AU2022375204A1 - Methods for determining respiratory infection risk - Google Patents
Methods for determining respiratory infection risk Download PDFInfo
- Publication number
- AU2022375204A1 AU2022375204A1 AU2022375204A AU2022375204A AU2022375204A1 AU 2022375204 A1 AU2022375204 A1 AU 2022375204A1 AU 2022375204 A AU2022375204 A AU 2022375204A AU 2022375204 A AU2022375204 A AU 2022375204A AU 2022375204 A1 AU2022375204 A1 AU 2022375204A1
- Authority
- AU
- Australia
- Prior art keywords
- individual
- biomarkers
- expression
- cbmcs
- tlr4 agonist
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 168
- 206010057190 Respiratory tract infections Diseases 0.000 title claims abstract description 98
- 230000014509 gene expression Effects 0.000 claims abstract description 168
- 239000000090 biomarker Substances 0.000 claims abstract description 138
- 101000669447 Homo sapiens Toll-like receptor 4 Proteins 0.000 claims abstract description 104
- 102100039360 Toll-like receptor 4 Human genes 0.000 claims abstract description 104
- 239000000556 agonist Substances 0.000 claims abstract description 95
- 210000004700 fetal blood Anatomy 0.000 claims abstract description 80
- 230000001965 increasing effect Effects 0.000 claims abstract description 77
- 210000005087 mononuclear cell Anatomy 0.000 claims abstract description 20
- 108010050904 Interferons Proteins 0.000 claims description 120
- 102000014150 Interferons Human genes 0.000 claims description 117
- 229940079322 interferon Drugs 0.000 claims description 117
- 239000002158 endotoxin Substances 0.000 claims description 100
- 229920006008 lipopolysaccharide Polymers 0.000 claims description 100
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 61
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 56
- 238000007637 random forest analysis Methods 0.000 claims description 55
- 101000598002 Homo sapiens Interferon regulatory factor 1 Proteins 0.000 claims description 42
- 102100036981 Interferon regulatory factor 1 Human genes 0.000 claims description 42
- 210000004027 cell Anatomy 0.000 claims description 32
- 238000002493 microarray Methods 0.000 claims description 30
- 102100023671 Coiled-coil domain-containing protein 194 Human genes 0.000 claims description 26
- 101000978238 Homo sapiens Coiled-coil domain-containing protein 194 Proteins 0.000 claims description 26
- 230000001105 regulatory effect Effects 0.000 claims description 26
- 238000003556 assay Methods 0.000 claims description 25
- 101000979565 Homo sapiens Protein NLRC5 Proteins 0.000 claims description 24
- 102100023432 Protein NLRC5 Human genes 0.000 claims description 24
- 102100023044 Cytosolic acyl coenzyme A thioester hydrolase Human genes 0.000 claims description 22
- 102100027346 GTP cyclohydrolase 1 Human genes 0.000 claims description 22
- 101000903587 Homo sapiens Cytosolic acyl coenzyme A thioester hydrolase Proteins 0.000 claims description 22
- 101000862581 Homo sapiens GTP cyclohydrolase 1 Proteins 0.000 claims description 22
- 101000599940 Homo sapiens Interferon gamma Proteins 0.000 claims description 22
- 101001027143 Homo sapiens Kelch domain-containing protein 7B Proteins 0.000 claims description 22
- 101000970017 Homo sapiens NEDD8 ultimate buster 1 Proteins 0.000 claims description 22
- 101000733743 Homo sapiens Phorbol-12-myristate-13-acetate-induced protein 1 Proteins 0.000 claims description 22
- 101001136981 Homo sapiens Proteasome subunit beta type-9 Proteins 0.000 claims description 22
- 101000735456 Homo sapiens Protein mono-ADP-ribosyltransferase PARP3 Proteins 0.000 claims description 22
- 101000644847 Homo sapiens Ubl carboxyl-terminal hydrolase 18 Proteins 0.000 claims description 22
- 101000911019 Homo sapiens Zinc finger protein castor homolog 1 Proteins 0.000 claims description 22
- 102100037850 Interferon gamma Human genes 0.000 claims description 22
- 102100037648 Kelch domain-containing protein 7B Human genes 0.000 claims description 22
- 102100021741 NEDD8 ultimate buster 1 Human genes 0.000 claims description 22
- 102100033716 Phorbol-12-myristate-13-acetate-induced protein 1 Human genes 0.000 claims description 22
- 102100035764 Proteasome subunit beta type-9 Human genes 0.000 claims description 22
- 102100034935 Protein mono-ADP-ribosyltransferase PARP3 Human genes 0.000 claims description 22
- 102100020726 Ubl carboxyl-terminal hydrolase 18 Human genes 0.000 claims description 22
- 102100026655 Zinc finger protein castor homolog 1 Human genes 0.000 claims description 22
- 238000011282 treatment Methods 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 21
- 102000039446 nucleic acids Human genes 0.000 claims description 20
- 108020004707 nucleic acids Proteins 0.000 claims description 20
- 150000007523 nucleic acids Chemical group 0.000 claims description 20
- -1 RNF213-AS1 Proteins 0.000 claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 101000613612 Homo sapiens Protein mono-ADP-ribosyltransferase PARP11 Proteins 0.000 claims description 16
- 102100040850 Protein mono-ADP-ribosyltransferase PARP11 Human genes 0.000 claims description 16
- 206010062106 Respiratory tract infection viral Diseases 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 15
- 241000709661 Enterovirus Species 0.000 claims description 14
- 102100034221 Growth-regulated alpha protein Human genes 0.000 claims description 13
- 101001069921 Homo sapiens Growth-regulated alpha protein Proteins 0.000 claims description 13
- 239000012634 fragment Substances 0.000 claims description 13
- 206010022000 influenza Diseases 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 241000725643 Respiratory syncytial virus Species 0.000 claims description 10
- 230000004913 activation Effects 0.000 claims description 10
- 102100025279 C-X-C motif chemokine 11 Human genes 0.000 claims description 9
- 101000858060 Homo sapiens C-X-C motif chemokine 11 Proteins 0.000 claims description 9
- 206010024971 Lower respiratory tract infections Diseases 0.000 claims description 9
- 238000004422 calculation algorithm Methods 0.000 claims description 7
- 210000001616 monocyte Anatomy 0.000 claims description 7
- 108010064218 Poly (ADP-Ribose) Polymerase-1 Proteins 0.000 claims description 6
- 102100023712 Poly [ADP-ribose] polymerase 1 Human genes 0.000 claims description 6
- 230000007423 decrease Effects 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 6
- 238000010801 machine learning Methods 0.000 claims description 6
- 208000035143 Bacterial infection Diseases 0.000 claims description 5
- 102100032420 Protein S100-A9 Human genes 0.000 claims description 5
- 208000022362 bacterial infectious disease Diseases 0.000 claims description 5
- GTVAUHXUMYENSK-RWSKJCERSA-N 2-[3-[(1r)-3-(3,4-dimethoxyphenyl)-1-[(2s)-1-[(2s)-2-(3,4,5-trimethoxyphenyl)pent-4-enoyl]piperidine-2-carbonyl]oxypropyl]phenoxy]acetic acid Chemical compound C1=C(OC)C(OC)=CC=C1CC[C@H](C=1C=C(OCC(O)=O)C=CC=1)OC(=O)[C@H]1N(C(=O)[C@@H](CC=C)C=2C=C(OC)C(OC)=C(OC)C=2)CCCC1 GTVAUHXUMYENSK-RWSKJCERSA-N 0.000 claims description 4
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 claims description 4
- 101001082065 Homo sapiens Interferon-induced protein with tetratricopeptide repeats 1 Proteins 0.000 claims description 4
- 102100027355 Interferon-induced protein with tetratricopeptide repeats 1 Human genes 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 210000004443 dendritic cell Anatomy 0.000 claims description 4
- 229940035032 monophosphoryl lipid a Drugs 0.000 claims description 4
- GXAFMKJFWWBYNW-OWHBQTKESA-N 2-[3-[(1r)-1-[(2s)-1-[(2s)-3-cyclopropyl-2-(3,4,5-trimethoxyphenyl)propanoyl]piperidine-2-carbonyl]oxy-3-(3,4-dimethoxyphenyl)propyl]phenoxy]acetic acid Chemical compound C1=C(OC)C(OC)=CC=C1CC[C@H](C=1C=C(OCC(O)=O)C=CC=1)OC(=O)[C@H]1N(C(=O)[C@@H](CC2CC2)C=2C=C(OC)C(OC)=C(OC)C=2)CCCC1 GXAFMKJFWWBYNW-OWHBQTKESA-N 0.000 claims description 3
- 102100036962 5'-3' exoribonuclease 1 Human genes 0.000 claims description 3
- 102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 claims description 3
- 108010028006 B-Cell Activating Factor Proteins 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 3
- 102100023006 Basic leucine zipper transcriptional factor ATF-like 2 Human genes 0.000 claims description 3
- 102100025634 Caspase recruitment domain-containing protein 16 Human genes 0.000 claims description 3
- 102100025308 GTPase RhebL1 Human genes 0.000 claims description 3
- 101000804879 Homo sapiens 5'-3' exoribonuclease 1 Proteins 0.000 claims description 3
- 101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 claims description 3
- 101000903615 Homo sapiens Basic leucine zipper transcriptional factor ATF-like 2 Proteins 0.000 claims description 3
- 101000933103 Homo sapiens Caspase recruitment domain-containing protein 16 Proteins 0.000 claims description 3
- 101001078130 Homo sapiens GTPase RhebL1 Proteins 0.000 claims description 3
- 101000840275 Homo sapiens Interferon alpha-inducible protein 27, mitochondrial Proteins 0.000 claims description 3
- 101001082058 Homo sapiens Interferon-induced protein with tetratricopeptide repeats 2 Proteins 0.000 claims description 3
- 101001034846 Homo sapiens Interferon-induced transmembrane protein 3 Proteins 0.000 claims description 3
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 claims description 3
- 101000941866 Homo sapiens Leucine-rich repeat neuronal protein 2 Proteins 0.000 claims description 3
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 claims description 3
- 101000641015 Homo sapiens Sterile alpha motif domain-containing protein 9 Proteins 0.000 claims description 3
- 101000639143 Homo sapiens Vesicle-associated membrane protein 5 Proteins 0.000 claims description 3
- 102100029604 Interferon alpha-inducible protein 27, mitochondrial Human genes 0.000 claims description 3
- 102100027303 Interferon-induced protein with tetratricopeptide repeats 2 Human genes 0.000 claims description 3
- 102100040035 Interferon-induced transmembrane protein 3 Human genes 0.000 claims description 3
- 102100027268 Interferon-stimulated gene 20 kDa protein Human genes 0.000 claims description 3
- 102100032653 Leucine-rich repeat neuronal protein 2 Human genes 0.000 claims description 3
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 claims description 3
- 102100034291 Sterile alpha motif domain-containing protein 9 Human genes 0.000 claims description 3
- 102100031484 Vesicle-associated membrane protein 5 Human genes 0.000 claims description 3
- 229960000470 omalizumab Drugs 0.000 claims description 3
- 229960000402 palivizumab Drugs 0.000 claims description 3
- 229960005205 prednisolone Drugs 0.000 claims description 3
- OIGNJSKKLXVSLS-VWUMJDOOSA-N prednisolone Chemical compound O=C1C=C[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OIGNJSKKLXVSLS-VWUMJDOOSA-N 0.000 claims description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 claims description 2
- 241000711573 Coronaviridae Species 0.000 claims description 2
- 102000008946 Fibrinogen Human genes 0.000 claims description 2
- 108010049003 Fibrinogen Proteins 0.000 claims description 2
- 108090000288 Glycoproteins Proteins 0.000 claims description 2
- 102000003886 Glycoproteins Human genes 0.000 claims description 2
- 241000606768 Haemophilus influenzae Species 0.000 claims description 2
- 102000002812 Heat-Shock Proteins Human genes 0.000 claims description 2
- 108010004889 Heat-Shock Proteins Proteins 0.000 claims description 2
- 229920002971 Heparan sulfate Polymers 0.000 claims description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 2
- 101001082070 Homo sapiens Interferon alpha-inducible protein 6 Proteins 0.000 claims description 2
- 102100027354 Interferon alpha-inducible protein 6 Human genes 0.000 claims description 2
- 241000588621 Moraxella Species 0.000 claims description 2
- 241001529936 Murinae Species 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 208000002606 Paramyxoviridae Infections Diseases 0.000 claims description 2
- 102100032442 Protein S100-A8 Human genes 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 claims description 2
- 229940012952 fibrinogen Drugs 0.000 claims description 2
- 229940047650 haemophilus influenzae Drugs 0.000 claims description 2
- 229920002674 hyaluronan Polymers 0.000 claims description 2
- 229960003160 hyaluronic acid Drugs 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000701161 unidentified adenovirus Species 0.000 claims description 2
- 210000003071 memory t lymphocyte Anatomy 0.000 claims 2
- 102000016605 B-Cell Activating Factor Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 description 143
- 239000000523 sample Substances 0.000 description 93
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 60
- 230000004044 response Effects 0.000 description 58
- 238000004458 analytical method Methods 0.000 description 54
- 229960002751 imiquimod Drugs 0.000 description 46
- DOUYETYNHWVLEO-UHFFFAOYSA-N imiquimod Chemical compound C1=CC=CC2=C3N(CC(C)C)C=NC3=C(N)N=C21 DOUYETYNHWVLEO-UHFFFAOYSA-N 0.000 description 46
- 102000040430 polynucleotide Human genes 0.000 description 43
- 108091033319 polynucleotide Proteins 0.000 description 43
- 239000002157 polynucleotide Substances 0.000 description 42
- 108020004414 DNA Proteins 0.000 description 38
- 230000003612 virological effect Effects 0.000 description 34
- 208000006673 asthma Diseases 0.000 description 33
- 230000006870 function Effects 0.000 description 33
- 230000000770 proinflammatory effect Effects 0.000 description 26
- 238000009396 hybridization Methods 0.000 description 25
- 208000024891 symptom Diseases 0.000 description 23
- 102000004127 Cytokines Human genes 0.000 description 22
- 108090000695 Cytokines Proteins 0.000 description 22
- 230000000694 effects Effects 0.000 description 21
- 208000015181 infectious disease Diseases 0.000 description 21
- 238000003752 polymerase chain reaction Methods 0.000 description 21
- 239000002773 nucleotide Substances 0.000 description 19
- 125000003729 nucleotide group Chemical group 0.000 description 19
- 238000012549 training Methods 0.000 description 19
- 238000010200 validation analysis Methods 0.000 description 19
- 108091034117 Oligonucleotide Proteins 0.000 description 16
- 206010047924 Wheezing Diseases 0.000 description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- 108020004999 messenger RNA Proteins 0.000 description 16
- 239000002299 complementary DNA Substances 0.000 description 15
- 230000010468 interferon response Effects 0.000 description 15
- 241000700605 Viruses Species 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 230000009385 viral infection Effects 0.000 description 14
- 101710163270 Nuclease Proteins 0.000 description 13
- 238000011161 development Methods 0.000 description 13
- 201000010099 disease Diseases 0.000 description 13
- 230000015788 innate immune response Effects 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 208000036142 Viral infection Diseases 0.000 description 12
- 238000003491 array Methods 0.000 description 12
- 229940088598 enzyme Drugs 0.000 description 12
- 238000000513 principal component analysis Methods 0.000 description 12
- 238000003559 RNA-seq method Methods 0.000 description 11
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 11
- 102100029904 Signal transducer and activator of transcription 1-alpha/beta Human genes 0.000 description 10
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000000875 corresponding effect Effects 0.000 description 10
- 239000013615 primer Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 230000009467 reduction Effects 0.000 description 10
- 230000000638 stimulation Effects 0.000 description 10
- 108010044012 STAT1 Transcription Factor Proteins 0.000 description 9
- 238000013459 approach Methods 0.000 description 9
- 230000000295 complement effect Effects 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 230000005713 exacerbation Effects 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- 238000012163 sequencing technique Methods 0.000 description 9
- 108010002352 Interleukin-1 Proteins 0.000 description 8
- 108091023040 Transcription factor Proteins 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 230000001404 mediated effect Effects 0.000 description 8
- 230000000241 respiratory effect Effects 0.000 description 8
- 210000002345 respiratory system Anatomy 0.000 description 8
- 238000002560 therapeutic procedure Methods 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- SYJGKVOENHZYMQ-UHFFFAOYSA-N Penoxsulam Chemical compound N1=C2C(OC)=CN=C(OC)N2N=C1NS(=O)(=O)C1=C(OCC(F)F)C=CC=C1C(F)(F)F SYJGKVOENHZYMQ-UHFFFAOYSA-N 0.000 description 7
- 102000040945 Transcription factor Human genes 0.000 description 7
- 241000271897 Viperidae Species 0.000 description 7
- 230000001154 acute effect Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000004186 co-expression Effects 0.000 description 7
- 238000003066 decision tree Methods 0.000 description 7
- 239000000975 dye Substances 0.000 description 7
- 230000010354 integration Effects 0.000 description 7
- 238000010606 normalization Methods 0.000 description 7
- 108020003175 receptors Proteins 0.000 description 7
- 238000003196 serial analysis of gene expression Methods 0.000 description 7
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 6
- 101000831496 Homo sapiens Toll-like receptor 3 Proteins 0.000 description 6
- 206010020751 Hypersensitivity Diseases 0.000 description 6
- 238000000585 Mann–Whitney U test Methods 0.000 description 6
- 102100024324 Toll-like receptor 3 Human genes 0.000 description 6
- 208000026935 allergic disease Diseases 0.000 description 6
- 230000007815 allergy Effects 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 238000010369 molecular cloning Methods 0.000 description 6
- 229920001184 polypeptide Polymers 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 102000005962 receptors Human genes 0.000 description 6
- 238000003757 reverse transcription PCR Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 102100027353 Interferon-induced helicase C domain-containing protein 1 Human genes 0.000 description 5
- 230000000840 anti-viral effect Effects 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 230000002596 correlated effect Effects 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 210000000987 immune system Anatomy 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 238000003012 network analysis Methods 0.000 description 5
- 230000036961 partial effect Effects 0.000 description 5
- 230000037361 pathway Effects 0.000 description 5
- 238000007781 pre-processing Methods 0.000 description 5
- 238000010839 reverse transcription Methods 0.000 description 5
- 206010006448 Bronchiolitis Diseases 0.000 description 4
- 102000019034 Chemokines Human genes 0.000 description 4
- 108010012236 Chemokines Proteins 0.000 description 4
- 108020004635 Complementary DNA Proteins 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 101710154606 Hemagglutinin Proteins 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 101001032342 Homo sapiens Interferon regulatory factor 7 Proteins 0.000 description 4
- 101000669402 Homo sapiens Toll-like receptor 7 Proteins 0.000 description 4
- 108010044240 IFIH1 Interferon-Induced Helicase Proteins 0.000 description 4
- 102100038070 Interferon regulatory factor 7 Human genes 0.000 description 4
- 101150064984 Irf1 gene Proteins 0.000 description 4
- 102000005348 Neuraminidase Human genes 0.000 description 4
- 108010006232 Neuraminidase Proteins 0.000 description 4
- 238000000636 Northern blotting Methods 0.000 description 4
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 4
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 4
- 101710176177 Protein A56 Proteins 0.000 description 4
- 206010037660 Pyrexia Diseases 0.000 description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 description 4
- 108010006785 Taq Polymerase Proteins 0.000 description 4
- 102100039390 Toll-like receptor 7 Human genes 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 208000029771 childhood onset asthma Diseases 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000006196 drop Substances 0.000 description 4
- 239000000185 hemagglutinin Substances 0.000 description 4
- 210000000867 larynx Anatomy 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 229940115272 polyinosinic:polycytidylic acid Drugs 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 230000019491 signal transduction Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 230000003827 upregulation Effects 0.000 description 4
- 102100025248 C-X-C motif chemokine 10 Human genes 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 3
- 239000003298 DNA probe Substances 0.000 description 3
- 102100033189 Diablo IAP-binding mitochondrial protein Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101000871228 Homo sapiens Diablo IAP-binding mitochondrial protein Proteins 0.000 description 3
- 102000043138 IRF family Human genes 0.000 description 3
- 108091054729 IRF family Proteins 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 201000007100 Pharyngitis Diseases 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 206010046306 Upper respiratory tract infection Diseases 0.000 description 3
- 208000002365 Viral Bronchiolitis Diseases 0.000 description 3
- 238000004873 anchoring Methods 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000000621 bronchi Anatomy 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 208000035475 disorder Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000003862 glucocorticoid Substances 0.000 description 3
- 238000013394 immunophenotyping Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 238000010208 microarray analysis Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 210000003800 pharynx Anatomy 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 208000023504 respiratory system disease Diseases 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 210000003437 trachea Anatomy 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- 108010081589 Becaplermin Proteins 0.000 description 2
- 102100021936 C-C motif chemokine 27 Human genes 0.000 description 2
- 101710112538 C-C motif chemokine 27 Proteins 0.000 description 2
- 101710098275 C-X-C motif chemokine 10 Proteins 0.000 description 2
- 102100036170 C-X-C motif chemokine 9 Human genes 0.000 description 2
- 206010008469 Chest discomfort Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 208000034657 Convalescence Diseases 0.000 description 2
- 208000000059 Dyspnea Diseases 0.000 description 2
- 206010013975 Dyspnoeas Diseases 0.000 description 2
- 108700039887 Essential Genes Proteins 0.000 description 2
- 238000000729 Fisher's exact test Methods 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 101001032341 Homo sapiens Interferon regulatory factor 9 Proteins 0.000 description 2
- 241000712431 Influenza A virus Species 0.000 description 2
- 102100038251 Interferon regulatory factor 9 Human genes 0.000 description 2
- 102000003816 Interleukin-13 Human genes 0.000 description 2
- 108090000176 Interleukin-13 Proteins 0.000 description 2
- 102000049772 Interleukin-16 Human genes 0.000 description 2
- 101800003050 Interleukin-16 Proteins 0.000 description 2
- 102000003810 Interleukin-18 Human genes 0.000 description 2
- 108090000171 Interleukin-18 Proteins 0.000 description 2
- 108010002386 Interleukin-3 Proteins 0.000 description 2
- 108010002586 Interleukin-7 Proteins 0.000 description 2
- 108090001007 Interleukin-8 Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 101710151805 Mitochondrial intermediate peptidase 1 Proteins 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 206010068319 Oropharyngeal pain Diseases 0.000 description 2
- 241000712464 Orthomyxoviridae Species 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 238000012341 Quantitative reverse-transcriptase PCR Methods 0.000 description 2
- 108020004518 RNA Probes Proteins 0.000 description 2
- 239000003391 RNA probe Substances 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 208000037656 Respiratory Sounds Diseases 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
- 108010081691 STAT2 Transcription Factor Proteins 0.000 description 2
- 108010017324 STAT3 Transcription Factor Proteins 0.000 description 2
- 102100023978 Signal transducer and activator of transcription 2 Human genes 0.000 description 2
- 102100024040 Signal transducer and activator of transcription 3 Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102100021669 Stromal cell-derived factor 1 Human genes 0.000 description 2
- 101710088580 Stromal cell-derived factor 1 Proteins 0.000 description 2
- 102100036922 Tumor necrosis factor ligand superfamily member 13B Human genes 0.000 description 2
- 206010047468 Viral lower respiratory tract infections Diseases 0.000 description 2
- 238000001793 Wilcoxon signed-rank test Methods 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 230000005875 antibody response Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000003123 bronchiole Anatomy 0.000 description 2
- 229940124630 bronchodilator Drugs 0.000 description 2
- 108091092328 cellular RNA Proteins 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000002790 cross-validation Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000007418 data mining Methods 0.000 description 2
- 230000005860 defense response to virus Effects 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 230000009025 developmental regulation Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 206010016256 fatigue Diseases 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 230000008088 immune pathway Effects 0.000 description 2
- 229940124622 immune-modulator drug Drugs 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000013016 learning Effects 0.000 description 2
- 238000007477 logistic regression Methods 0.000 description 2
- 230000004199 lung function Effects 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 201000009240 nasopharyngitis Diseases 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 210000001331 nose Anatomy 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000008506 pathogenesis Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 230000004962 physiological condition Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000007112 pro inflammatory response Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000000171 quenching effect Effects 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 2
- 238000012174 single-cell RNA sequencing Methods 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229940037128 systemic glucocorticoids Drugs 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- 241000712461 unidentified influenza virus Species 0.000 description 2
- 210000001260 vocal cord Anatomy 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 208000037874 Asthma exacerbation Diseases 0.000 description 1
- 102100022970 Basic leucine zipper transcriptional factor ATF-like Human genes 0.000 description 1
- 102100023013 Basic leucine zipper transcriptional factor ATF-like 3 Human genes 0.000 description 1
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 1
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 1
- 102100032367 C-C motif chemokine 5 Human genes 0.000 description 1
- 102100032366 C-C motif chemokine 7 Human genes 0.000 description 1
- 101710155834 C-C motif chemokine 7 Proteins 0.000 description 1
- 102100039398 C-X-C motif chemokine 2 Human genes 0.000 description 1
- 101710085500 C-X-C motif chemokine 9 Proteins 0.000 description 1
- 102100034798 CCAAT/enhancer-binding protein beta Human genes 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108010055166 Chemokine CCL5 Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 102100020986 DNA-binding protein RFX5 Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 206010013082 Discomfort Diseases 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102100023688 Eotaxin Human genes 0.000 description 1
- 101710139422 Eotaxin Proteins 0.000 description 1
- 241000160765 Erebia ligea Species 0.000 description 1
- 206010051998 Febrile infection Diseases 0.000 description 1
- 102000003817 Fos-related antigen 1 Human genes 0.000 description 1
- 108090000123 Fos-related antigen 1 Proteins 0.000 description 1
- 102100040510 Galectin-3-binding protein Human genes 0.000 description 1
- 102100037390 Genetic suppressor element 1 Human genes 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 1
- 102100028541 Guanylate-binding protein 2 Human genes 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 102100035833 Histo-blood group ABO system transferase Human genes 0.000 description 1
- 102100028404 Homeobox protein Hox-B4 Human genes 0.000 description 1
- 101000903742 Homo sapiens Basic leucine zipper transcriptional factor ATF-like Proteins 0.000 description 1
- 101000903609 Homo sapiens Basic leucine zipper transcriptional factor ATF-like 3 Proteins 0.000 description 1
- 101000858088 Homo sapiens C-X-C motif chemokine 10 Proteins 0.000 description 1
- 101000889128 Homo sapiens C-X-C motif chemokine 2 Proteins 0.000 description 1
- 101000947172 Homo sapiens C-X-C motif chemokine 9 Proteins 0.000 description 1
- 101000945963 Homo sapiens CCAAT/enhancer-binding protein beta Proteins 0.000 description 1
- 101001075432 Homo sapiens DNA-binding protein RFX5 Proteins 0.000 description 1
- 101000967904 Homo sapiens Galectin-3-binding protein Proteins 0.000 description 1
- 101001026271 Homo sapiens Genetic suppressor element 1 Proteins 0.000 description 1
- 101001058858 Homo sapiens Guanylate-binding protein 2 Proteins 0.000 description 1
- 101000802660 Homo sapiens Histo-blood group ABO system transferase Proteins 0.000 description 1
- 101000839788 Homo sapiens Homeobox protein Hox-B4 Proteins 0.000 description 1
- 101100286558 Homo sapiens IFIH1 gene Proteins 0.000 description 1
- 101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 1
- 101001082073 Homo sapiens Interferon-induced helicase C domain-containing protein 1 Proteins 0.000 description 1
- 101000990912 Homo sapiens Matrilysin Proteins 0.000 description 1
- 101000844245 Homo sapiens Non-receptor tyrosine-protein kinase TYK2 Proteins 0.000 description 1
- 101000979347 Homo sapiens Nuclear factor 1 X-type Proteins 0.000 description 1
- 101000979338 Homo sapiens Nuclear factor NF-kappa-B p100 subunit Proteins 0.000 description 1
- 101001121539 Homo sapiens P2Y purinoceptor 14 Proteins 0.000 description 1
- 101000815628 Homo sapiens Regulatory-associated protein of mTOR Proteins 0.000 description 1
- 101100369992 Homo sapiens TNFSF10 gene Proteins 0.000 description 1
- 101000652747 Homo sapiens Target of rapamycin complex 2 subunit MAPKAP1 Proteins 0.000 description 1
- 101000708741 Homo sapiens Transcription factor RelB Proteins 0.000 description 1
- 101000648491 Homo sapiens Transportin-1 Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 101000997835 Homo sapiens Tyrosine-protein kinase JAK1 Proteins 0.000 description 1
- 241000534431 Hygrocybe pratensis Species 0.000 description 1
- 101150061045 IFIH1 gene Proteins 0.000 description 1
- 101150103227 IFN gene Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 1
- 108090000174 Interleukin-10 Proteins 0.000 description 1
- 102000003812 Interleukin-15 Human genes 0.000 description 1
- 108090000172 Interleukin-15 Proteins 0.000 description 1
- 108050003558 Interleukin-17 Proteins 0.000 description 1
- 102000013691 Interleukin-17 Human genes 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 108010002335 Interleukin-9 Proteins 0.000 description 1
- 241000721662 Juniperus Species 0.000 description 1
- 201000008197 Laryngitis Diseases 0.000 description 1
- 102100032352 Leukemia inhibitory factor Human genes 0.000 description 1
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 1
- 238000003657 Likelihood-ratio test Methods 0.000 description 1
- 239000007987 MES buffer Substances 0.000 description 1
- 102000043129 MHC class I family Human genes 0.000 description 1
- 108091054437 MHC class I family Proteins 0.000 description 1
- 102100030417 Matrilysin Human genes 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 108010057466 NF-kappa B Proteins 0.000 description 1
- 102000003945 NF-kappa B Human genes 0.000 description 1
- 206010028735 Nasal congestion Diseases 0.000 description 1
- 102100032028 Non-receptor tyrosine-protein kinase TYK2 Human genes 0.000 description 1
- 102100023049 Nuclear factor 1 X-type Human genes 0.000 description 1
- 102100023059 Nuclear factor NF-kappa-B p100 subunit Human genes 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 206010061876 Obstruction Diseases 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 102100025808 P2Y purinoceptor 14 Human genes 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 101150110096 RF1 gene Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 238000010240 RT-PCR analysis Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 206010061494 Rhinovirus infection Diseases 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 101150094092 STAT1 gene Proteins 0.000 description 1
- 108010077895 Sarcosine Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000003646 Spearman's rank correlation coefficient Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 230000006044 T cell activation Effects 0.000 description 1
- 102000046283 TNF-Related Apoptosis-Inducing Ligand Human genes 0.000 description 1
- 108700012411 TNFSF10 Proteins 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- GYDJEQRTZSCIOI-UHFFFAOYSA-N Tranexamic acid Chemical compound NCC1CCC(C(O)=O)CC1 GYDJEQRTZSCIOI-UHFFFAOYSA-N 0.000 description 1
- 230000010632 Transcription Factor Activity Effects 0.000 description 1
- 102100032727 Transcription factor RelB Human genes 0.000 description 1
- 102000000887 Transcription factor STAT Human genes 0.000 description 1
- 108050007918 Transcription factor STAT Proteins 0.000 description 1
- 102100028748 Transportin-1 Human genes 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 108010020713 Tth polymerase Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 102100033438 Tyrosine-protein kinase JAK1 Human genes 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000013567 aeroallergen Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 208000037883 airway inflammation Diseases 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000014102 antigen processing and presentation of exogenous peptide antigen via MHC class I Effects 0.000 description 1
- 239000008365 aqueous carrier Substances 0.000 description 1
- 239000010692 aromatic oil Substances 0.000 description 1
- 238000013528 artificial neural network Methods 0.000 description 1
- 229940127225 asthma medication Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 208000037893 chronic inflammatory disorder Diseases 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 210000004544 dc2 Anatomy 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000009274 differential gene expression Effects 0.000 description 1
- 125000005442 diisocyanate group Chemical group 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011985 exploratory data analysis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000011223 gene expression profiling Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000007938 immune gene expression Effects 0.000 description 1
- 230000008076 immune mechanism Effects 0.000 description 1
- 229940124644 immune regulator Drugs 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000007233 immunological mechanism Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007641 inkjet printing Methods 0.000 description 1
- 210000005007 innate immune system Anatomy 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- DLBFLQKQABVKGT-UHFFFAOYSA-L lucifer yellow dye Chemical compound [Li+].[Li+].[O-]S(=O)(=O)C1=CC(C(N(C(=O)NN)C2=O)=O)=C3C2=CC(S([O-])(=O)=O)=CC3=C1N DLBFLQKQABVKGT-UHFFFAOYSA-L 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 238000007837 multiplex assay Methods 0.000 description 1
- 238000000491 multivariate analysis Methods 0.000 description 1
- 208000013465 muscle pain Diseases 0.000 description 1
- 210000003928 nasal cavity Anatomy 0.000 description 1
- 239000000133 nasal decongestant Substances 0.000 description 1
- 208000010753 nasal discharge Diseases 0.000 description 1
- 239000007923 nasal drop Substances 0.000 description 1
- 229940100662 nasal drops Drugs 0.000 description 1
- 229940097496 nasal spray Drugs 0.000 description 1
- 239000007922 nasal spray Substances 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 210000003695 paranasal sinus Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 230000003864 performance function Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- SXADIBFZNXBEGI-UHFFFAOYSA-N phosphoramidous acid Chemical compound NP(O)O SXADIBFZNXBEGI-UHFFFAOYSA-N 0.000 description 1
- 229940068196 placebo Drugs 0.000 description 1
- 239000000902 placebo Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- RUOJZAUFBMNUDX-UHFFFAOYSA-N propylene carbonate Chemical compound CC1COC(=O)O1 RUOJZAUFBMNUDX-UHFFFAOYSA-N 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 208000020029 respiratory tract infectious disease Diseases 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 229940043230 sarcosine Drugs 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000018316 severe headache Diseases 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 201000009890 sinusitis Diseases 0.000 description 1
- 238000010181 skin prick test Methods 0.000 description 1
- 206010041232 sneezing Diseases 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000010972 statistical evaluation Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- CCEKAJIANROZEO-UHFFFAOYSA-N sulfluramid Chemical group CCNS(=O)(=O)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F CCEKAJIANROZEO-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012706 support-vector machine Methods 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 206010044008 tonsillitis Diseases 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/12—Pulmonary diseases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/24—Immunology or allergic disorders
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/26—Infectious diseases, e.g. generalised sepsis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/38—Pediatrics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/50—Determining the risk of developing a disease
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/60—Complex ways of combining multiple protein biomarkers for diagnosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Ecology (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Pulmonology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021903424A AU2021903424A0 (en) | 2021-10-26 | Methods for determining respiratory infection risk | |
| AU2021903424 | 2021-10-26 | ||
| PCT/AU2022/051283 WO2023070153A1 (en) | 2021-10-26 | 2022-10-26 | Methods for determining respiratory infection risk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2022375204A1 true AU2022375204A1 (en) | 2024-04-11 |
Family
ID=86160222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2022375204A Pending AU2022375204A1 (en) | 2021-10-26 | 2022-10-26 | Methods for determining respiratory infection risk |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240418708A1 (https=) |
| EP (1) | EP4423299A1 (https=) |
| JP (1) | JP2024539370A (https=) |
| KR (1) | KR20240093511A (https=) |
| CN (1) | CN118159668A (https=) |
| AU (1) | AU2022375204A1 (https=) |
| CA (1) | CA3233202A1 (https=) |
| WO (1) | WO2023070153A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN121075427A (zh) * | 2025-08-14 | 2025-12-05 | 中山大学·深圳 | 用于急性上呼吸道感染易感性的预测标志物及预测方法 |
-
2022
- 2022-10-26 CN CN202280071715.9A patent/CN118159668A/zh active Pending
- 2022-10-26 AU AU2022375204A patent/AU2022375204A1/en active Pending
- 2022-10-26 WO PCT/AU2022/051283 patent/WO2023070153A1/en not_active Ceased
- 2022-10-26 CA CA3233202A patent/CA3233202A1/en active Pending
- 2022-10-26 US US18/704,190 patent/US20240418708A1/en active Pending
- 2022-10-26 JP JP2024525930A patent/JP2024539370A/ja active Pending
- 2022-10-26 KR KR1020247013946A patent/KR20240093511A/ko active Pending
- 2022-10-26 EP EP22884788.5A patent/EP4423299A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA3233202A1 (en) | 2023-05-04 |
| CN118159668A (zh) | 2024-06-07 |
| JP2024539370A (ja) | 2024-10-28 |
| WO2023070153A1 (en) | 2023-05-04 |
| EP4423299A1 (en) | 2024-09-04 |
| KR20240093511A (ko) | 2024-06-24 |
| US20240418708A1 (en) | 2024-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12473597B2 (en) | Methods and systems for processing a nucleic acid sample | |
| US20240060132A1 (en) | Diagnosis of sepsis | |
| US11286529B2 (en) | Diagnostic methods for infectious disease using endogenous gene expression | |
| EP3362579B1 (en) | Methods for diagnosis of tuberculosis | |
| JP6995622B2 (ja) | 敗血症の診断法 | |
| US20150315643A1 (en) | Blood transcriptional signatures of active pulmonary tuberculosis and sarcoidosis | |
| JP2019520042A (ja) | 細菌感染及びウイルス感染を診断するための方法 | |
| US7910299B2 (en) | Methods for diagnosing systemic lupus erythematosus | |
| US20110183856A1 (en) | Diagnosis and Prognosis of Infectious Disease Clinical Phenotypes and other Physiologic States Using Host Gene Expression Biomarkers In Blood | |
| US12454717B2 (en) | Immune profiling using small volume blood samples | |
| US20170327874A1 (en) | Methods of Diagnosing Bacterial Infections | |
| CN115605608A (zh) | 帕金森氏病的检测方法 | |
| Rodríguez et al. | MyD88-dependent changes in the pulmonary transcriptome after infection with Chlamydia pneumoniae | |
| Jayavelu et al. | Single cell transcriptomic profiling of eosinophils and airway immune cells in childhood asthma | |
| US20240418708A1 (en) | Methods for determining respiratory infection risk | |
| US20160312285A1 (en) | Methods of identifying and treating subjects having acute respiratory distress syndrome | |
| US20230212699A1 (en) | Methods to detect and treat sars-cov-2 (covid19) infection | |
| Chauhan | RNA Seq analysis of macrophage in vitro infected with Mycobacterium bovis in cattle | |
| Morgun et al. | UltraRapid Communication |