AU2022276537A1 - NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING - Google Patents

NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING Download PDF

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Publication number
AU2022276537A1
AU2022276537A1 AU2022276537A AU2022276537A AU2022276537A1 AU 2022276537 A1 AU2022276537 A1 AU 2022276537A1 AU 2022276537 A AU2022276537 A AU 2022276537A AU 2022276537 A AU2022276537 A AU 2022276537A AU 2022276537 A1 AU2022276537 A1 AU 2022276537A1
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AU
Australia
Prior art keywords
oligonucleotide
sequence
nucleic acid
probe
hydrogel
Prior art date
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Pending
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AU2022276537A
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English (en)
Inventor
Karl A Deisseroth
Ethan B RICHMAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
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Leland Stanford Junior University
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Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of AU2022276537A1 publication Critical patent/AU2022276537A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/10Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Particle Accelerators (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2022276537A 2021-05-21 2022-05-20 NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING Pending AU2022276537A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163191455P 2021-05-21 2021-05-21
US63/191,455 2021-05-21
PCT/US2022/030370 WO2022246275A1 (en) 2021-05-21 2022-05-20 Next-generation volumetric in situ sequencing

Publications (1)

Publication Number Publication Date
AU2022276537A1 true AU2022276537A1 (en) 2023-12-07

Family

ID=84141872

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022276537A Pending AU2022276537A1 (en) 2021-05-21 2022-05-20 NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING

Country Status (6)

Country Link
US (1) US20240263228A1 (https=)
EP (1) EP4352254A4 (https=)
JP (1) JP2024521142A (https=)
AU (1) AU2022276537A1 (https=)
CA (1) CA3220740A1 (https=)
WO (1) WO2022246275A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
WO2023158821A2 (en) * 2022-02-17 2023-08-24 Vizgen, Inc. Methods of anchoring fragmented nucleic acid targets in a polymer matrix for imaging
CN117343993A (zh) * 2023-12-01 2024-01-05 中国科学院遗传与发育生物学研究所 原位检测样品中的靶核酸序列的方法
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012005595A2 (en) * 2010-07-09 2012-01-12 Wouter Leonard De Laat V3-d genomic region of interest sequencing strategies
US10982271B2 (en) * 2011-01-31 2021-04-20 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
WO2014030066A2 (en) * 2012-08-22 2014-02-27 Bernitz Mats Nilsson Methods for identifying nucleic acid sequences
EP4339956A3 (en) * 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
WO2018136856A1 (en) * 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
WO2019222284A1 (en) * 2018-05-14 2019-11-21 The Broad Institute, Inc. In situ cell screening methods and systems

Also Published As

Publication number Publication date
JP2024521142A (ja) 2024-05-28
EP4352254A1 (en) 2024-04-17
EP4352254A4 (en) 2025-04-16
CA3220740A1 (en) 2022-11-24
WO2022246275A1 (en) 2022-11-24
US20240263228A1 (en) 2024-08-08

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