AU2021411520A1 - Methods and compositions for sequencing library preparation - Google Patents

Methods and compositions for sequencing library preparation Download PDF

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Publication number
AU2021411520A1
AU2021411520A1 AU2021411520A AU2021411520A AU2021411520A1 AU 2021411520 A1 AU2021411520 A1 AU 2021411520A1 AU 2021411520 A AU2021411520 A AU 2021411520A AU 2021411520 A AU2021411520 A AU 2021411520A AU 2021411520 A1 AU2021411520 A1 AU 2021411520A1
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AU
Australia
Prior art keywords
cases
stabilized
nucleic acid
sample
sequence
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Pending
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AU2021411520A
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English (en)
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AU2021411520A9 (en
Inventor
Marco BLANCHETTE
Elizabeth MUNDING
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Dovetail Genomics LLC
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Dovetail Genomics LLC
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Application filed by Dovetail Genomics LLC filed Critical Dovetail Genomics LLC
Publication of AU2021411520A1 publication Critical patent/AU2021411520A1/en
Publication of AU2021411520A9 publication Critical patent/AU2021411520A9/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2021411520A 2020-12-30 2021-12-29 Methods and compositions for sequencing library preparation Pending AU2021411520A1 (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US202063132304P 2020-12-30 2020-12-30
US63/132,304 2020-12-30
US202163145859P 2021-02-04 2021-02-04
US63/145,859 2021-02-04
US202163216170P 2021-06-29 2021-06-29
US63/216,170 2021-06-29
US202163217009P 2021-06-30 2021-06-30
US63/217,009 2021-06-30
US202163224722P 2021-07-22 2021-07-22
US63/224,722 2021-07-22
US202163281499P 2021-11-19 2021-11-19
US63/281,499 2021-11-19
PCT/US2021/065519 WO2022147129A1 (en) 2020-12-30 2021-12-29 Methods and compositions for sequencing library preparation

Publications (2)

Publication Number Publication Date
AU2021411520A1 true AU2021411520A1 (en) 2023-08-10
AU2021411520A9 AU2021411520A9 (en) 2025-01-16

Family

ID=82259726

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021411520A Pending AU2021411520A1 (en) 2020-12-30 2021-12-29 Methods and compositions for sequencing library preparation

Country Status (6)

Country Link
US (1) US20240084291A1 (https=)
EP (1) EP4271804A4 (https=)
JP (1) JP2024502028A (https=)
AU (1) AU2021411520A1 (https=)
CA (1) CA3203900A1 (https=)
WO (1) WO2022147129A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
KR102412442B1 (ko) 2016-05-13 2022-06-22 더브테일 제노믹스 엘엘씨 보존된 샘플로부터의 장범위 링키지 정보의 회수
CN119546775A (zh) * 2022-05-11 2025-02-28 多弗泰尔基因组学有限责任公司 用于测序文库制备的方法和组合物
WO2024233949A1 (en) * 2023-05-11 2024-11-14 Replace Therapeutics, Llc Revision of genetic material using direct replacement editing
CN121693564A (zh) 2023-06-26 2026-03-17 夏威夷大学 进化的整合酶和其用于基因组编辑的方法
CN118048436B (zh) * 2024-04-16 2024-06-21 中国农业科学院农业基因组研究所 用于微量细胞的靶向染色质互作捕获ULI-eHiChIP建库方法及应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201508985VA (en) * 2013-05-23 2015-12-30 Univ Leland Stanford Junior Transposition into native chromatin for personal epigenomics
SG11201807117WA (en) * 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
US20180080021A1 (en) * 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
EP3746566A1 (en) * 2018-01-31 2020-12-09 Dovetail Genomics, LLC Sample prep for dna linkage recovery
US20210332424A1 (en) * 2018-08-28 2021-10-28 10X Genomics, Inc. Methods of generating an array
US20220213469A1 (en) * 2019-04-30 2022-07-07 The Broad Institute, Inc. Methods and compositions for barcoding nucleic acid libraries and cell populations
EP4545965A3 (en) * 2019-06-27 2025-06-11 Dovetail Genomics, LLC Methods and compositions for proximity ligation

Also Published As

Publication number Publication date
US20240084291A1 (en) 2024-03-14
AU2021411520A9 (en) 2025-01-16
JP2024502028A (ja) 2024-01-17
EP4271804A1 (en) 2023-11-08
WO2022147129A1 (en) 2022-07-07
EP4271804A4 (en) 2024-12-04
CA3203900A1 (en) 2022-07-07

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