AU2021104968A4 - Method for preparing morchella culture - Google Patents
Method for preparing morchella culture Download PDFInfo
- Publication number
- AU2021104968A4 AU2021104968A4 AU2021104968A AU2021104968A AU2021104968A4 AU 2021104968 A4 AU2021104968 A4 AU 2021104968A4 AU 2021104968 A AU2021104968 A AU 2021104968A AU 2021104968 A AU2021104968 A AU 2021104968A AU 2021104968 A4 AU2021104968 A4 AU 2021104968A4
- Authority
- AU
- Australia
- Prior art keywords
- mixture
- culture
- oilseed rape
- preparing
- wheat berries
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 241000221638 Morchella Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 240000002791 Brassica napus Species 0.000 claims abstract description 24
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 24
- 241000209140 Triticum Species 0.000 claims abstract description 24
- 235000021307 Triticum Nutrition 0.000 claims abstract description 24
- 235000021028 berry Nutrition 0.000 claims abstract description 24
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 24
- 239000010902 straw Substances 0.000 claims abstract description 24
- 238000003860 storage Methods 0.000 claims abstract description 20
- 239000012634 fragment Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 11
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 230000003749 cleanliness Effects 0.000 claims abstract description 4
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 20
- 239000008188 pellet Substances 0.000 claims description 18
- 239000004743 Polypropylene Substances 0.000 claims description 6
- -1 polypropylene Polymers 0.000 claims description 6
- 229920001155 polypropylene Polymers 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 101100208473 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) lcm-2 gene Proteins 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 239000008247 solid mixture Substances 0.000 claims 3
- 239000001963 growth medium Substances 0.000 abstract description 19
- 241000894006 Bacteria Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 238000009630 liquid culture Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- 241000221700 Pezizales Species 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
Abstract
Disclosed is a method for preparing a morchella culture. The method comprises
the following preparation steps: SI, a preparation step: first, preparing high quality
wheat berries and oilseed rape straws, wherein it is unnecessary to ensure cleanliness
and sanitation of the wheat berries and oilseed rape straws; S2, taking out raw materials
and then taking 70-75% of oilseed rape straw fragments and 30-38% of wheat berries,
and meanwhile, ensuring that the sum of mass percent of the oilseed rape straw
fragments and the wheat berries is 100%; and S3, preparing a culture medium: putting
the oilseed rape straw fragments and the wheat berries in a culture bag, permeating the
oilseed rape straw fragments and the wheat berries with clear water, then adding
limewater with a concentration of 5% after permeation and draining off the clear water
and limewater in the culture bag after being placed for 20-30 minutes. The method
improves a sterilizing effect effectively. A culture is packaged by an airtight storage
bottle, such that the culture can be prevented from being polluted by external bacteria
effectively, and meanwhile bacteria can be killed more efficiently for 15-20 hours, such
that a using effect of the culture is improved, and the culture is high in practicality and
remarkable in progressiveness.
1/1
Preparation step
Raw material take out
S2
Culture medium S
nrenaration S
Sealing
S
Disinfection S
on S5
tReexaminati
Liquid cultivation S
Solid cultivation
Fi. IS8
Fig. 1
Description
1/1
Preparation step
Raw material take out
Culture medium nrenaration SS
Sealing S S2 Disinfection S on S5
tReexaminati
Liquid cultivation S
Solid cultivation Fi. IS8
Fig. 1
TECHNICAL FIELD The present invention relates to a method for preparing a morchella culture and in
particular to a method for preparing a morchella culture.
Morchella is a valuable and rare edible mushroom affiliated to morchella,
morohellaceae, pezizale, ascomycetes, eumycetes. Morchella is delicious in taste, high
in nutritional value and rich in essential amino acids for human body. However, after
the existing morchella culture is prepared at present, in particular, in a transportation
process, a risk of deterioration exists. Its major reason is that bacterial breeding is
caused to lead to rot as the morchella culture is not extincted thoroughly. Thus, it is
necessary to improve the condition.
It is thereof an objective of the present invention to provide a method for preparing
a morchella culture to solve the problem in the background art.
In order to achieve the object, the present invention provides a technical scheme
as follows:
A method for preparing a morchella culture, including the following preparation
steps:
Sl, a preparation step: first, preparing high quality wheat berries and oilseed rape
straws, wherein it is unnecessary to ensure cleanliness and sanitation of the wheat
berries and oilseed rape straws;
S2, taking out raw materials and then taking 70-75% of oilseed rape straw
fragments and 30-38% of wheat berries, and meanwhile, ensuring that the sum of mass
percent of the oilseed rape straw fragments and the wheat berries is 100%;
S3, preparing a culture medium: putting the oilseed rape straw fragments and the
wheat berries in a culture bag, permeating the oilseed rape straw fragments and the
wheat berries with clear water, then adding limewater with a concentration of 5% after
permeation, draining off the clear water and limewater in the culture bag after being
placed for 20-30 minutes, then putting the culture bag in a high-temperature disinfection
box and sterilizing the culture bag, taking out the culture bag after sterilization and
taking out a mixture in the culture bag, then taking out a half of the mixture and drying
the same, and drying the half of the mixture to obtain a solid culture medium and
blending water into the other half of the mixture to obtain a liquid culture medium;
S4, sealing: respectively putting the obtained solid culture medium and liquid
culture medium in storage bottles, and meanwhile, sealing bottle openings with 20cm
polypropylene films and fixing the polypropylene film seals by way of winding
peripheries of the bottle openings by thread ropes;
S5, disinfection: putting the obtained storage bottles at 110-120°C and sterilizing
the storage bottles for 15-20 hours, then cooling the storage bottles with cold water, and
conducting a next step after cooling the storage bottles to room temperature;
S6, reexamination: uncovering the storage bottles, putting the satirized liquid
culture medium and solid culture medium in an incubator and culturing the same for 1
2 days, wherein it is ensured that sterilization is thorough in case of sterile growth and
sterilization is halfway in case of non-sterile growth, and repeating the steps in the S4
till reexamination is conducted successively;
S7, liquid cultivation: inoculating 1-3 slices of lcm2 morchella stock cultures to
the liquid culture first, conducting static culture for 24-28 hours under a 18-24°C
constant temperature nonluminous condition at the same time, and then conducting
further cultivation for 5-6 days in an environment of a rotating speed of 120-140 r/min
to obtain a seed material A; and
S8, conducting fixed cultivation, then inoculating 2-4 slices of morchella stock
cultures to the solid culture medium and conducting constant temperature cultivation for 5-10 days at 20-26°C to obtain a seed material B, then comparing quantities of mycelium pellets in the seed material A and the seed material B, taking out the seed material with great quantity of mycelium pellets as a culture, and conducting further cultivation for 2-3 days on the seed material with small quantity of mycelium pellets, and then using the seed material as the culture.
In order to ensure the quality, the present invention is improved such that in the
step S3, after 5-6 days of further cultivation, it is necessary to ensure that the quantity
of mycelium pellets reaches 2800-3500/mL, and if no, further cultivation is conducted
and if yes, a next step is continued.
In order to improve the cultivation efficiency, the present invention is improved
such that in the step S7, an illumination in the constant temperature nonluminous
environment shall be smaller than 0.02Lux.
In order to improve the disinfection effect, the present invention is improved such
that in the step S3, a sterilizing temperature of the high temperature disinfection box
ranges from 130°C to 140°C and a sterilizing time of the high temperature disinfection
box ranges from 1.5 hours to 2.5 hours.
In order to ensure a sterile environment, the present invention is improved such
that in the step S5, the room temperature ranges from 20°C to 30°C, and in the step S6,
a condition of sterile growth is that the number of colonies is smaller than or equal to
1OOcfu/g.
Compared with the prior art, the present invention has the following beneficial
effects:
Compared with a conventional preparation method, the method for preparing the
morchella culture improves the sterilizing effect effectively. A culture is packaged by
an airtight storage bottle, such that the culture can be prevented from being polluted by
external bacteria effectively, and meanwhile bacteria can be killed more efficiently for
-20 hours. A part of culture medium containing bacteria may be found via the
reexamination step, such that the sterilizing effect is further improved. Meanwhile, the culture with more mycelium pellets can be screened by way of comparing the mycelium pellet quantity, such that the using effect of the culture is improved, and the culture is high in practicality and remarkable in progressiveness.
Other characteristics and advantages of the embodiments of the present invention
will be described in the follow-up description, and become obvious from the description
partially or are understood by implementing the embodiments of the present
invention.Object and other advantages of the present invention can be realized and
gained by structures indicated in the description, claims and drawings.
Further detailed description of the technical scheme of the present invention will
be made below in combination with the drawings and the embodiments.
In order to describe the embodiments of the present invention or the technical
scheme in the prior art more clearly, brief introduction on drawings needed to be used
in the embodiment will be made below. It is obvious that the drawings described below
are some embodiments of the present invention, and those skilled in the technical field
further can obtain other drawings according to the drawings without creative efforts.
Fig. 1 is a step diagram of a method for preparing a morchella culture provided by
the present invention.
Clear and intact description will be made on technical scheme in the embodiment
of the present invention below in combination with drawings in the embodiment of the
present invention. The described embodiments are merely a part of embodiments of the
present invention and are not all the embodiments. On a basis of the embodiments in
the present invention, all other embodiments obtained by those skilled in the technical
field without creative efforts fall into the scope of protection of the present invention.
Example I of the present invention: A method for preparing a morchella culture,
including the following preparation steps:
Sl, a preparation step: first, high quality wheat berries and oilseed rape straws are
prepared, wherein it is unnecessary to ensure cleanliness and sanitation of the wheat
berries and oilseed rape straws;
S2, raw materials are taken out and then 70-75% of oilseed rape straw fragments
and 30-38% of wheat berries are taken, and meanwhile, a condition that the sum of
mass percent of the oilseed rape straw fragments and the wheat berries is 100% is
ensured;
S3, a culture medium is prepared: the oilseed rape straw fragments and the wheat
berries are put in a culture bag, the oilseed rape straw fragments and the wheat berries
are permeated with clear water, then limewater is added with a concentration of 5%
after permeation, the clear water and limewater are drained off in the culture bag after
being placed for 20-30 minutes, then the culture bag is put in a high-temperature
disinfection box and the culture bag is sterilized, the culture bag after sterilization is
taken out and a mixture in the culture bag is taken out, then a half of the mixture is
taken out and the same is dried, and the half of the mixture is dried to obtain a solid
culture medium and water is blended into the other half of the mixture to obtain a liquid
culture medium;
S4, sealing: the obtained solid culture medium and liquid culture medium are
respectively put in storage bottles, and meanwhile, bottle openings are sealed with
cm polypropylene films and the polypropylene film seals are fixed by way of winding
peripheries of the bottle openings by thread ropes;
S5, disinfection: the obtained storage bottles are put at 110-120°C and sterilized
for 15-20 hours, then the storage bottles are cooled with cold water, and a next step is
conducted after cooling the storage bottles to room temperature;
S6, reexamination: the storage bottles are uncovered, the satirized liquid culture
medium and solid culture medium are put in an incubator and the same is cultured for
1-2 days, wherein it is ensured that sterilization is thorough in case of sterile growth
and sterilization is halfway in case of non-sterile growth, and repeating the steps in the
S4 till reexamination is conducted successively;
S7, liquid cultivation: 1-3 slices of lcm2 morchella stock cultures are inoculated
to the liquid culture first, static culture is conducted for 24-28 hours under a 18-24°C
constant temperature nonluminous condition at the same time, and then further
cultivation is conducted for 5-6 days in an environment of a rotating speed of 120-140
r/min to obtain a seed material A; and
S8, fixed cultivation is conducted, then 2-4 slices of morchella stock cultures are
inoculated to the solid culture medium and constant temperature cultivation is
conducted for 5-10 days at 20-26°C to obtain a seed material B, then quantities of
mycelium pellets in the seed material A and the seed material B are compared, the seed
material with great quantity of mycelium pellets is taken out as a culture, and further
cultivation is conducted for 2-3 days on the seed material with small quantity of
mycelium pellets, and then the seed material used as the culture.
In the embodiment, in the step S3, after 5-6 days of further cultivation, it is
necessary to ensure that the quantity of mycelium pellets reaches 2800-3500/mL, and
if no, further cultivation is conducted and if yes, a next step is continued. Thus, it is
ensured that the quantities of mycelium pellets are maintained at a higher level all the
time, such that the method is higher in practicality.
In the embodiment, in the step S7, an illumination in the constant temperature
nonluminous environment shall be smaller than 0.02Lux. As the illumination is smaller
than 0.02Lux, the environment is relatively dark, which is conductive to growth of
mycelium pellets. Morchella is a shade-loving culture. The dark environment is
beneficial for growth and development of fruiting bodies of morchella.
In the embodiment, in the step S3, a sterilizing temperature of the high temperature
disinfection box ranges from 130°C to 140°C and a sterilizing time of the high
temperature disinfection box ranges from 1.5 hours to 2.5 hours. The high temperature of 130°C to 140°C may kill most bacteria and the sterilizing time of 1.5-2.5 hours may ensure the sterilizing quality effectively.
In the embodiment, in the step S5, the room temperature ranges from 20°C to 30°C,
and in the step S6, a condition of sterile growth is that the number of colonies is smaller
than or equal to 100cfu/g. 100 bacterial cells per ml may meet the sterile condition,
thereby inhibiting bacterial breeding effectively.
It may be seen from the embodiment that compared with a conventional
preparation method, the method for preparing the morchella culture improves the
sterilizing effect effectively. A culture is packaged by an airtight storage bottle, such
that the culture can be prevented from being polluted by external bacteria effectively,
and meanwhile bacteria can be killed more efficiently for 15-20 hours. A part of culture
medium containing bacteria may be found via the reexamination step, such that the
sterilizing effect is further improved. Meanwhile, the culture with more mycelium
pellets can be screened by way of comparing the mycelium pellet quantity, such that
the using effect of the culture is improved, and the culture is high in practicality and
remarkable in progressiveness.
Although detailed description is made on the present invention with reference to
the embodiment, those skill in the art still can modify the technical schemes recorded
by the embodiments or replace part of technical characteristics equivalently. Any
modification, equivalent substitution, improvement and the like made within the spirit
and principle of the present invention shall fall within the scope of protection of the
prevent invention.
Claims (4)
1. A method for preparing a morchella mixture, characterized by comprising the
following preparation steps:
Sl, a preparation step: first, preparing high quality wheat berries and oilseed rape
straws, wherein it is unnecessary to ensure cleanliness and sanitation of the wheat
berries and oilseed rape straws;
S2, taking out raw materials and then taking 70-75% of oilseed rape straw
fragments and 30-38% of wheat berries, and meanwhile, ensuring that the sum of mass
percent of the oilseed rape straw fragments and the wheat berries is 100%;
S3, preparing a mixture: putting the oilseed rape straw fragments and the wheat
berries in a mixture bag, permeating the oilseed rape straw fragments and the wheat
berries with clear water, then adding limewater with a concentration of 5% after
permeation, draining off the clear water and limewater in the culture bag after being
placed for 20-30 minutes, then putting the mixture bag in a high-temperature
disinfection box and sterilizing the mixture bag, taking out the mixture bag after
sterilization and taking out a mixture in the mixture bag, then taking out a half of the
mixture and drying the same, and drying the half of the mixture to obtain a solid mixture
and blending water into the other half of the mixture to obtain a liquid mixture;
S4, sealing: respectively putting the obtained solid mixture and liquid mixture in
storage bottles, and meanwhile, sealing bottle openings with 20cm polypropylene films
and fixing the polypropylene film seals by way of winding peripheries of the bottle
openings by thread ropes;
S5, disinfection: putting the obtained storage bottles at 110-120°C and sterilizing
the storage bottles for 15-20 hours, then cooling the storage bottles with cold water, and
conducting a next step after cooling the storage bottles to room temperature;
S6, reexamination: uncovering the storage bottles, putting the satirized liquid
mixture and solid mixture in an incubator and observing the same for 1-2 days, wherein
it is ensured that sterilization is thorough in case of sterile growth and sterilization is halfway in case of non-sterile growth, and repeating the steps in the S4 till reexamination is conducted successively;
S7, liquid observation: placing 1-3 slices of lcm2 morchella stock mixture to the
liquid mixture first, conducting static observation for 24-28 hours under a 18-24°C
constant temperature nonluminous condition at the same time, and then conducting
further observation for 5-6 days in an environment of a rotating speed of 120-140 r/min
to obtain a seed material A; and
S8, conducting fixed observation, then placing 2-4 slices of morchella stock
mixtures to the solid culture mixture and conducting constant temperature observation
for 5-10 days at 20-26°C to obtain a seed material B, then comparing quantities of
mycelium pellets in the seed material A and the seed material B, taking out the seed
material with great quantity of mycelium pellets as a mixture, and conducting further
mixing for 2-3 days on the seed material with small quantity of mycelium pellets, and
then using the seed mixture as the culture.
2. The method for preparing a morchella mixture according to claim 1,
characterized in that in the step S3, after 5-6 days of further observation, it is necessary
to ensure that the quantity of mycelium pellets reaches 2800-3500/mL, and if no, further
observation is conducted and if yes, a next step is continued.
3. The method for preparing a morchella mixture according to claim 1,
characterized in that in the step S7, an illumination in the constant temperature
nonluminous environment shall be smaller than 0.02Lux.
4. The method for preparing a morchella mixture according to claim 1,
characterized in that in the step S3, a sterilizing temperature of the high temperature
disinfection box ranges from 130°C to 140°C and a sterilizing time of the high
temperature disinfection box ranges from 1.5 hours to 2.5 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104968A AU2021104968A4 (en) | 2021-08-05 | 2021-08-05 | Method for preparing morchella culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104968A AU2021104968A4 (en) | 2021-08-05 | 2021-08-05 | Method for preparing morchella culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021104968A4 true AU2021104968A4 (en) | 2021-09-30 |
Family
ID=77857768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021104968A Ceased AU2021104968A4 (en) | 2021-08-05 | 2021-08-05 | Method for preparing morchella culture |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2021104968A4 (en) |
-
2021
- 2021-08-05 AU AU2021104968A patent/AU2021104968A4/en not_active Ceased
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| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |