reduced by known methods, e.g., by the addition of kinase inhibitors, e.g., serine-threonine kinase inhibitors, such as 6-dimethylamino purine, staurosporine, 2-aminopurine, and sphingosine. Alternatively, phosphorylation of cellular proteins may be inhibited by 5 introduction of a phosphatase into the oocyte, e.g., phosphatase 2A and phosphatase 2B. Activated NT units may be cultured in a suitable in vitro culture medium until the generation of embryonic or stem-like cells and cell colonies. Culture media suitable for culturing and maturation of embryos are well known in the art. Examples of known media, which may be used for bovine embryo culture and maintenance, include Ham's 10 F- 10 + 10% fetal calf serum (FCS), Tissue Culture Medium- 199 (TCM- 199) + 10% fetal calf serum, Tyrodes-Albumin-Lactate-Pyruvate (TALP), Dulbecco's Phosphate Buffered Saline (PBS), Eagle's and Whitten's media. One of the most common media used for the collection and maturation of oocytes is TCM-199, and 1 to 20% serum supplement including fetal calf serum, newborn serum, estrual cow serum, lamb serum 15 or steer serum. A preferred maintenance medium includes TCM- 199 with Earl salts, 10% fetal calf serum, 0.2 MM Ma pyruvate and 50 pg/ml gentamicin sulphate. Any of the above may also involve co-culture with a variety of cell types such as granulosa cells, oviduct cells, BRL cells and uterine cells and STO cells. In particular, human epithelial cells of the endometrium secrete leukemia 20 inhibitory factor (LIF) during the preimplantation and implantation period. Therefore, the addition of LIF to the culture medium could be of importance in enhancing the in vitro development of the reconstructed embryos. The use of LIF for embronic or stem like cell cultures has been described in U.S. Patent 5,712,156, which is herein incorporated by reference. 25 Another maintenance medium is described in U.S. Patent 5,096,822 to Rosenkrans, Jr. et al., which is incorporated herein by reference. This embryo medium, named CR1, contains the nutritional substances necessary to support an embryo. CR1 contains hemicalcium L-lactate in amounts ranging from 1.0 mM to 10 mM, preferably WO 00/52145 PCT/USOO/05434 -22 1.0 mM to 5.0 mM. Hemicalcium L-lactate is L-lactate with a hemicalcium salt incorporated thereon. Also, suitable culture medium for maintaining human embryonic cells in culture as discussed in Thomson et al., Science 282:1145-1147 (1998) and Proc. Natl. Acad. 5 Sci., USA 92:7844-7848 (1995). Afterward, the cultured NT unit or units are preferably washed and then placed in a suitable media, e.g., CRIaa medium, Ham's F-10, Tissue Culture Media -199 (TCM-199). Tyrodes-Albumin-Lactate-Pyrovate (TALP) Dulbecco's Phosphate Buffered Saline (PBS), Eagle's or Whitten's, preferably containing about 10% FCS. 10 Such culturing will preferably be effected in well plates which contain a suitable conflu ent feeder layer. Suitable feeder layers include, by way of example, fibroblasts and epithelial cells, e.g., fibroblasts and uterine epithelial cells derived from ungulates, chicken fibroblasts, murine (e.g., mouse or rat) fibroblasts, STO and SI-m220 feeder cell lines, and BRL cells. 15 In the preferred embodiment, the feeder cells will comprise mouse embryonic fibroblasts. Means for preparation of a suitable fibroblast feeder layer are described in the example which follows and is well within the skill of the ordinary artisan. The NT units are cultured on the feeder layer until the NT units reach a size suitable for obtaining cells which may be used to produce embryonic stem-like cells or 20 cell colonies. Preferably, these NT units will be cultured until they reach a size of at least about 2 to 400 cells, more preferably about 4 to 128 cells, and most preferably at least about 50 cells. The culturing will be effected under suitable conditions, i.e., about 38.5 C and 5% CO 2 , with the culture medium changed in order to optimize growth typically about every 2-5 days, preferably about every 3 days. 25 In the case of human cell/enucleated bovine oocyte derived NT units, sufficient cells to produce an ES cell colony, typically on the order of about 50 cells, will be obtained about 12 days after initiation of oocyte activation. However, this may vary dependent upon the particular cell used as the nuclear donor, the species of the WO 00/52145 PCT/USOO/05434 - 23 particular oocyte, and culturing conditions. One skilled in the art can readily ascertain visually when a desired sufficient number of cells has been obtained based on the morphology of the cultured NT units. In the case of human/human nuclear transfer embryos, it may be advantageous 5 to use culture medium known to be useful for maintaining human cells in tissue culture. Examples of a culture media suitable for human embryo culture include the medium reported in Jones et al, Human Reprod., 13(l):169-177 (1998), the P1-catalog #99242 medium, and the P-1 catalog #99292 medium, both available from Irvine Scientific, Santa Ana, California, and those used by Thomson et al. (1998) and (1995). (Id.). 10 As discussed above, the cells used in the present invention will preferably comprise mammalian somatic cells, most preferably cells derived from an actively proliferating (non-quiescent) mammalian cell culture. In an especially preferred embodiment, the donor cell will be genetically modified by the addition, deletion or substitution of a desired DNA sequence. For example, the donor cell, e.g., a 15 keratinocyte or fibroblast, e.g., of human, primate or bovine origin, may be transfected or transformed with a DNA construct that provides for the expression of a desired gene product, e.g., therapeutic polypeptide. Examples thereof include lympokines, e.g., IGF I, IGF-II, interferons, colony stimulating factors, connective tissue polypeptides such as collagens, genetic factors, clotting factors, enzymes, enzyme inhibitors, etc. 20 Also, as discussed above, the donor cells may be modified prior to nuclear transfer to achieve other desired effects, e.g., impaired cell lineage development, enhanced embryonic development and/or inhibition of apoptosis. Examples of desirable modifications are discussed further below. One aspect of the invention will involve genetic modification of the donor cell, 25 e.g., a human cell, such that it is lineage deficient and therefore when used for nuclear transfer it will be unable to give rise to a viable offspring. This is desirable especially in the context of human nuclear transfer embryos, wherein for ethical reasons, production of a viable embryo may be an unwanted outcome. This can be effected by WO 00/52145 PCTUSOO/05434 - 24 genetically engineering a human cell such that it is incapable of differentiating into specific cell lineages when used for nuclear transfer. In particular, cells may be genetically modified such that when used as nuclear transfer donors the resultant "embryos" do not contain or substantially lack at least one of mesoderm, endoderm or 5 ectoderm tissue. It is anticipated that this can be accomplished by knocking out or impairing the expression of one or more mesoderm, endoderm or ectoderm specific genes. Examples thereof include: Mesdoerm: SRF, MESP-1, HNF-4, beta-I integrin, MSD; 10 Endoderm: GATA-6, GATA-4; Ectoderm: RNA helicase A, H beta 58. The above list is intended to be exemplary and non-exhaustive of known genes which are involved in the development of mesoderm, endoderm and ectoderm. The generation of mesoderm deficient, endoderm deficient and ectoderm deficient cells and 15 embryos has been previously reported in the literature. See, e.g., Arsenian et al, EMBO J., Vol. 17(2):6289-6299 (1998); Saga Y, Mech. Dev., Vol. 75(1-2):53-66 (1998); Holdener et al, Development, Voll 120(5):1355-1346 (1994); Chen et al, Genes Dev. Vol. 8(20):2466-2477 (1994); Rohwedel et al, Dev. Biol., 201(2):167-189 (1998) (mesoderm); Morrisey et al, Genes, Dev., Vol. 12(22):3579-3590 (1998); Soudais et al, 20 Development, Vol. 121(11):3877-3888 (1995) (endoderm); and Lee et al, Proc. Natl. Acad. Sci. USA, Vol. 95:(23):13709-13713 (1998); and Radice et al, Development, Vol. 111(3):801-811 (1991) (ectoderm). In general, a desired somatic cell, e.g., a human keratinocyte, epithelial cell or fibroblast, will be genetically engineered such that one or more genes specific to 25 particular cell lineages are "knocked out" and/or the expression of such genes significantly impaired. This may be effected by known methods, e.g., homologous recombination. A preferred genetic system for effecting "knock-out" of desired genes is disclosed by Capecchi et al, U.S. Patents 5,631,153 and 5,464,764, which reports WO 00/52145 PCTIUSOO/05434 - 25 positive-negative selection (PNS) vectors that enable targeted modification of DNA sequences in a desired mammalian genome. Such genetic modification will result in a cell that is incapable of differentiating into a particular cell lineage when used as a nuclear transfer donor. 5 This genetically modified cell will be used to produce a lineage-defective nuclear transfer embryo, i.e., that does not develop at least one of a functional mesoderm, endoderm or ectoderm. Thereby, the resultant embryos, even if implanted, e.g., into a human uterus, would not give rise to a viable offspring. However, the ES cells that result from such nuclear transfer will still be useful in that they will produce 10 cells of the one or two remaining non-impaired lineage. For example, an ectoderm deficient human nuclear transfer embryo will still give rise to mesoderm and endoderm derived differentiated cells. An ectoderm deficient cell can be produced by deletion and/or impairment of one or both of RNA helicase A or H beta 58 genes. These lineage deficient donor cells may also be genetically modified to express 15 another desired DNA sequence. Thus, the genetically modified donor cell will give rise to a lineage-deficient blastocyst which, when plated, will differentiate into at most two of the embryonic germ layers. Alternatively, the donor cell can be modified such that it is "mortal". This can 20 be achieved by expressing anti-sense or ribozyme telomerase genes. This can be effected by known genetic methods that will provide for expression of antisense DNA or ribozymes, or by gene knockout. These "mortal" cells, when used for nuclear transfer, will not be capable of differentiating into viable offspring. Another preferred embodiment of the present invention is the production of 25 nuclear transfer embryos that grow more efficiently in tissue culture. This is advantageous in that it should reduce the requisite time and necessary fusions to produce ES cells and/or offspring (if the blastocysts are to be implanted into a female surrogate). This is desirable also because it has been observed that blastocysts and ES WO 00/52145 PCT/USOO/05434 -26 cells resulting from nuclear transfer may have impaired development potential. While these problems may often be alleviated by alteration of tissue culture conditions, an alternative solution is to enhance embryonic development by enhancing expression of genes involved in embryonic development. 5 For example, it has been reported that the gene products of the Ped type, which are members of the MHC I family, are of significant importance to embryonic development. More specifically, it has been reported in the case of mouse preimplantation embryos that the Q7 and Q9 genes are responsible for the "fast growth" phenotype. Therefore, it is anticipated that introduction of DNAs that provide for the 10 expression of these and related genes, or their human or other mammalian counterparts into donor cells, will give rise to nuclear transfer embryos that grow more quickly. This is particularly desirable in the context of cross-species nuclear transfer embryos which may develop less efficiently in tissue culture than nuclear transfer embryos produced by fusion of cells or nuclei of the same species. 15 In particular, a DNA construct containing the Q7 and/or Q9 gene will be introduced into donor somatic cells prior to nuclear transfer. For example, an expression construct can be constructed containing a strong constitutive mammalian promoter operably linked to the Q7 and/or Q9 genes, an IRES, one or more suitable selectable markers, e.g,. neomycin, ADA, DHFR, and a poly-A sequence, e.g., bGH 20 polyA sequence. Also, it may be advantageous to further enhance Q7 and Q9 gene expression by the inclusion of insulates. It is anticipated that these genes will be expressed early on in blastocyst development as these genes are highly conserved in different species, e.g., bovines, goats, porcine, dogs, cats, and humans. Also, it is anticipated that donor cells can be engineered to affect other genes that enhance 25 embryonic development. Thus, these genetically modified donor cells should produce blastocysts and preimplantation stage embryos more efficiently. Still another aspect of the invention involves the construction of donor cells that are resistant to apoptosis, i.e., programmed cell death. It has been reported in the WO 00/52145 PCTIUSOO/05434 - 27 literature that cell death related genes are present in preimplantation stage embryos. (Adams et al, Science, 281(5381):1322-1326 (1998)). Genes reported to induce apoptosis include, e.g., Bad, Bok, BH3, Bik, Hrk, BNIP3, BimL, Bad, Bid, and EGL-1. By contrast, genes that reportedly protect cells from programmed cell death include, by 5 way of example, BcL-XL, Bcl-w, Mcl-1, Al, Nr-13, BHRF-1, LMW5-HL, ORF16, Ks-Bel-2, E1B-19K, and CED-9. Thus, donor cells can be constructed wherein genes that induce apoptosis are "knocked out" or wherein the expression of genes that protect the cells from apoptosis is enhanced or turned on during embryonic development. 10 For example, this can be effected by introducing a DNA construct that provides for regulated expression of such protective genes, e.g., Bcl-2 or related genes during embryonic development. Thereby, the gene can be "turned on" by culturing the embryo under specific growth conditions. Alternatively, it can be linked to a constitutive promoter. 15 More specifically, a DNA construct containing a Bcl-2 gene operably linked to a regulatable or constitutive promoter, e.g., PGK, SV40, CMV, ubiquitin, or beta-actin, an IRES, a suitable selectable marker, and a poly-A sequence can be constructed and introduced into a desired donor mammalian cell, e.g., human keratinocyte or fibroblast. These donor cells, when used to produce nuclear transfer embryos, should be 20 resistant to apoptosis and thereby differentiate more efficiently in tissue culture. Thereby, the speed and/or number of suitable preimplantation embryos produced by nuclear transfer can be increased. Another means of accomplishing the same result is to impair the expression of one or more genes that induce apoptosis. This will be effected by knock-out or by the 25 use of antisense or ribozymes against genes that are expressed in and which induce apoptosis early on in embryonic development. Examples thereof are identified above. Still alternatively, donor cells may be constructed containing both modifications, i.e., WO 00/52145 PCT/USOO/05434 -28 impairment of apoptosis-inducing genes and enhanced expression of genes that impede or prevent apoptosis. The construction and selection of genes that affect apoptosis, and cell lines that express such genes, is disclosed in U.S. Patent No. 5,646,008, Craig B. Thompson et al inventors, and assigned to the University of Michigan. This patent is 5 incorporated by reference herein. One means of enhancing efficiency is to select cells of a particular cell cycle stage as the donor cell. It has been reported that this can have significant effects on nuclear transfer efficiency. (Barnes et al, Mol. Reprod. Devel., 36(1):33-41 (1993). Different methods for selecting cells of a particular cell cycle stage have been reported 10 and include serum starvation (Campbell et al, Nature, 380:64-66 (1996); Wilmut et al, Nature, 385:810-813 (1997), and chemical synchronization (Urbani et al, Exp. Cell Res., 219(l):159-168 (1995). For example, a particular cyclin DNA may be operably linked to a regulatory sequence, together with a detectable marker, e.g., green fluorescent protein (GFP), followed by the cyclin destruction box, and optionally 15 insulation sequences to enhance cyclin and marker protein expression. Thereby, cells of a desired cell cycle can be easily visually detected and selected for use as a nuclear transfer donor. An example thereof is the cyclin D1 gene in order to select for cells that are in G1. However, any cyclin gene should be suitable for use in the claimed invention. (See, e.g., King et al, Mol. Bio. Cell, Vol. 7(9):1343-1357 (1996)). 20 However, a less invasive or more efficient method for producing cells of a desired cell cycle stage are needed. It is anticipated that this can be effected by genetically modifying donor cells such that they express specific cyclins under detectable conditions. Thereby, cells of a specific cell cycle can be readily discerned from other cell cycles. 25 Cyclins are proteins that are expressed only during specific stages of the cell cycle. They include cyclin Dl, D2 and D3 in G1 phase, cyclin BI and B2 in G2/M phase and cyclin E, A and H in S phase. These proteins are easily translated and destroyed in the cytogolcytosol. This "transient" expression of such proteins is WO 00/52145 PCT/US0O/05434 - 29 attributable in part to the presence of a "destruction box", which is a short amino acid sequence that is part of the protein that functions as a tag to direct the prompt destruction of these proteins via the ugiquitin pathway. (Adams et al, Science, 281 (5321):1322-1326 (1998)). 5 In the present invention, donor cells will be constructed that express one or more of such cyclin genes under easily detectable conditions, preferably visualizable, e.g., by the use of a fluorescent label. For example, a particular cyclin DNA may be operably linked to a regulatory sequence, together with a detectable marker, e.g., green fluorescent protein (GFP), followed by the cyclin destruction box, and optionally 10 insulation sequences to enhance cyclin and/or marker protein expression. Thereby, cells of a desired cell cycle can be easily visually detected and selected for use as a nuclear transfer donor. An example thereof is the cyclin Dl gene which can be used to select for cells that are in G1. However, any cyclin gene should be suitable for use in the claimed invention. (See, e.g., King et al, Mol. Bio. Cell, Vol. 7(9):1343-1357 15 (1996)). Still another aspect of the invention is a method for enhancing nuclear transfer efficiency, preferably in a cross-species nuclear transfer process. While the present inventors have demonstrated that nuclei or cells of one species when inserted or fused with an enucleated oocyte of a different species can give rise to nuclear transfer 20 embryos that produce blastocysts, which embryos can give rise to ES cell lines, the efficiency of such process is quite low. Therefore, many fusions typically need to be effected to produce a blastocyst the cells of which may be cultured to produce ES cells and ES cell lines. Yet another means for enhancing the development of nuclear transfer embryos 25 in vitro is by optimizing culture conditions. One means of achieving this result will be to culture NT embryos under conditions impede apoptosis. With respect to this embodiment of the invention, it has been found that proteases such as capsases can WO 00/52145 PCT/USOO/05434 -30 cause oocyte death by apoptosis similar to other cell types. (See, Jurisicosva et al, Mol. Reprod. Devel., 51(3):243-253 (1998).) It is anticipated that blastocyst development will be enhanced by including in culture media used for nuclear transfer and to maintain blastocysts or culture 5 preimplantation stage embryos one or more capsase inhibitors. Such inhibitors include by way of example capsase-4 inhibitor I, capsase-3 inhibitor I, capsase-6 inhibitor II, capsase-9 inhibitor II, and capsase-1 inhibitor I. the amount thereof will be an amount effective to inhibit apoptosis, e.g., 0.00001 to 5.0% by weight of medium; more preferably 0.01% to 1.0% by weight of medium. Thus, the foregoing methods may be 10 used to increase the efficiency of nuclear transfer by enhancing subsequent blastocyst and embryo development in tissue culture. After NT units of the desired size are obtained, the cells are mechanically re moved from the zone and are then used to produce embryonic or stem-like cells and cell lines. This is preferably effected by taking the clump of cells which comprise the NT 15 unit, which typically will contain at least about 50 cells, washing such cells, and plating the cells onto a feeder layer, e.g., irradiated fibroblast cells. Typically, the cells used to obtain the stem-like cells or cell colonies will be obtained from the inner most portion of the cultured NT unit which is preferably at least 50 cells in size. However, NT units of smaller or greater cell numbers as well as cells from other portions of the 20 NT unit may also be used to obtain ES-like cells and cell colonies. It may be that a longer exposure of donor cell DNA to the oocyte's cytosol would facilitate the dedifferentiation process. In this regard, recloning could be accom plished by taking blastomeres from a reconstructed embryo and fusing them with a new enucleated oocyte. Alternatively, the donor cell may be fused with an enucleated 25 oocyte and 4 to 6 hours later, without activation, chromosomes may be removed and fused with a younger oocyte. Activation would occur thereafter. The cells are maintained in the feeder layer in a suitable growth medium, e.g., alpha MEM supplemented with 10% FCS and 0.1 mM beta-mercaptoethanol (Sigma) WO 00/52145 PCTIUSOO/05434 -31 and L-glutamine. The growth medium is changed as often as necessary to optimize growth, e.g., about every 2-3 days. This culturing process results in the formation of embryonic or stem-like cells or cell lines. In the case of human cell/bovine oocyte derived NT embryos, colonies are 5 observed by about the second day of culturing in the alpha MEM medium. However, this time may vary dependent upon the particular nuclear donor cell, specific oocyte and culturing conditions. One skilled in the art can vary the culturing conditions as desired to optimize growth of the particular embryonic or stem-like cells. The embryonic or stem-like cells and cell colonies obtained will typically 10 exhibit an appearance similar to embryonic or stem-like cells of the species used as the nuclear cell donor rather than the species of the donor oocyte. For example, in the case of embryonic or stem-like cells obtained by the transfer of a human nuclear donor cell into an enucleated bovine oocyte, the cells exhibit a morphology more similar to mouse embryonic stem cells than bovine ES-like cells. 15 More specifically, the individual cells of the human ES-line cell colony are not well defined, and the perimeter of the colony is refractive and smooth in appearance. Further, the cell colony has a longer cell doubling time, about twice that of mouse ES cells. Also, unlike bovine and porcine derived ES cells, the colony does not possess an epithelial-like appearance. 20 As discussed above, it has been reported by Thomson, in U.S. Patent 5,843,780, that primate stem cells are SSEA-1 (-), SSEA-4 (+), TRA-1-60 (+), TRA-1-81 (+) and alkaline phosphatase (+). It is anticipated that human and primate ES cells produced according to the present methods will exhibit similar or identical marker expression. Alternatively, that such cells are actual human or primate embryonic stem cells 25 will be confirmed based on their capability of giving rise to all of mesoderm, ectoderm and endoderm tissues. This will be demonstrated by culturing ES cells produced according to the invention under appropriate conditions, e.g., as disclosed by Thomsen, U.S. Patent 5,843,780, incorporated by reference in its entirety herein.

WO 00/52145 PCTIUSOO/05434 - 32 The resultant embryonic or stem-like cells and cell lines, preferably human embryonic or stem-like cells and cell lines, have numerous therapeutic and diagnostic applications. Most especially, such embryonic or stem-like cells may be used for cell transplantation therapies. Human embryonic or stem-like cells have application in the 5 treatment of numerous disease conditions. In this regard, it is known that mouse embryonic stem (ES) cells are capable of differentiating into almost any cell type, e.g., hematopoietic stem cells. Therefore, human embryonic or stem-like cells produced according to the invention should possess similar differentiation capacity. The embryonic or stem-like cells according to the 10 invention will be induced to differentiate to obtain the desired cell types according to known methods. For example, the subject human embryonic or stem-like cells may be induced to differentiate into hematopoietic stem cells, muscle cells, cardiac muscle cells, liver cells, cartilage cells, epithelial cells, urinary tract cells, etc., by culturing such cells in differentiation medium and under conditions which provide for cell 15 differentiation. Medium and methods which result in the differentiation of embryonic stem cells are known in the art as are suitable culturing conditions. For example, Palacios et al, Proc. NatL. Acad. Sci., USA, 92:7530-7537 (1995) teaches the production of hematopoietic stem cells from an embryonic cell line by subjecting stem cells to an induction procedure comprising initially culturing aggregates 20 of such cells in a suspension culture medium lacking retinoic acid followed by culturing in the same medium containing retinoic acid, followed by transferral of cell aggregates to a substrate which provides for cell attachment. Moreover, Pedersen, J Reprod. Fertil. Dev., 6:543-552 (1994) is a review article which references numerous articles disclosing methods for in vitro 25 differentiation of embryonic stem cells to produce various differentiated cell types including hematopoietic cells, muscle, cardiac muscle, nerve cells, among others. Further, Bain et al, Dev. Biol., 168:342-357 (1995) teaches in vitro differentiation of embryonic stem cells to produce neural cells which possess neuronal WO 00/52145 PCTIUSOO/05434 - 33 properties. These references are exemplary of reported methods for obtaining differentiated cells from embryonic or stem-like cells. These references and in particular the disclosures therein relating to methods for differentiating embryonic stem cells are incorporated by reference in their entirety herein. 5 Thus, using known methods and culture medium, one skilled in the art may culture the subject embryonic or stem-like cells to obtain desired differentiated cell types, e.g., neural cells, muscle cells, hematopoietic cells, etc. In addition, the use of inducible Bcl-2 or Bcl-xl might be useful for enhancing in vitro development of specific cell lineages. In vivo, Bcl-2 prevents many, but not all, forms of apoptotic cell 10 death that occur during lymphoid and neural development. A thorough discussion of how Bcl-2 expression might be used to inhibit apoptosis of relevent cell lineages following transfection of donor cells is disclosed in U.S. Patent No. 5,646,008, which is herein incorporated by reference. The subject embryonic or stem-like cells may be used to obtain any desired 15 differentiated cell type. Therapeutic usages of such differentiated human cells are unparalleled. For example, human hematopoietic stem cells may be used in medical treatments requiring bone marrow transplantation. Such procedures are used to treat many diseases, e.g., late stage cancers such as ovarian cancer and leukemia, as well as diseases that compromise the immune system, such as AIDS. Hematopoietic stem cells 20 can be obtained, e.g., by fusing adult somatic cells of a cancer or AIDS patient, e.g., epithelial cells or lymphocytes with an enucleated oocyte, e.g., bovine oocyte, obtaining embryonic or stem-like cells as described above, and culturing such cells under condi tions which favor differentiation, until hematopoietic stem cells are obtained. Such hematopoietic cells may be used in the treatment of diseases including cancer and 25 AIDS. Alternatively, adult somatic cells from a patient with a neurological disorder may be fused with an enucleated animal oocyte, e.g., a primate or bovine oocyte, human embryonic or stem-like cells obtained therefrom, and such cells cultured under WO 00/52145 PCT/USOO/05434 - 34 differentiation conditions to produce neural cell lines. Specific diseases treatable by transplantation of such human neural cells include, by way of example, Parkinson's disease, Alzheimer's disease, ALS and cerebral palsy, among others. In the specific case of Parkinson's disease, it has been demonstrated that transplanted fetal brain neural 5 cells make the proper connections with surrounding cells and produce dopamine. This can result in long-term reversal of Parkinson's disease symptoms. To allow for specific selection of differentiated cells, donor cells may be transfected with selectable markers expressed via inducible promoters, thereby permit ting selection or enrichment of particular cell lineages when differentiation is induced. 10 For example, CD34-neo may be used for selection of hematopoietic cells, Pwl -neo for muscle cells, Mash-1-neo for sympathetic neurons, Mal-neo for human CNS heurons of the grey matter of the cerebral cortex, etc. The great advantage of the subject invention is that it provides an essentially limitless supply of isogenic or synegenic human cells suitable for transplantation. 15 Therefore, it will obviate the significant problem associated with current transplantation methods, i.e., rejection of the transplanted tissue which may occur because of host-vs graft or graft-vs-host rejection. Conventionally, rejection is prevented or reduced by the administration of anti-rejection drugs such as cyclosporine. However, such drugs have significant adverse side-effects, e.g., immunosuppression, carcinogenic properties, 20 as well as being very expensive. The present invention should eliminate, or at least greatly reduce, the need for anti-rejection drugs. Other diseases and conditions treatable by isogenic cell therapy include, by way of example, spinal cord injuries, multiple sclerosis, muscular dystrophy, diabetes, liver diseases, i.e., hypercholesterolemia, heart diseases, cartilage replacement, bums, foot 25 ulcers, gastrointestinal diseases, vascular diseases, kidney disease, urinary tract disease, and aging related diseases and conditions. Also, human embryonic or stem-like cells produced according to the invention may be used to produce genetically engineered or transgenic human differentiated cells.

* WO 00/52145 PCT/USOO/05434 - 35 Essentially, this will be effected by introducing a desired gene or genes, which may be heterologous, or removing all or part of an endogenous gene or genes of human embryonic or stem-like cells produced according to the invention, and allowing such cells to differentiate into the desired cell type. A preferred method for achieving such 5 modification is by homologous recombination because such technique can be used to insert, delete or modify a gene or genes at a specific cite or cites in the stem-like cell genome. This methodology can be used to replace defective genes, e.g., defective immune system genes, cystic fibrosis genes, or to introduce genes which result in the 10 expression of therapeutically beneficial proteins such as growth factors, lymphokines, cytokines, enzymes, etc. For example, the gene encoding brain derived growth factor may be introduced into human embryonic or stem-like cells, the cells differentiated into neural cells and the cells transplanted into a Parkinson's patient to retard the loss of neural cells during such disease. 15 Previously, cell types transfected with BDNF varied from primary cells to immortalized cell lines, either neural or non-neural (myoblast and fibroblast) derived cells. For example, astrocytes have been transfected with BDNF gene using retroviral vectors, and the cells grafted into a rat model of Parkinson's disease (Yoshimoto et al., Brain Research, 691:25-36, (1995)). 20 This ex vivo therapy reduced Parkinson's-like symptoms in the rats up to 45% 32 days after transfer. Also, the tyrosine hydroxylase gene has been placed into astro cytes with similar results (Lundberg et al., Develop. Neurol., 139:39-53 (1996) and references cited therein). However, such ex vivo systems have problems. In particular, retroviral vectors 25 currently used are down-regulated in vivo and the transgene is only transiently expressed (review by Mulligan, Science, 260:926-932 (1993)). Also, such studies used primary cells, astrocytes, which have finite life span and replicate slowly. Such properties adversely affect the rate of transfection and impede selection of stably WO 00/52145 PCT/USOO/05434 - 36 transfected cells. Moreover, it is almost impossible to propagate a large population of gene targeted primary cells to be used in homologous recombination techniques. By contrast, the difficulties associated with retroviral systems should be eliminated by the use of human embryonic or stem-like cells. It has been demonstrated 5 previously by the subject assignee that cattle and pig embryonic cell lines can be transfected and selected for stable integration of heterologous DNA. Such methods are described in commonly assigned U.S. Serial No. 08/626,054, filed April 1, 1996, incorporated by reference in its entirety. Therefore, using such methods or other known methods, desired genes may be introduced into the subject human embryonic or stem 10 like cells, and the cells differentiated into desired cell types, e.g., hematopoietic cells, neural cells, pancreatic cells, cartilage cells, etc. Genes which may be introduced into the subject embryonic or stem-like cells include, by way of example, epidermal growth factor, basic fibroblast growth factor, glial derived neurotrophic growth factor, insulin-like growth factor (I and II), 15 neurotrophin-3, neurotrophin-4/5, ciliary neurotrophic factor, AFT-1, cytokine genes (interleukins, interferons, colony stimulating factors, tumor necrosis factors (alpha and beta), etc.), genes encoding therapeutic enzymes, collagen, human serum albumin, etc. In addition, it is also possible to use one of the negative selection systems now 20 known in the art for eliminating therapeutic cells from a patient if necessary. For example, donor cells transfected with the thymidine kinase (TK) gene will lead to the production of embryonic cells containing the TK gene. Differentiation of these cells will lead to the isolation of therapeutic cells of interest which also express the TK gene. Such cells may be selectively eliminated at any time from a patient upon gancyclovir 25 administration. Such a negative selection system is described in U.S. Patent No. 5,698,446, and is herein incorporated by reference.

WO 00/52145 PCTIUSOO/05434 - 37 The subject embryonic or stem-like cells, preferably human cells, also may be used as an in vitro model of differentiation, in particular for the study of genes which are involved in the regulation of early development. Also, differentiated cell tissues and organs using the subject embryonic or stem 5 like cells may be used in drug studies. Further, the subject embryonic or stem-like cells may be used as nuclear donors for the production of other embryonic or stem-like cells and cell colonies. In order to more clearly describe the subject invention, the following examples are provided. 10 EXAMPLE 1 MATERIALS AND METHODS Donor Cells for Nuclear Transfer Epithelial cells were lightly scraped from the inside of the mouth of a consenting adult with a standard glass slide. The cells were washed off the slide into 15 a petri dish containing phosphate buffered saline without Ca or Mg. The cells were pipetted through a small-bore pipette to break up cell clumps into a single cell suspension. The cells were then transferred into a microdrop of TL-HEPES medium containing 10% fetal calf serum (FCS) under oil for nuclear transfer into enucleated cattle oocytes. 20 Nuclear Transfer Procedures Basic nuclear transfer procedures have been described previously. Briefly, after slaughterhouse oocytes were matured in vitro the oocytes were stripped of cumulus cells and enucleated with a beveled micropipette at approximately 18 hours post maturation (hpm). Enucleation was confirmed in TL-HEPES medium plus 25 bisbenzimide (Hoechst 33342, 3 gg/ml; Sigma). Individual donor cells were then placed into the perivitelline space of the recipient oocyte. The bovine oocyte cytoplasm and the donor nucleus (NT unit) are fused together using electrofusion techniques. One fusion pulse consisting of 90 V for 15 gsec was applied to the NT unit. This occurred WO 00/52145 PCTUSOO/05434 - 38 at 24 hours post-initiation of maturation (hpm) of the oocytes. The NT units were placed in CRlaa medium until 28 hpm. The procedure used to artificially activate oocytes has been described elsewhere. NT unit activation was at 28 hpm. A brief description of the activation procedure is as 5 follows: NT units were exposed for four min to ionomycin (5 gM; CalBiochem, La Jolla, CA) in TL-HEPES supplemented with 1 mg/ml BSA and then washed for five min in TL-HEPES supplemented with 30 mg/ml BSA. The NT units were then transferred into a microdrop of CR1aa culture medium containing 0.2 mM DMAP (Sigma) and cultured at 38.5 C 5% CO 2 for four to five hours. The NT units were 10 washed and then placed in a CR1aa medium plus 10% FCS and 6 mg/ml BSA in four well plates containing a confluent feeder layer of mouse embryonic fibroblasts (described below). The NT units were cultured for three more days at 38.5 C and 5%

CO

2 . The culture medium was changed every three days until day 12 after the time of activation. At this time NT units reaching the desired cell number, i.e., about 50 cell 15 number, were mechanically removed from the zona and used to produce embryonic cell lines. A photograph of an NT unit obtained as described above is contained in Figure 1. Fibroblast feeder layer Primary cultures of embryonic fibroblasts were obtained from 14-16 day old 20 murine fetuses. After the head, liver, heart and alimentary tract were aseptically re moved, the embryos were minced and incubated for 30 minutes at 37'C in prewarmed trypsin EDTA solution (0.05% trypsin/0.02% EDTA; GIBCO, Grand Island, NY). Fibroblast cells were plated in tissue culture flasks and cultured in alpha-MEM medium (BioWhittaker, Walkersville, MD) supplemented with 10% fetal calf serum (FCS) 25 (Hyclone, Logen, UT), penicillin (100 IU/ml) and streptomycin (50 il/ml). Three to four days after passage, embryonic fibroblasts, in 35 x 10 Nunc culture dishes (Baxter Scientific, McGaw Park, IL), were irradiated. The irradiated fibroblasts were grown and maintained in a humidified atmosphere with 5% CO 2 in air at 37 C. The culture WO 00/52145 PCT/USOO/05434 - 39 plates which had a uniform monolayer of cells were then used to culture embryonic cell lines. Production of embryonic cell line. NT unit cells obtained as described above were washed and plated directly onto 5 irradiated feeder fibroblast cells. These cells included those of the inner portion of the NT unit. The cells were maintained in a growth medium consisting of alpha MEM supplemented with 10% FCS and 0.1 mM beta-mercaptoethanol (Sigma). Growth medium was exchanged every two to three days. The initial colony was observed by the second day of culture. The colony was propagated and exhibits a similar mor 10 phology to previously disclosed mouse embryonic stem (ES) cells. Individual cells within the colony are not well defined and the perimeter of the colony is refractile and smooth in appearance. The cell colony appears to have a slower cell doubling time than mouse ES cells. Also, unlike bovine and porcine derived ES cells, the colony does not have an epithelial appearance thus far. Figures 2 through 5 are photographs of ES-like 15 cell colonies obtained as described, supra. Production of Differentiated Human Cells The human embryonic cells obtained are transferred to a differentiation medium and cultured until differentiated human cell types are obtained. RESULTS 20 Table 1. Human cells as donor nuclei in NT unit production and development.

WO 00/52145 PCT/USOO/05434 - 40 TABLE 1 Cell type No. NT No. NT units No. NT units to 4 No. NT units to units made 2 cell stage - 16 cell stage (%) 16 - 400 cell (%) stage (%) lymphocytes 18 12 (67%) 3 (17%) 0 5 epilhevm34 18 (53%) 3 (9%) 1(3%) adult fibro- 12 (4 cell; 26%) blasts 46 4 (9%) 8 (8-16 cells; -- 1-_ 1 17.4%) The one NT unit that developed a structure having greater than 16 cells was plated down onto a fibroblast feeder layer. This structure was attached to 10 the feeder layer and started to propagate forming a colony with a ES cell-like morphology (See, e.g., Figure 2). Moreover, although the 4 to 16 cell stage structures were not used to try and produce an ES cell colony, it has been previ ously shown that this stage is capable of producing ES or ES-like cell lines (mouse, Eistetter et al., Devel. Growth and Differ., 31:275-282 (1989); Bovine, 15 Stice et al., 1996)). Therefore, it is expected that 4 - 16 cell stage NT units should also give rise to embryonic or stem-like cells and cell colonies. Also, similar results were obtained upon fusion of an adult human keratinocyte cell line with an enucleated bovine oocyte, which was cultured in media comprising ACM, uridine, glucose, and 1000 IU of LIF. Out of 50 20 reconstructed embryos, 22 cleaved and one developed into a blastocyst at about day 12. This blastocyst was plated and the production of an ES cell line is ongoing. While the present invention has been described and illustrated herein by reference to various specific materials, procedures, and examples, it is under 25 stood that the invention is not restricted to the particular material, combinations WO 60/52145 PCT/USOO/05434 -41 of materials, and procedures selected for that purpose. Numerous variations of such details can be implied and will be appreciated by those skilled in the art.

Claims

1. A method of producing embryonic or stem-like cells comprising the following steps: (i) inserting a desired differentiated human or mammalian cell 5 or cell nucleus into an enucleated animal oocyte, wherein such oocyte is derived from a different animal species than the human or mammalian cell under condi tions suitable for the formation of a nuclear transfer (NT) unit; (ii) activating the resultant nuclear transfer unit; (iii) culturing said activated nuclear transfer unit until greater 10 than the 2-cell developmental stage; and (iv) culturing cells obtained from said cultured NT units to obtain embryonic or stem-like cells.

2. The method of Claim 1, wherein the cell inserted into the enucleat 15 ed animal oocyte is a human cell.

3. The method of Claim 2, wherein said human cell is an adult cell.

4. The method of Claim 2, wherein said human cell is an epithelial cell, keratinocyte, lymphocyte or fibroblast.

5. The method of Claim 2, wherein the oocytes are obtained from a 2 0 mammal. WO 00/52145 PCTIUSOO/05434 - 43

6. The method of Claim 5, wherein the animal oocyte is obtained from an ungulate.

7. The method of Claim 6, wherein said ungulate is selected from the group consisting of bovine, ovine, porcine, equine, capine, and buffalo. 5

8. The method of Claim 1, wherein the enucleated oocyte is matured prior to enucleation.

9. The method of Claim 1, wherein the fused nuclear transfer units are activated in vitro.

10. The method of Claim 1, wherein the activated nuclear transfer units 10 are cultured on a feeder layer culture.

11. The method of Claim 10, wherein the feeder layer comprises fibro blasts.

12. The method of Claim 1, wherein in step (iv) cells from a NT unit having 16 cells or more are cultured on a feeder cell layer. 15

13. The method of Claim 12, wherein said feeder cell layer comprises fibroblasts.

14. The method of Claim 13, wherein said fibroblasts comprise mouse embryonic fibroblasts. WO 00/52145 PCTUSOO/05434 -44

15. The method of Claim 1, wherein the resultant embryonic or stem like cells are induced to differentiate.

16. The method of Claim 2, wherein the resultant embryonic or stem like cells are induced to differentiate. 5

17. The method of Claim 1, wherein fusion is effected by electrofu Sion.

18. Embryonic or stem-like cells obtained according to the method of Claim 1.

19. Human embryonic or stem-like cells obtained according to the 10 method of Claim 2.

20. Human embryonic or stem-like cells obtained according to the method of Claim 3.

21. Human embryonic or stem-like cells obtained according to the method of Claim 4. 15

22. Human embryonic or stem-like cells obtained according to the method of Claim 6.

23. Human embryonic or stem-like cells obtained according to the method of Claim 7. WO 00/52145 PCT/USOO/05434 -45

24. Differentiated human cells obtained by the method of Claim 16.

25. The differentiated human cells of Claim 24, which are selected from the group consisting of neural cells, hematopoietic cells, pancreatic cells, muscle cells, cartilage cells, urinary cells, liver cells, spleen cells, reproductive 5 cells, skin cells, intestinal cells, and stomach cells.

26. A method of therapy which comprises administering to a patient in need of cell transplantation therapy isogenic differentiated human cells according to Claim 24.

27. The method of Claim 26, wherein said cell transplantation therapy 10 is effected to treat a disease or condition selected from the group consisting of Parkinson's disease, Huntington's disease, Alzheimer's disease, ALS, spinal cord defects or injuries, multiple sclerosis, muscular dystrophy, cystic fibrosis, liver disease, diabetes, heart disease, cartilage defects or injuries, burns, foot ulcers, vascular disease, urinary tract disease, AIDS and cancer. 15

28. The method of Claim 26, wherein the differentiated human cells are hematopoietic cells or neural cells.

29. The method of Claim 26, wherein the therapy is for treatment of Parkinson's disease and the differentiated cells are neural cells.

30. The method of Claim 26, wherein the therapy is for the treatment 2 0 of cancer and the differentiated cells are hematopoietic cells. WO 00/52145 PCTIUSOO/05434 - 46

31. The differentiated human cells of Claim 24, which contain and express an inserted gene.

32. The method of Claim 1, wherein a desired gene is inserted, removed or modified in said embryonic or stem-like cells. 5

33. The method of Claim 32, wherein the desired gene encodes a therapeutic enzyme, a growth factor or a cytokine.

34. The method of Claim 32, wherein said embryonic or stem-like cells are human embryonic or stem-like cells.

35. The method of Claim 32, wherein the desired gene is removed, 10 modified or deleted by homologous recombination.

36. The method of Claim 1, wherein the donor cell is genetically modified to impair the development of at least one of endoderm, ectoderm and mesoderm.

37. The method of Claim 1, wherein the donor cell is genetically 15 modified to increase differentiation efficiency.

38. The method of Claim 36, wherein the cultured nuclear transfer unit is cultured in a media containing at least one capsase inhibitor. WO 00/52145 PCTIUSOO/05434 - 47

39. The method of Claim 1, wherein the donor cell expresses a detectable label that is indicative of the expression of a particular cyclin.

40. The method of Claim 36, wherein the donor cell has been modified to alter the expression of a gene selected from the group consisting of SRF, 5 MESP-1, HNF-4, beta-1, integrin, MSD, GATA-6, GATA-4, RNA helicase A, and H beta 58.

41. The method of Claim 37, wherein said donor cell has been genetically modified to introduce a DNA that provides for expression of the Q7 and/or Q9 genes. 10

42. The method of Claim 41, wherein said gene or genes are operably linked to a regulatable promoter.

43. The method of Claim 1, wherein the donor cell has been genetically modified to inhibit apoptosis.

44. The method of Claim 43, wherein reduced apoptosis is provided 15 by altering expression of one or more genes selected from the group consisting of Bad, Bok, BH3, Bik, Blk, Hrk, BNIP3, GimL, Bid, EGL-1, Bcl-XL, Bel-w, Mcl-1, A1,Nr-13, BHRF-1, LMW5-HL, ORF16, Ks-Bcl-2, E1B-19K, and CED 9.

45. The method of Claim 44, wherein at least one of said genes is 2 0 operably linked to an inducible promoter. WO 00/52145 PCTIUSOO/05434 -48

46. A mammalian somatic cell that expresses a DNA that encodes a detectable marker, the expression of which is linked to a particular cyclin.

47. The cell of Claim 46, wherein the cyclin is selected from the group consisting of cyclin D1, D2, D3, B1, B2, E, A and H. 5

48. The cell of Claim 46, wherein the detectable marker is a fluorescent polypeptide.

49. The cell of Claim 48, wherein said mammalian cell is selected from the group consisting of human, primate, rodent, ungulate, canine, and feline cells.

50. The cell of Claim 48, wherein said cell is a human, bovine or 10 primate cell.