AU2927792A - 2-pyridinol derivatives and their use as medicaments - Google Patents

2-pyridinol derivatives and their use as medicaments

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Publication number
AU2927792A
AU2927792A AU29277/92A AU2927792A AU2927792A AU 2927792 A AU2927792 A AU 2927792A AU 29277/92 A AU29277/92 A AU 29277/92A AU 2927792 A AU2927792 A AU 2927792A AU 2927792 A AU2927792 A AU 2927792A
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compound
formula
group
hereinbefore defined
reacting
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Kenneth John Murray
Roderick Alan Porter
Hunter Douglas Prain
Brian Herbert Warrington
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SmithKline Beecham Ltd
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SmithKline Beecham Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/62—Oxygen or sulfur atoms
    • C07D213/63—One oxygen atom
    • C07D213/64—One oxygen atom attached in position 2 or 6
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/08—Bronchodilators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/576—Six-membered rings
    • C07F9/58—Pyridine rings

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Cardiology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Hospice & Palliative Care (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pulmonology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Pyridine Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Description

2-PYRIDINOL DERIVATIVES AND THEIR USE AS MEDICAMENTS.
The present invention relates to pyridinol derivatives, processes for their preparation, intermediates in their preparation, their use as medicaments and to pharmaceutical compositions comprising them.
The compounds of this invention are agonists of a cyclic AMP- dependent protein kinase (cA-PrK) (see J. Biol. Chem., 1989, 264, 8443 - 8446) and are of use in combatting such
conditions where such agonism is thought to be beneficial. They are likely to have anti-proliferative, anti-aggregatory, cholesterol-lowering, smooth muscle relaxant, positive lusitropic, anti-allergic or anti-inflammatory activities. They are likely to be useful in the treatment of
cardiovascular diseases where there is a component of
diastolic failure, cancer, psoriasis, atheroschlerosis, thrombosis, re-stenosis, chronic reversible lung disease such as asthma and bronchitis, allergic disease such as allergic asthma, allergic rhinitis and urticaria or gut motility disorders such as irritable bowel syndrome.
Accordingly the present invention provides compounds of the formula (1) :
or pharmaceutically acceptable salts thereof, wherein R0 is OH or a bioprecursor thereof, R1 is A0CO2H, P (Z) (OH) (OR2), SO2H or SO3H or a bioprecursor thereof,
A0 is CH2, CHF, CF2, CR3 (OR4) , CO or C(OR5) (OR6),
R2 is phenyl, C3-5cycloalkyl, C3-5cycloalkylC1-4alkyl, or C1-8alkyl optionally substituted by C1-4alkoxy,
R3 is H, methyl or ethyl,
R4 is H or C1-3alkyl,
R5 and R6 are each C1-3alkyl or together form a 1,2- ethanediyl group or 1,3-propanediyl group,
Z is O or S, and
Ar is phenyl optionally substituted by one to three groups independently selected from C1-6alkyl, C2-6alkenyl,
C1-6alkoxy, C3-6alkenyloxy, C3-6cycloalkyl, C3-6cycloalkoxy, C1-6alkylthio, phenyl, phenylthio, benzyloxy,
C1-6polyfluoroalkyl, C1-6polyfluoroalkoxy, halo, N(R7)2 or NHCOR7 wherein R7 is H or C1-6alkyl, or -X(CH2)nY- attached to adjacent carbon atoms of the phenyl ring wherein X and Y are independently CH2 or 0 and n is 1 to 3, wherein said C1-6alkyl, C2-6alkenyl or C1-6alkoxy groups can be
independently substituted by OH, C1-6alkoxy, C3-6cycloalkyl, N(R7)2, CO2R7 or CON(R7)2. Bioprecursors of the groups R0 and R1 are derivatives thereof which are convertible in vivo into the groups R0 and R1.
A suitable bioprecursor of the group R^ is OR*5 wherein R^ is C1-4alkyl, arylC1-4alkyl (for example phenylC1-4alkyl such as benzyl), C1-4alkanoyl (for example acetyl), arylC1-4alkanoyl (for example phenyl C1-4alkanoyl such as benzoyl),
arylsulphonyl (for example optionally substituted
phenylsulphonyl or toluenesulphonyl) or C1-4alkylsulphonyl (for example methylsulphonyl). When R1 is A0CO2H a suitable bioprecursor is A0CO2R9 wherein R9 is an ester-forming group. When R1 is P (Z) (OH) (OR2) a suitable bioprecursor is
P (Z) (OR2)2 wherein Z and R2 are as hereinbefore defined or P(Z) (OR2) (OR10) wherein R10 is an O-protecting group.
Suitable O-protecting groups include pivaloyloxymethyl, propionyloxymethyl and pivalolyloxycarbonyloxymethyl.
Alternatively bioprecursors of the groups R0 and R1 are those formed when R1 and R0 are linked together to form a cyclic structure such that R1-R0 is A1CO2 or A2OCH2O, in which : A1 is CH2, CHF, CF2, CR3(OR4), CO or C(OR5) (OR6),
A2 is P(Z)OR2 or CR3 (CO2R9), and
R2 to R6, R9 and Z are as hereinbefore defined.
Suitably R0 is hydroxy or OR8, preferably hydroxy.
Suitably R1 is A0CO2H or A0CO2R9. Suitably R1 is P (Z) (OH) (OR2) or P(Z) (OR2)2.
Suitably R1 is SO2H or SO3H.
Suitably R1 and R0 are linked together such that R1-R0 is A1CO2.
Suitably R1 and R0 are linked together such that R1-R0 is A2OCH2O. By the term alkyl is meant both straight- and branched- chain alkyl. By the term C1-6polyfluoroalkyl is meant a C1-6alkyl group having at least one hydrogen replaced with fluoro, e.g. CF3 or CF2CF2H. Suitably R2 is methyl, ethyl, propyl, butyl, pentyl, hexyl, 2-methoxyethyl, phenyl, cyclopropyl, cyclobutyl, cyclopentyl or cyclopropylmethyl.
Suitably R3 is H, methyl or ethyl, preferably H or methyl.
Suitably R4 is H, methyl, ethyl or propyl, preferably H or methyl.
Suitably R5 and R6 are independently methyl, ethyl or propyl, preferably together they form a 1,2-ethanediyl group.
Preferably Z is O.
Suitably R9 is C1-4alkyl optionally substituted by hydroxy, e.g. 2-hydroxyethyl or arylC1-4alkyl (for example
phenylC1-4alkyl such as benzyl).
Suitably Ar is phenyl optionally mono-substituted by a group as hereinbefore defined, for example in the 2,3, or 4
positions by C1-6alkyl, C1-6alkoxy, C3-6alkenyloxy,
C1-6alkylthio, phenyl, phenylthio, benzyloxy, CF3, halo or NHCOR7.
Suitably Ar is phenyl di-substituted by any groups as
hereinbefore defined, for example in the 3,4-, 3, 5-, 2,3-, 2, 4- or 2,5-, positions by groups independently selected from
C2-6alkenyl, C1-6alkoxy, C3-6cycloalkoxy, halo, -X(CH2)nY- or C1-6alkoxyC1-6 alkoxy. Suitably Ar is phenyl trisubstituted by any groups as
hereinbefore defined, for example in the 2,3,4-, 2,3,5-, or 3,4,5-positions by groups independently selected from
C2-6alkenyl, C1-6alkoxy or halo. Examples of C1-6alkoxy include methoxy, ethoxy, propoxy, butoxy, or pentyloxy.
Examples of C1-6alkyl include methyl, ethyl, propyl, butyl, isobutyl or pentyl.
Examples of halo include fluoro, chloro, bromo or iodo.
Particular compounds of this invention include : ethyl [6-(3-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]- phosphonate, ethyl [6-(4-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]- phosphonate,
[6-(3,5-diethoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]acetic acid, ethyl [6-(2-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]- phosphonate, ethyl [6-(2,3-dipropoxyphenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate, n-propyl [6-(3-n-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate or
{6-[4-methoxy-3-(E-1-propenyl)phenyl]-2-oxo-1,2-dihydro-3-pyridyl}acetic acid, and pharmaceutically acceptable salts thereof.
This invention covers all tautomeric, geometric and optical isomeric forms of compounds of formula (1). In particular when R0 is hydroxy the compound can exist in its keto tautomeric form :
Compounds of the formula (1) can form pharmaceutically acceptable base addition salts with metal ions, such as alkali metals for example sodium or potassium, or with an ammonium ion.
In order to use a compound of the formula (1) or a
pharmaceutically acceptable salt thereof for the treatment of humans and other mammals it is normally formulated in
accordance with standard pharmaceutical practice as a
pharmaceutical composition. Compounds of formula (1) and their pharmaceutically
acceptable salts may be administered in standard manner for the treatment of the indicated diseases, for example orally, sublingually, parenterally, transdermally, rectally, via inhalation or via buccal administration.
Compounds of formula (1) and their pharmaceutically
acceptable salts which are active when given orally or via buccal administration can be formulated appropriately in dosage forms such as liquids, syrups, tablets, capsules and lozenges. An oral liquid formulation will generally consist of a suspension or solution of the compound or salt in a liquid carrier for example, ethanol, glycerine or water with a flavouring or colouring agent. Where the composition is in the form of a tablet, any pharmaceutical carrier routinely used for preparing solid formulations may be used. Examples of such carriers include starch, celluloses, lactose, sucrose and magnesium stearate. Where the composition is in the form of a capsule, any routine encapsulation is suitable, for example using the aforementioned carriers in a hard gelatin capsule shell. Where the composition is in the form of a soft gelatin shell capsule, any pharmaceutical carrier routinely used for preparing dispersions or suspensions may be
considered, for example aqueous gums, celluloses, silicates or oils and are incorporated in a soft gelatin capsule shell.
Typical parenteral compositions consist of a solution or suspension of the compound or salt in a sterile aqueous or non-aqueous carrier optionally containing a parenterally acceptable oil or solubilising agent, for example
polyethylene glycol, polyvinylpyrrolidone, lecithin,
2-pyrrolidone, cyclodextrin, arachis oil, or sesame oil.
A typical suppository formulation comprises a compound of formula (1) or a pharmaceutically acceptable salt thereof which is active when administered in this way, with a binding and/or lubricating agent, for example polymeric glycols, gelatins, cocoa-butter or other low melting vegetable waxes or fats or their synthetic analogues. Typical transdermal formulations comprise a conventional aqueous or non-aqueous vehicle, for example a cream,
ointment, lotion or paste or are in the form of a medicated plaster, patch or membrane. Typical compositions for inhalation are in the form of a solution, suspension or emulsion that may be administered in the form of an aerosol using a conventional propellant such as dichlorodifluoromethane or trichlorofluoromethane, or are in the form of a powder for insufflation.
Preferably the composition is in unit dosage form, for
example a tablet, capsule or metered aerosol dose, so that the patient may administer to himself a single dose. Each dosage unit for oral administration contains suitably from 0.001 mg/Kg to 30 mg/Kg, and preferably from 0.005 mg/Kg to 15 mg/Kg, and each dosage unit for parenteral
administration contains suitably from 0.001 mg/Kg to 10 mg/Kg, of a compound of formula (1) or a pharmaceutically acceptable salt thereof calculated as the free acid.
The daily dosage regimen for oral administration is suitably about 0.001 mg/Kg to 120 mg/Kg, of a compound of formula (1) or a pharmaceutically acceptable salt thereof calculated as the free acid. The daily dosage regimen for parenteral administration is suitably about 0.001 mg/Kg to 40 mg/Kg, for example about 0.005 mg/Kg to 10 mg/Kg, of a compound of the formula (1) or a pharmaceutically acceptable salt thereof calculated as the free acid. The active ingredient may be administered as required for example from 1 - 8 times a day or by infusion. The compositions of the invention are agonists of a cA-PrK and are of use in combatting such conditions where such agonism is thought to be beneficial. Such conditions can be treated by administration orally, sublingually topically, rectally, parenterally or by
inhalation. For administration by inhalation dosages are controlled by a valve, are administered as required and for an adult are conveniently in the range 0.1 - 5.0 mg of a compound of the formula (1) or a pharmaceutically acceptable salt thereof.
The compounds of this invention may be co-administered with other pharmaceutically active compounds, for example in combination, concurrently or sequentially. Conveniently the compounds of this invention and the other active compound or compounds are formulated in a single pharmaceutical
composition. Examples of compounds which may be included in pharmaceutical compositions with the compounds of the formula (1) are bronchodilators such as sympathomimetic amines for example isoprenaline, isoetharine, sulbutamol, phenylephrine and ephedrine or xanthine derivatives for example
theophylline and aminophylline, anti-allergic agents for example disodium cromoglycate, histamine H1-antagonists, drugs used in the treatment of cancer such as those which inhibit the synthesis of or inactivate DNA, for example methotrexate, fluoracil, cisplatin, actinomycin D, anti-atherschlerotic agents for example cholesterol lowering drugs such as HMGCoA reductase inhibitors, bile acid sequestrants, drugs for the treatment of psoriasis, for example retinoids, anthralin, anti-inflammatories for example cortiscosteroids, non-steroid anti-inflammatories such as aspirin,
antithrombotics for example dipyridamole, or fibrinolytic agents.
In another aspect the present invention provides a process for the preparation of compounds of the formula (1) or pharmaceutically acceptable salts thereof, which process comprises : a) for compounds wherein R1 is A0CO2H or A0CO2R9 and : i) A0 is CR3(OR4),
reacting in the presence of a strong base a compound of the formula (2) :
wherein R11 is methyl and Ar is as hereinbefore defined, with a compound of the formula (3) :
R3COCO2R9 (3) wherein R3 and R9 are as hereinbefore defined to form a compound of the formula (4) :
wherein R12 is CR3(OH)CO2R9 and R3, R9, R11, and Ar are as hereinbefore defined and thereafter optionally reacting with a C1-3alkylating agent to form the corresponding compound wherein R12 is CR3 (OC1-3alkyl)CO2R9, ii) A0 is CO,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with a compound of the formula (5) : R9O2CCO2R9 (5) wherein R9 is as hereinbefore defined to form a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11 and Ar are as hereinbefore defined, iii) A0 is CH(OH),
reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a reducing agent to form the corresponding compound wherein R12 is CH(OH)CO2R9, iv) A0 is CH2,
reacting a compound of the formula (4) wherein R12 is COCO2H or COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a suitable reducing agent to form the corresponding compound wherein R12 is CH2CO2H, v) A0 is C(OR5) (OR6),
reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a C1-3alcohol, 1,2-ethanediol or 1,3-propanediol to form the corresponding compound wherein R12 is C(OR5) (OR6)CO2R9, vi) A0 is CF2,
reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a fluorinating agent to form the corresponding compound wherein R11 is CF2CO2R9, or vii) A0 is CHF,
reacting a compound of the formula (4) wherein R12 is
CH(OH)CO2R9 and R9, R11, and Ar are as hereinbefore defined with a fluorinating agent to form the corresponding compound wherein R12 is CHFCO2R9, and thereafter optionally : º converting the group OR11 into OH ° converting the group A0CO2R9 into A0CO2H; or b) for compounds wherein R1 is CH2CO2H,
converting a compound of the formula (6) :
wherein R13 is acetyl and Ar is as hereinbefore defined into the corresponding compound wherein R13 is CH2CO2H; or c) for compounds wherein R1 is CH(OR4)CO2H reacting a compound of the formula (4) wherein R12 is -CH(OH)CN with a C1-3alkylating agent and/or converting the group CN into
CO2H, and optionally converting the group OR11 into OH; or d) for compounds wherein R1 is P(O) (OH) (OR2), hydrolysing a compound of the formula (6) wherein R13 is P (O) (OR2)2 and R2, and Ar are as hereinbefore defined; or e) for compounds wherein R1 is P (S) (OH) (OR2) , converting a compound of the formula (6) wherein R13 is P (O) (NHR14) (OR2) and R14 is phenyl or C1-4alkyl and Ar is as hereinbefore defined into the corresponding compound wherein R13 is
P (S) (OH) (OR2); or f) for compounds where R1 is SO3H,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with sulphuryl chloride or a chemical equivalent thereof and optionally converting the group OR11 into OH; or g) for compounds wherein R1 is SO2H,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with sulphur dioxide and optionally converting the group OR11 into OH; or h) for compounds wherein R1 is as defined for compounds of the formula (1) reacting in the presence of a palladium catalyst a compound of the formula (7):
wherein Rb is a group R1 as hereinbefore defined or a
precursor thereof and Ra is R0 or OR11 as hereinbefore
defined and L is a leaving group with a compound of the formula (8): ArB (OH) 2 (8) or a chemical equivalent thereof wherein Ar is as
hereinbefore defined and then, if necessary, converting a precursor of R1 into R1 and/or converting the group OR11 into OH, and optionally thereafter :
° forming a bioprecursor of R0 and/or R1
° forming a pharmaceutically acceptable salt. Suitably a compound of the formula (2) is reacted with a strong base such as lithium diisopropylamide, or a C1-4alkyl lithium or aryl lithium such as mesityl lithium in an organic solvent such as tetrahydrofuran, diethylether or
dimethoxyethane with cooling (-100° - 0°C) to form the anion thereof. The strong base may be formed in situ, for example by the addition of a C1-4alkyl lithium e.g. methyllithium followed by a catalytic quantity of diisopropylamine.
The anion of a compound of the formula (2) is suitably reacted with, a compound of the formula (3) or a compound of the formula (5) in an organic solvent such as
tetrahydrofuran, diethylether or dimethoxyethane with cooling (-100° to 0°C) to form a compound of the formula (4) wherein R12 is CR3(OH)CO2R9 or COCO2R9 respectively. A suitable compound of the formula (3) is ethylpyruvate, or ethyl
glyoxylate or a chemical equivalent thereof and a suitable compound of the formula (5) is diethyloxalate.
A compound of the formula (4) wherein R12 is CR3(OH)CO2R9 is suitably reacted with a C1-3alkylating agent such as
iodomethane, iodopropane or dimethylsulphate in the presence of a base such as sodium hydride or potassium hydroxide in an organic solvent such as dimethylformamide or
dimethylsulphoxide at elevated (e.g. 30 - 80°C) or preferably ambient temperature to form the corresponding compound wherein R12 is CR3 (OC1-3alkyl)CO2R9. When potassium hydroxide is used as base the CO2R9 group may be directly converted to carboxy.
A compound of the formula (4) wherein R12 is COCO2R9 is suitably reacted with a reducing agent such as sodium borohydride, or diisobutylaluminium hydride in an organic solvent such as dichloromethane, a C1-4alcohol e.g. ethanol, or acetic acid or mixtures thereof at ambient or elevated temperature (e.g. 30 - 80°C), or with cooling (e.g. 0 - 5°C) to form the corresponding compound wherein R12 is
CH(OH)CO2R9. A compound of the formula (4) wherein R12 is COCO2H or
COCO2R9 is suitably reacted with a reducing agent such as a zinc amalgam in hydrochloric acid in the absence of a solvent or in a solvent such as ethanol, acetic acid or dioxan and hydrogen chloride gas at ambient or elevated temperature (e.g. 40-100°C) to form the corresponding compound wherein R12 is CH2CO2H. Under these reaction conditions the CO2R9 group is converted to carboxy.
A compound of the formula (4) wherein R12 is COCO2R9 is suitably reacted with a C1-3alcohol, 1,2-ethanediol or 1,3- propanediol in the presence of an acid catalyst such as paratoluenesulphonic acid, concentrated sulphuric acid or anhydrous hydrogen chloride, at ambient or elevated
temperature to form the corresponding compound wherein R12 is C(OR5)(OR6)CO2R9.
A compound of the formula (4) wherein R12 is COCO2R9 or
CHOHCO2R9 is suitably reacted with a fluorinating agent such as diethylaminosulphur trifluoride in an organic solvent such as a halohydrocarbon or an ether, such as glyme, or THF at ambient or elevated temperature (e.g. 30-60°C) to form the corresponding compound where R12 is CF2CO2R9 or CHFCO2R9 respectively. A compound of the formula such as wherein OR11 is methoxy can suitably be converted to the corresponding compound wherein OR11 is hydroxy by reaction with sodium iodide and
chlorotrimethylsilane in an organic solvent such as
acetonitrile, or a halohydrocarbon e.g. dichloromethane or chloroform at elevated (e.g. 30 - 80°C) or preferably ambient temperature. This method is particularly suitable for preparing compounds of the formula (1) wherein R1 is A0CO2R9 since the ester-forming group R9 is not hydrolysed under the reaction conditions. Another method utilises sodium
thiomethoxide in an organic solvent such as dimethylformamide at an elevated temperature for example 40 - 120°C. The more forcing conditions of this method are suitable for preparing compounds of formula (1) wherein R1 is A0CO2H.
A compound of the formula (4) wherein R12 is A0CO2R9 can suitably be converted to the corresponding compound wherein R12 is A0CO2H by reaction with an aqueous base such as sodium or potassium hydroxide at ambient or elevated temperature (e.g. 40 - 120°). This method is particularly suitable for preparing compounds of the formula (1) wherein R0 is methoxy since the OR11 group is not hydrolysed. Another hydrolysis method utilises aqueous acid such as concentrated
hydrochloric acid at an elevated temperature (e.g. 40 - 120°C) which provides directly compounds of the formula (1) wherein R0 is hydroxy and R1 is A0CO2H.
Suitably a compound of the formula (6) wherein R13 is acetyl is converted to the corresponding compound where R13 is
CH2CO2H by reaction with sulphur and morpholine
at elevated temperature (e.g. 50 - 200°C), followed by
hydrolysis with an aqueous base such as sodium hydroxide at elevated temperature, preferably at the reflux temperature of the reaction mixture.
Suitably a compound of formula (4) where R12 is -CH(OH)CN is reacted with a C1-3alkylating agent as hereinbefore described followed by reaction with aqueous mineral acid such as 5N hydrochloric acid at ambient or elevated temperature preferably at refux in order to prepare the corresponding compound where R12 is CH(OC1-3alkyl) CO2H. The alkylation can be omitted if the corresponding compound where R12 is CH(OH)CO2H is desired. During the hydrolysis of the CN group the OR11 group may be converted to hydroxy. If not and if desired this group can be converted to hydroxy as
hereinbefore described.
A compound of the formula (4) wherein R12 is -CH(OH)CN can be prepared by reacting the corresponding compound wherein R12 is -CHO with a source of cyanide such as potassium cyanide in the presence of acid such as hydrochloric acid preferably at ambient temperature. A compound of the formula (4) or (6) where R12 or R13 is CHO is suitably prepared by reacting the corresponding compound wherein R12 or R13 is cyano with a suitable reducing agent such as diisobutylaluminium hydride followed by aqueous acidic work-up.
Suitably a compound of the formula (6) wherein R13 is
P (O) (OR2)2 is hydrolysed by reaction with an aqueous base such as sodium hydroxide optionally in a cosolvent such as a C1-4alcohol at an elevated temperature (e.g. 40-100°C), preferably at the reflux temperature of the reaction mixture.
Suitably a compound of the formula (6) wherein R13 is
P (O) (NHR14) (OR2) is converted to the corresponding compound wherein R9 is P(S) (OH) (OR2) by reaction with a strong base such as sodium hydride in an organic solvent such as
dimethoxyethane at ambient or elevated temperature, (e.g. 40 - 100°C) followed by reaction with carbon disulphide.
Suitably the anion of a compound of the formula (2) prepared as hereinbefore described is reacted with sulphuryl chloride or a chemical equivalent thereof or with sulphur dioxide in an organic solvent such as tetrahydrofuran with cooling (-100° - 0°C) to form after aqueous work-up a compound of the formula (4) wherein R12 is SO3H or SO2H respectively and OR11 is methoxy which if desired can be converted to the
corresponding compound wherein OR11 is hydroxy as
hereinbefore described. Suitably a compound of the formula (7) is reacted with a compound of the formula (8) in the presence of 1-50 mole %, preferably 2-10 mole %, of a palladium catalyst and a base such as triethylamine, sodium bicarbonate, or aqueous sodium carbonate and optionally lithium chloride in an organic solvent such as dimethylformamide, dimethoxyethane,
acetonitrile, toluene, tetrahydrofuran, ethanol, or mixtures thereof, at elevated temperature, (e.g. 30-150°C), preferably at the reflux temperature of the mixture. Suitably L1 is halo for example iodo, bromo or chloro or a trifluoromethanesulphonate. Subsequently the OR11 group can be converted to hydroxy as hereinbefore described for compounds of formula (4). Examples of palladium catalysts that can be used include: tetrakis (triphenylphosphine) palladium (Pd[PPh3]4),
bis(triphenylphosphine)palladium dichloride (Pd[PPh3]2Cl2),
[1,4-bis-(diphenylphosphine)butane]palladium dichloride
(Pd(dppb)Cl2),
[1,3-bis-(diphenylphosphine)propane]palladium dichloride (Pd(dppp)Cl2),
[1,2-bis-(diphenylphosphine)ethane]palladium dichloride
(Pd(dppe)Cl2),
bis (tri-o-tolylphosphine) palladium diacetate or dichloride
(Pd(totp) (OAc)2 or Pd(totp)Cl2), or
1,1 -bis(diphenylphosphine)ferrocinopalladium diacetate or dichloride (Pd[dppf](OAc)2 or Pd[dppf]Cl2).
By a chemical equivalent of a compound of the formula (8) is meant a reagent that can couple the Ar group onto the pyridyl ring of a compound of the formula (7). For example aryl stannanes can be used, such as ArSnMe3 which can suitably be prepared by reacting a suitable aryl halide (such as ArBr or Arl) with a base such as t-butyl lithium followed by reaction with a trimethyl tin halide (e.g. Me3SnCl). Alternatively the aryl halide can be reacted with Me3SnSnMe3 in the presence of a palladium catalyst as hereinbefore described to prepare a suitable aryl stannane. When Rb is a group R1 as hereinbefore defined reaction of a compound of the formula (7) with a compound of the formula (8) results directly in compounds of the formula (1).
An example of a precursor for R1 is when Rb is hydrogen. In this situation reaction of a compound of the formula (7) with a compound of the formula (8) or a chemical equivalent thereof results in a compound of the formula (2) or a
compound of the formula (6) wherein R13 is hydrogen. Such compounds can then be converted into a compound of the formula (1) as herein described.
Other precursors for R1 include CN, CHO or COMe. Reaction of a compound of the formula (7) wherein Rb represents such a precursor with a compound of the formula (8) or a chemical equivalent thereof results in a compound of the formula (4) or a compound of the formula (6) wherein R12 or R13 is CN, CHO or COMe. Such compounds can be converted to compounds of the formula (1) as herein described. If desired a compound of the formula (1) wherein R1 is A0CO2H can be converted to the corresponding compound wherein R1 is A0CO2R9 by reaction with a compound R9OH wherein R9 is as hereinbefore defined. A compound of the formula (1) wherein R0 is OH can be
converted to the corresponding compound where R0 is OR8 by reaction with R8L2 wherein R8 is as hereinbefore defined and L2 is a leaving group such as halo e.g. bromo, chloro, iodo. If desired a compound of the formula (1) wherein R1 is
P (Z) (OR2) (OH) can be converted to the corresponding compound wherein R1 is P (Z) (OR2) (OR10) by reaction with a suitable
O-protecting agent in standard manner. For example the
compound can be reacted with a pivalolyloxymethyl halide. A compound of the formula (1) wherein R1-R0 is A1CO2 is suitably prepared by heating a compound of the formula (1) wherein R1 is A1CO2H and R0 is OH with a dehydrating agent such as acetic anhydride, at an elevated temperature (e.g. 40 - 200°C), preferably at the reflux temperature of the
reaction mixture.
A compound of the formula (1) wherein R1-R0 is A2OCH2O is suitably prepared by reacting a compound of the formula (1) wherein R1 is A2OH and R0 is OH with a dihalomethane such as diiodo- or dibromomethane in the presence of silver carbonate in an organic solvent such as dimethylformamide at an
elevated temperature (e.g. 40 - 120°C).
A compound of the formula (2) is suitably prepared by
reacting a compound of the formula (6) wherein R13 is
hydrogen with an O-methylating agent such as
dimethylformamide dimethylacetal in dimethylformamide or trimethylphosphite at an elevated temperature (e.g. 40 -
120°C) or with iodomethane and silver carbonate in toluene or chloroform. This method can also be used for converting compounds of the formula (6) into corresponding compounds of the formula (4) and compounds of formula (7) where Ra is hydroxy to compounds of formula (7) where Ra is OR11.
A compound of the formula (6) wherein R13 is cyano, acetyl or hydrogen is suitably prepared by reaction of a compound of the formula (9) :
ArCOCH=CHL3 (9) with a compound of the formula (10) : R15CH2CONH2 (10) wherein R15 is cyano, acetyl or hydrogen respectively, and L3 is a displaceable group and Ar is as hereinbefore defined. Suitably L3 in a compound of the formula (9) is hydroxy or a derivative thereof for example L3 is protected hydroxy such as silyloxy, an acid residue (for example C1-6alkanoyloxy) or an ether residue (for example methoxy or ethoxy).
Alternatively L3 is a secondary amino group, for example di-C1-6alkylamino such as dimethylamino or a cyclic amino group such as piperidino, pyrrolidino or morpholino.
Preferably L3 is hydroxy or dimethylamino. Suitably an alkali metal (e.g. sodium) salt of a compound of the formula (9) wherein L3 is hydroxy is treated with a compound of the formula (10) under mildly alkaline aqueous conditions, for example in water in the presence of
piperidine and glacial acetic acid, at an elevated
temperature (e.g. 30 - 200°C), preferably at the reflux temperature of the reaction mixture.
Alternatively a compound of the formula (9) wherein L3 is a secondary amino group, for example dimethylamino, is treated with a compound of the formula (10) in a suitable solvent such as dimethylformamide, a C1-4alkanol or pyridine at an elevated temperature e.g. (30 - 200°C), preferably at the reflux temperature of the reaction mixture optionally in the presence of a base such as pyridine or an alkali metal
alkoxide, e.g. sodium methoxide.
Compounds of the formula (9) wherein L3 is hydroxy can
suitably be prepared by reaction under basic conditions of a compound of the formula (11) :
ArCOCH3 (11) wherein Ar is are as hereinbefore defined, with a compound of the formula HCOL4 wherein L4 is a leaving group.
Suitably L4 is ethoxy or methoxy. Conveniently a solution of a compound of the formula (11) and a compound of the formula HCOL3 in a suitable organic solvent such as diethyl ether is treated with a suitable base such as an alkali metal alkoxide, e.g. sodium methoxide at ambient temperature. The resulting reaction mixture is preferably extracted with water and the aqueous extract which contains the alkali metal salt of a compound of the formula (9) wherein L3 is hydroxy is then treated with a compound of the formula (10) as
hereinbefore described.
Compounds of the formula (9) wherein L3 is a secondary amino group (e.g. dimethylamino) can suitably be prepared by reacting a compound of the formula (11) with a compound of the formula HC(OR16)2L3 wherein R16 is C1-4alkyl and L3 is a secondary amino group (for example HC(OR16)2L3 is
N,N-dimethylformamide dimethyl or diethyl acetal).
Alternatively a compound of the formula (11) can be reacted with a compound of the formula HC(L3)3 (for example tris dimethylaminomethane).
A compound of the formula (6) wherein R13 is acetyl can also be prepared by reacting a compound of the formula (6) wherein R13 is cyano with methyl lithium followed by aqueous acidic work up with for example hydrochloric acid.
A compound of the formula (6) wherein R13 is hydrogen can also be prepared by heating a compound of formula (11) as hereinbefore defined with a C1-4alkyl propiolate (such as methyl propiolate) and ammonia in a solvent such as a
C1-4alkanol in a pressure vessel at elevated temperature (40-200°C) preferably at 100°C. Alternatively a compound of the formula (6) wherein R13 is hydrogen can be prepared by reacting a compound of the
formula (6) wherein R13 is cyano with orthophosphoric acid at an elevated temperature, e.g. 50 - 200°C. A compound of formula (4) wherein R12 is cyano is suitably prepared by reacting the anion of a compound of formula (2) wherein Ar and R11 are as hereinbefore defined with
dimethylformamide with cooling (e.g. -80 to 10°C), followed by ambient temperature and aqueous work-up. The resulting compound of formula (4) wherein R12 is carboxaldehyde is treated with hydroxylamine hydrochloride and sodium acetate in a suitable solvent such as ethanol or methanol at elevated temperature, e.g. 40-100°C, preferably at the reflux
temperature of the reaction mixture followed by dehydrating the product obtained for example by heating with acetic anhydride.
A compound of the formula (6) wherein R13 is cyano or acetyl and Ar is as hereinbefore defined can be suitably prepared by reaction of a compound of formula (4) wherein R12 is cyano or acetyl and R11 and Ar are as hereinbefore defined with a demethylating agent such as sodium
iodide/chlorotrimethylsilane in the absence of solvent or in an organic solvent such as acetonitrile or chloroform at an elevated temperature (e.g. 40 to 100°C) or at ambient
temperature.
A compound of the formula (6) wherein R13 is P (O) (OR2)2 can be prepared by treating a compound of the formula (2) wherein R11 is P (O) (OR2)2 with a strong base such as lithium
diisopropylamide in an organic solvent such as
tetrahydrofuran with cooling (e.g. -100-0°C). A compound of the formula (2) wherein R11 is P (O) (OR2)2 is suitably prepared by treating a compound of the formula (6) wherein R13 is hydrogen with a compound of the formula (12):
L5P(O)(OR2)2 (12) wherein L5 is a leaving group and R2 is as hereinbefore defined with a base such as diisopropylethylamine.
Suitably L5 is halo, for example chloro or bromo.
A compound of formula (2) wherein R11 is P (O) (OR2)2 can also be prepared by treating a compound of the formula (6) wherein R13 is hydrogen with a compound of the formula (13): HP (O) (OR2) 2 (13) wherein R2 is as hereinbefore defined in the presence of an amine base such as triethylamine, and carbon tetrachloride.
Alternatively, a compound of the formula (6) wherein R13 is P (O) (OR2)2 is suitably prepared by treating a compound of the formula (6) wherein R13 is hydrogen with a compound of the formula (12) in the presence of a strong base such as lithium diisopropylamide in an organic solvent such as
tetrahydrofuran with cooling (e.g. -100-0°C) without
isolation of the intermediate compound of the formula (2) wherein R11 is P (O) (OR2)2. A compound of formula (6) wherein R13 is hydrogen is suitably prepared by demethylating a compound of formula (2) as hereinbefore defined. Suitably a compound of formula (2) is treated with boron tribromide in an organic solvent such as dichloromethane or toluene with cooling (e.g. -80 to 10°C) followed by ambient temperature and aqueous work-up. Or a compound of formula (2) is treated with sodium iodide and chlorotrimethylsilane at ambient or elevated temperature (e.g. 40-80°C) conveniently ambient temperature in a solvent such as acetonitrile or dichloromethane.
A compound of the formula (6) wherein R13 is P (O) (NHR14) (OR2) can be prepared by reaction of a compound of the formula (6) wherein R13 is P (O) (OH) (OR2) with carbon tetrachloride, triphenylphosphine and aniline or a C1-4alkylamine in an organic solvent such as pyridine at ambient temperature or with cooling (e.g. -10 to 5°C). Alternatively a compound of the formula (6) where R13 is P (O) (OH) (OR2) can be reacted with dimethylformamide and oxalyl chloride in an organic solvent such as a halo hydrocarbon e.g. dichloromethane at ambient temperature, followed by reaction with aniline or a C1-4alkylamine preferably with cooling (-10 to 5°C).
Compounds of the formula (7) are known or can be prepared from a compound of the formula (14):
wherein Ra and L1 are as hereinbefore defined using similar methods to those described for preparing compounds of the formula (1). Thus, a compound of the formula (7) wherein Rb is P (O) (OR2)2 can be prepared by reacting a compound of the formula (14) wherein Ra is OH with a compound of the formula (12) or (13) in similar manner to the reaction of a compound of the formula (6) wherein R13 is hydrogen with a compound of the formula (12) or (13). If desired the group Ra can then be converted to OMe.
Similarly, a compound of the formula (14) where Ra is OMe can be treated in the presence of a strong base with a compound of the formula (3), a compound of the formula (5), sulphuryl chloride, sulphur dioxide or dimethylformamide to prepare a compound of the formula (7) wherein Rb is CR3 (OR4)CO2R9, COCO2R9, SO3H, SO2H or CHO respectively in similar manner to the corresponding reaction with a compound of the formula (2) as hereinbefore described. Particularly suitable as a strong base is lithium tetramethyl piperidide.
A compound of the formula (8) is suitably prepared by
reacting the organolithium or Grignard reagent, formed from a compound of the formula (15) :
Ar-L6 (15) wherein L6 is bromo or iodo and Ar is as hereinbefore defined with a tri-C1-4alkylborate such as trimethyl, tri-isopropyl or tri-n-butyl borate in an organic solvent such as diethyl ether or tetrahydrofuran with cooling (e.g. - 80-10°C).
The Ar group in compounds of the formula (2), (4), (6), (11) or (15) preferably (2), (4) or (11) may be appropriately functionalised by methods of aromatic substitution known in the art. For example, a bromo group may be introduced into a suitably substituted phenyl ring (eg. disubstituted in the 2- and 4-positions by electron-donating groups such as
C1-6alkoxy) by reaction with a brominating agent such as N-bromosuccinimide or bromine in a solvent such as
dimethylformamide. Alternatively a nitro group can be introduced into a phenyl ring by reaction with a suitable nitrating agent, such as nitronium tetrafluoroborate. Such a group can be readily hydrogenated to an amino group which if desired can be converted to a NHCOR7 group by reaction with LCOR7 wherein L is a leaving group and R7 is as hereinbefore defined. Suitable examples of the reagent LCOR include acid halides (L is halo eg. chloro or bromo) or acid anhydrides (L is OCOR7).
Other suitable functionalisations include the introduction of an allyl group ortho to a hydroxy substituent on a phenyl ring by reaction with an allyl halide, eg. bromide, to form an allyloxy derivative which on heating undergoes a Claisen rearrangement to form an ortho allyl hydroxy derivative. The hydroxy group can in turn be functionalised, eg. by reaction with a C1-6alkyl halide to form a C1-6alkoxy group. If desired, an allyl group can be converted to an E-1-propenyl group by reaction with a strong base, such as sodium
methoxide. This can occur during the conversion of a
compound of formula (11) to a compound of formula (6) as hereinbefore described if such a base is used. An
E-1-propenyl group can be cleaved to a formyl group by
reaction with an oxidising agent such as
N-methylmorpholine-N-oxide in the presence of a catalyst such as osmium tetroxide to form a 1,2, dihydroxypropyl group which on reaction with an oxidising agent such as sodium periodate forms the formyl group. Alternatively the E-1-propenyl group can be converted directly to a formyl group by reaction with a mixture of osmium tetroxide and sodium periodate or by reaction with ozone. A formyl group can in turn be further functionalised, for example it can be converted to a
hydroxymethyl group by reaction with a suitable reducing agent such as sodium borohydride, the hydroxymethyl group then being reacted further, eg. with a C1-6alkyl halide to form a C1-6alkoxymethyl group. Alternatively a formyl group can be reacted with a suitable Homer Wittig or Wittig reagent such as (R17O)2P(O)CH2CO2R17 or Ph3P=CHCO2R17 wherein R17 is C1-4alkyl to form a CH=CHCO2R17 group which can be optionally hydrolysed to a -CH=CHCO2H group. A -CH=CHCO2R17 group can be converted to a -CH=CHCON(R7)2 group by reaction with an amine HN(R7)2 or a chemical equivalent thereof wherein R7 is as hereinbefore defined. Alternatively a
-CH=CHCO2H group can be converted to an acid halide, eg. the acid chloride by reaction with oxalyl chloride, which can then be reacted with an amine HN(R7)2 or a chemical
equivalent thereof. An example of a chemical equivalent is ammonium hydroxide which will form a CH=CHCONH2 group.
Pharmaceutically acceptable base addition salts of the compounds of the formula (1) may be prepared by standard methods, for example by reacting a solution of the compound of the formula (1) with a solution of the base.
The following biological test methods, data and Examples serve to illustrate this invention. Cyclic-AMP Protein Kinase (cA-PrK) Agonist Activity
Type II cA-PrK was prepared from the cardiac muscle of a cow. The supernatant from a muscle homogenate (3 mis of 10 mM potassium phosphate, 1 mM EDTA per g tissue) was applied to a column of DEAE-cellulose equilibrated with the homogenisation buffer and the type II cA-PrK was eluted with homogenisation buffer containing 350 mM sodium chloride (Rannels et al., 1983, Methods Enzymol., 99, 55-62). Type II cA-PrK was assayed for phosphotransferase activity by incubating the enzyme at 30°C for 5 minutes with
[γ-32P]-adenosine triphosphate and a suitable peptide
substrate such as malantide (Malencik et al., 1983, Anal. Biochem., 132, 34-40). The reaction was terminated by the addition of hydrochloric acid and the [32P]-phosphopeptide quantified by spotting the reaction mixture onto
phosphocellulose papers. The concentration of compound required to give 10% phosphotransferase activation is given as the EC10 (μM). The compounds of Examples 1 to 7 had EC10 values in the range 1 to 30 μM.
Inhibition of Platelet Aggregation Human platelet-rich-plasma was separated from freshly drawn blood (in acid/citrate/dextrose) and treated with 100 μM acetylsalicylic acid for 15 minutes at 37°C. A washed platelet suspension was then prepared in a Hepes-isotonic saline buffer after a single centrifugation step and adjusted to a concentration of 1.5×108 cells/ml. Aliquots of this suspension were pre-incubated with compounds for 5 minutes at 37°C, then challenged with 1.0 μM U46619. The extent of aggregation after 2 minutes were expressed as a percentage of control and results obtained are expressed as an IC50
(concentration to cause 50% inhibition of platelet
aggregation, μM). The compounds of Examples 3 and 5 had IC50 values of 174 and 73 μM respectivley.
Inhibition of Spontaneous Contraction in Guinea-Pig Colon
Segments of isolated guinea-pig colon (2 cm) were suspended under 2 g tension in standard organ baths containing Krebs solution. The tissues were connected at the free end to isometric transducers which allow recording and display of developed tension on chart recorders. On-line computer capture and analysis was used to quantify the effects of test compounds on spontaneous contractions. Inhibitory responses were calculated as % maximum inhibition of spontaneous
contraction distance over 3 consecutive pre and post dose 2 minute readings. The concentration of compound which caused 50% inhibition of the spontaneous contraction is given as the EC50 (μM). Bronchodilatation - In vitro
Spiral strips of guinea-pig trachea were suspended in
standard organ baths containing Krebs solution. The tissues were connected at the free end to isometric transducers which allow recording and display of developed tension on chart recorders. Tension was allowed to develop spontaneously and concentrations of test compounds added in a cumulative fashion. The concentration of compound which caused 50% inhibition of the spontaneously developed tension is given as the IC50 (μM).
Measurement of cardiac muscle relaxation time in rabbit ventricle Papillary muscles from the right ventricle of female Albino New Zealand rabbits are mounted in standard organ baths containing oxygenated Krebs solution. One end of the muscle is connected to an isometric transducer which allowed
recording of contractile force and its first derivative on chart recorders. Test compounds are added to the bath in a cumulative manner. Relaxation time is calculated as the time taken from peak tension to the end of the contraction.
Compounds which cause a decrease in the relaxation time indicate a positive lusitropic effect of use in the treatment of cardiovascular diseases where there is a component of diastolic failure such as congestive heart failure, angina, hypertension and cardiomyopathy. Example 1
Ethyl [6-(3-bromophenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate
(a) 6-(3-bromoρhenyl)-3-cyanopyridin-2(1H)-one (9.6g) was heated in orthophosphoric acid (40ml, 85%) at 170°C for
42 hours, cooled to room temperature and poured into water (200ml). The precipitated material was separated by
filtration, washed with water and recrystallised from
n-butanol to give 6-(3-bromophenyl)pyridin-2(1H)-one (4.18g). 1H-NMR (d6-DMSO) 6.49 (d, 1H), 6.89 (d, 1H), 7.44 (t,1H),
7.56-7.66(m,2H), 7.84(d,1H) and 8.06 (s, 1H). (b) To a suspension of 6-(3-bromophenyl)pyridin-2(1H)-one (1.54g) in tetrahydrofuran (8ml), cooled to -78°C, lithium diisopropylamide (4ml, 1.5M in tetrahydrofuran) was added over 20 minutes. After the addition was complete the mixture was warmed to 0°C, stirred for 30 minutes, recooled to -78°C diethyl chlorophosphate (0.83g) added and the solution rewarmed to 0°C. After 30 minutes the reaction mixture was recooled to -78 C lithium diisopropylamide (4ml, 1.5M in tetrahydrofuran) added, the mixture stirred at -78 C for 30 minutes and at 0°C for 30 minutes. The reaction mixture was quenched with 2N hydrochloric acid (20ml), diluted with ethyl acetate (200ml) and the organic separated, washed with water (2×50ml), dried (MgSO4) and solvent removed at reduced pressure. Column chromatography (silica,
dichloromethane/ethanol 15:1 eluant) of the residue followed by recrystallisation from ethanol gave diethyl
[6-(3-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate (0.37g) m.p.l22-123°C.
(c) Diethyl [6-(3-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate (0.34g) in ethanol (10ml) containing aqueous sodium hydroxide (8ml, 40%) was boiled for 2 hours cooled to room temperature and adjusted to pH 1 with 2N hydrochloric acid. The precipitated material was separated by filtration washed with water and ethanol and recrystallised from ethanol to give the title compound (0.1g) m.p. 228-229°C.
Example 2
Ethyl [6-(4-bromophenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate
(a) A mixture of 4'-bromoacetophenone (19.9g) and
dimethylformamide dimethylacetal (14.6ml) in
dimethylformamide (80ml) was heated at 130°C for 16 hours. The mixture was cooled to room temperature, diluted with ethyl acetate (300ml), washed with water (6 × 200ml), dried (MgSO4) and solvent removed at reduced pressure. The residue was dissolved in dimethylformamide (80ml), sodium methoxide (10.8g) and cyanoacetamide (8.4g) added and the mixture heated at 120°C until gas evolution ceased (about 1 hour). After pouring into 10% aqueous acetic acid (300ml), the precipitated 6-(4-bromophenyl)-3-cyanopyridin-2(1H)-one
(19.8g) m.p. >320°C was collected by filtration, washed with water and ethanol and dried. 1H NMR (DMSO-d6) 6.82 (d,1H), 7.70-7.80 (m,4H), 8;20(d,1H) and 12.81 (br.s,1H).
(b) From 6-(4-bromophenyl)-3-cyanopyridin-2(1H)-one
(6.88g), 6-(4-bromophenyl)pyridin-2(1H)-one (6.25g) was prepared according to the method of Example 1(a). 1H NMR (d6- DMSO) 6.44 (d,1H), 6.80(d,1H), 7.57 (t,1H), 7.67 (d,2H) and
7.77 (d,2H). (c) From 6-(4-bromophenyl)-3-cyanopyridin-2(1H)-one
(2.00g), diethyl [6-(4-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate (0.42g) m.p. 136-138°C after
recrystallisation from ethanol, was prepared according to the method of Example 1(b).
(d) From diethyl [6-(4-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate (0.37g), the title compound (0.19g)
m.p.240-241°C after recrystallisation from ethanol, was
prepared according to the method of Example 1 (c). Example 3
[6-(3,5-Diethoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]acetic acid.
(a) To a cooled (card ice/acetone bath) suspension of 3- cyano-6-(3,5-diethoxyphenyl)pyridin-2(1H)-one (2.84g) in tetrahydrofuran (30ml), methyl lithium (18ml of 1.3M in diethyl ether) was added over 10 minutes. When the addition was complete the mixture was warmed to 0°C and stirred for 4 hours giving a deep red solution. The solution was recooled
(card ice/acetone) treated with 5N hydrochloric acid (50ml) and stirred at room temperature overnight. The mixture was boiled until all the organic solvent had evaporated cooled to room temperature and the aqueous phase separated by
decanting. The residual oily solid was triturated with boiling ethanol to give 3-acetyl-6-(3,5-diethoxyphenyl)pyridin-2(1H)-one (2.3g). 1H NMR d(DMSO-d6) 1.34(t,6H), 2.58(s,3H), 4.09(q,4H), 6.61(s,1H), 6.83(d,1H), 6.96(s,2H) and 8.07(d,1H).
(b) 3-Acetyl-6-(3,5-diethoxyphenyl)pyridin-2(1H)-one (1.5g), sulphur (0.18g) and morpholine (2ml) were heated together at 140°C for 4 hours. 2N Sodium hydroxide (10ml) was added and the mixture boiled for 3 hours. The solution was cooled to room temperature, filtered (celite pad) and the filtrate washed with ethyl acetate (3 × 30ml). The aqueous phase was adjusted to pH 7 (carbon dioxide), filtered (celite pad), the filtrate adjusted to pH 1 and the product collected by filtration to give the title compound (0.34g) m.p. 245-247°C after recrystallisation from ethanol. 1H NMR d(DMSO-d6)
1.33(t,6H), 3.38(s,2H), 4.07(q,4H), 6.52(m,1H), 6.58(d,1H), 6.86(d,2H), 7.42(1H) and 12.00 (br. s, 2H) . Example 4
Ethyl [6-(2-propoxyphenyl)-2-oxo-l,2-dihydro-3- pyridyl]phosphonate.
(a) To a cooled (card ice/acetone) solution of 6-chloro-2- pyridinol (6.48g) in tetrahydrofuran, lithium
diisopropylamide (34ml of a 1.5M solution in cyclohexane) was added over 20 minutes. When the addition was complete the temperature was raised to 0°C for 30 minutes, recooled to - 78°C and diethyl chlorophosphate (8.1ml) added. The
temperature was again raised to 0°C and the reaction mixture stirred for a further 30 minutes. After recooling to -78°C, additional lithium diisopropylamide (34ml of a 1.5M solution in cyclohexane) was added the mixture stirred at -78°C for 90 minutes and at 0°C for 30 minutes. After quenching with 2N hydrochloric acid (50ml) the aqueous phase was extracted with ethyl acetate (3 × 50ml). The combined organic extracts were washed with water (3 × 50ml), dried (MgSθ4) and solvent removed at reduced pressure. The residue was column
chromatographed (silica gel, dichloromethane® 7%
ethanol/dichloromethane) to give diethyl (6-chloro-2-oxo-1,2- dihydro-3-pyridyl)phosphonate (13.0g). 1H NMR (CDCI3)
1.34(t,6H), 4.07-4.30 (m,4H), 6.86(dd,1H) and 7.90 (dd, 1H).
(b) A mixture of diethyl (6-chloro-2-oxo-1,2-dihydro-3-pyridyl)phosphonate (2.13g), silver carbonate (4.4g) and iodomethane (5.7g) was boiled for 2 hours. The mixture was diluted with dichloromethane (100ml), filtered (celite pad) and solvent removed at reduced pressure. The residue was column chromatographed (silica gel, 3%
ethanol/dichloromethane eluant) to give diethyl (6-chloro-2-methoxy-3-pyridyl)phosphonate (1.2g). 1H NMR (DMSO-d6)
1.24 (t,6H), 3.94(s,3H), 3.99-4.11 (m, 4H) , 7.24(dd,1H) and
8.05(dd,1H).
(c) To an aqueous solution of sodium bicarbonate (0.54g in ml of water), [1,4-bis(diphenylphosphino)butane]palladium (II) chloride (0.3g), 2-propoxyphenylboronic acid (0.58g, prepared according to the method of W.J.Thompson and
J.Gaudino, J. Org. Chem., 1984, 49, 5237) in dimethoxyethane (6ml) and diethyl (6-chloro-2-methoxy-3-pyridyl) phosphonate (0.56g) in dimethoxyethane (8ml) were added. The mixture was boiled for 4 hours, cooled to room temperature and diluted with water (100ml) and dichloromethane (100ml). The organic phase was separated and the aqueous phase extracted with dichloromethane (2 × 50ml). The combined organic extracts were washed with water (2 × 100ml), dried and solvent removed at reduced pressure. The residue was column chromatographed (silica gel, 5% ethanol/dichloromethane eluant) to give diethyl [6-(2-propoxyphenyl)-2-methoxy-3-pyridyl]phosphonate (0.48g). 1H NMR (DMSO-d6) 0.98(t,3H), 1.26(t,6H), 1.66- 1.84 (m,2H), 3.99(s,3H), 3.99-4.11 (m, 6H), 7.04-7.17 (m, 2H), 7.43(dt,1H), 7.70(dd,1H), 7.94(dd,1H) and 8.05 (dd, 1H).
(d) A mixture of diethyl [6-(2-propoxyphenyl)-2-methoxy-3- pyridyl]phosphonate (0.45g), sodium iodide (0.2g) and
chlorotrimethylsilane (0.14g) in chloroform (2ml) was boiled for 45 minutes. After diluting with 2N hydrochloric acid (10ml) the mixture was extracted with ethyl acetate (2 × 20ml) The combined organic extracts were washed with water (20ml) and 10% sodium bicarbonate (2 × 20ml), dried (MgSO4) and solvent removed at reduced pressure. The residue was recrystallised from dichloromethane to give diethyl [6-(2-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate
(0.12g) m.p.111-112°C. 1H NMR (DMSO-d6) 0.94(t,3H),
1.24(t,6H), 1.63-1.78(m,2H), 3.94-4.11 (m, 6H), 6.43(dd,1H), 7.05(t,1H)7.14(d,1H), 7.41-7.48 (m, 2H) and 7.93 (dd, 1H).
(e) From diethyl [6-(2-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate (0.12g), the title compound (0.007g), m.p. 182-183°C after recrystallisation from ethanol was prepared according to the method of Example 1 (c). 1H NMR (DMSO-d6) 0.94(t,3H), 1.19(t,3H), 1.64-1.76 (m, 2H), 3.91-4.07 (m, 4H), 6.46(dd,1H), 7.03(t,1H), 7.16(d,1H), 7.40-7.48 (m, 2H and
7.92 (dd, 1H). Example 5
Ethyl [6-(2,3-dipropoxyphenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate.
(a) From diethyl (6-chloro-2-methoxy-3-pyridyl)phosphonate (1.1g) and 2,3-dipropoxyphenylboronic acid (1.5g), diethyl [6-(2,3-dipropoxyphenyl)-2-methoxy-3-pyridyl]phosphonate (1.3g) was prepared according to the method of Example 4(c). Treatment of diethyl [6-(2,3-dipropoxyphenyl)-2-methoxy-3- pyridyl]phosphonate (1.05g) with sodium iodide (0.36g) and chlorotrimethylsilane (0.26g) according to the method of Example 4(d) gave diethyl [6-(2,3-dipropoxyphenyl)-2-oxo-1,2- dihydro-3-pyridylIphosphonate (0.14g) m.p.l06-107°C after column chromatography (dichloromethane eluant). 1H NMR (DMSO- d6) 0.80(t,3H), 1.02(t,3H), 1.24(t,6H), 1.46-1.60 (m,2H), 1.70-1.83 (m,2H), 3.83(t,2H), 3.96-4.10 (m, 6H), 6.36 (br.s, 1H), 6.95(d,1H), 7.08-7.18 (m,2H) and 7.93 (dd, 1H). (b) From diethyl [6-(2,3-dipropoxyphenyl)-2-oxo-1,2-dihydro╌ 3-pyridyl]phosphonate (0.07g), the title compound (0.04g) m.p. 129-130°C after recrystallisation from ethyl acetate was prepared according to the method of Example 1 (c). 1H NMR (DMSO-d6) 0.80 (t,3H), 1.02 (t,3H), 1.18 (t,3H), 1.44- 1.59 (m,2H), 1.70-1.84 (m,2H), 3.82 (t,2H), 3.93 (t,2H),
3.99 (t,2H), 6.44(br.s,1H), 6.98 (d,1H), 7.07-7.68 (2H) and 7.93 (dd, 1H).
Example 6 n-Propyl [6-(3-n-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate
(a) From 3-n-propoxybromobenzene (8.6g), 3-n-propoxyphenylboronic acid (4.8g) m.p. 109-110°C after
recrystallisation from ethyl acetate/hexane, was prepared according to the method of W. J. Thompson J. Guadino J. Org. Chem. 1984, 49, 5237. (b) From 3-n-propoxyphenylboronic acid (1.72g) and 2- methoxy-6-chloropyridine (0.86g), 2-methoxy-6-(3-n- propoxyphenyl)pyridine (2.13g) was prepared according to the method of Example 4(c). 1H NMR (DMSO-d6) 1.00 (t,3H),
1.75(m,2H), 3.95(s,3H), 4.) (t,2H), 6.77 (d,1H), 6.97(dd,1H), 7.36(t,1H), 7.50(d,1H), 7.63(s,1H), 7.66(d,1H) and
7.75 (t,1H).
(c) From 2-methoxy-6-(3-n-propoxyphenyl)pyridine (2.1g), 6- (3-n-propoxyphenyl)pyridin-2(1H)-one (1.21g) m.p.100-101°C after recrystallisation from ethanol, was prepared according to the method of Example 4 (d).
(d) From 6-(3-n-propoxyphenyl)pyridin-2(1H)-one (1.15g), di-n-propyl [6-(3-propoxyphenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate (0.08g) m.p. 128-129°C after
recrystallisation from ethyl acetate, was prepared according to the method of Example 1 (b) using di-n-propylchlorophosphate instead of diethyl chlorophosphate.
(e) From di-n-propyl [6-(3-propoxyphenyl)-2-oxo-1,2- dihydro-3-pyridyl]phosphonate (0.06g), the title compound
(0.03g) m.p. 160°C after recrystallisation from ethyl acetate, was prepared according to the method of Example 1(c).
Example 7
{6-[4-methoxy-3-(E-1-propenyl)phenyl]-2-oxo-1,2-dihydro-3-pyridyl}acetic acid
(a) From 3-cyano-6-[4-methoxy-3-(E-1-propenyl)phenyl]-pyridin-2(1H)-one (3.99g) (Patent WO 92/06085), 3-acetyl-6- [4-methoxy-3-(E-1-propenyl)phenyl]pyridin-2(1H)-one (3.1g) after recrystallisation from dimethylformamide/water, was prepared according to the method of Example 3(a)
(b) From 3-acetyl-6-[4-methoxy-3-(E-1-propenyl)phenyl]-pyridin-2(1H)-one (1.42g), the title compound (0.06g) m.p. 240-242°C , was prepared according to the method of Example 3(b).
Example 8
Pharmaceutical compositions for oral administration are prepared by combining the following :
% w/w
Ethyl [6-(3-bromophenyl)- 2-oxo-1,2-dihydro3
pyridyl]phosphonate 0.5 3.0 7.14 2% w/w Soya lecithin in soya
bean oil 90.45 88.2 84.41
Hydrogenated vegetable
shortening and beeswax 9.05 8.8 8.45
The formulations are then filled into individual soft gelatin capsules.
Example 9
A pharmaceutical composition for parenteral administration is prepared by dissolving the title compound of Example 2
(0.02 g) in polyethylene glycol 300 (25 ml) with heating. This solution is then diluted with water for injections Ph. Eur. (to 100 ml). The solution is then sterilised by
filtration through a 0.22 micron membrane filter and sealed in sterile containers.

Claims (10)

Claims
1. A compound of the formula (1) :
or a pharmaceutically acceptable salt thereof, wherein :
R0 is OH or a bioprecursor thereof,
R1 is A0CO2H, P(Z) (OH) (OR2), SO2H or SO3H or a bioprecursor thereof,
A0 is CH2, CHF, CF2, CR3(OR4), CO or C(OR5) (OR6),
R2 is phenyl, C3-5Cycloalkyl, C3-5cycloalkylC1-4alkyl, or C1-8alkyl optionally substituted by C1-4alkoxy,
R3 is H, methyl or ethyl,
R4 is H or C1-3alkyl, R5 and R6 are each C1-3alkyl or together form a 1,2-ethanediyl group or 1,3-propanediyl group,
Z is O or S, and Ar is phenyl optionally substituted by one to three groups independently selected from C1-6alkyl, C2-6alkenyl,
C1-6alkoxy, C3-6alkenyloxy, C3-6cycloalkyl, C3-6cycloalkoxy, C1-6alkylthio, phenyl, phenylthio, benzyloxy,
C1-6polyfluoroalkyl, C1-6polyfluoroalkoxy, halo, N(R7)2 or NHCOR7 wherein R7 is H or C1-6alkyl, or -X(CH2)nY- attached to adjacent carbon atoms of the phenyl ring wherein X and Y are independently CH2 or 0 and n is 1 to 3, wherein said C1-6alkyl, C2-6alkenyl or C1-6alkoxy groups can be
independently substituted by OH, C1-6alkoxy, C3-6cycloalkyl, N(R7)2, CO2R7 or CON(R7)2.
2. A compound according to claim 1 wherein R1 is A0CO2H or A0CO2R9 in which R9 is an ester-forming group.
3. A compound according to claim 1 wherein R1 is P(Z) (OH) (OR2) or P(Z) (OR2)2.
4. A compound according to claims 1 wherein R1 is SO2H or SO3H.
5. A compound according to claim 1 wherein R1 and R0 are linked together such that R1-R0 is A1CO2
in which A1 is CH2, CHF, CF2, CR3(OR4), CO or C(OR5) (OR6).
6. A compound according to claim 1 wherein R1 and R0 are linked together such that R1-R0 is A2OCH2O in which A2 is
P(Z) (OR2) or CR3(CO2R9) and R9 is an ester-forming group.
7. A compound according to claim 1 which is : ethyl [6-(3-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]- phosphonate, ethyl [6-(4-bromophenyl)-2-oxo-1,2-dihydro-3-pyridyl]- phosphonate,
[6-(3,5-diethoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]acetic acid, ethyl [6-(2-propoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]-phosphonate, ethyl [6-(2,3-dipropoxyphenyl)-2-oxo-1,2-dihydro-3-pyridyl]phosphonate, n-propyl [6-(3-n-propoxyphenyl)-2-oxo-1,2-dihydro-3- pyridyl]phosphonate, or
{6-[4-methoxy-3-(E-1-propenyl)phenyl]-2-oxo-1,2-dihydro-3- pyridyl}acetic acid,
or a pharmaceutically acceptable salt thereof.
8. A compound according to any one of claims 1 to 7 for use as a medicament.
9. A pharmaceutical composition which comprises a compound according to any one of claims 1 to 7 and a
pharmaceutically acceptable carrier.
10. A process for preparing a compound of the formula (1) as defined in claim 1 or a pharmaceutically acceptable salt thereof which process comprises : a) for compounds wherein R1 is A0CO2H or A0CO2R9 and : i) A0 is CR3(OR4),
reacting in the presence of a strong base a compound of the formula (2) :
wherein R11 is methyl and Ar is as defined in claim 1, with a compound of the formula (3) :
R3COCO2R9 (3) wherein R3 is as defined in claim 1 and R9 is an esterforming group to form a compound of the formula (4) : wherein R12 is CR3(OH)CO2R9 and R3, R9, R11, and Ar are as hereinbefore defined and thereafter optionally reacting with a C1-3alkylating agent to form the corresponding compound wherein R12 is CR3(OC1-3alkyl)CO2R9, ii) A0 is CO,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with a compound of the formula (5) :
R9O2CCO2R9 (5) wherein R9 is as hereinbefore defined to form a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11 and Ar are as hereinbefore defined, iii) A0 is CH(OH),
reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a reducing agent to form the corresponding compound wherein R12 is CH(OH)CO2R9, iv) A0 is CH2,
reacting a compound of the formula (4) wherein R12 is COCO2H or COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a suitable reducing agent to form the corresponding compound wherein R12 is CH2CO2H, v) A0 is C(OR5)(OR6), reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a
Cl-3alcohol, 1,2-ethanediol or 1,3-propanediol to form the corresponding compound wherein R12 is C(OR5) (OR6)CO2R9, vi) A0 is CF2,
reacting a compound of the formula (4) wherein R12 is COCO2R9 and R9, R11, and Ar are as hereinbefore defined with a fluorinating agent to form the corresponding compound wherein R11 is CF2CO2R9, or vii) A0 is CHF,
reacting a compound of the formula (4) wherein R12 is
CH(OH)CO2R9 and R9, R11, and Ar are as hereinbefore defined with a fluorinating agent to form the corresponding compound wherein R12 is CHFCO2R9, and thereafter optionally : ° converting the group OR11 into OH
° converting the group A0CO2R9 into A0CO2H; or b) for compounds wherein R1 is CH2CO2H,
converting a compound of the formula (6) :
wherein R13 is acetyl and Ar is as hereinbefore defined into the corresponding compound wherein R13 is CH2CO2H; or c) for compounds wherein R1 is CH(OR4)CO2H reacting a compound of the formula (4) wherein R12 is -CH(OH)CN with a C1-3alkylating agent and/or converting the group CN into CO2H, and optionally converting the group OR11 into OH; or d) for compounds wherein R1 is P (O) (OH) (OR2), hydrolysing a compound of the formula (6) wherein R13 is P (O) (OR2)2, R2 is as defined in claim 1 and Ar is as hereinbefore defined; or e) for compounds wherein R1 is P(S) (OH) (OR2), converting a compound of the formula (6) wherein R13 is P (O) (NHR14) (OR2) and R14 is phenyl or C1-4alkyl and Ar is as hereinbefore defined into the corresponding compound wherein R13 is
P (S) (OH) (OR2); or f) for compounds where R1 is SO3H,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with sulphuryl chloride or a chemical equivalent thereof and optionally converting the group OR11 into OH; or g) for compounds wherein R1 is SO2H,
reacting in the presence of a strong base a compound of the formula (2) as hereinbefore defined with sulphur dioxide and optionally converting the group OR11 into OH; or h) for compounds wherein R1 is as defined for compounds of the formula (1) reacting in the presence of a palladium catalyst a compound of the formula (7):
wherein Rp is a group R1 as defined in claim 1 or a precursor thereof and Ra is R0 or OR11 as hereinbefore defined and L1 is a leaving group with a compound of the formula (8) : ArB (OH) 2 (8) or a chemical equivalent thereof wherein Ar is as
hereinbefore defined and then, if necessary, converting a precursor of R1 into R1 and/or converting the group OR11 into OH, and optionally thereafter :
° forming a bioprecursor of R0 and/or R1
° forming a pharmaceutically acceptable salt.
AU29277/92A 1991-11-20 1992-11-16 2-pyridinol derivatives and their use as medicaments Abandoned AU2927792A (en)

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