AU3056299A - Methods for isolating osteoclast precursor cells and inducing the differentiation of the same into osteoclasts - Google Patents

Methods for isolating osteoclast precursor cells and inducing the differentiation of the same into osteoclasts

Info

Publication number
AU3056299A
AU3056299A AU30562/99A AU3056299A AU3056299A AU 3056299 A AU3056299 A AU 3056299A AU 30562/99 A AU30562/99 A AU 30562/99A AU 3056299 A AU3056299 A AU 3056299A AU 3056299 A AU3056299 A AU 3056299A
Authority
AU
Australia
Prior art keywords
preosteoclasts
osteoclasts
precursor cells
differentiation
inducing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU30562/99A
Inventor
Tomoko Maeda
Takahiro Ochi
Ryuji Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Publication of AU3056299A publication Critical patent/AU3056299A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0643—Osteoclasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

Abstract

This invention relates to: a method for differentiating osteoclast precursor cells (preosteoclasts) into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cells; a method for isolating preosteoclasts, which comprises culturing peripheral blood or joint fluid in the absence of cytokine for 1 to 3 weeks; an preosteoclasts, which is obtainable by the above method; a method for differentiating the preosteoclasts obtained by the above method into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cell; an osteoclast, which is obtainable by the above method; a method for screening agents for treating metabolic bone diseases, which comprises using the preosteoclasts or the osteoclasts as described above; and agents for treating metabolic bone diseases, which is obtainable by the above screening method.
AU30562/99A 1998-04-08 1999-04-06 Methods for isolating osteoclast precursor cells and inducing the differentiation of the same into osteoclasts Abandoned AU3056299A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP9596298 1998-04-08
JP10/95962 1998-04-08
JP17040798 1998-06-18
JP10/170407 1998-06-18
PCT/JP1999/001803 WO1999053023A1 (en) 1998-04-08 1999-04-06 Methods for isolating osteoclast precursor cells and inducing the differentiation of the same into osteoclasts

Publications (1)

Publication Number Publication Date
AU3056299A true AU3056299A (en) 1999-11-01

Family

ID=26437128

Family Applications (1)

Application Number Title Priority Date Filing Date
AU30562/99A Abandoned AU3056299A (en) 1998-04-08 1999-04-06 Methods for isolating osteoclast precursor cells and inducing the differentiation of the same into osteoclasts

Country Status (7)

Country Link
US (2) US6861257B1 (en)
EP (1) EP1070757B1 (en)
JP (1) JP4271857B2 (en)
AT (1) ATE288477T1 (en)
AU (1) AU3056299A (en)
DE (1) DE69923550T2 (en)
WO (1) WO1999053023A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097121A1 (en) * 2001-05-28 2002-12-05 Nordic Bioscience A/S Method for assaying osteoclast recruitment and resorption
WO2006128029A2 (en) * 2005-05-25 2006-11-30 University Of Virginia Patent Foundation Production of osteoclasts from adipose tissues
US20070082395A1 (en) * 2005-10-11 2007-04-12 Biancamaria Baroli Production of normal human osteoclasts
AU2006304392B2 (en) 2005-10-18 2014-05-01 National Jewish Health Conditionally immortalized long-term stem cells and methods of making and using such cells
KR20170078862A (en) 2008-05-16 2017-07-07 타이가 바이오테크놀로지스, 인코포레이티드 Antibodies and processes for preparing the same
CN102083970B (en) 2008-07-21 2014-10-22 泰加生物工艺学公司 Differentiated anucleated cells and method for preparing the same
DE102008036060A1 (en) * 2008-08-04 2010-02-25 Technische Universität Dresden Cell-based method and means for bone formation
CA2735522C (en) 2008-08-28 2017-04-18 Taiga Biotechnologies, Inc. Modulators of myc, methods of using the same, and methods of identifying agents that modulate myc
SG11201500429QA (en) 2012-07-20 2015-03-30 Taiga Biotechnologies Inc Enhanced reconstitution and autoreconstitution of the hematopoietic compartment
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
WO2017011456A1 (en) * 2015-07-16 2017-01-19 Velo3D, Inc. Material-fall three-dimensional printing
IL293611A (en) 2016-12-02 2022-08-01 Taiga Biotechnologies Inc Nanoparticle formulations
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
US11478532B2 (en) 2017-12-03 2022-10-25 National Centre For Cell Science Therapeutic intervention for osteoporosis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06508987A (en) 1991-04-09 1994-10-13 インディアナ・ユニバーシティ・ファンデーション Systems and methods for supporting hematopoietic cells
CA2122141C (en) 1991-10-23 2007-07-17 Shelly Heimfeld Methods for selectively expanding stem cells
US6103522A (en) * 1994-07-20 2000-08-15 Fred Hutchinson Cancer Research Center Human marrow stromal cell lines which sustain hematopoiesis
US5830682A (en) * 1994-09-09 1998-11-03 Zymogenetics Preparation of immortalized cells
AU3586595A (en) 1994-09-09 1996-03-27 Zymogenetics Inc. Preparation of immortalized cells

Also Published As

Publication number Publication date
EP1070757A4 (en) 2003-04-09
US6861257B1 (en) 2005-03-01
EP1070757A1 (en) 2001-01-24
EP1070757B1 (en) 2005-02-02
DE69923550D1 (en) 2005-03-10
ATE288477T1 (en) 2005-02-15
DE69923550T2 (en) 2006-02-16
US20050003528A1 (en) 2005-01-06
WO1999053023A1 (en) 1999-10-21
JP4271857B2 (en) 2009-06-03

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase