AU2022252299A1 - Compositions and methods for simultaneous genetic analysis of multiple libraries - Google Patents

Compositions and methods for simultaneous genetic analysis of multiple libraries Download PDF

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Publication number
AU2022252299A1
AU2022252299A1 AU2022252299A AU2022252299A AU2022252299A1 AU 2022252299 A1 AU2022252299 A1 AU 2022252299A1 AU 2022252299 A AU2022252299 A AU 2022252299A AU 2022252299 A AU2022252299 A AU 2022252299A AU 2022252299 A1 AU2022252299 A1 AU 2022252299A1
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Australia
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criterion
adaptor
disclosure
reads
kits
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AU2022252299A
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Inventor
Jiannan GUO
Carolyn Hutcheon
Mark Li
Lee Lim
Ira PEKKER
Chen-Hsun TSAI
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Resolution Bioscience Inc
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Resolution Bioscience Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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  • Chemical & Material Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
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  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2022252299A 2021-03-30 2022-03-30 Compositions and methods for simultaneous genetic analysis of multiple libraries Pending AU2022252299A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163167914P 2021-03-30 2021-03-30
US63/167,914 2021-03-30
US202263311041P 2022-02-16 2022-02-16
US63/311,041 2022-02-16
PCT/US2022/022640 WO2022212574A1 (en) 2021-03-30 2022-03-30 Compositions and methods for simultaneous genetic analysis of multiple libraries

Publications (1)

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AU2022252299A1 true AU2022252299A1 (en) 2023-10-19

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AU2022252299A Pending AU2022252299A1 (en) 2021-03-30 2022-03-30 Compositions and methods for simultaneous genetic analysis of multiple libraries

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US (1) US20240191293A1 (https=)
EP (1) EP4314340B1 (https=)
JP (1) JP2024512156A (https=)
KR (1) KR20240004397A (https=)
AU (1) AU2022252299A1 (https=)
BR (1) BR112023020082A2 (https=)
CA (1) CA3214221A1 (https=)
IL (1) IL307355A (https=)
WO (1) WO2022212574A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
WO2025228840A1 (en) * 2024-05-02 2025-11-06 Medicover Biotech Ltd. Method for somatic mutation detection
WO2025228816A1 (en) * 2024-05-02 2025-11-06 Medicover Biotech Ltd Method of targeted sequencing for diagnosis
US20260049302A1 (en) * 2024-08-14 2026-02-19 Sophia Genetics S.A. Systems and methods for assaying various regions of a genome at different resolutions

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
CN111254500B (zh) * 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) * 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
MX2019007444A (es) 2016-12-22 2019-08-16 Guardant Health Inc Metodos y sistemas para analisis de moleculas de acido nucleico.
WO2023282916A1 (en) * 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
EP4585697A3 (en) * 2020-07-30 2025-08-06 Guardant Health, Inc. Methods for isolating cell-free dna
EP4251765A1 (en) * 2020-11-30 2023-10-04 Guardant Health, Inc. Compositions and methods for enriching methylated polynucleotides

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Publication number Publication date
JP2024512156A (ja) 2024-03-18
US20240191293A1 (en) 2024-06-13
WO2022212574A1 (en) 2022-10-06
EP4314340A1 (en) 2024-02-07
IL307355A (en) 2023-11-01
EP4314340A4 (en) 2024-11-27
BR112023020082A2 (pt) 2024-01-23
EP4314340B1 (en) 2026-04-22
KR20240004397A (ko) 2024-01-11
CA3214221A1 (en) 2022-10-06

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