AU2023215671B2 - Method of modulating alkaloid content in tobacco plants - Google Patents

Method of modulating alkaloid content in tobacco plants

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Publication number
AU2023215671B2
AU2023215671B2 AU2023215671A AU2023215671A AU2023215671B2 AU 2023215671 B2 AU2023215671 B2 AU 2023215671B2 AU 2023215671 A AU2023215671 A AU 2023215671A AU 2023215671 A AU2023215671 A AU 2023215671A AU 2023215671 B2 AU2023215671 B2 AU 2023215671B2
Authority
AU
Australia
Prior art keywords
insdseq
insdqualifier
insdfeature
tobacco
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2023215671A
Other languages
English (en)
Other versions
AU2023215671A1 (en
Inventor
Francisco ANASTACIO DE ABREU E LIMA
Sara BEN KHALED
Javier GALDON-ARMERO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nicoventures Trading Ltd
Original Assignee
Nicoventures Trading Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nicoventures Trading Ltd filed Critical Nicoventures Trading Ltd
Publication of AU2023215671A1 publication Critical patent/AU2023215671A1/en
Application granted granted Critical
Publication of AU2023215671B2 publication Critical patent/AU2023215671B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A24B15/12—Chemical features of tobacco products or tobacco substitutes of reconstituted tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/08—Blending tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24F—SMOKERS' REQUISITES; MATCH BOXES; SIMULATED SMOKING DEVICES
    • A24F40/00—Electrically operated smoking devices; Component parts thereof; Manufacture thereof; Maintenance or testing thereof; Charging means specially adapted therefor
    • A24F40/20—Devices using solid inhalable precursors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2023215671A 2022-02-04 2023-01-30 Method of modulating alkaloid content in tobacco plants Active AU2023215671B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2201443.5 2022-02-04
GB202201443 2022-02-04
PCT/GB2023/050203 WO2023148475A1 (en) 2022-02-04 2023-01-30 Method of modulating alkaloid content in tobacco plants

Publications (2)

Publication Number Publication Date
AU2023215671A1 AU2023215671A1 (en) 2024-08-15
AU2023215671B2 true AU2023215671B2 (en) 2026-04-23

Family

ID=85199480

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2023215671A Active AU2023215671B2 (en) 2022-02-04 2023-01-30 Method of modulating alkaloid content in tobacco plants

Country Status (8)

Country Link
US (1) US20250137005A1 (https=)
EP (1) EP4472405A1 (https=)
JP (1) JP2025505606A (https=)
KR (1) KR20240132133A (https=)
AU (1) AU2023215671B2 (https=)
CA (1) CA3243712A1 (https=)
MX (1) MX2024009605A (https=)
WO (1) WO2023148475A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112094855A (zh) * 2020-09-27 2020-12-18 云南省烟草农业科学研究院 烟草细胞色素C基因NtCYP94B3s及对提高烟草茉莉酸含量的应用
CN112094864A (zh) * 2020-09-27 2020-12-18 云南省烟草农业科学研究院 利用烟草细胞色素C基因NtCYP94B3s提高烟草叶片数与生物量的方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2162738A (en) 1937-08-18 1939-06-20 Clarence E Mccoy Extracting nicotine from tobacco
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
KR100225087B1 (ko) 1990-03-23 1999-10-15 한스 발터라벤 피타아제의 식물내 발현
GB9524350D0 (en) 1995-11-29 1996-01-31 Lynxvale Ltd Enhancer-increased gene expression in plants
KR101413122B1 (ko) * 2005-02-28 2014-07-18 22엔디 센츄리 리미티드, 엘엘씨 식물의 니코틴 알칼로이드 수준의 감소
ES2582091T3 (es) 2005-10-18 2016-09-09 Precision Biosciences Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
EP2215223B1 (en) 2007-10-31 2013-05-01 Precision Biosciences, Inc. Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
US8953158B2 (en) 2009-09-04 2015-02-10 Danny S. Moshe Grading of agricultural products via hyper spectral imaging and analysis
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
PL2753202T3 (pl) 2011-09-06 2016-11-30 Podgrzewanie materiału przeznaczonego do palenia
CA2890160A1 (en) 2012-10-31 2014-05-08 Cellectis Coupling herbicide resistance with targeted insertion of transgenes in plants
EP3031921B1 (en) 2012-12-12 2025-03-12 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
GB2515502A (en) 2013-06-25 2014-12-31 British American Tobacco Co Apparatus and method
US20170298378A1 (en) * 2016-01-06 2017-10-19 National University Corporation Nagoya University Genes to provide environmental stress tolerance and the use thereof
MX2019015413A (es) 2017-06-23 2020-07-20 Univ Kentucky Res Found Metodo.
KR20250109246A (ko) * 2018-03-05 2025-07-16 알트리아 클라이언트 서비시즈 엘엘씨 바람직한 잎 품질과 함께 변경된 알칼로이드 수준을 갖는 담배 식물 및 제품을 생성하기 위한 조성물 및 방법
GB201818715D0 (en) * 2018-11-16 2019-01-02 British American Tobacco Investments Ltd Method
US12077765B2 (en) * 2019-01-24 2024-09-03 Altria Client Services Llc Tobacco plants comprising reduced nicotine and reduced tobacco specific nitrosamines
CN110951701B (zh) * 2019-09-18 2021-07-13 中国科学院昆明植物研究所 茉莉酸-异亮氨酸羟基化酶编码基因片段及其沉默载体在提高马铃薯产量中的应用
JP2022552261A (ja) * 2019-10-10 2022-12-15 アルトリア クライアント サーヴィシーズ リミテッド ライアビリティ カンパニー 変化したアルカロイドレベルを有するタバコ植物およびタバコ製品を作製するためのｑｐｔ操作に基づく組成物および方法
EP4069854A1 (en) * 2019-12-03 2022-10-12 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels
US20230159945A1 (en) * 2020-04-09 2023-05-25 R.J. Reynolds Tobacco Company Method
EP4152966A1 (en) * 2020-05-20 2023-03-29 Altria Client Services LLC A hybrid air and fire curing combination process to reduce harmful and potentially harmful constituents in cured tobacco

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112094855A (zh) * 2020-09-27 2020-12-18 云南省烟草农业科学研究院 烟草细胞色素C基因NtCYP94B3s及对提高烟草茉莉酸含量的应用
CN112094864A (zh) * 2020-09-27 2020-12-18 云南省烟草农业科学研究院 利用烟草细胞色素C基因NtCYP94B3s提高烟草叶片数与生物量的方法

Also Published As

Publication number Publication date
MX2024009605A (es) 2024-08-14
CA3243712A1 (en) 2023-08-10
KR20240132133A (ko) 2024-09-02
JP2025505606A (ja) 2025-02-28
EP4472405A1 (en) 2024-12-11
AU2023215671A1 (en) 2024-08-15
WO2023148475A1 (en) 2023-08-10
US20250137005A1 (en) 2025-05-01

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