AU2022276537A1 - NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING - Google Patents
NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING Download PDFInfo
- Publication number
- AU2022276537A1 AU2022276537A1 AU2022276537A AU2022276537A AU2022276537A1 AU 2022276537 A1 AU2022276537 A1 AU 2022276537A1 AU 2022276537 A AU2022276537 A AU 2022276537A AU 2022276537 A AU2022276537 A AU 2022276537A AU 2022276537 A1 AU2022276537 A1 AU 2022276537A1
- Authority
- AU
- Australia
- Prior art keywords
- oligonucleotide
- sequence
- nucleic acid
- probe
- hydrogel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Photoreceptors In Electrophotography (AREA)
- Particle Accelerators (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163191455P | 2021-05-21 | 2021-05-21 | |
| US63/191,455 | 2021-05-21 | ||
| PCT/US2022/030370 WO2022246275A1 (en) | 2021-05-21 | 2022-05-20 | Next-generation volumetric in situ sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2022276537A1 true AU2022276537A1 (en) | 2023-12-07 |
Family
ID=84141872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2022276537A Pending AU2022276537A1 (en) | 2021-05-21 | 2022-05-20 | NEXT-GENERATION VOLUMETRIC <i>IN SITU</i> SEQUENCING |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20240263228A1 (https=) |
| EP (1) | EP4352254A4 (https=) |
| JP (1) | JP2024521142A (https=) |
| AU (1) | AU2022276537A1 (https=) |
| CA (1) | CA3220740A1 (https=) |
| WO (1) | WO2022246275A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7502787B2 (ja) | 2018-04-09 | 2024-06-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インサイチュ遺伝子配列決定の方法 |
| WO2023158821A2 (en) * | 2022-02-17 | 2023-08-24 | Vizgen, Inc. | Methods of anchoring fragmented nucleic acid targets in a polymer matrix for imaging |
| CN117343993A (zh) * | 2023-12-01 | 2024-01-05 | 中国科学院遗传与发育生物学研究所 | 原位检测样品中的靶核酸序列的方法 |
| WO2025189030A1 (en) | 2024-03-07 | 2025-09-12 | Stellaromics, Inc. | Methods and systems for volumetric imaging |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012005595A2 (en) * | 2010-07-09 | 2012-01-12 | Wouter Leonard De Laat | V3-d genomic region of interest sequencing strategies |
| US10982271B2 (en) * | 2011-01-31 | 2021-04-20 | Roche Sequencing Solutions, Inc. | Methods of identifying multiple epitopes in cells |
| WO2014030066A2 (en) * | 2012-08-22 | 2014-02-27 | Bernitz Mats Nilsson | Methods for identifying nucleic acid sequences |
| EP4339956A3 (en) * | 2016-11-08 | 2024-06-19 | President and Fellows of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| WO2018136856A1 (en) * | 2017-01-23 | 2018-07-26 | Massachusetts Institute Of Technology | Multiplexed signal amplified fish via splinted ligation amplification and sequencing |
| WO2019222284A1 (en) * | 2018-05-14 | 2019-11-21 | The Broad Institute, Inc. | In situ cell screening methods and systems |
-
2022
- 2022-05-20 AU AU2022276537A patent/AU2022276537A1/en active Pending
- 2022-05-20 JP JP2023572225A patent/JP2024521142A/ja active Pending
- 2022-05-20 CA CA3220740A patent/CA3220740A1/en active Pending
- 2022-05-20 WO PCT/US2022/030370 patent/WO2022246275A1/en not_active Ceased
- 2022-05-20 US US18/561,259 patent/US20240263228A1/en active Pending
- 2022-05-20 EP EP22805631.3A patent/EP4352254A4/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024521142A (ja) | 2024-05-28 |
| EP4352254A1 (en) | 2024-04-17 |
| EP4352254A4 (en) | 2025-04-16 |
| CA3220740A1 (en) | 2022-11-24 |
| WO2022246275A1 (en) | 2022-11-24 |
| US20240263228A1 (en) | 2024-08-08 |
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